马精液的冷冻受到暴露时间和冷冻保护剂甘油浓度的影响。

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2023-07-01 DOI:10.54680/fr23410110412

Pablo Luis Fracaro, C. Corcini, Norton Luis Souza Gatti, Jorge Squeff Filho, I. B. Acosta, Felipe Pires Hartwig, Bruna Da Rosa Curcio, A. S. V. Junior

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引用次数: 0

摘要

甘油是一种冷冻保护剂，广泛用于哺乳动物精液的冷冻，但没有研究表明其最佳浓度和合适的暴露时间对马物种。目的探讨冷冻保护剂甘油的浓度(2、3、4、5%)与暴露时间(15、30、45、60、75、90 min)对马精液冷冻成功率的影响。材料与方法对12头公马的精液进行不同甘油浓度、不同暴露时间的冷冻处理。用计算机辅助精液分析(CASA)系统评估解冻精子的动力学参数，用流式细胞术评估细胞特征参数。结果考虑到精子的总运动和渐进运动，我们得出结论，5%甘油暴露15和30分钟，4%甘油暴露45分钟和3%甘油暴露90分钟的方案产生了最好的结果。结论我们建议使用这些方法来更好地冷冻保存马精液。Doi: 10.54680 / fr23410110412。

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Freezing of equine semen is influenced by exposure time and concentration of the cryoprotectant glycerol.

BACKGROUND Glycerol is a cryoprotectant widely used in the freezing of mammalian semen, but no study has demonstrated its optimum concentration and the appropriate exposure time for equine species. OBJECTIVE To demonstrate that the exposure time (15, 30, 45, 60, 75 and 90 min) versus concentration (2, 3, 4 and 5%) of the cryoprotectant glycerol influences the freezing success of equine semen. MATERIALS AND METHODS The ejaculate of 12 stallions were frozen in different glycerol concentrations following different exposure times. The thawed sperm was evaluated for kinetic parameters using a Computer Assisted Semen Analysis (CASA) system and cell feature parameters were assessed by flow cytometry. RESULTS Considering the total and progressive motility of the spermatozoa, we concluded that protocols using 5% glycerol for 15 and 30 min exposure, 4% glycerol for 45 min exposure and 3% glycerol for 90 min exposure generated the best results. CONCLUSION We suggest the use of any of these protocols for a better cryopreservation of equine semen. Doi: 10.54680/fr23410110412.

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.