Strip pulled straw: cost effective alternative cryodevice for the vitrification of oocytes.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2023-07-01 DOI:10.54680/fr23410110212

Khursheed Ahmad Sofi, Beenish Qureshi

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引用次数: 0

Abstract

BACKGROUND Increased cooling and warming rates by using a suitable cryodevice allows the use of lower cryoprotectant concentration and reduces cryoinjuries as a result of the rapid passage through the 'dangerous' temperature zone. OBJECTIVE To evaluate the effectiveness of newly customized strip pulled straw (SPS) with respect to post warming quality, viability, and in vitro maturation for immature oocytes post-vitrification of. MATERIALS AND METHODS SPS was prepared using conventional French mini straw to combine the merits of OPS and the Cryotop system. Immature sheep oocytes were treated in 15% EG + 15% DMSO, loaded on SPS and plunged into liquid nitrogen (LN). Post thaw quality, viability, and maturation rates of oocytes were determined after 1 week storage in LN. RESULTS SPS achieved a post-thaw morphological survival of 90.9% with 9.0% morphological defects, 96.4% viability and 51% in vitro maturation. In comparison to OPS, SPS had higher post thaw survival (86.5% vs 67.9%) and maturation rate (57.7% vs 50.5%) with lower morphological defects (13.5% vs 32.1%). Cumulus cell loss was the highest among morphological abnormalities of post warm oocytes in SPS (40.9%) and OPS (44.1%). The data showed acceptable post thaw survival, viability and in vitro maturation rate of immature ovine oocytes using SPS as compared to traditional OPS. CONCLUSION SPS can be used as a cost effective alternative device for oocyte vitrification. Doi: 10.54680/fr23410110212.

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条状吸管:成本效益高的卵母细胞玻璃化冷冻装置。

背景:通过使用合适的低温装置来提高冷却和升温速率，可以使用较低的低温保护剂浓度，并减少由于快速通过“危险”温度区域而造成的低温损伤。目的评价新型定制条拉吸管(SPS)对玻璃化后未成熟卵母细胞温后质量、活力和体外成熟的影响。材料与方法结合OPS与Cryotop系统的优点，采用传统的法式迷你吸管制备ssps。用15% EG + 15% DMSO处理未成熟绵羊卵母细胞，负载SPS，注入液氮(LN)。解冻后卵母细胞的质量、活力和成熟率在LN中保存1周后测定。结果sps解冻后形态存活率为90.9%，形态缺陷率为9.0%，存活率为96.4%，体外成熟率为51%。与OPS相比，SPS具有更高的解冻后存活率(86.5% vs 67.9%)和成熟率(57.7% vs 50.5%)，形态学缺陷(13.5% vs 32.1%)更低。温后卵母细胞形态异常以积云细胞丢失最多，分别为40.9%和44.1%。数据显示，与传统的OPS相比，使用SPS的未成熟绵羊卵母细胞解冻后存活率、活力和体外成熟率均可接受。结论sps是一种经济有效的卵母细胞玻璃化冷冻技术。Doi: 10.54680 / fr23410110212。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.