The effect of different concentrations of laminarin on the quality of cryopreserved ram semen.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2024-01-01

N Zangishhi, H Hajarian, H Karamishabankareh, L Soltani

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引用次数: 0

Abstract

Background: Increasingly, sheep breeders are using artificial insemination to produce lambs, so finding methods that preserve ram sperm can be useful.

Objective: To determine the protective effects of different concentrations of laminarin on ram sperm motility, viability, abnormalities, membrane, and DNA integrity, superoxide dismutase enzyme (SOD) activity, and malondialdehyde (MDA) production after freeze-thawing.

Materials and methods: The ejaculates of four rams were collected and stored at 35 degree C. Semen samples were diluted with a tris-base extender containing 100, 200, 400, and 800 ug/mL of laminarin and a control extender containing no laminarin, then frozen in liquid nitrogen after 4 h in the refrigerator.

Results: In the treatment of frozen-thawed spermatozoa with 800 ug/mL laminarin, motility, viability, membrane integrity, and DNA integrity were significantly higher than in the control. In spermatozoa that were exposed to 800 ug/mL laminarin after thawing, MDA production was significantly lower than in the control group. The percentage of abnormal spermatozoa in 800 ug/mL laminarin was significantly lower than that in the control.

Conclusion: The addition of 800 ug/mL laminarin to the freezing extender increases motility, viability, SOD activity, and plasma membrane integrity, while reducing abnormality and MDA production in freeze-thawed ram semen. https://doi.org/10.54680/fr24110110812.

本刊更多论文

不同浓度的层粘蛋白对冷冻保存的公羊精液质量的影响。

背景：越来越多的绵羊饲养者使用人工授精来生产羔羊，因此找到保护公羊精子的方法非常有用：材料与方法：收集四只公羊的精子，并将其保存在 35℃的环境中：用含100、200、400和800微克/毫升层皮素的三碱基扩增剂和不含层皮素的对照扩增剂稀释精液样本，然后在冰箱中冷冻4小时后用液氮冷冻：结果：用 800 微克/毫升的层黏蛋白酶处理冷冻解冻的精子，其运动能力、存活率、膜完整性和 DNA 完整性均显著高于对照组。解冻后接触 800 微克/毫升层粘蛋白的精子中，MDA 的产生量明显低于对照组。畸形精子在800微克/毫升层析蛋白中的比例明显低于对照组：结论：在冷冻扩展剂中添加 800 微克/毫升的层黏蛋白可提高冻融公羊精液的活力、存活率、SOD 活性和质膜完整性，同时减少畸形精子和 MDA 的产生。https://doi.org/10.54680/fr24110110812。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.