Determination of the cryoprotective effect of n-methylacetamide in rabbit semen.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2023-11-01

S A Akarsu, I H Gungor, T C Acisu, A Cakir, E Guler, R H Koca, I Yilmaz, M Sonmez, S Gur, G Turk, S O Kaya, A Yuce

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引用次数: 0

Abstract

Background: Amides are low molecular weight cryoprotectants. N-methylacetamide (MA) is one of the cryoprotectant agents in this group.

Objective: To investigate the cryoprotective effect of MA in rabbit semen.

Materials and methods: For this purpose, six ejaculates from six New Zealand rabbits were collected and pooled using an artificial vagina. Pooled semen was divided into four equal parts and diluted with TCG+ egg yolk. CPA was added to form the following groups: Control with 6% DMSO; Group 1 with 1% MA; Group 2 with 2% MA; and Group 3 with 3% MA. After the addition of CPA, the semen eqilibration procedure was started. Sperm were then drawn into 0.25 mL straws, frozen by automatic semen freezing and stored in a liquid nitrogen container. Pipettes were thawed after 24 h and analyses were performed.

Results: Total, progressive and rapid motility values of the Control group were higher than those of the MA groups (p<0.05). However, there was no statistical difference between the Control and Group 2 in terms of these parameters. While there was no statistical difference between the groups in terms of acrosome damage and mitochondrial membrane potential, the best results were observed in Control, Group 2, Group 1 and Group 3, respectively. When we compared all groups, no difference was found in terms of MDA, CAT and GSH-Px. There was a statistical difference between Group 3 and the Control in terms of GSH level (p<0.05).

Conclusion: DMSO appeared to be more useful for the cryopreservation of rabbit semen compared to MA. Doi.org/10.54680/fr23610110812.

本刊更多论文

测定正甲基乙酰胺在兔精液中的冷冻保护作用。

背景：酰胺是低分子量冷冻保护剂。N-甲基乙酰胺（MA）是这类低温保护剂中的一种：目的：研究 MA 在兔精液中的冷冻保护作用：为此，使用人工阴道收集并汇集了六只新西兰兔的六次射精。将精液分成四等份，用 TCG+ 蛋黄稀释。加入 CPA，形成以下组别：对照组：6% DMSO；第 1 组：1% MA；第 2 组：2% MA；第 3 组：3% MA。添加 CPA 后，开始精液校准程序。然后将精液抽取到 0.25 mL 的吸管中，用精液自动冷冻装置冷冻并储存在液氮容器中。24 小时后解冻吸管并进行分析：结果：对照组的总活力值、渐进活力值和快速活力值均高于 MA 组（p）：结论：与 MA 相比，DMSO 似乎更适合用于兔精液的冷冻保存。Doi.org/10.54680/fr23610110812.

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.