Cryo letters最新文献_第7页

Cryopreservation of Lilium candidum germplasm: analysis of pre- and post-freeze treatments. 念珠百合种质的冷冻保存：冷冻前和冷冻后处理分析。

IF 1 4区生物学

Cryo letters Pub Date : 2023-09-01 DOI: 10.54680/fr23510110512

Hilal Büşra Tokgöz, Hakan Karakaş, Ergun Kaya, Hasan Yildirim, Ademi Fahri Pi̇rhan, F. Altan

{"title":"Cryopreservation of Lilium candidum germplasm: analysis of pre- and post-freeze treatments.","authors":"Hilal Büşra Tokgöz, Hakan Karakaş, Ergun Kaya, Hasan Yildirim, Ademi Fahri Pi̇rhan, F. Altan","doi":"10.54680/fr23510110512","DOIUrl":"https://doi.org/10.54680/fr23510110512","url":null,"abstract":"BACKGROUND Lilium candidum L. is a perennial ornamental plant that has various medicinal properties and is used in the cosmetic industry. The species is facing threats from urbanization and climate change and requires urgent protection. The most secure and efficient technology for the long-term storage of plant genetic resources is cryopreservation, which involves preserving genetic material at extremely low temperatures. OBJECTIVE Today, plant biodiversity is endangered because of the narrowing of its natural distribution areas and/or destruction for different purposes. This study concentrated on creating a cryopreservation process using shoot tips and calluses as explant sources for the long-term conservation of L. candidum species. MATERIALS AND METHODS Populations of L. candidum naturally distributed from three different regions of Turkey (Kepsut, Balikesir; the area surrounding Bafa Lake, Aydin; and Fethiye-Mugla) were grown in vitro to supply shoot tip and callus explants. Prior to freezing by droplet-vitrification and vitrification techniques, shoot tips and calluses were treated with MS nutritional medium supplemented with 0.4 M sucrose 7 g per L agar and plant vitrification solution 2 (PVS2). RESULTS Cryopreserved shoot tips showed the highest levels of regeneration (71.8%) after a PVS2 treatment of 90 min, while calluses showed the highest levels of regrowth (63.9%) after a PVS2 exposure of 60 min. CONCLUSION High levels of regrowth are produced when the various cryopreservation procedures described here are used to preserve both shoot tip and callus explants. This potentially makes the method promising for the long-term preservation of endangered L. candidum varieties. Doi.org/10.54680/fr23510110512.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"11 1","pages":"263-273"},"PeriodicalIF":1.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139344799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning, expression, purification and functional study of low-temperature chitinase PBCHI5 gene from marine-derived photobacteria. 海洋光细菌低温几丁质酶 PBCHI5 基因的克隆、表达、纯化和功能研究。

IF 1 4区生物学

Cryo letters Pub Date : 2023-09-01 DOI: 10.54680/fr23510110212

J Wu, Y. Liu, J. Zhang, X. Wang

{"title":"Cloning, expression, purification and functional study of low-temperature chitinase PBCHI5 gene from marine-derived photobacteria.","authors":"J Wu, Y. Liu, J. Zhang, X. Wang","doi":"10.54680/fr23510110212","DOIUrl":"https://doi.org/10.54680/fr23510110212","url":null,"abstract":"BACKGROUND Chitin is the second largest carbon source on the earth, and chitosan oligosaccharides produced by its degradation have good application prospects in medicine, cosmetics, and agricultural production. OBJECTIVE The discovery of a chitinase with high efficiency, high stability and clear degradation mechanism is of great help to promote the research of chitin derivatives and the development of the industrial chain. MATERIALS AND METHODS In this experiment, a low-temperature chitinase-producing strain Photobacterium sp. LG-29 was isolated from deep-sea mud in the Bohai Sea, and studied by means of molecular biology, biochemistry and bioinformatics. RESULTS Purification of chitinase yielded an enzyme solution with a concentration of 0.918 mg per mL and a specific activity of 21.036 U per mg. The optimum action temperature is 35 degree C, and it is still active at 4 degree C, showing low-temperature enzymatic activity, and also has certain thermal stability. The optimum pH is 8.0, and it maintains more than 70% of the enzyme activity at pH 11, which is very stable in an alkaline environment. Mn2+, Ca2+, and Mg2+ are the main activators of enzymes, while Fe2+, Zn2+, etc. have extremely significant inhibitory effects on enzymes. The Km and Kcat of chitinase were determined to be 269.05 μmol/L and 0.49 min-1, respectively. Chitinase PbCHI5 has both endonuclease and exonuclease activity. The theoretical pI of the enzyme is 4.16, which is a stable hydrophilic protein. CONCLUSION This experiment laid a theoretical foundation for the development and utilization of new low-temperature chitinases. Doi.org/10.54680/fr23510110212.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"23 1","pages":"280-290"},"PeriodicalIF":1.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139346136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of paclitaxel before vitrification on the nuclear maturation and development of immature porcine oocytes. 玻璃化前紫杉醇对未成熟猪卵母细胞核成熟和发育的影响。

IF 1 4区生物学

Cryo letters Pub Date : 2023-09-01

M Tone, R Ukyo, S H Sakamoto, K Hemmi, I Kokayashi, Y Tsuzuki

{"title":"Effects of paclitaxel before vitrification on the nuclear maturation and development of immature porcine oocytes.","authors":"M Tone, R Ukyo, S H Sakamoto, K Hemmi, I Kokayashi, Y Tsuzuki","doi":"","DOIUrl":"","url":null,"abstract":"Background: Cryopreservation of porcine oocytes is difficult compared with other species and immature oocytes particularly so compared to the meiotic stage.Objective: To evaluate the efficacy of a pretreatment with 1 micromole per L paclitaxel (PTX, 30 min exposure) before vitrification to promote the maturation of porcine immature oocytes.Materials and methods: Cumulus cell-enclosed oocytes (COs) aspirated from porcine ovaries were divided into three groups: i) non-pretreated with PTX and non-vitrified group (control group); ii) pretreated with PTX and vitrified group (PTX-V group); and iii) non-pretreated with PTX and vitrified group (nPTX-V group).Results: The nuclear maturation rate up to the preovulatory stage was significantly lower (P < 0.05) in the nPTX-V group than in the control group, but was similar in the PTX-V and control groups. No significant differences were observed in viability assessed by a normal CO morphology and the embryonic development of oocytes activated by the parthenogenetic stimulation between the PTX-V and control groups, but not the non-PTX-V group.Conclusion: PTX may promote the maturation of vitrified porcine immature oocytes. Doi.org/10.54680/fr23510110812.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"44 5","pages":"307-313"},"PeriodicalIF":1.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138458431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced heat transfer by medical gauze for cell vitrification with French straw. 用医用纱布法吸管玻璃化细胞增强传热。

IF 1 4区生物学

Cryo letters Pub Date : 2023-09-01

S Tao, B Liu

引用次数: 0

Enhanced heat transfer by medical gauze for cell vitrification with French straw. 利用医用纱布增强热传导，实现法国秸秆细胞玻璃化。

IF 1 4区生物学

Cryo letters Pub Date : 2023-09-01 DOI: 10.54680/fr23510110312

Tao Song, Baolin Liu

引用次数: 0

In vitro and in vivo effect of oxytetracycline on sperm parameters in breeding rooster. 土霉素对种公鸡精子参数的体内外影响

IF 1 4区生物学

Cryo letters Pub Date : 2023-09-01 DOI: 10.54680/fr23510110412

Linda Mohammedi, Ahmed Messai, L. Touazi, M. Iguer-Ouada

{"title":"In vitro and in vivo effect of oxytetracycline on sperm parameters in breeding rooster.","authors":"Linda Mohammedi, Ahmed Messai, L. Touazi, M. Iguer-Ouada","doi":"10.54680/fr23510110412","DOIUrl":"https://doi.org/10.54680/fr23510110412","url":null,"abstract":"BACKGROUND Some antimicrobials could adversely affect sperm quality during sperm cryopreservation and antibiotic treatment with subsequent effects on fertility outputs. To our knowledge, no similar studies have been conducted on breeding roosters, especially for oxytetracycline (OTC). OBJECTIVE To investigate both in vitro and in vivo impact of oxytetracycline on sperm parameters in breeding roosters. METHODS Sperm motility parameters were objectively analyzed using the CASA system including total motility (TM %), progressive motility (PM %), all sperm velocities, the sperm count, and cell viability during 9 days of in vivo treatment. In the in vitro investigation, the pooled sperm was diluted and divided into a control aliquot (diluted in 0.9% NaCl) and treated samples. Motility parameters were assessed after 0, 1, 2, 3, 4, 5, and 6 hours of storage at 37ºC. In the in vivo study, 1 g per L of OTC was administrated to five individuals for nine consecutive days. Fresh semen samples were analyzed at T0 (before treatment) and after 6 (T6) and 9 days (T9) of treatment. RESULTS OTC caused significant impairment of sperm quality in vivo. A drastic reduction in sperm concentration, viability, TM, PM, and all kinematic parameters was observed after 6 days of treatment. However, at day 9 sperm quality had improved to be nearly similar to T0. In vitro, OTC induced similar sperm impairment on all sperm motility parameters. CONCLUSION Oxytetracycline exhibited negative effects on rooster sperm both in vivo and in vitro and appears consequently not suitable in cryopreservation extenders. Doi.org/10.54680/fr23510110412.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"100 1","pages":"291-298"},"PeriodicalIF":1.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139346952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PERSPECTIVE: Potential and reality of cryopreserving somatic cells of wild felids for conservation. 展望:野生猫科动物体细胞低温保存的潜力与现实。

IF 1 4区生物学

Cryo letters Pub Date : 2023-09-01

L L Vieira Rodrigues, A F Pereira

{"title":"PERSPECTIVE: Potential and reality of cryopreserving somatic cells of wild felids for conservation.","authors":"L L Vieira Rodrigues, A F Pereira","doi":"","DOIUrl":"","url":null,"abstract":"The loss of biodiversity caused by anthropogenic actions is also a reality for the members of the Felidae family. Except for the domestic cat, all felid species have some degree of threat of extinction in their natural habitat. For this reason, felids have been included in conservation-related studies. This scenario has aroused increasing interest in the formation of somatic cell banks, which when efficiently implemented can be used in preservation strategies for the species. Nevertheless, one of the important steps in the formation of these banks is the understanding of the technical principles and variations involved in cryopreservation techniques, especially because cryopreservation increases the possibilities for Assisted Reproduction Technologies (ARTs) by making the use of biological materials independent of time and space. In wild felids, several species already have promising results in the formation of somatic cell banks, and studies aimed at better viability rates have been constantly proposed, as well as new species have been studied. In some species, aspects involved in successful cryopreservation are already well defined, and slow freezing associated with cryoprotectant solutions composed of intra- and extracellular substances is the most useful approach. The aim of this review was to present the main parameters involved in the elaboration of a somatic cell cryopreservation protocol and their effects, as well as to address the main results achieved for different wild felids. Doi.org/10.54680/fr23510110112.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"44 5","pages":"249-257"},"PeriodicalIF":1.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138458434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supplementation of sulfate polysaccharides in the seminal cooling medium of common curimata (Prochilodus brevis). 枸杞种子冷却液中添加硫酸多糖的研究。

IF 1 4区生物学

Cryo letters Pub Date : 2023-07-01 DOI: 10.54680/fr23410110512

Y. S. Sales, Jéssica SalesLobato, Carla Tatiana Nascimento Sousa Vieira, João Eudes Faria Cavalcante Filho, Yasmim Maia Ferreira, Marcos Luiz da Silva Apoliano, R. V. Nascimento, Silvio Alencar Căndido Sobrinho, José Ariévilo Gurgel Rodrigues, Carla Pamela Braga Guia, Fernanda Vitória Almeida Magalhães, C. Salmito‐Vanderley

{"title":"Supplementation of sulfate polysaccharides in the seminal cooling medium of common curimata (Prochilodus brevis).","authors":"Y. S. Sales, Jéssica SalesLobato, Carla Tatiana Nascimento Sousa Vieira, João Eudes Faria Cavalcante Filho, Yasmim Maia Ferreira, Marcos Luiz da Silva Apoliano, R. V. Nascimento, Silvio Alencar Căndido Sobrinho, José Ariévilo Gurgel Rodrigues, Carla Pamela Braga Guia, Fernanda Vitória Almeida Magalhães, C. Salmito‐Vanderley","doi":"10.54680/fr23410110512","DOIUrl":"https://doi.org/10.54680/fr23410110512","url":null,"abstract":"BACKGROUND\u0000The use of sulfated polysaccharides (PS) in seminal cooling is known to improve seminal quality.\u0000\u0000\u0000OBJECTIVE\u0000To evaluate the effect of different concentrations of PS, extracted from the macroalgae Gracilaria domigensis as a supplement to the seminal cooling medium of the reophilic fish Prochilodus brevis (common curimatã).\u0000\u0000\u0000MATERIALS AND METHODS\u0000Five semen pools were diluted in ACP-104 (treatment T1), in BTS® (T2) and in BTS® with different concentrations of PS (0.5 [T3]; 1.0 [T4] and 1.5 [T5]). The samples were cooled for different times (0, 6, 24, 48, 72, 96 and 120 h) and after each hour they were analyzed for: morphology, membrane integrity, DNA integrity and sperm kinetics.\u0000\u0000\u0000RESULTS\u0000There were no significant differences between the treatments containing different concentrations of sulfated polysaccharides. Regarding the different cooling times, it was possible to observe that after hour 96, there was a reduction in the parameters of sperm kinetics. For DNA integrity there was no significant difference in relation to the treatments nor in relation to the hours. For membrane integrity, a reduction was noted as of hour 96, but there was no influence of polysaccharides. For the sperm morphology, there was no statistical difference between the hours, however the BTS was better than the ACP-104.\u0000\u0000\u0000CONCLUSION\u0000It is concluded that the use of polysaccharides in seminal cooling has no negative effect on sperm parameters and proves that seminal cooling keeps the material viable for up to 72 hours. Doi: 10.54680/fr23410110512.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"2013 1","pages":"208-218"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86274886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryopreservation of zygotic embryos of Podophyllum hexandrum Royle, an endangered medicinal plant, by vitrification and v cryo-plate techniques. 玻璃化和冷冻板技术对濒危药用植物六脚木合子胚胎的低温保存。

IF 1 4区生物学

Cryo letters Pub Date : 2023-07-01 DOI: 10.54680/fr23410110712

Kiran Parasher, Shailika Sharma, Papiya Mukherjee, P. Qazi

{"title":"Cryopreservation of zygotic embryos of Podophyllum hexandrum Royle, an endangered medicinal plant, by vitrification and v cryo-plate techniques.","authors":"Kiran Parasher, Shailika Sharma, Papiya Mukherjee, P. Qazi","doi":"10.54680/fr23410110712","DOIUrl":"https://doi.org/10.54680/fr23410110712","url":null,"abstract":"BACKGROUND\u0000Podophyllum hexandrum is a highly endangered valuable medicinal plant of the Himalayas belonging to family Berberidaceae. This plant needs conservation efforts due to the over-exploitation and unscrupulous harvesting from the wild because of its ever-increasing demand.\u0000\u0000\u0000OBJECTIVE\u0000To establish a long-term cryopreservation method for Podophyllum hexandrum using two techniques: Vitrification and V Cryo-plate.\u0000\u0000\u0000MATERIALS AND METHODS\u0000Zygotic embryos were cryopreserved using vitrification and V cryo-plate by optimization of parameters including preculture time, loading time and PVS2 dehydration time. Recovery of zygotic embryos was performed on different regrowth media for plantlet formation.\u0000\u0000\u0000RESULTS\u0000With V cryo-plate, 90% regrowth was obtained as compared to 73.3% with vitrification. V Cryo-plate conditions were pre-culture of zygotic embryos in 0.3 M sucrose for 4 days, treatment in loading solution with 0.8 M sucrose for 20 min, dehydration in PVS2 for 50 min, LN exposure, unloading in 1.2 M sucrose for 20 min and transfer of zygotic embryos to regrowth medium for recovery. During recovery, the maximum number of shoots (4.2) and highest shoot length (5.1 cm) were observed on regrowth medium with 1.5 mg per liter BAP and 0.1 mg per liter IAA (R7).\u0000\u0000\u0000CONCLUSION\u0000Zygotic embryos of Podophyllum hexandrum were cryopreserved with 90% regrowth using a V cryoplate technique and plantlets were produced directly after cryopreservation. Doi: 10.54680/fr23410110712.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"4 1","pages":"219-228"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89835481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supplementation of sulfate polysaccharides in the seminal cooling medium of common curimata (Prochilodus brevis). 短柱原球茎种子冷却培养基中硫酸多糖的补充。

IF 1 4区生物学

Cryo letters Pub Date : 2023-07-01

Y S Sales, J S Lobato, C T Nascimento Sousa Vieira, J E Faria Cavalcante Filho, Y M Ferreira, M L da Silva Apoliano, R V do Nascimento, S A Candido Sobrinho, J A Gurgel Rodrigues, C P Braga Guia, F V Almeida Magalhaes, C S Salmito-Vanderley

{"title":"Supplementation of sulfate polysaccharides in the seminal cooling medium of common curimata (Prochilodus brevis).","authors":"Y S Sales, J S Lobato, C T Nascimento Sousa Vieira, J E Faria Cavalcante Filho, Y M Ferreira, M L da Silva Apoliano, R V do Nascimento, S A Candido Sobrinho, J A Gurgel Rodrigues, C P Braga Guia, F V Almeida Magalhaes, C S Salmito-Vanderley","doi":"","DOIUrl":"","url":null,"abstract":"Background: The use of sulfated polysaccharides (PS) in seminal cooling is known to improve seminal quality.Objective: To evaluate the effect of different concentrations of PS, extracted from the macroalgae Gracilaria domigensis as a supplement to the seminal cooling medium of the reophilic fish Prochilodus brevis (common curimatã).Materials and methods: Five semen pools were diluted in ACP-104 (treatment T1), in BTS® (T2) and in BTS® with different concentrations of PS (0.5 [T3]; 1.0 [T4] and 1.5 [T5]). The samples were cooled for different times (0, 6, 24, 48, 72, 96 and 120 h) and after each hour they were analyzed for: morphology, membrane integrity, DNA integrity and sperm kinetics.Results: There were no significant differences between the treatments containing different concentrations of sulfated polysaccharides. Regarding the different cooling times, it was possible to observe that after hour 96, there was a reduction in the parameters of sperm kinetics. For DNA integrity there was no significant difference in relation to the treatments nor in relation to the hours. For membrane integrity, a reduction was noted as of hour 96, but there was no influence of polysaccharides. For the sperm morphology, there was no statistical difference between the hours, however the BTS was better than the ACP-104.Conclusion: It is concluded that the use of polysaccharides in seminal cooling has no negative effect on sperm parameters and proves that seminal cooling keeps the material viable for up to 72 hours. Doi: 10.54680/fr23410110512.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"44 4","pages":"208-218"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50160984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0