IGF-1 stabilizes goat sperm mitochondrial transmembrane potential and reduces dna fragmentation.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2023-11-01

R Ranjan, K Sharma, M Kumar, D K Swain, S P Singh, S D Kharche, M K Singh, M S Chauhan

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引用次数: 0

Abstract

Background: Antioxidant present in sperm cells protects them from oxidative damage. However, sperm are more susceptible to peroxidative damages due to the loss of these enzymes during cryopreservation and their survival and fertility may be compromised. Insulin like growth factor-1 (IGF-1) has an antioxidant effect and could maintain sperm motility.

Objective: To improve seminal parameters, mitochondrial membrane potential (MMP), oxidative status and DNA integrity of buck semen after freeze-thawing by fortification of goat semen diluent with various concentrations of IGF-1.

Materials and methods: Fifty ejaculates were collected and were extended with tris- citric acid- fructose diluent with 10% egg yolk and 6% glycerol with sperm concentrations of 1×10⁸ mL^-1. Post-cryopreserved sperm were assessed for motility and a range of other functional parameters.

Results: In post-thaw semen sperm motility, live sperm count, acrosome integrity, hypo-osmotic swelling positive spermatozoa, malondialdehyde (MDA), protein carbonyl content (PCC), TUNEL positive sperm differed significantly (P<0.05) with the various concentrations of IGF-1 used. Sperm functional parameters post-thawing were significantly (P<0.05) better in 250 ng/mL IGF-1. IGF-1 protects against lipid peroxidation by lowering MDA and PCC production, thus reducing the harmful effect of reactive oxygen species. The kidding percentage using the artificial insemination technique was significantly higher ( i.e., 40%) in the group supplemented with 250 ng/mL of IGF-1 than in the non-supplemented group (i.e., 30%).

Conclusion: IGF-1 may be used to improve post-thaw semen quality and fertility as measured by actual kidding rate. Doi.org/10.54680/fr23610110312.

本刊更多论文

IGF-1 可稳定山羊精子线粒体跨膜电位并减少 DNA 断裂。

背景：精子细胞中的抗氧化剂可保护精子免受氧化损伤。然而，在冷冻保存过程中，由于这些酶的损失，精子更容易受到过氧化损伤，其存活率和生育能力可能会受到影响。胰岛素样生长因子-1（IGF-1）具有抗氧化作用，可保持精子活力：通过在山羊精液稀释液中添加不同浓度的 IGF-1 来改善冻融后雄鹿精液的精液参数、线粒体膜电位（MMP）、氧化状态和 DNA 完整性：收集 50 个射精，用含 10%蛋黄和 6%甘油的三柠檬酸果糖稀释液稀释，精子浓度为 1×108 mL-1。对冷冻保存后的精子进行运动能力和一系列其他功能参数的评估：结果：解冻后精液中的精子运动能力、活精子数量、顶体完整性、低渗肿胀阳性精子、丙二醛（MDA）、蛋白质羰基含量（PCC）、TUNEL阳性精子均有显著差异（PC结论：IGF-1可用于提高精子的运动能力，并能提高精子的活力：IGF-1可用于改善解冻后精液的质量，并通过实际受精率来衡量受精率。Doi.org/10.54680/fr23610110312.

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.