In vitro and in vivo effect of oxytetracycline on sperm parameters in breeding rooster.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2023-09-01 DOI:10.54680/fr23510110412

Linda Mohammedi, Ahmed Messai, L. Touazi, M. Iguer-Ouada

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引用次数: 0

Abstract

BACKGROUND Some antimicrobials could adversely affect sperm quality during sperm cryopreservation and antibiotic treatment with subsequent effects on fertility outputs. To our knowledge, no similar studies have been conducted on breeding roosters, especially for oxytetracycline (OTC). OBJECTIVE To investigate both in vitro and in vivo impact of oxytetracycline on sperm parameters in breeding roosters. METHODS Sperm motility parameters were objectively analyzed using the CASA system including total motility (TM %), progressive motility (PM %), all sperm velocities, the sperm count, and cell viability during 9 days of in vivo treatment. In the in vitro investigation, the pooled sperm was diluted and divided into a control aliquot (diluted in 0.9% NaCl) and treated samples. Motility parameters were assessed after 0, 1, 2, 3, 4, 5, and 6 hours of storage at 37ºC. In the in vivo study, 1 g per L of OTC was administrated to five individuals for nine consecutive days. Fresh semen samples were analyzed at T0 (before treatment) and after 6 (T6) and 9 days (T9) of treatment. RESULTS OTC caused significant impairment of sperm quality in vivo. A drastic reduction in sperm concentration, viability, TM, PM, and all kinematic parameters was observed after 6 days of treatment. However, at day 9 sperm quality had improved to be nearly similar to T0. In vitro, OTC induced similar sperm impairment on all sperm motility parameters. CONCLUSION Oxytetracycline exhibited negative effects on rooster sperm both in vivo and in vitro and appears consequently not suitable in cryopreservation extenders. Doi.org/10.54680/fr23510110412.

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土霉素对种公鸡精子参数的体内外影响

背景：在精子冷冻保存和抗生素治疗过程中，一些抗菌药物可能会对精子质量产生不利影响，进而影响生育能力。据我们所知，目前还没有针对种公鸡，尤其是土霉素（OTC）进行过类似的研究。目的研究土霉素在体外和体内对种公鸡精子参数的影响。方法使用 CASA 系统客观分析精子活力参数，包括总活力（TM %）、渐进式活力（PM %）、所有精子速度、精子数量以及体内治疗 9 天期间的细胞活力。在体外研究中，将汇集的精子稀释并分成对照等分样品（用 0.9% 氯化钠稀释）和处理过的样品。在 37ºC 温度下保存 0、1、2、3、4、5 和 6 小时后，对精子的运动参数进行评估。在体内研究中，连续九天给五个人服用每升 1 克的 OTC。在 T0（治疗前）、治疗 6 天（T6）和 9 天（T9）后对新鲜精液样本进行分析。结果 OTC 严重损害了体内精子的质量。治疗 6 天后，观察到精子浓度、存活率、TM、PM 和所有运动参数急剧下降。然而，在第 9 天，精子质量有所改善，几乎与 T0 相似。在体外，OTC 对精子的所有运动参数都造成了类似的损害。结论土霉素对公鸡精子在体内和体外都有负面影响，因此不适合用于冷冻保存扩展剂。Doi.org/10.54680/fr23510110412.

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.