Supplementation of sulfate polysaccharides in the seminal cooling medium of common curimata (Prochilodus brevis).

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2023-07-01 DOI:10.54680/fr23410110512

Y. S. Sales, Jéssica SalesLobato, Carla Tatiana Nascimento Sousa Vieira, João Eudes Faria Cavalcante Filho, Yasmim Maia Ferreira, Marcos Luiz da Silva Apoliano, R. V. Nascimento, Silvio Alencar Căndido Sobrinho, José Ariévilo Gurgel Rodrigues, Carla Pamela Braga Guia, Fernanda Vitória Almeida Magalhães, C. Salmito‐Vanderley

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引用次数: 0

Abstract

BACKGROUND The use of sulfated polysaccharides (PS) in seminal cooling is known to improve seminal quality. OBJECTIVE To evaluate the effect of different concentrations of PS, extracted from the macroalgae Gracilaria domigensis as a supplement to the seminal cooling medium of the reophilic fish Prochilodus brevis (common curimatã). MATERIALS AND METHODS Five semen pools were diluted in ACP-104 (treatment T1), in BTS® (T2) and in BTS® with different concentrations of PS (0.5 [T3]; 1.0 [T4] and 1.5 [T5]). The samples were cooled for different times (0, 6, 24, 48, 72, 96 and 120 h) and after each hour they were analyzed for: morphology, membrane integrity, DNA integrity and sperm kinetics. RESULTS There were no significant differences between the treatments containing different concentrations of sulfated polysaccharides. Regarding the different cooling times, it was possible to observe that after hour 96, there was a reduction in the parameters of sperm kinetics. For DNA integrity there was no significant difference in relation to the treatments nor in relation to the hours. For membrane integrity, a reduction was noted as of hour 96, but there was no influence of polysaccharides. For the sperm morphology, there was no statistical difference between the hours, however the BTS was better than the ACP-104. CONCLUSION It is concluded that the use of polysaccharides in seminal cooling has no negative effect on sperm parameters and proves that seminal cooling keeps the material viable for up to 72 hours. Doi: 10.54680/fr23410110512.

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枸杞种子冷却液中添加硫酸多糖的研究。

背景硫酸盐多糖(PS)用于种子冷却可以改善种子质量。目的评价从大藻中提取的不同浓度的PS作为嗜水鱼类短尾蛇(Prochilodus brevis, curimatã)种子冷却介质的补充效果。材料与方法5个精液池分别稀释ACP-104 (T1)、BTS®(T2)和不同浓度PS (0.5 [T3])的BTS®;1.0 [T4]和1.5 [T5])。样品冷却不同时间(0、6、24、48、72、96和120 h)，每小时后进行形态学、膜完整性、DNA完整性和精子动力学分析。结果不同浓度硫酸多糖处理间无显著差异。关于不同的冷却时间，可以观察到，96小时后，精子动力学参数有所降低。对于DNA完整性，与处理和时间没有显著差异。对于膜的完整性，在96小时时发现了降低，但多糖没有影响。在精子形态方面，两个小时之间没有统计学差异，但BTS优于ACP-104。结论多糖冷却精子对精子参数无负面影响，可使精子在72小时内保持活性。Doi: 10.54680 / fr23410110512。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.