Cryobiology最新文献

{"title":"Towards blood on demand: Rapid post-thaw isolation of red blood cells from multicomponent cryoprotectants","authors":"Thomas L.C. Palmer-Dench ,&nbsp;Thomas F. Whale ,&nbsp;Katherine J. Kearney ,&nbsp;Matthew Hindle ,&nbsp;Alex D. Murray ,&nbsp;Thomas R. Congdon ,&nbsp;Matthew I. Gibson ,&nbsp;Fraser L. Macrae","doi":"10.1016/j.cryobiol.2025.105295","DOIUrl":"10.1016/j.cryobiol.2025.105295","url":null,"abstract":"<div><div>Severe blood loss due to trauma, anaemia, or chemotherapy necessitates immediate red blood cell (RBC) transfusions. The short shelf-life of RBCs at 4 °C complicates emergency supply management. Glycerol is the state-of-the-art cryopreservative for RBCs but the time from thawing to transfusion is more than 1 hour, due to the slow, extensive washing process. This presents a major barrier to blood on demand in emergency or military situations. Here we demonstrate that polyampholytes combined with DMSO and trehalose can cryopreserve human RBCs and allow rapid washout in under 30 minutes. Post wash-out, preserved RBCs exhibit comparable viability, morphological integrity, and function to glycerol-preserved RBCs. This method enhances processing and handling, facilitating the use of frozen RBCs in healthcare, military, and other fields reliant on constant donation. Rapid-washout solutions could unlock blood-on-demand from cryopreserved stocks, improving cold-chain management and reducing reliance on walking donors.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105295"},"PeriodicalIF":2.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144924707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quercetin enhances the cold storage effect on human umbilical cord mesenchymal stem cells in Celsior solution","authors":"Peng Wang ,&nbsp;Liang Ye ,&nbsp;Shanwen Si","doi":"10.1016/j.cryobiol.2025.105299","DOIUrl":"10.1016/j.cryobiol.2025.105299","url":null,"abstract":"<div><div>Mesenchymal stem cells (MSCs) are being increasingly applied in regenerative medicine and tissue engineering, with proven therapeutic value in different <em>in vivo</em> models. However, the clinical use of mesenchymal stem cells (MSCs) requires an effective short-term preservation method to provide time for cells to be transported from their production facilities to clinical destinations. This study revealed that after 5 days of hypothermic storage at 4 °C followed by 1-h rewarming, MSCs preserved in quercetin-supplemented Celsior solution (a common organ hypothermic preservation solution) exhibited significantly higher viability compared to those in Celsior solution alone. this addition reduced the proliferation ability and did not alter the immune phenotype or multipotent differentiation ability of the MSCs. The underlying mechanisms involved antioxidant stress modulation (elevated SOD activity and reduced ROS and MDA levels) and metabolic support (increased ATP production). The above findings prove that adding quercetin to the Celsior solution serves as a feasible method for the short-term preservation of mesenchymal stem cells.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105299"},"PeriodicalIF":2.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144922747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Memoriam - William F. Rall: Impacting science, impacting people","authors":"Gregory M. Fahy ,&nbsp;Maureen Wood ,&nbsp;Mary Hagedorn","doi":"10.1016/j.cryobiol.2025.105292","DOIUrl":"10.1016/j.cryobiol.2025.105292","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105292"},"PeriodicalIF":2.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Memoriam: Ernest G. Cravalho, Ph.D., (1939–2021)","authors":"Kenneth R. Diller ,&nbsp;John J. McGrath","doi":"10.1016/j.cryobiol.2025.105294","DOIUrl":"10.1016/j.cryobiol.2025.105294","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105294"},"PeriodicalIF":2.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145007348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Osmotic injury and cytotoxicity for hMSCs in contact with Me2SO: The effect of cell size distribution” [Cryobiology 116 (2024) 104943]","authors":"Gabriele Traversari ,&nbsp;Jakub Stas ,&nbsp;Antonio Mario Locci ,&nbsp;Alessandro Concas ,&nbsp;Nicola Lai ,&nbsp;Alberto Cincotti","doi":"10.1016/j.cryobiol.2025.105289","DOIUrl":"10.1016/j.cryobiol.2025.105289","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105289"},"PeriodicalIF":2.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frozen sperm viability and seminiferous epithelium cytology of the coati (Nasua nasua)","authors":"F.F. Souza ,&nbsp;J.L.A. Martins ,&nbsp;O.O. Afolabi ,&nbsp;C.S. Paranzini ,&nbsp;D.P. Leme ,&nbsp;M.S.O.M. Silva ,&nbsp;V.F. Daros ,&nbsp;V.H. Chirinéia ,&nbsp;M.D. Lopes","doi":"10.1016/j.cryobiol.2025.105297","DOIUrl":"10.1016/j.cryobiol.2025.105297","url":null,"abstract":"<div><div>This study aimed to describe the cells of the seminiferous epithelium collected by fine needle aspiration cytology (FNAC), reproductive tract observations, semen collection, and sperm cryopreservation from coatis (<em>Nasua nasua</em>). Seven adult coatis were anesthetized, the reproductive tract was examined, and semen was collected by electroejaculation and cryopreserved using an extender based on protocol for domestic dogs, except that prior to cryopreservation, no centrifugation to remove the seminal plasma was performed. Testicular FNAC was also performed on each testis. The germ cells of seminiferous epithelium were identified and had cell characteristics similar to other species except for the secondary spermatocyte which was not present. Semen samples were collected from four animals, three of which were suitable for cryopreservation, although their seminal characteristics slightly declined after freezing. Examination of the coati reproductive tract revealed well developed penile bone and preputial glands like other carnivores. The testicular length x width x height meant 2.13 × 1.7 × 1.7 cm and testicular volume meant 4.5 cm³. The sperm morphology of coati exhibited an angular head shape, which was evident in the membrane integrity fluorescence assessment. The sperm motility was between 20 and 70 %, and the concentration was between 14 and 129 × 10⁶ sperm/mL with motility of 30.0 ± 17.32 % <em>vs</em> 23.33 ± 11.55 % between samples thawed at 46 °C/15s and 37 °C/30s. In conclusion, testicular FNAC can be a useful tool for evaluating testicular function based on sperm production. Our findings demonstrate that semen collection and cryopreservation in coatis can be achieved using electroejaculation, extenders, and cryopreservation protocols similar to those used for domestic dogs. While these results provide a template for semen cryopreservation in other Procyonidae species, further investigation is needed to determine the necessity of removing seminal plasma.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105297"},"PeriodicalIF":2.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144924702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metformin as a protective supplement enhances survival and stemness maintenance of human dental pulp stem cells during cryopreservation","authors":"Lerong Yu ,&nbsp;Ziyao Zhou ,&nbsp;Mengyu Chen ,&nbsp;Xiangwei Li","doi":"10.1016/j.cryobiol.2025.105300","DOIUrl":"10.1016/j.cryobiol.2025.105300","url":null,"abstract":"<div><div>This study explored the protective effects of metformin on human dental pulp stem cells (hDPSCs) during cryopreservation. hDPSCs were isolated from extracted third molars and treated with varying concentrations of metformin to identify the optimal dose for cryoprotection. Among the tested concentrations, 800 μM was found to effectively reduce reactive oxygen species without compromising cell viability. When used during cryopreservation, metformin significantly improved post-thaw survival, enhanced cell proliferation, and increased colony-forming efficiency. Importantly, it also upregulated key stemness-related genes, including <em>OCT4, NANOG, SOX2,</em> and <em>KLF4</em>. Even under repeated freeze-thaw stress, metformin mitigated functional decline, helping to preserve both cell viability and stem cell characteristics. These findings highlight metformin's potential as a protective supplement to enhance the preservation and clinical utility of hDPSCs.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105300"},"PeriodicalIF":2.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144922746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryoprotective effects of trehalose on the physicochemical and microstructural stability of frozen apple purée","authors":"K. Monalisa,&nbsp;Md Nasim,&nbsp;Rowshon Ara,&nbsp;M.Z. Islam,&nbsp;M.M. Hoque","doi":"10.1016/j.cryobiol.2025.105296","DOIUrl":"10.1016/j.cryobiol.2025.105296","url":null,"abstract":"<div><div>Freezing is a common preservation method for fruit-based products but often leads to quality degradation caused by ice crystal formation and structural damage. This study aimed to evaluate the cryoprotective effects of trehalose on the physicochemical and microstructural stability of apple purée during 30 days of frozen storage at −18 °C. Apple purée samples were prepared with trehalose at concentrations of 0 %, 1 %, and 5 % (w/w) to investigate its efficacy in reducing drip loss, preserving microstructure, and maintaining nutritional and textural quality. Key quality attributes assessed included drip loss, moisture retention, texture-related properties, and microstructure, in addition to evaluating ash content, pH, total soluble solids (TSS), total phenolic content (TPC), antioxidant capacity (DPPH), and color parameters. Results indicated that trehalose significantly mitigated drip loss, with the 5 % treatment achieving the lowest value (6.0 %) compared to the control (14.8 %), suggesting enhanced water-binding capacity and reduced ice crystal-induced cellular damage. Microscopic analysis revealed that trehalose-treated samples exhibited reduced structural collapse and preserved parenchymal integrity, which contributed to improved textural consistency post-thaw. Furthermore, samples with trehalose showed better retention of TPC, antioxidant activity, and color, highlighting trehalose's protective role against oxidative and thermal degradation. These findings underscore the potential of trehalose as a cryoprotective agent in frozen fruit systems, with direct implications for improving the quality of minimally processed fruit products through structural stabilization and moisture management.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105296"},"PeriodicalIF":2.1,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the effect of adding different levels of selenium nanoparticles doped with zinc oxide nanoparticles, and selenium nanoparticles doped with zinc oxide nanoparticles coated with Resveratrol on post-thawing quality of ram semen","authors":"Laya Rastegari Pouyani ,&nbsp;Hadi Hajarian ,&nbsp;Leila Soltani ,&nbsp;Mojtaba Dashtizad","doi":"10.1016/j.cryobiol.2025.105290","DOIUrl":"10.1016/j.cryobiol.2025.105290","url":null,"abstract":"<div><div>Reactive oxygen species (ROS) can potentially cause harm to sperm following cryopreservation. The primary defense against this oxidative damage is antioxidants. One powerful antioxidant is resveratrol (Res), a stilbenoid that belongs to the family of natural phenols and polyphenols. Res is found in various foods, including mulberries, blueberries, raspberries, and grape skins. Antioxidants are essential for protecting sperm because free radicals negatively impact oxidative stress processes. However, the effectiveness of Res is limited due to its poor bioavailability. To address this limitation, loading strategies that utilize nanoparticles and nanotechnology can be employed. In this study, barberry extract was used to create selenium nanoparticles doped with zinc oxide nanoparticles (NSe-do-NZn), which were then coated with Res (NSe-do-NZn-co-Res). Energy-dispersive X-ray spectroscopy (EDX), UV–visible spectroscopy, field emission scanning electron microscopy (FE-SEM), zeta potential analysis, and Fourier-transform infrared spectroscopy (FTIR) were among the tests used to validate the production of nanoparticles. This study aims to investigate the effects of Res-coated NSe-do-NZn on ram sperm parameters after the thawing. Following thawing, various sperm parameters were assessed, including malondialdehyde (MDA) concentration, total motility, viability, plasma membrane, DNA integrity, and abnormalities. The results showed that the addition of 5 μg/ml of NSe-do-NZn-co-Res significantly enhanced sperm survival and total motility during thawing compared to other groups (P &lt; 0.05). Furthermore, this treatment notably improved the percentage of DNA integrity and cell membrane functionality compared to other groups, except for the group receiving 5 μg/ml NSe-do-NZn and the 25 μg/ml Res group (P &lt; 0.05). Similar trends were observed for the reduction in MDA levels and sperm abnormalities. The most significant outcomes were associated with the use of resveratrol coated onto NSe-do-NZn.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105290"},"PeriodicalIF":2.1,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144864718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An efficient and convenient method for the preservation of germplasm resources: Cryopreservation of mature testis of large yellow croaker (Larimichthys crocea)","authors":"Feiyan Li ,&nbsp;Li Zhou ,&nbsp;Kunhuang Han ,&nbsp;Qi Wei ,&nbsp;Meixia Chen ,&nbsp;Zhaohan Sun ,&nbsp;Jia Chen ,&nbsp;Xiaoquan Jiang","doi":"10.1016/j.cryobiol.2025.105293","DOIUrl":"10.1016/j.cryobiol.2025.105293","url":null,"abstract":"<div><div>Large yellow croaker (<em>Larimichthys crocea</em>) is one of the most important marine economic fish. However, due to overfishing and long-term artificial breeding, the germplasm has degraded in recent years, and the wild germplasm has been listed as critically endangered. It is urgent to protect the germplasm resources of <em>Larimichthys crocea</em>. Cryopreservation may be an alternative way, however, the toxicity of cryoprotectants and the complicated preservation procedure that uses a programmable freezer has greatly increased the difficulty of cryopreservation. Therefore, in this study, we aimed to explore an efficient, convenient, inexpensive and low toxicity method for cryopreservation of mature testes in this species. The cryoprotectants of dimethyl sulfoxide (Me₂SO), ethylene glycol (EG), propylene glycol (PG) were selected to cryopreserve the mature testes with liquid nitrogen in simple style. Finally, the testes were cut into blocks (1.0 cm³) with cryoprotectants of 20 % PG and 15 % Me₂SO, placed at a position 10 cm above the liquid nitrogen for 10 min, then plunged into liquid nitrogen, resulting in high survival rate of 64.17 % at 3 days post cryopreservation. And the recovery rate of spermatogonia was more than 70 %. Subsequently, the cells viability were further detected by qRT-PCR, <em>in situ</em> hybridization (ISH) and germ cell transplantation. These results suggested that the spermatogonia from cryopreserved testes had high viability, which could migrate and colonized after transplantation. The present study first established cryopreservation protocols of mature testis in <em>Larimichthys crocea</em>, which will serve as a promising tool to protect Sciaenidae and other fish germplasm resources.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105293"},"PeriodicalIF":2.1,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144864717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}