Supplementation of sulfate polysaccharides in the seminal cooling medium of common curimata (Prochilodus brevis).

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2023-07-01

Y S Sales, J S Lobato, C T Nascimento Sousa Vieira, J E Faria Cavalcante Filho, Y M Ferreira, M L da Silva Apoliano, R V do Nascimento, S A Candido Sobrinho, J A Gurgel Rodrigues, C P Braga Guia, F V Almeida Magalhaes, C S Salmito-Vanderley

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引用次数: 0

Abstract

Background: The use of sulfated polysaccharides (PS) in seminal cooling is known to improve seminal quality.

Objective: To evaluate the effect of different concentrations of PS, extracted from the macroalgae Gracilaria domigensis as a supplement to the seminal cooling medium of the reophilic fish Prochilodus brevis (common curimatã).

Materials and methods: Five semen pools were diluted in ACP-104 (treatment T1), in BTS® (T2) and in BTS® with different concentrations of PS (0.5 [T3]; 1.0 [T4] and 1.5 [T5]). The samples were cooled for different times (0, 6, 24, 48, 72, 96 and 120 h) and after each hour they were analyzed for: morphology, membrane integrity, DNA integrity and sperm kinetics.

Results: There were no significant differences between the treatments containing different concentrations of sulfated polysaccharides. Regarding the different cooling times, it was possible to observe that after hour 96, there was a reduction in the parameters of sperm kinetics. For DNA integrity there was no significant difference in relation to the treatments nor in relation to the hours. For membrane integrity, a reduction was noted as of hour 96, but there was no influence of polysaccharides. For the sperm morphology, there was no statistical difference between the hours, however the BTS was better than the ACP-104.

Conclusion: It is concluded that the use of polysaccharides in seminal cooling has no negative effect on sperm parameters and proves that seminal cooling keeps the material viable for up to 72 hours. Doi: 10.54680/fr23410110512.

本刊更多论文

短柱原球茎种子冷却培养基中硫酸多糖的补充。

背景：众所周知，在精液冷却中使用硫酸多糖（PS）可以改善精液质量。目的：评价从大型藻类龙须菜（Gracilia domigensis）中提取的不同浓度的PS作为对嗜热鱼短原体（Prochilodus brevis，common curimatã）精液冷却介质的补充的效果。材料和方法：在ACP-104（处理T1）中稀释5个精液池，在BTS®（T2）和具有不同PS浓度（0.5[T3]；1.0[T4]和1.5[T5]）的BTS®中。将样品冷却不同时间（0、6、24、48、72、96和120小时），每小时后对其进行形态学、膜完整性、DNA完整性和精子动力学分析。结果：不同浓度的硫酸多糖处理之间无显著差异。关于不同的冷却时间，可以观察到在96小时后，精子动力学参数降低。就DNA完整性而言，与处理时间和时间无关，没有显著差异。对于膜完整性，在96小时时注意到减少，但没有多糖的影响。在精子形态方面，两个小时之间没有统计学差异，但BTS比ACP-104好。结论：在精液冷却中使用多糖对精子参数没有负面影响，并证明精液冷却可使材料存活72小时。Doi:10.54680/fr23410110512。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.