Role of oleic acid and trehalose on frozen-thawed ram semen.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2023-11-01

L Soltani

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引用次数: 0

Abstract

Background: When sperm are cryopreserved, reactive oxygen species (ROS) are formed that are detrimental to the sperm.

Objective: To evaluate the effects of oleic acid and trehalose added to ram semen extender on sperm parameters, lipid peroxidation (MDA), and superoxide dismutase (SOD) enzyme levels of spermatozoa following the freeze/thawing processes.

Materials and methods: Ejaculates were collected from four rams and pooled at 35 degree C. Pooled ejaculates were diluted with oleic acid at 0 mM and trehalose at 0 mM (O0 T0) as the control. The Tris-based extender was supplemented with either 0.5 (O0.5) or 1 (O1) mM of oleic acid or 25 (T25) or 50 (T50) mM of trehalose alone, and in combination [0.5 mM oleic acid + 25 mM trehalose (O0.5 T25), 0.5 mM oleic acid + 50 mM trehalose (O0.5 T50), 1 mM oleic acid + 25 mM trehalose (O1 T25) and 1 mM oleic acid + 50 mM trehalose (O1 T50)]. The semen was frozen by the traditional liquid nitrogen vapour method and stored at -196C in the liquid nitrogen tank.

Results: Semen extender containing O1T25 significantly improved the total motility, when compared with other treatment groups (P<0.05), except for O1 T50. O1 T50 had a higher viability rate than any other treatment. The addition of O1 T25 and O1 T50 increased DNA and membrane integrity of spermatozoa post-thawing compared to other treatments (P<0.05). The level of MDA was significantly (P<0.05) lower in extenders supplemented with O1, O0.5 T25, O0.5 T50, O1 T25 and O1T50 compared to the other treatment groups. In addition, SOD levels were higher in groups treated with O1 T25 and O1 T50 than the other treatment groups (P<0.05).

Conclusion: The addition of a combination of oleic acid and trehalose concentrations to Tris-based extender improved the quality of ram semen post-thawing. Doi.org/10.54680/fr23610110712.

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油酸和三卤糖对冷冻解冻公羊精液的作用

背景：冷冻保存精子时会形成对精子有害的活性氧（ROS）：评估在公羊精液扩展液中添加油酸和三卤糖对冷冻/解冻过程中精子参数、脂质过氧化（MDA）和超氧化物歧化酶（SOD）水平的影响：用 0 mM 的油酸和 0 mM 的树胶糖（O0 T0）稀释集中的精子作为对照。在基于 Tris 的扩展剂中单独添加 0.5（O0.5）或 1（O1）毫摩尔的油酸或 25（T25）或 50（T50）毫摩尔的妥尔糖，或同时添加[0.5（O0.5）毫摩尔油酸+50（T50）毫摩尔妥尔糖]。5 mM 油酸 + 25 mM 曲卤糖 (O0.5 T25)、0.5 mM 油酸 + 50 mM 曲卤糖 (O0.5 T50)、1 mM 油酸 + 25 mM 曲卤糖 (O1 T25) 和 1 mM 油酸 + 50 mM 曲卤糖 (O1 T50)]。精液采用传统的液氮蒸气法冷冻，并储存在零下 196 摄氏度的液氮罐中：结果：与其他处理组（PC）相比，含有 O1T25 的精液延长剂明显提高了精子的总活力：在基于三羟甲基氨基甲烷的精液延长剂中添加油酸和三卤糖的组合可提高公羊精液解冻后的质量。Doi.org/10.54680/fr23610110712.

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.