用医用纱布法吸管玻璃化细胞增强传热。

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2023-09-01

S Tao, B Liu

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引用次数: 0

摘要

背景:样品在液氮冷却过程中会发生膜沸腾，这限制了传热，降低了冷却速率。目的与方法:本研究提出了一种通过在法式吸管(FS)冷却时包裹一层医用纱布来消除膜沸腾的方法，以增强对流换热。采用数值模型研究了该方法的热力学机理。结果:基于传热和结晶方程的数值模拟表明，医用纱布包裹可抑制膜沸腾。实验验证表明，包裹FS能提高冷却速度，提高细胞存活率。结论:数值模拟和实验验证了医用纱布包裹FS能更好地保存细胞。Doi.org/10.54680/fr23510110312。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Enhanced heat transfer by medical gauze for cell vitrification with French straw.

Background: Film boiling occurs in the cooling process of samples with liquid nitrogen, which limits heat transfer and decreases the cooling rate.

Objective and methods: The study developed a method to enhance convective heat transfer by wrapping the French straw (FS) with a layer of medical gauze upon cooling to eliminate film boiling. A numerical model was used to study the thermodynamic mechanism in the method.

Results: Numerical simulation based on heat transfer and crystallization equations indicated that wrapping the FS with medical gauze could suppress film boiling. Experimental verification showed the increased cooling rate and better cell survival when wrapped FS was used.

Conclusion: Numerical simulation and experimental verification demonstrated the efficacy of wrapping FS with medical gauze for better cell cryopreservation. Doi.org/10.54680/fr23510110312.

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.