Cryo letters最新文献_第5页

Enhanced heat transfer by medical gauze for cell vitrification with French straw. 用医用纱布法吸管玻璃化细胞增强传热。

IF 1 4区生物学

Cryo letters Pub Date : 2023-09-01

S Tao, B Liu

引用次数: 0

Enhanced heat transfer by medical gauze for cell vitrification with French straw. 利用医用纱布增强热传导，实现法国秸秆细胞玻璃化。

IF 1 4区生物学

Cryo letters Pub Date : 2023-09-01 DOI: 10.54680/fr23510110312

Tao Song, Baolin Liu

引用次数: 0

In vitro and in vivo effect of oxytetracycline on sperm parameters in breeding rooster. 土霉素对种公鸡精子参数的体内外影响

IF 1 4区生物学

Cryo letters Pub Date : 2023-09-01 DOI: 10.54680/fr23510110412

Linda Mohammedi, Ahmed Messai, L. Touazi, M. Iguer-Ouada

{"title":"In vitro and in vivo effect of oxytetracycline on sperm parameters in breeding rooster.","authors":"Linda Mohammedi, Ahmed Messai, L. Touazi, M. Iguer-Ouada","doi":"10.54680/fr23510110412","DOIUrl":"https://doi.org/10.54680/fr23510110412","url":null,"abstract":"BACKGROUND Some antimicrobials could adversely affect sperm quality during sperm cryopreservation and antibiotic treatment with subsequent effects on fertility outputs. To our knowledge, no similar studies have been conducted on breeding roosters, especially for oxytetracycline (OTC). OBJECTIVE To investigate both in vitro and in vivo impact of oxytetracycline on sperm parameters in breeding roosters. METHODS Sperm motility parameters were objectively analyzed using the CASA system including total motility (TM %), progressive motility (PM %), all sperm velocities, the sperm count, and cell viability during 9 days of in vivo treatment. In the in vitro investigation, the pooled sperm was diluted and divided into a control aliquot (diluted in 0.9% NaCl) and treated samples. Motility parameters were assessed after 0, 1, 2, 3, 4, 5, and 6 hours of storage at 37ºC. In the in vivo study, 1 g per L of OTC was administrated to five individuals for nine consecutive days. Fresh semen samples were analyzed at T0 (before treatment) and after 6 (T6) and 9 days (T9) of treatment. RESULTS OTC caused significant impairment of sperm quality in vivo. A drastic reduction in sperm concentration, viability, TM, PM, and all kinematic parameters was observed after 6 days of treatment. However, at day 9 sperm quality had improved to be nearly similar to T0. In vitro, OTC induced similar sperm impairment on all sperm motility parameters. CONCLUSION Oxytetracycline exhibited negative effects on rooster sperm both in vivo and in vitro and appears consequently not suitable in cryopreservation extenders. Doi.org/10.54680/fr23510110412.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"100 1","pages":"291-298"},"PeriodicalIF":1.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139346952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PERSPECTIVE: Potential and reality of cryopreserving somatic cells of wild felids for conservation. 展望:野生猫科动物体细胞低温保存的潜力与现实。

IF 1 4区生物学

Cryo letters Pub Date : 2023-09-01

L L Vieira Rodrigues, A F Pereira

{"title":"PERSPECTIVE: Potential and reality of cryopreserving somatic cells of wild felids for conservation.","authors":"L L Vieira Rodrigues, A F Pereira","doi":"","DOIUrl":"","url":null,"abstract":"The loss of biodiversity caused by anthropogenic actions is also a reality for the members of the Felidae family. Except for the domestic cat, all felid species have some degree of threat of extinction in their natural habitat. For this reason, felids have been included in conservation-related studies. This scenario has aroused increasing interest in the formation of somatic cell banks, which when efficiently implemented can be used in preservation strategies for the species. Nevertheless, one of the important steps in the formation of these banks is the understanding of the technical principles and variations involved in cryopreservation techniques, especially because cryopreservation increases the possibilities for Assisted Reproduction Technologies (ARTs) by making the use of biological materials independent of time and space. In wild felids, several species already have promising results in the formation of somatic cell banks, and studies aimed at better viability rates have been constantly proposed, as well as new species have been studied. In some species, aspects involved in successful cryopreservation are already well defined, and slow freezing associated with cryoprotectant solutions composed of intra- and extracellular substances is the most useful approach. The aim of this review was to present the main parameters involved in the elaboration of a somatic cell cryopreservation protocol and their effects, as well as to address the main results achieved for different wild felids. Doi.org/10.54680/fr23510110112.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"44 5","pages":"249-257"},"PeriodicalIF":1.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138458434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supplementation of sulfate polysaccharides in the seminal cooling medium of common curimata (Prochilodus brevis). 枸杞种子冷却液中添加硫酸多糖的研究。

IF 1 4区生物学

Cryo letters Pub Date : 2023-07-01 DOI: 10.54680/fr23410110512

Y. S. Sales, Jéssica SalesLobato, Carla Tatiana Nascimento Sousa Vieira, João Eudes Faria Cavalcante Filho, Yasmim Maia Ferreira, Marcos Luiz da Silva Apoliano, R. V. Nascimento, Silvio Alencar Căndido Sobrinho, José Ariévilo Gurgel Rodrigues, Carla Pamela Braga Guia, Fernanda Vitória Almeida Magalhães, C. Salmito‐Vanderley

{"title":"Supplementation of sulfate polysaccharides in the seminal cooling medium of common curimata (Prochilodus brevis).","authors":"Y. S. Sales, Jéssica SalesLobato, Carla Tatiana Nascimento Sousa Vieira, João Eudes Faria Cavalcante Filho, Yasmim Maia Ferreira, Marcos Luiz da Silva Apoliano, R. V. Nascimento, Silvio Alencar Căndido Sobrinho, José Ariévilo Gurgel Rodrigues, Carla Pamela Braga Guia, Fernanda Vitória Almeida Magalhães, C. Salmito‐Vanderley","doi":"10.54680/fr23410110512","DOIUrl":"https://doi.org/10.54680/fr23410110512","url":null,"abstract":"BACKGROUND\u0000The use of sulfated polysaccharides (PS) in seminal cooling is known to improve seminal quality.\u0000\u0000\u0000OBJECTIVE\u0000To evaluate the effect of different concentrations of PS, extracted from the macroalgae Gracilaria domigensis as a supplement to the seminal cooling medium of the reophilic fish Prochilodus brevis (common curimatã).\u0000\u0000\u0000MATERIALS AND METHODS\u0000Five semen pools were diluted in ACP-104 (treatment T1), in BTS® (T2) and in BTS® with different concentrations of PS (0.5 [T3]; 1.0 [T4] and 1.5 [T5]). The samples were cooled for different times (0, 6, 24, 48, 72, 96 and 120 h) and after each hour they were analyzed for: morphology, membrane integrity, DNA integrity and sperm kinetics.\u0000\u0000\u0000RESULTS\u0000There were no significant differences between the treatments containing different concentrations of sulfated polysaccharides. Regarding the different cooling times, it was possible to observe that after hour 96, there was a reduction in the parameters of sperm kinetics. For DNA integrity there was no significant difference in relation to the treatments nor in relation to the hours. For membrane integrity, a reduction was noted as of hour 96, but there was no influence of polysaccharides. For the sperm morphology, there was no statistical difference between the hours, however the BTS was better than the ACP-104.\u0000\u0000\u0000CONCLUSION\u0000It is concluded that the use of polysaccharides in seminal cooling has no negative effect on sperm parameters and proves that seminal cooling keeps the material viable for up to 72 hours. Doi: 10.54680/fr23410110512.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"2013 1","pages":"208-218"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86274886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryopreservation of zygotic embryos of Podophyllum hexandrum Royle, an endangered medicinal plant, by vitrification and v cryo-plate techniques. 玻璃化和冷冻板技术对濒危药用植物六脚木合子胚胎的低温保存。

IF 1 4区生物学

Cryo letters Pub Date : 2023-07-01 DOI: 10.54680/fr23410110712

Kiran Parasher, Shailika Sharma, Papiya Mukherjee, P. Qazi

{"title":"Cryopreservation of zygotic embryos of Podophyllum hexandrum Royle, an endangered medicinal plant, by vitrification and v cryo-plate techniques.","authors":"Kiran Parasher, Shailika Sharma, Papiya Mukherjee, P. Qazi","doi":"10.54680/fr23410110712","DOIUrl":"https://doi.org/10.54680/fr23410110712","url":null,"abstract":"BACKGROUND\u0000Podophyllum hexandrum is a highly endangered valuable medicinal plant of the Himalayas belonging to family Berberidaceae. This plant needs conservation efforts due to the over-exploitation and unscrupulous harvesting from the wild because of its ever-increasing demand.\u0000\u0000\u0000OBJECTIVE\u0000To establish a long-term cryopreservation method for Podophyllum hexandrum using two techniques: Vitrification and V Cryo-plate.\u0000\u0000\u0000MATERIALS AND METHODS\u0000Zygotic embryos were cryopreserved using vitrification and V cryo-plate by optimization of parameters including preculture time, loading time and PVS2 dehydration time. Recovery of zygotic embryos was performed on different regrowth media for plantlet formation.\u0000\u0000\u0000RESULTS\u0000With V cryo-plate, 90% regrowth was obtained as compared to 73.3% with vitrification. V Cryo-plate conditions were pre-culture of zygotic embryos in 0.3 M sucrose for 4 days, treatment in loading solution with 0.8 M sucrose for 20 min, dehydration in PVS2 for 50 min, LN exposure, unloading in 1.2 M sucrose for 20 min and transfer of zygotic embryos to regrowth medium for recovery. During recovery, the maximum number of shoots (4.2) and highest shoot length (5.1 cm) were observed on regrowth medium with 1.5 mg per liter BAP and 0.1 mg per liter IAA (R7).\u0000\u0000\u0000CONCLUSION\u0000Zygotic embryos of Podophyllum hexandrum were cryopreserved with 90% regrowth using a V cryoplate technique and plantlets were produced directly after cryopreservation. Doi: 10.54680/fr23410110712.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"4 1","pages":"219-228"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89835481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supplementation of sulfate polysaccharides in the seminal cooling medium of common curimata (Prochilodus brevis). 短柱原球茎种子冷却培养基中硫酸多糖的补充。

IF 1 4区生物学

Cryo letters Pub Date : 2023-07-01

Y S Sales, J S Lobato, C T Nascimento Sousa Vieira, J E Faria Cavalcante Filho, Y M Ferreira, M L da Silva Apoliano, R V do Nascimento, S A Candido Sobrinho, J A Gurgel Rodrigues, C P Braga Guia, F V Almeida Magalhaes, C S Salmito-Vanderley

{"title":"Supplementation of sulfate polysaccharides in the seminal cooling medium of common curimata (Prochilodus brevis).","authors":"Y S Sales, J S Lobato, C T Nascimento Sousa Vieira, J E Faria Cavalcante Filho, Y M Ferreira, M L da Silva Apoliano, R V do Nascimento, S A Candido Sobrinho, J A Gurgel Rodrigues, C P Braga Guia, F V Almeida Magalhaes, C S Salmito-Vanderley","doi":"","DOIUrl":"","url":null,"abstract":"Background: The use of sulfated polysaccharides (PS) in seminal cooling is known to improve seminal quality.Objective: To evaluate the effect of different concentrations of PS, extracted from the macroalgae Gracilaria domigensis as a supplement to the seminal cooling medium of the reophilic fish Prochilodus brevis (common curimatã).Materials and methods: Five semen pools were diluted in ACP-104 (treatment T1), in BTS® (T2) and in BTS® with different concentrations of PS (0.5 [T3]; 1.0 [T4] and 1.5 [T5]). The samples were cooled for different times (0, 6, 24, 48, 72, 96 and 120 h) and after each hour they were analyzed for: morphology, membrane integrity, DNA integrity and sperm kinetics.Results: There were no significant differences between the treatments containing different concentrations of sulfated polysaccharides. Regarding the different cooling times, it was possible to observe that after hour 96, there was a reduction in the parameters of sperm kinetics. For DNA integrity there was no significant difference in relation to the treatments nor in relation to the hours. For membrane integrity, a reduction was noted as of hour 96, but there was no influence of polysaccharides. For the sperm morphology, there was no statistical difference between the hours, however the BTS was better than the ACP-104.Conclusion: It is concluded that the use of polysaccharides in seminal cooling has no negative effect on sperm parameters and proves that seminal cooling keeps the material viable for up to 72 hours. Doi: 10.54680/fr23410110512.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"44 4","pages":"208-218"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50160984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strip pulled straw: cost effective alternative cryodevice for the vitrification of oocytes. 条状吸管:成本效益高的卵母细胞玻璃化冷冻装置。

IF 1 4区生物学

Cryo letters Pub Date : 2023-07-01 DOI: 10.54680/fr23410110212

Khursheed Ahmad Sofi, Beenish Qureshi

{"title":"Strip pulled straw: cost effective alternative cryodevice for the vitrification of oocytes.","authors":"Khursheed Ahmad Sofi, Beenish Qureshi","doi":"10.54680/fr23410110212","DOIUrl":"https://doi.org/10.54680/fr23410110212","url":null,"abstract":"BACKGROUND\u0000Increased cooling and warming rates by using a suitable cryodevice allows the use of lower cryoprotectant concentration and reduces cryoinjuries as a result of the rapid passage through the 'dangerous' temperature zone.\u0000\u0000\u0000OBJECTIVE\u0000To evaluate the effectiveness of newly customized strip pulled straw (SPS) with respect to post warming quality, viability, and in vitro maturation for immature oocytes post-vitrification of.\u0000\u0000\u0000MATERIALS AND METHODS\u0000SPS was prepared using conventional French mini straw to combine the merits of OPS and the Cryotop system. Immature sheep oocytes were treated in 15% EG + 15% DMSO, loaded on SPS and plunged into liquid nitrogen (LN). Post thaw quality, viability, and maturation rates of oocytes were determined after 1 week storage in LN.\u0000\u0000\u0000RESULTS\u0000SPS achieved a post-thaw morphological survival of 90.9% with 9.0% morphological defects, 96.4% viability and 51% in vitro maturation. In comparison to OPS, SPS had higher post thaw survival (86.5% vs 67.9%) and maturation rate (57.7% vs 50.5%) with lower morphological defects (13.5% vs 32.1%). Cumulus cell loss was the highest among morphological abnormalities of post warm oocytes in SPS (40.9%) and OPS (44.1%). The data showed acceptable post thaw survival, viability and in vitro maturation rate of immature ovine oocytes using SPS as compared to traditional OPS.\u0000\u0000\u0000CONCLUSION\u0000SPS can be used as a cost effective alternative device for oocyte vitrification. Doi: 10.54680/fr23410110212.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"31 1","pages":"229-233"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82174774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Freezing of equine semen is influenced by exposure time and concentration of the cryoprotectant glycerol. 马精液的冷冻受暴露时间和冷冻保护剂甘油浓度的影响。

IF 1 4区生物学

Cryo letters Pub Date : 2023-07-01

P L Fracaro, C D Corcini, N L Souza Gatti, J S Filho, I B Acosta, J S Filho, F P Hartwig, B D Rosa Curcio, A S Varela Junior

{"title":"Freezing of equine semen is influenced by exposure time and concentration of the cryoprotectant glycerol.","authors":"P L Fracaro, C D Corcini, N L Souza Gatti, J S Filho, I B Acosta, J S Filho, F P Hartwig, B D Rosa Curcio, A S Varela Junior","doi":"","DOIUrl":"","url":null,"abstract":"Background: Glycerol is a cryoprotectant widely used in the freezing of mammalian semen, but no study has demonstrated its optimum concentration and the appropriate exposure time for equine species.Objective: To demonstrate that the exposure time (15, 30, 45, 60, 75 and 90 min) versus concentration (2, 3, 4 and 5%) of the cryoprotectant glycerol influences the freezing success of equine semen.Materials and methods: The ejaculate of 12 stallions were frozen in different glycerol concentrations following different exposure times. The thawed sperm was evaluated for kinetic parameters using a Computer Assisted Semen Analysis (CASA) system and cell feature parameters were assessed by flow cytometry.Results: Considering the total and progressive motility of the spermatozoa, we concluded that protocols using 5% glycerol for 15 and 30 min exposure, 4% glycerol for 45 min exposure and 3% glycerol for 90 min exposure generated the best results.Conclusion: We suggest the use of any of these protocols for a better cryopreservation of equine semen. Doi: 10.54680/fr23410110412.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"44 4","pages":"234-239"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50160981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strip pulled straw: cost effective alternative cryodevice for the vitrification of oocytes. 条状吸管：用于卵母细胞玻璃化冷冻的成本效益高的替代冷冻装置。

IF 1 4区生物学

Cryo letters Pub Date : 2023-07-01

K A Sofi, B Qureshi

{"title":"Strip pulled straw: cost effective alternative cryodevice for the vitrification of oocytes.","authors":"K A Sofi, B Qureshi","doi":"","DOIUrl":"","url":null,"abstract":"Background: Increased cooling and warming rates by using a suitable cryodevice allows the use of lower cryoprotectant concentration and reduces cryoinjuries as a result of the rapid passage through the 'dangerous' temperature zone.Objective: To evaluate the effectiveness of newly customized strip pulled straw (SPS) with respect to post warming quality, viability, and in vitro maturation for immature oocytes post-vitrification of.Materials and methods: SPS was prepared using conventional French mini straw to combine the merits of OPS and the Cryotop system. Immature sheep oocytes were treated in 15% EG + 15% DMSO, loaded on SPS and plunged into liquid nitrogen (LN). Post thaw quality, viability, and maturation rates of oocytes were determined after 1 week storage in LN.Results: SPS achieved a post-thaw morphological survival of 90.9% with 9.0% morphological defects, 96.4% viability and 51% in vitro maturation. In comparison to OPS, SPS had higher post thaw survival (86.5% vs 67.9%) and maturation rate (57.7% vs 50.5%) with lower morphological defects (13.5% vs 32.1%). Cumulus cell loss was the highest among morphological abnormalities of post warm oocytes in SPS (40.9%) and OPS (44.1%). The data showed acceptable post thaw survival, viability and in vitro maturation rate of immature ovine oocytes using SPS as compared to traditional OPS.Conclusion: SPS can be used as a cost effective alternative device for oocyte vitrification. Doi: 10.54680/fr23410110212.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"44 4","pages":"229-233"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50160983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0