Cryobiology最新文献

{"title":"An efficient and convenient method for the preservation of germplasm resources: Cryopreservation of mature testis of large yellow croaker (Larimichthys crocea)","authors":"Feiyan Li ,&nbsp;Li Zhou ,&nbsp;Kunhuang Han ,&nbsp;Qi Wei ,&nbsp;Meixia Chen ,&nbsp;Zhaohan Sun ,&nbsp;Jia Chen ,&nbsp;Xiaoquan Jiang","doi":"10.1016/j.cryobiol.2025.105293","DOIUrl":"10.1016/j.cryobiol.2025.105293","url":null,"abstract":"<div><div>Large yellow croaker (<em>Larimichthys crocea</em>) is one of the most important marine economic fish. However, due to overfishing and long-term artificial breeding, the germplasm has degraded in recent years, and the wild germplasm has been listed as critically endangered. It is urgent to protect the germplasm resources of <em>Larimichthys crocea</em>. Cryopreservation may be an alternative way, however, the toxicity of cryoprotectants and the complicated preservation procedure that uses a programmable freezer has greatly increased the difficulty of cryopreservation. Therefore, in this study, we aimed to explore an efficient, convenient, inexpensive and low toxicity method for cryopreservation of mature testes in this species. The cryoprotectants of dimethyl sulfoxide (Me₂SO), ethylene glycol (EG), propylene glycol (PG) were selected to cryopreserve the mature testes with liquid nitrogen in simple style. Finally, the testes were cut into blocks (1.0 cm³) with cryoprotectants of 20 % PG and 15 % Me₂SO, placed at a position 10 cm above the liquid nitrogen for 10 min, then plunged into liquid nitrogen, resulting in high survival rate of 64.17 % at 3 days post cryopreservation. And the recovery rate of spermatogonia was more than 70 %. Subsequently, the cells viability were further detected by qRT-PCR, <em>in situ</em> hybridization (ISH) and germ cell transplantation. These results suggested that the spermatogonia from cryopreserved testes had high viability, which could migrate and colonized after transplantation. The present study first established cryopreservation protocols of mature testis in <em>Larimichthys crocea</em>, which will serve as a promising tool to protect Sciaenidae and other fish germplasm resources.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105293"},"PeriodicalIF":2.1,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144864717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of years of storage (1, 5, and 7 years) on bovine sperm ultrastructure, oxidative status, and fertilizing capacity","authors":"Chang-Guo Min ,&nbsp;Si-Min Wang ,&nbsp;Zhi-Le Fan ,&nbsp;Kai-Yan Zhang ,&nbsp;Sheng-Kui Hou ,&nbsp;Xin-Hai Wang ,&nbsp;Xin Ma ,&nbsp;Jun Wang ,&nbsp;Jing Zhao ,&nbsp;Yi Fang ,&nbsp;Hong-Yu Liu ,&nbsp;He Ding ,&nbsp;Jing Guo ,&nbsp;Wen-Fa Lu","doi":"10.1016/j.cryobiol.2025.105291","DOIUrl":"10.1016/j.cryobiol.2025.105291","url":null,"abstract":"<div><div>The cryopreservation technique for sperm is a widely recognized approach utilized in artificial insemination. Previous studies have demonstrated that sperm quality is influenced by ice crystal formation and oxidative stress during the freezing process. Moreover, the time of cryostorage also has an impact on sperm quality. Nevertheless, there is a scarcity of comprehensive research concerning the years of storage on bovine sperm. In this study, bovine sperm samples cryopreserved for 1, 5, and 7 years were analyzed for motility, functionality membrane integrity, oxidative stress markers, apoptosis, DNA fragmentation, and fertilizing ability. Post-thaw assessments revealed a significant decline in antioxidant capacity and an increase in lipid peroxidation and apoptosis in the 7-year group compared to the 1-year group (<em>P</em> &lt; 0.05). Despite relatively stable sperm membrane and acrosome integrity, DNA damage and oxidative stress were elevated over time. Notably, while cleavage rates remained unchanged, blastocyst development was significantly reduced after 7 years of storage (<em>P</em> &lt; 0.05). These findings suggest that prolonged cryopreservation negatively affects sperm function and embryo development, emphasizing the need to consider storage duration in reproductive applications.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105291"},"PeriodicalIF":2.1,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144831439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combination of trehalose and inulin efficacy as lyoprotectant for the preservation of human monocytic cell line","authors":"Alina Meiss,&nbsp;Gretel Chometon,&nbsp;Jonas Greinwald,&nbsp;Luis Gautzsch,&nbsp;Kilian Hennes","doi":"10.1016/j.cryobiol.2025.105287","DOIUrl":"10.1016/j.cryobiol.2025.105287","url":null,"abstract":"<div><div>Traditional cell cryopreservation is expensive and logistically complex, and it necessitates a constant cold chain [44,57]. Lyophilization presents an alluring substitute, so long as the right lyoprotectants are applied to preserve cell integrity [23,40,44,52]. This study examined whether the human THP-1 mononuclear cell line's viability and function are maintained during the lyophilization process when trehalose and inulin are combined as a lyoprotectant. Cell survival, membrane integrity, metabolic activity, and functional responsiveness (differentiation and cytokine secretion) were assessed after THP-1 cells were processed in various lyophilization conditions (trehalose alone versus trehalose in combination with inulin). The reference cells were cryopreserved cells. Following rehydration, the vitality of cells lyophilized in a mixture containing trehalose and inulin was comparable to that of cryopreserved cells. After a recovery period in culture, the cell membranes regained their integrity, despite being temporarily permeable right after rehydration. Additionally, the inclusion of inulin helped to reduce the residual moisture content and increase the stability of the lyophilizates, while also preserving metabolic activities and the ability to differentiate (including IL-1β release after stimulus). Trehalose and inulin work well together as a lyoprotectant, lowering the stress that THP-1 cells experience during the lyophilization process. Therefore, this approach may be a practical and affordable substitute for traditional cryopreservation, maintaining the cells' stability and activity even after extended storage.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105287"},"PeriodicalIF":2.1,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144809658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive proteomic, structural and functional analysis of cryoprotectant effects on marine mussel oocytes","authors":"Sofía Blanco ,&nbsp;Sara Campos ,&nbsp;Patricia Reboreda ,&nbsp;Estefanía Paredes ,&nbsp;Angel P. Diz","doi":"10.1016/j.cryobiol.2025.105288","DOIUrl":"10.1016/j.cryobiol.2025.105288","url":null,"abstract":"<div><div>Cryopreservation is a valuable tool for preserving marine genetic diversity and supporting selective breeding in aquaculture. The blue mussel (<em>Mytilus galloprovincialis</em>) is an important species for the aquaculture industry and a useful model for studying cryopreservation in marine invertebrates. Declining mussel seed availability over the past decade, combined with the growing demand for year-round hatchery production, has increased interest in developing effective cryopreservation methods for gametes and embryos. However, cryopreservation remains challenging, particularly for marine invertebrate oocytes, as it requires balancing cryoprotectant cytotoxicity with sufficient cell protection during freezing and thawing. Moreover, the molecular, structural, and functional changes associated with oocyte cryopreservation in invertebrates are still poorly understood. This study examines the effects of two commonly used cryoprotectants, dimethyl sulfoxide (DMSO) and ethylene glycol (EG), on <em>M. galloprovincialis</em> oocytes under a monitored slow freezing (MSF) protocol. Using an integrative analytical approach combining proteomics, functional assessments, and structural analyses, we provide a comprehensive understanding of cryoprotectant-induced changes. Our findings reveal that cryoprotectants cause significant proteomic alterations, with DMSO having more pronounced effects. These alterations are associated with increased oxidative stress, an insufficient antioxidant response, and disruptions in meiosis restart mechanisms, ultimately delaying larval development. Cryoprotectant-induced oxidative stress may also weaken the oocyte membrane, leading to rupture during the MSF protocol and subsequent fertilisation failure post-thawing. These results provide evidence that EG is less cytotoxic than DMSO and suggest that supplementing cryoprotectants with antioxidants and membrane stabilizers could enhance success rates in the cryopreservation of oocytes from mussels and other marine invertebrate species.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105288"},"PeriodicalIF":2.1,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144766769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of procyanidin supplementation on Turkey semen quality, oxidative stress parameters, and fertilization during preservation at −196 °C","authors":"Seyed Sattar Seyed Torabi ,&nbsp;Tohid Mohammadi ,&nbsp;Mehdi Rezaei","doi":"10.1016/j.cryobiol.2025.105286","DOIUrl":"10.1016/j.cryobiol.2025.105286","url":null,"abstract":"<div><div>This study investigated the effects of procyanidin (PC)-supplemented semen extenders on the quality and fertility of cryopreserved turkey sperm. Semen was pooled and diluted using a glucose-based extender enriched with PC at concentrations of 10, 30, and 50 μg before freezing. Post-thaw evaluations included motility, motion characteristics, viability, plasma membrane functionality (PMF), DNA integrity, total antioxidant capacity (TAC), glutathione peroxidase (GPx) activity, superoxide dismutase (SOD) activity, and lipid peroxidation (MDA) levels. Fertility and hatchability assessments were conducted via artificial insemination using thawed semen. Extenders with 30 and 50 μg PC significantly enhanced motility, viability, PMF, TAC, and SOD activity while reducing DNA damage, abnormal morphology, and lipid peroxidation compared to other groups. Moreover, fertility rates were notably higher with PC concentrations of 30 and 50 μg. The findings highlight that adding PC at 30 or 50 μg improves the quality of cryopreserved turkey semen, optimizing fertility and hatchability outcomes.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105286"},"PeriodicalIF":2.1,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144748791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryopreservation of Centropomus viridis gonadal tissue: A key step for conservation and aquaculture in Mexico","authors":"Galilea Fonseca-González ,&nbsp;Lucia Suárez-López ,&nbsp;Marisela Aguilar-Juárez ,&nbsp;Carmen G. Paniagua-Chávez","doi":"10.1016/j.cryobiol.2025.105285","DOIUrl":"10.1016/j.cryobiol.2025.105285","url":null,"abstract":"<div><div>The snook (<em>Centropomus viridis</em>) has a significant economic impact in Mexico's northern Pacific coastal region. Germ cell (GCs) cryopreservation is used as a strategy for species conservation due to its ability to transfer genetic information, promote self-renewal, and facilitate cellular differentiation, ensuring the propagation of the species. In this work, we present a protocol for the cryopreservation of gonadal tissue from juvenile snook (<em>C. viridis</em>). Gonadal tissue disaggregation was performed using trypsin (0.25 %, 0.30 %, 0.40 %, or 0.50 %). GCs were isolated using a Percoll density gradient (10 % and 40 %). Cryopreservation was performed using ethylene glycol and dimethyl sulfoxide (DMSO) at concentrations of 1.5 M or 2 M or the combination of 1.5 M DMSO, lactose (0.1 or 0.2 M), and 10 % egg yolk. Freezing was performed at −80 °C for 10, 15, or 30 min. GCs were identified using <em>vasa</em> by immunocytochemistry. Cell viability and concentration were assessed. Trypsin at 0.25 % resulted in a 98.25 ± 1.0 % cell viability. GCs were isolated from the 10 % Percoll band, with 36 % of the cells expressing <em>vasa</em>. Gonadal tissue cryopreserved with 2 M DMSO at −80 °C for 15 min exhibited the highest cell viability, with 71.83 ± 2.47 %. The results establish a baseline for guiding future cryopreservation efforts involving gonadal tissue from other marine fish species.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105285"},"PeriodicalIF":2.3,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the recovery rate of vitrified hatched blastocysts and the frequency of blebbing between different thawing protocols","authors":"Tsuyoshi Okubo,&nbsp;Ai Higuchi,&nbsp;Kenta Higuchi,&nbsp;Tomomi Taguchi,&nbsp;Ryoko Matsuo,&nbsp;Noriyuki Onda,&nbsp;Teruaki Hayashi,&nbsp;Kenji Omi,&nbsp;Tomoya Segawa","doi":"10.1016/j.cryobiol.2025.105284","DOIUrl":"10.1016/j.cryobiol.2025.105284","url":null,"abstract":"<div><div>This study evaluated the effects of different warming protocols on the recovery rate and blebbing frequency of vitrified hatched blastocysts, aiming to optimize embryo warming methods for improved clinical outcomes. A total of 650 patients (857 cycles) were included, with blastocysts warmed using either the conventional multi-step dilution method or a rapid warming method involving direct immersion in a low-concentration sucrose solution. Recovery rate, blebbing frequency, implantation rate, and clinical pregnancy rate were compared, along with the relationship to embryo morphology at the time of freezing. While recovery rates were similar between the two methods (conventional: 123.67 %, rapid: 124.45 %), the rapid method significantly reduced blebbing frequency (5.1 % vs. 10.9 %, P &lt; 0.05). Implantation and clinical pregnancy rates were similar between the two groups. However, the rapid warming method effectively reduces blebbing while maintaining recovery rates. These findings suggest that rapid warming may improve embryo stability by reducing osmotic stress, supporting its potential clinical benefit and the need for further studies on long-term outcomes such as live birth rates.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105284"},"PeriodicalIF":2.3,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maximizing sleep quality and well-being in female athletes: the role of the menstrual cycle and whole-body cryostimulation","authors":"Lynda Messaoudène ,&nbsp;Coralie Arc-Chagnaud ,&nbsp;Floriane Renier,&nbsp;Quentin Bretonneau,&nbsp;Laurent Bosquet,&nbsp;Nathalie Delpech,&nbsp;DDay Consortium,&nbsp;Carina Enea","doi":"10.1016/j.cryobiol.2025.105283","DOIUrl":"10.1016/j.cryobiol.2025.105283","url":null,"abstract":"<div><div>The aim of this study was to evaluate the effects of two menstrual cycle (MC) phases - early follicular phase (EFP) and mid-luteal phase (MLP) - on sleep, well-being, and the effects of whole-body cryostimulation (WBC) in young female athletes. Core temperature (CT) and heart rate variability (HRV) were also assessed to explore the physiological mechanisms underlying these effects, both under control conditions and after WBC. Using a randomized, controlled, cross-over design, seventeen naturally menstruating women (24 ± 3.3 years) were evaluated over two consecutive cycles (cycle length 21–35 days, positive urinary ovulation test). The experimental cycle included two three-day WBC sessions (3 min at −110 °C) compared to a control cycle without WBC. Subjective and objective sleep quality (SQ), CT, and HRV were measured during both cycles. The MC phase did not significantly affect subjective SQ, but was associated with decreased objective SQ and increased fatigue during the MLP compared to the EFP. The MLP was also characterized by elevated CT and increased sympathetic activity, suggesting impaired nocturnal thermoregulation during this phase. WBC improved objective SQ and perceived fatigue during the MLP and appeared to help restore thermoregulatory function, although sympathetic activity remained elevated. These findings suggest that WBC may be an effective intervention to counteract MC-related impairments in sleep and fatigue in female athletes.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105283"},"PeriodicalIF":2.3,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expert consensus on cryosurgery therapy of mucosal melanoma on head and neck","authors":"Yadong Wu ,&nbsp;Guoxin Ren ,&nbsp;Moyi Sun ,&nbsp;Zhangui Tang ,&nbsp;Longjiang Li ,&nbsp;Jian Meng ,&nbsp;Zhijun Sun ,&nbsp;Shaoyan Liu ,&nbsp;Yue He ,&nbsp;Wei Shang ,&nbsp;Bo Li ,&nbsp;Jie Zhang ,&nbsp;Heming Wu ,&nbsp;Yi Li ,&nbsp;Shaohui Huang ,&nbsp;Jiong Lü ,&nbsp;Zhongcheng Gong ,&nbsp;Jun Wang ,&nbsp;Anxun Wang ,&nbsp;Zhiyong Li ,&nbsp;Wei Guo","doi":"10.1016/j.cryobiol.2025.105280","DOIUrl":"10.1016/j.cryobiol.2025.105280","url":null,"abstract":"<div><div>Cryoablation therapy for tumors has a long history of clinical application. Its anti-tumor mechanisms and histopathological changes have been well established, with extensive clinical practice demonstrating its safety and efficacy, theoretically making it an ideal modality for tumor treatment. Historically constrained by limitations in cryogenic media and freezing equipment, its therapeutic effectiveness and clinical adoption were significantly restricted. The emergence of new-generation cryoablation systems represented by Argon-Helium cryosurgical systems has achieved substantial advancements in refrigeration efficiency, ablation range precision, and temperature monitoring accuracy, thereby greatly promoting the widespread adoption of tumor cryoablation technology. This consensus systematically summarizes the mechanisms of cryoablation technology, indications for cryotherapy in head and neck mucosal melanoma, standardized clinical treatment protocols, management of adverse reactions, and related principles. It aims to provide authoritative references for standardizing cryoablation therapy in the treatment of head and neck mucosal melanoma.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105280"},"PeriodicalIF":2.3,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144611680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DFT-based evaluation of cryoprotectants and their role in lyophilization success","authors":"Mariia S. Ashikhmina ,&nbsp;Pavel V. Nesterov ,&nbsp;Vladislav S. Filozop ,&nbsp;Mikhail O. Volodarskiy ,&nbsp;Olga Y. Orlova ,&nbsp;Michael Nosonovsky ,&nbsp;Alexander S. Novikov ,&nbsp;Ekaterina V. Skorb","doi":"10.1016/j.cryobiol.2025.105282","DOIUrl":"10.1016/j.cryobiol.2025.105282","url":null,"abstract":"<div><div>Cryopreservation and lyophilization are essential for preserving and transporting biological samples, including bacterial cultures. These processes face severe challenges due to the formation of ice crystals that can damage cellular structures. Cryoprotectants (CPAs) are vital in reducing this damage by inhibiting ice nucleation and growth. The present study examines the use of various CPAs, focusing on their ability to enhance the survival of <em>Bacillus coagulans</em> and <em>Streptococcus thermophilus</em> during cryo-freezing and lyophilization. Using density functional theory (DFT) calculations, we analyzed interactions between water molecules. We selected CPAs (sucrose, fructosofuranose, glucose, fructose, and glycerol) to predict their effectiveness in forming protective hydrate shells around bacterial cells. Our results indicate that sucrose, which has a low Gibbs free energy of solvation at low temperatures, provides the most effective cryoprotection. Experimental validation showed that a 12 % sucrose solution improves bacterial survival after lyophilization. We also investigated the effect of different freezing protocols on bacterial survival. Initial freezing at −18 °C and subsequent storage at −80 °C were the optimal methods, maximizing cell survival. The findings contribute to a deeper understanding of cryopreservation and lyophilization processes, with potential applications in biotechnology and biomedical fields.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105282"},"PeriodicalIF":2.3,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144569739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}