Cryobiology最新文献

{"title":"Regulatory mechanisms and application prospects of lipid metabolites in ovarian vitrification-induced cryoinjury.","authors":"Ruoxi Wu, Hui Ren, Jing Ma, Yan Zhang, Qiang Chen, Xinfeng Liu","doi":"10.1016/j.cryobiol.2026.105721","DOIUrl":"https://doi.org/10.1016/j.cryobiol.2026.105721","url":null,"abstract":"<p><p>Ovarian vitrification is a core technology for female fertility preservation, ovarian function protection before chemoradiotherapy in cancer patients, and germplasm conservation in rare animals. Compared with slow freezing, vitrification is widely adopted for producing fewer ice crystals, causing less cryoinjury, and being operationally efficient. However, cold stress, abrupt osmotic shifts, and excessive oxidative stress during vitrification still induce ovarian cell apoptosis, plasma membrane disruption, and mitochondrial dysfunction, markedly reducing post-thaw survival and function. Lipids are essential structural components of ovarian tissue, oocyte plasma membranes, and organelle membranes; lipid metabolic homeostasis directly determines cellular cryotolerance and post-thaw developmental potential. Recent lipidomics studies have demonstrated that lipid metabolites such as fatty acids, cholesterol, sphingolipids, and carnitine alleviate ovarian vitrification-induced injury and improve cryopreservation outcomes by regulating membrane stability, oxidative stress, mitochondrial function, and follicular apoptotic signaling pathways. This review summarizes the mechanisms of lipid metabolism in mediating ovarian vitrification-induced cryoinjury, the regulatory roles of major lipid metabolites, key pre-freezing intervention strategies targeting ovarian lipid metabolism, current technological bottlenecks, and future prospects of lipid-targeted regulation in ovarian cryopreservation and fertility preservation. The aim is to provide a theoretical basis and technical reference for optimizing vitrification systems and improving thawed ovarian tissue quality.</p>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"125 ","pages":"105721"},"PeriodicalIF":2.6,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149223449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Cryopreservation of Aurelia aurita larvae, a new model for understanding cryobiology in high-water content marine species” [Cryobiology 123 (2026) 105628]","authors":"Alba Lago,&nbsp;Jesus Troncoso,&nbsp;Estefania Paredes","doi":"10.1016/j.cryobiol.2026.105667","DOIUrl":"10.1016/j.cryobiol.2026.105667","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"124 ","pages":"Article 105667"},"PeriodicalIF":2.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148270538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of techniques for cryopreserving giant clam larvae","authors":"Raul Emid Castillo,&nbsp;Sujune Tsai,&nbsp;Bonien Chen,&nbsp;Zhi-Hong Wen,&nbsp;Chiahsin Lin","doi":"10.1016/j.cryobiol.2026.105698","DOIUrl":"10.1016/j.cryobiol.2026.105698","url":null,"abstract":"<div><div>Giant clams (<em>Tridacna</em> spp.) are keystone reef organisms that play important ecological roles in Indo-Pacific coral reef ecosystems. However, their populations have declined substantially because of overexploitation, habitat degradation, and climate change. Cryopreservation offers a potential strategy for preserving genetic resources and supporting future conservation and restoration efforts. In this study, we evaluated the feasibility of cryopreserving D-stage larvae of <em>Tridacna crocea</em> and <em>T</em>. <em>maxima</em> using a two-step freezing protocol. The effects of different cryoprotectants, concentrations, and equilibration times on post-thaw larval integrity and motility were assessed. Larval development was monitored using microscale culture techniques and microscopic observations. The results demonstrated species-specific responses to cryopreservation treatments, with <em>T. maxima</em> generally exhibiting greater cryotolerance than <em>T. crocea</em>. Ethylene glycol produced the highest larval integrity percentage (LIP) in both species. In addition, glycerol and propylene glycol yielded relatively high LIP values in <em>T. crocea</em>, whereas methanol and dimethyl sulfoxide (DMSO) performed more effectively in <em>T. maxima</em>. Post-thaw motility was observed only in a limited number of treatment groups, with the highest motility recorded in larvae treated with 0.75 M glycerol for 30 min and 0.5 M DMSO for 20 min. In contrast, non-cryopreserved control larvae exhibited a progressive decline in motility during culture, decreasing to 12.1% in <em>T. maxima</em> and 4.0% in <em>T. crocea</em> after 24 h. To our knowledge, this study represents the first investigation of cryopreservation in tridacnid D-stage larvae. Although post-thaw motility remained limited, the successful preservation of larval structural integrity demonstrates the potential of cryopreservation as a conservation tool for giant clams. Further optimization of species-specific cryopreservation protocols will be necessary to improve post-thaw recovery and support long-term genetic resource banking.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"124 ","pages":"Article 105698"},"PeriodicalIF":2.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In memoriam Kenneth B. Storey – Cryobiologist","authors":"Shannon N. Tessier ,&nbsp;Boris Rubinsky ,&nbsp;John G. Baust ,&nbsp;Jennifer E. Storey","doi":"10.1016/j.cryobiol.2026.105646","DOIUrl":"10.1016/j.cryobiol.2026.105646","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"123 ","pages":"Article 105646"},"PeriodicalIF":2.1,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148177818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryopreservation of Aurelia aurita larvae, a new model for understanding cryobiology in high-water content marine species.","authors":"Alba Lago, Jesus Troncoso, Estefania Paredes","doi":"10.1016/j.cryobiol.2026.105628","DOIUrl":"10.1016/j.cryobiol.2026.105628","url":null,"abstract":"<p><p>Cryopreservation of marine species is a key tool for biodiversity conservation, population management, and the advance of aquaculture and biological research. Currently exist significant progress in the cryopreservation of gametes, embryos, larvae and even juveniles of several genus of marine invertebrates. However, cnidarians, and particularly jellyfish, remain largely underexplored. In this study, we report on the first successful cryopreservation of Aurelia aurita ephyrae, the first larval stage of this jellyfish species. This species is characterized by extremely high-water content (96.3 %), which represents a unique challenge in the method of cryopreservation. In this study we done toxicity tests using different concentrations ranging from 0.5 to 6 M of dimethyl sulfoxide (Me₂SO). On the other hand, we designed two cocktails of cryoprotectants and evaluated the efficiency in a protocol of cryopreservation. The first one is based on 1 M Me₂SO + 0.5 M dimethylformamide (DMF) and the second used 1.5 M Me₂SO with 0.04 M trehalose (TRE) and 1 % BSA as a post-thaw supplement. The first protocol achieved 100 % survival immediately after thawing and 33 % survival at 48 h, while the second maintained a consistent 33 % survival across both time points. The damage and viability of the larvae was assessed using live/dead fluorescence dye and allowed confirming the integrity of surviving individuals. These results mark the first demonstration of successful cryopreservation protocol for jellyfish larvae and represent a significant advance for the ex-situ conservation of gelatinous zooplankton. This work lays the foundation for further research into cryopreservation protocols for marine species, understand most better water flow for organisms with high water content and opens new possibilities for marine biodiversity conservation and cnidarian model systems.</p>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"123 ","pages":"105628"},"PeriodicalIF":2.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147615819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of cryopreservation on pollen viability and morphological integrity of five Indian Crinum species","authors":"Harshid Pulparambil ,&nbsp;P.E. Rajasekharan ,&nbsp;N.S. Pradeep","doi":"10.1016/j.cryobiol.2026.105586","DOIUrl":"10.1016/j.cryobiol.2026.105586","url":null,"abstract":"<div><div><em>Crinum</em> species are ornamental plants of high horticultural value and medicinal importance, known for their bioactive alkaloids with neurological and anticancer properties. Sustainable use and genetic conservation of <em>Crinum</em> require reliable long-term germplasm conservation strategies, and pollen cryopreservation offers an effective approach by enabling year-round breeding and conservation independent of seasonal and geographic constraints. This study presents the first species-comparative evaluation of pollen germination behaviour and cryopreservation responses in five ecologically contrasting Indian <em>Crinum</em> species (<em>C. asiaticum</em>, <em>C. latifolium, C. malabaricum</em>, <em>C. lorifolium and C. viviparum</em>). <em>In vitro</em> pollen germination treatments with varying sucrose (1–10 %) and polyethylene glycol (PEG) concentrations were optimized, species-specific critical moisture contents were determined, and pollen viability was assessed before and after cryostorage at −196 °C for up to 180 days. Distinct, habitat-linked germination responses were observed: aquatic and semi-aquatic species achieved optimal germination (&gt;91 %) only under PEG-mediated osmotic regulation, whereas terrestrial species performed best in sucrose-only treatments. Precise moisture content ranges (12–18 %) were identified as critical for maximizing post-cryopreservation viability, with small deviations resulting in marked germination loss. Cryopreserved pollen retained high functional competence, with minimal viability decline (&lt;5 %) after 180 days, and scanning electron microscopy revealed only minor, non-critical ultrastructural changes. By linking pollen osmotic sensitivity, ecological adaptation, and cryotolerance within a single genus, this study advances pollen cryobiology beyond empirical optimization and provides biologically informed, species-specific protocols for pollen cryobanking. These findings strengthen the scientific basis for <em>ex situ</em> conservation, germplasm exchange, and breeding programmes targeting horticultural and medicinal improvement of <em>Crinum</em> species.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"122 ","pages":"Article 105586"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced prevention of cell death by hypothermic storage with propyl gallate","authors":"Xiaodong Li ,&nbsp;Ryan Chan ,&nbsp;Xinglan Li ,&nbsp;Qiunong Guan ,&nbsp;Jingyi Li ,&nbsp;Xiaowei Li ,&nbsp;Haofeng Li ,&nbsp;Shadan Li ,&nbsp;Caigan Du","doi":"10.1016/j.cryobiol.2025.105562","DOIUrl":"10.1016/j.cryobiol.2025.105562","url":null,"abstract":"<div><div>Current hypothermic preservation solutions <strong>are</strong> insufficient to prevent donor organ injury <strong>during hypothermic</strong> storage, which negatively impacts graft functional recovery and survival after transplantation. This study was to evaluate whether supplementation of the preservation solutions with antioxidant propyl gallates (PG) reduced cell injury during hypothermic storage and subsequently rewarming-reoxygenation or transplantation. Human cells and rat aorta were stored in University of Wisconsin (UW) or hyperbranched polyglycerol (HPG) solution supplemented with PG at 4 °C, followed by rewarming-reoxygenation with atmospheric O₂ at 37 °C and isotransplantation, respectively. Cell or tissue injury was measured by lactate dehydrogenase release, flow cytometric and histologic analyses. Here, we showed that PG supplementation significantly increased antioxidative activities of both UW and HPG solutions, and its concentrations for maximum protection of different human cells from hypothermic storage-induced cell death were estimated between 25 and 50 μM, which was also indicated by increased cell survival or decreased cell apoptosis during rewarming-reoxygenation. The PG cytoprotection was correlated with its inhibition of lipid peroxidation, prevention of mitochondrial dysfunction or an increase in ATP content. The enhanced cytoprotection of preservation solutions by PG supplementation was confirmed in hypothermic storage of rat aortas, indicated by less tissue damage and higher levels of tissue ATP during the hypothermic storage and less chronic injury after transplantation. In c<strong>onclusion, cell injury during hypothermic</strong> storage and subsequently rewarming-reoxygenation was substantially prevented by the hypothermic preservation with PG, suggesting that PG is a promising antioxidant for hypothermic preservation of donor cells or organs in transplantation.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"122 ","pages":"Article 105562"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145615983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of whole-body cryostimulation treatments on the profile of selected adipokines and sirtuins in middle-aged individuals with varying degrees of body fatness","authors":"Adrianna Dzidek ,&nbsp;Olga Czerwińska-Ledwig ,&nbsp;Roxana Zuziak ,&nbsp;Joanna Kryst ,&nbsp;Marta Morawiec ,&nbsp;Zbigniew Szyguła ,&nbsp;Tomasz Pałka ,&nbsp;Anna Kurkiewicz-Piotrowska","doi":"10.1016/j.cryobiol.2026.105599","DOIUrl":"10.1016/j.cryobiol.2026.105599","url":null,"abstract":"<div><div>Whole-body cryostimulation (WBC), a widely utilized intervention involving exposure to extremely low temperatures, elicits a range of physiological responses. Growing evidence suggests that WBC may serve as a complementary, non-pharmacological strategy for obesity management and the modulation of metabolic homeostasis. The aim of the study was to evaluate the effects of a series of WBC sessions on the profile of selected adipokines, sirtuins, and carbohydrate metabolism in individuals with normal and elevated BMI. A total of 23 volunteers participated in the study: 12 in the study group (SG; BMI &gt;27) and 11 in the control group (CG; BMI 18.5-25). Blood samples were collected: prior to the 1st (I), after the 9th (II), after the 19th (III) WBC treatment, and two weeks following the cessation of the intervention (IV). The WBC series had a positive impact on fasting blood glucose (I vs II <em>p=</em>0.035; I vs III <em>p=</em>0.039), glycated hemoglobin (I vs IV <em>p=</em>0.004), and asprosin (I vs IV <em>p</em> = 0.020). A positive effect was observed in SG on levels of: asprosin (I vs IV <em>p &lt;</em> 0.001), SIRT1 (<em>p &lt;</em> 0.001), insulin (I vs III <em>p=</em>0.041, I vs IV <em>p=</em>0.013), and HOMA-IR (I vs III <em>p=</em>0.009, I vs IV <em>p=</em>0.009). The WBC series lowered leptin concentrations in SG (I vs III <em>p=</em>0.013) and CG (I vs III <em>p=</em>0.005). However, the downward trend did not continue in SG after the intervention was discontinued. A series of WBC sessions improved carbohydrate metabolism and modulated adipokine and sirtuin profiles, with the most pronounced effects observed in individuals with elevated BMI. However, this effect generally may require a longer treatment series.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"122 ","pages":"Article 105599"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147316791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The post-thaw quality, antioxidant activity, and in vivo fertility of Zaraibi buck semen frozen-stored in the presence of different concentrations of either quercetin or L-arginine","authors":"Essam A. Almadaly ,&nbsp;Mohamed A. Elbendary ,&nbsp;Samia M. Abd El-Rheem ,&nbsp;Ahmed M. Shehabeldin ,&nbsp;Wael B. El-Domany ,&nbsp;Adel A. Ramoun","doi":"10.1016/j.cryobiol.2025.105580","DOIUrl":"10.1016/j.cryobiol.2025.105580","url":null,"abstract":"<div><div>Over the last decade, there has been a growing interest in incorporating specific additives into buck semen extenders to enhance their semen quality, as their antioxidant defense against oxidative stress and lipid peroxidation is insufficient. Due to their strong antioxidant capabilities, quercetin (QUE) and L-arginine (LA) have garnered considerable interest among these additives. Therefore, this study investigates the effect of adding various concentrations of either QUE or LA in the cryopreservation extender on the post-thaw sperm characteristics, kinematics, enzymatic antioxidant activity, and <em>in vivo</em> fertility of buck semen. Ejaculates were collected from 9 healthy <em>Zaraibi</em> bucks using an electroejaculator once a week. Good-quality ejaculates were pooled and dispensed into 7 aliquots; each aliquot was diluted with Tris-egg yolk citrate extender containing: 1) 10 μM QUE; 2) 15 μM QUE; 3) 30 μM QUE; 4) 50 μM QUE; 5) 2 mM LA; 6) 4 mM LA; and 7) the last aliquot was not supplemented with any additive and set as a control (CTRL). Diluted semen samples were equilibrated at 4 °C for 4 h, loaded into French mini straws, sealed, and frozen-stored in liquid nitrogen. Frozen straws were thawed and examined for sperm characteristics and kinematics; also, enzymatic antioxidants, including catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX), and total antioxidant capacity (TAC), as well as the lipid peroxidation marker malondialdehyde (MDA), were determined. A total of 210 (30 female/group) mature and healthy doe-goats were selected, exposed to estrus synchronization, and inseminated by the prepared frozen-thawed straws to calculate their <em>in vivo</em> fertility rates. The obtained findings revealed that the 30 μM QUE, 50 μM QUE, and 4 mM LA groups had the highest proportions (P &lt; 0.05) of all post-thaw sperm characteristics. Only the 30 μM QUE group had the greatest (P &lt; 0.05) values of all sperm kinematics. Frozen-thawed buck semen's enzymatic antioxidant activity was markedly enhanced by adding either 30 μM QUE or 4 mM LA into the semen extender. The <em>in vivo</em> fertility rates of frozen-thawed straws enriched with either 30 μM QUE (73.33 %) or 4 mM LA (70.00 %) were higher than those of other treatments and the control group (43.33 %). In conclusion, adding either 30 μM QUE or 4 mM LA to the buck semen cryopreservation extender is recommended to improve its post-thaw sperm quality, antioxidant activity, and <em>in vivo</em> fertility.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"122 ","pages":"Article 105580"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145910919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of storage conditions on viability of hematopoietic stem cells and leukocyte subpopulations in fresh and cryopreserved umbilical cord blood samples","authors":"Vladimira Rimac ,&nbsp;Sanja Mazić ,&nbsp;Ines Bojanić","doi":"10.1016/j.cryobiol.2025.105576","DOIUrl":"10.1016/j.cryobiol.2025.105576","url":null,"abstract":"<div><div>Umbilical cord blood (UCB) units are cryopreserved for long-term storage, but it is still not fully investigated how cryopreservation and freezing affect the viability of different cell types. This prospective study evaluated the stability of fresh and cryopreserved UCB samples in various storage conditions using the 7-AAD/annexin V method. UCBs were collected “ex-utero” and processed according to the institutional standard operating procedures. In the stability study, fresh UCB buffy coat (BC) samples were stored at +4 °C and room temperature (RT) for up to 24 h, while thawed samples for up to one and 2 h. After a defined time period, cells were labelled again and analysed. Early apoptosis was most prevalent in CD34⁺ cells and least in T lymphocytes in both fresh and thawed samples. The highest post-thaw recovery was observed for T lymphocytes. The total CD19⁺ (P = 0.001) and CD16+/56+ (P = 0.004) cell counts were statistically significantly reduced when fresh samples were stored at RT. Total NK cell counts were also reduced when samples were stored at +4 °C (P = 0.036). In cryopreserved samples, there was statistically significant differences in total cell counts for all cell populations when samples were stored at RT, and under these storage conditions, early apoptosis of B and NK cells also occurred. The results showed different post-thaw recoveries of leukocyte subpopulations in the samples of cryopreserved UCB units. Cell exposure to the cryoprotective solution post-thaw does not affect total cell count or further development of apoptosis when UCB samples are stored at +4 °C for up to 2 h.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"122 ","pages":"Article 105576"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145862249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}