Cryobiology最新文献

{"title":"Numerical simulation of bioheat transfer during bronchial cryobiopsy using cryoprobes of different diameters","authors":"Junhong Tang ,&nbsp;Yudong Bao ,&nbsp;Wenqing Du ,&nbsp;Wen Wei","doi":"10.1016/j.cryobiol.2025.105320","DOIUrl":"10.1016/j.cryobiol.2025.105320","url":null,"abstract":"<div><div>Transbronchial cryobiopsy using flexible cryoprobes is an emerging biopsy technique. However, an inappropriately sized cryoprobe or inaccurate freezing time may result in tissue cryoinjury or substandard tissue specimens. This study uses numerical simulation to explore the effects of different diameter cryoprobes (1.1 mm, 1.9 mm, 2.4 mm) on tissue temperature distribution and phase transition in bronchial tumor cryobiopsy. A three-dimensional bronchial model containing tumors is constructed. The heat transfer process of the cryoprobes within 0–10 s at different insertion depths (0–1.0 mm, interval 0.1 mm) is simulated based on Pennes bioheat equation and the effective heat capacity method. Multi-physics effects are analyzed by coupling respiratory airflow. The results demonstrate that increasing the cryoprobe diameter and its insertion depth leads to an expansion of the low-temperature zone within the tissue, thereby elevating the risk of cryoinjury to surrounding peritumoral tissues. Moreover, the effect of cryoprobe diameter on tissue phase transition is more significant than that of insertion depth. Increasing the cryoprobe diameter will reduce the distance to adjacent peritumoral tissues, resulting in rapid expansion of the frozen region within peritumoral tissues. In contrast, the insertion depth primarily influences the axial extension of the frozen region. Additionally, respiratory airflow demonstrates no significant impact on temperature distribution. This study provides a theoretical foundation for the clinical selection of cryoprobe parameters and optimization of freezing duration, facilitating the acquisition of sufficient tissue samples while minimizing cryoinjury to healthy tissues.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105320"},"PeriodicalIF":2.1,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of the cryotop vitrification method for the cryopreservation of in vitro produced Cỏ goat embryos","authors":"Van Khanh Nguyen ,&nbsp;Huong Thi Thu Vu ,&nbsp;Au Thi Hoang ,&nbsp;Yen Thi Kim Pham ,&nbsp;Dat Van LE ,&nbsp;Lan Anh Thi Nguyen ,&nbsp;Nguyen Thi Nhien ,&nbsp;Nguyen Thi Van Anh ,&nbsp;Lan Doan Pham","doi":"10.1016/j.cryobiol.2025.105323","DOIUrl":"10.1016/j.cryobiol.2025.105323","url":null,"abstract":"<div><div>This study investigated the influence of embryonic developmental stage, cryoprotectant concentration, and vitrification solution volume on the cryopreservation efficiency of <em>in vitro</em>-produced Cỏ goat embryos. In <em>Experiment 1</em> the survival rate of zygotes cryopreserved 20 h after IVF was lower than blastocysts cryopreserved 7 days after IVF (66.86 % vs 90.92 %, P &lt; 0.05). The cleavage and blastocyst rates of the zygote group were lower than those of the control (non-cryopreserved) group (52.91 % and 81.94 % vs 81.94 % and 36.48 %, respectively, P &lt; 0.05). The hatching blastocyst rate achieved by embryos vitrified as blastocyst or zygotes was 14.62 % and 3.69 %, respectively. In <em>Experiment 2</em>, the survival rate of blastocysts vitrified in the 16.5 % EG + 16.5 % DMSO group was higher than that of the 12.5 % EG + 12.5 % DMSO and 20 % EG + 20 % DMSO groups (91.34 % vs 43.16 % and 61.24 %, respectively, P &lt; 0.05). The hatching blastocyst rate of 12.5 % EG + 12.5 % DMSO, 16.5 % EG + 16.5 % DMSO and 20 % EG + 20 % DMSO groups was 0 %; 13.74 % and 0 %, respectively. In <em>Experiment 3</em>, the difference in the survival rate between blastocysts vitrified in 0.3 μL, 0.5 μL and 1 μL was not statistically significant (P &gt; 0.05). The hatching blastocyst rate of the 0.3 μL group was higher than that of the 0.5 μL and 1 μL groups (44.18 % vs 13.98 % and 14.36 %, respectively, P &lt; 0.05). In conclusion, the IVP Cỏ goat embryos were successfully vitrified by cryotop vitrification method at a CPA concentration of 16.5 % EG + 16.5 % DMSO with a vitrification solution volume of 0.3 μL.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105323"},"PeriodicalIF":2.1,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving drone sperm cryopreservation: Investigating cryoprotectant combinations and PVP supplementation","authors":"Abdulkadir Kaya ,&nbsp;Ongun Uysal ,&nbsp;Numan Akyol","doi":"10.1016/j.cryobiol.2025.105318","DOIUrl":"10.1016/j.cryobiol.2025.105318","url":null,"abstract":"<div><div>Cryopreservation of drone sperm is a widely studied method for long-term genetic preservation and offers key advantages. However, despite successful cryopreservation, fertilization rates remain unsatisfactory. Artificial insemination with cryopreserved sperm reduces spermatozoa concentration in the queen's spermatheca, highlighting the need for alternative cryoprotectants and extenders. Research on other species suggests that combining cryoprotectants may enhance preservation outcomes compared to using them individually. This study evaluated the effects of various cryoprotectant combinations on drone sperm quality after cryopreservation. Phase I assessed the individual and combined effects of dimethyl sulfoxide (DMSO), glycerol (GLY), dimethylacetamide (DMA), and ethylene glycol (EG). In Phase II, different concentrations of polyvinylpyrrolidone (PVP) were added to DMSO. Finally, the three best combinations were tested in vivo for sperm concentration and plasma membrane integrity (PMI) in queen spermathecae following artificial insemination. DMSO-based cryoprotectants, particularly combinations with EG, GLY, and PVP, significantly enhanced post-thaw sperm quality (P ≤ 0.001). In Phase I, DMSO, EG, and DMSO + EG yielded the highest motility and PMI values. In Phase II, DMSO+6 % PVP achieved optimal results in all parameters. In Phase III, although all cryopreserved groups showed lower spermathecal sperm concentration and PMI compared to fresh semen, the DMSO + PVP group performed best compared to the other cryopreserved treatments. In conclusion, the combined use of cryoprotectants yielded superior cryopreservation outcomes compared to their individual utilization. DMSO showed greater efficacy than GLY, DMA, and EG, while PVP supplementation showed promise in improving drone sperm cryopreservation, warranting further investigation with expanded parameters.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105318"},"PeriodicalIF":2.1,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of cryoprotectant agents and their concentrations on the post-thaw quality of alpaca (Vicugna pacos) epididymal spermatozoa","authors":"Alexei Santiani ,&nbsp;Martha Ugarelli ,&nbsp;Luis Ruiz ,&nbsp;Omar Quispicondor ,&nbsp;Caroline Duymovich ,&nbsp;Jane M. Morrell ,&nbsp;Shirley Evangelista-Vargas","doi":"10.1016/j.cryobiol.2025.105317","DOIUrl":"10.1016/j.cryobiol.2025.105317","url":null,"abstract":"<div><div>Cryopreservation of spermatozoa requires the use of cryoprotective agents (CPAs) to minimize membrane damage and preserve cell function after thawing. In South American camelids, glycerol (GL), ethylene glycol (EG), and dimethyl sulfoxide (DMSO) are the most widely used CPAs. However, some studies in other species have explored the potential of amide-based CPAs, such as dimethylacetamide (DMA), dimethylformamide (DMF), and methylformamide (MF). This study aimed to evaluate the interaction between CPA type and concentration on post-thaw sperm quality in alpacas using a 6 × 3 factorial design (six CPAs × three concentrations: 1 %, 3.5 %, and 7 %). Post-thaw assessments included total motility (bright-field microscopy), viability (SYBR14/PI), and mitochondrial membrane potential (MMP) (MitoTracker Deep Red), with the latter two measured by imaging flow cytometry. Data were analyzed using two-way ANOVA to determine main effects and interactions. CPA concentrations of 1 % and 3.5 % produced significantly higher values for motility, viability, and MMP compared to 7 % (P &lt; 0.05). DMSO and GL exhibited significantly higher post-thaw motility (P &lt; 0.05) than DMA and DMF, although no significant differences among CPAs were observed for viability or MMP. In conclusion, CPA concentration has a greater impact than CPA type on post-thaw sperm quality, and concentrations between 1 % and 3.5 % are optimal for preserving motility, viability, and mitochondrial function in alpaca epididymal spermatozoa.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105317"},"PeriodicalIF":2.1,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ice-crystal formation during cooling and rewarming of concentrated sucrose solutions","authors":"Norihito Kimizuka","doi":"10.1016/j.cryobiol.2025.105301","DOIUrl":"10.1016/j.cryobiol.2025.105301","url":null,"abstract":"<div><div>The concentration dependence of ice-crystal formation during cooling and rewarming of sucrose solutions (51–72 wt%) was studied by differential scanning calorimetry and optical microscopy at cooling and heating rates of ±1.0–±3.0 °C/min. During cooling, ice crystals formed in solutions with concentrations of up to 60 wt%, but were not found in solutions with concentrations of ≥61 wt%. In contrast, ice-crystal at solution concentrations of 59–62 wt% during rewarming was irregular and accompanied by devitrification (<em>T</em>_d) and/or recrystallization (<em>T</em>_r). However, at ≥64 wt%, only devitrification occurred, and no ice crystals were formed at 72 wt% or higher. These results suggest that the <em>T</em>_d curve on the binary system state diagram exists at concentrations of ≥60 wt%, with concentrations of ≥64 wt% resulting in ice-crystal formation only via devitrification at −40 to −30 °C during rewarming at 3.0 °C/min.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105301"},"PeriodicalIF":2.1,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-rapid freezing in spheres yields a higher cryoresistance than in straws but remains inferior to conventional slow freezing of stallion sperm","authors":"Francisco Larriva-González ,&nbsp;Jefferson E. Erráez-Guaicha ,&nbsp;Karen E. Torres-Ordóñez ,&nbsp;Mauricio Duma ,&nbsp;Sebastián Larriva-Salazar ,&nbsp;Diego A. Galarza","doi":"10.1016/j.cryobiol.2025.105303","DOIUrl":"10.1016/j.cryobiol.2025.105303","url":null,"abstract":"<div><div>This study evaluated the cryoresistance of stallion sperm frozen by ultra-rapid (UR) methods using microspheres and straws or by the conventionally-slow (CS) method. Sixteen ejaculates from four stallions were each divided into three aliquots according to the freezing method: UR freezing in 30-μL spheres (UR-Spheres) by direct immersion in liquid nitrogen (LN₂); UR freezing in 0.25-mL straws (UR-Straws) by direct horizontal submersion in LN₂; and CS freezing in LN₂ vapors. Ultra-rapid freezing medium included 100 mM trehalose +1 % BSA, and the CS freezing medium contained 5 % dimethylformamide. Conventional-slow freezing yielded higher sperm cryoresistance than both UR freezing methods (P &lt; 0.05). UR-Spheres produced higher values of sperm kinematic, viability, and acrosomal integrity than UR-Straws (P &lt; 0.05). Sperm head area and perimeter in both UR freezing groups increased compared to fresh and CS frozen samples (P &lt; 0.05). In conclusion, the UR-Spheres method produced greater cryoresistance of stallion semen than UR-Straw method.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105303"},"PeriodicalIF":2.1,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphatidylserine translocation, cholesterol spatial distribution, and acrosome reaction reliably distinguish sperm capacitation from cryoinjury in bovine sperm","authors":"Laura Nataly Garcia-Oliveros ,&nbsp;Alexandre da Rocha Bozzi ,&nbsp;Thais de Oliveira Cardoso Silva ,&nbsp;Fernanda Baatsch Nascimento ,&nbsp;Thainara Rodrigues de Oliveira ,&nbsp;Ellen Lara Miguel ,&nbsp;Maíra Bianchi Rodrigues Alves ,&nbsp;Raissa Braido Rangel ,&nbsp;André Furugen Cesar de Andrade ,&nbsp;Rubens Paes de Arruda ,&nbsp;Karl Kerns ,&nbsp;Eneiva Carla Carvalho Celeghini","doi":"10.1016/j.cryobiol.2025.105302","DOIUrl":"10.1016/j.cryobiol.2025.105302","url":null,"abstract":"<div><div>Sperm capacitation is a critical process for successful fertilization, involving multiple regulated cellular changes. On the other hand, cryopreservation induces membrane changes that can mimic capacitation, potentially leading to misinterpretation of sperm function. Distinguishing true capacitation from cryoinjury remains challenging, as both share surface markers despite involving distinct mechanisms and impacts on fertilization. This study aimed to assess the effectiveness of various techniques in detecting key capacitation-related events and differentiating them from cryopreservation-induced injuries. Semen samples from twelve bulls (three ejaculates each; n = 36) were collected and divided into control (CO) and <em>in vitro</em> capacitated (CAP) groups. All samples were analyzed for sperm concentration, motility characteristics, and capacitation-associated events – including membrane lipid disorder, lipid peroxidation, phosphatidylserine translocation, cholesterol redistribution, acrosome reaction, and mitochondrial membrane potential. Following the initial analysis, semen was cryopreserved, thawed, and then split into cryopreserved control (CRYCO) and capacitated (CRYCAP) groups for reanalysis. Data were evaluated using ANOVA, with means compared by Tukey's test (significance level ≤5 %). Correlations among all measured variables were also examined. Capacitation induction decreased curvilinear velocity, while increasing straightness, phosphatidylserine translocation, cholesterol efflux, and acrosome-reacted sperm, regardless of cryopreservation status. In contrast, plasma membrane disorganization and lipid peroxidation were markedly elevated, and cholesterol redistribution was reduced after cryopreservation. Notably, a strong positive correlation was observed between phosphatidylserine translocation and acrosome reaction in both fresh and cryopreserved sperm. In conclusion, phosphatidylserine translocation, cholesterol spatial distribution, and the acrosome reaction were the most reliable markers for distinguishing true capacitation from cryoinjury.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105302"},"PeriodicalIF":2.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplate-in-a-Box: thermophysical exploration of cold storage high-throughput microplate designs for enhanced rate control in cryopreservation","authors":"B.M. Guerreiro ,&nbsp;J.C. Lima ,&nbsp;J.C. Silva ,&nbsp;F. Freitas","doi":"10.1016/j.cryobiol.2025.105298","DOIUrl":"10.1016/j.cryobiol.2025.105298","url":null,"abstract":"<div><div>High-throughput experimental screening is desirable to minimize data acquisition time from vast workloads. Cell cryopreservation experiments are routinely performed in single-sample cryovials despite cell seeding being performed in 96-well microplates because these substrates are known to induce microliter supercooling, are prone to thermal compressibility and their lengthy preparation period extends cell exposure time to potentially cytotoxic cryoprotectants. Rather than improving the methodological preciseness of cooling, latest efforts have focused on refining cryoprotectant formulations and supplement precautionary ice nucleators. Here, we built 16 microplate-in-a-box cold storage apparatus by iterative design which allow multi-sample slow freezing cryopreservation in 96-well microplates while ensuring the biologically optimal and reproducible cooling rate (1–3 °C/min) required to minimize the deleterious effects of intracellular and extracellular ice formation. The optimal recipient, a 31.9 × 25.8 × 20.5 cm, 4.3 cm thick Styrofoam recipient pre-equilibrated at −80 °C with internal Styrofoam insulation, yielded a linear −1.2 °C/min cooling rate with minimal variability (±0.2), absent of non-linear thermal lag. Throughput increased by 5.3-fold for single and 10.7-fold for double microplate setups. The most impactful features were recipient thermal pre-equilibration before microplate insertion (IR = 7.0 ± 0.5), Styrofoam insulation of the microplate (IR = 6.9 ± 0.2), increased recipient wall thickness (IR = 5.8 ± 0.5), microplate elevation inside the apparatus (IR = 3.5 ± 0.1) and microplate pre-equilibration (IR = 2.7 ± 0.2), all of which contributed to an attenuation of heat exchange mechanisms. The development of easy-to-use, easy-to-build apparatus with common laboratory materials that significantly enables an enhancement of cooling rate control, improving throughput, reproducibility and procedural uniformity is essential to minimizing the impact of non-biological factors in post-thaw cell viability.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105298"},"PeriodicalIF":2.1,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144925922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Ex vivo use of a Rho-kinase inhibitor during renal preservation improves graft function upon reperfusion” [Cryobiology 70 (2015) 71–75]","authors":"Carmen Kirchner ,&nbsp;Bastian Lüer ,&nbsp;Patrik Efferz ,&nbsp;Jeremias Wohlschlaeger ,&nbsp;Andreas Paul ,&nbsp;Thomas Minor","doi":"10.1016/j.cryobiol.2025.105255","DOIUrl":"10.1016/j.cryobiol.2025.105255","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105255"},"PeriodicalIF":2.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards blood on demand: Rapid post-thaw isolation of red blood cells from multicomponent cryoprotectants","authors":"Thomas L.C. Palmer-Dench ,&nbsp;Thomas F. Whale ,&nbsp;Katherine J. Kearney ,&nbsp;Matthew Hindle ,&nbsp;Alex D. Murray ,&nbsp;Thomas R. Congdon ,&nbsp;Matthew I. Gibson ,&nbsp;Fraser L. Macrae","doi":"10.1016/j.cryobiol.2025.105295","DOIUrl":"10.1016/j.cryobiol.2025.105295","url":null,"abstract":"<div><div>Severe blood loss due to trauma, anaemia, or chemotherapy necessitates immediate red blood cell (RBC) transfusions. The short shelf-life of RBCs at 4 °C complicates emergency supply management. Glycerol is the state-of-the-art cryopreservative for RBCs but the time from thawing to transfusion is more than 1 hour, due to the slow, extensive washing process. This presents a major barrier to blood on demand in emergency or military situations. Here we demonstrate that polyampholytes combined with DMSO and trehalose can cryopreserve human RBCs and allow rapid washout in under 30 minutes. Post wash-out, preserved RBCs exhibit comparable viability, morphological integrity, and function to glycerol-preserved RBCs. This method enhances processing and handling, facilitating the use of frozen RBCs in healthcare, military, and other fields reliant on constant donation. Rapid-washout solutions could unlock blood-on-demand from cryopreserved stocks, improving cold-chain management and reducing reliance on walking donors.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105295"},"PeriodicalIF":2.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144924707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}