Towards blood on demand: Rapid post-thaw isolation of red blood cells from multicomponent cryoprotectants

IF 2.1 3区 生物学 Q2 BIOLOGY
Thomas L.C. Palmer-Dench , Thomas F. Whale , Katherine J. Kearney , Matthew Hindle , Alex D. Murray , Thomas R. Congdon , Matthew I. Gibson , Fraser L. Macrae
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引用次数: 0

Abstract

Severe blood loss due to trauma, anaemia, or chemotherapy necessitates immediate red blood cell (RBC) transfusions. The short shelf-life of RBCs at 4 °C complicates emergency supply management. Glycerol is the state-of-the-art cryopreservative for RBCs but the time from thawing to transfusion is more than 1 hour, due to the slow, extensive washing process. This presents a major barrier to blood on demand in emergency or military situations. Here we demonstrate that polyampholytes combined with DMSO and trehalose can cryopreserve human RBCs and allow rapid washout in under 30 minutes. Post wash-out, preserved RBCs exhibit comparable viability, morphological integrity, and function to glycerol-preserved RBCs. This method enhances processing and handling, facilitating the use of frozen RBCs in healthcare, military, and other fields reliant on constant donation. Rapid-washout solutions could unlock blood-on-demand from cryopreserved stocks, improving cold-chain management and reducing reliance on walking donors.

Abstract Image

面向血液需求:从多组分冷冻保护剂中快速分离解冻后红细胞
由于创伤、贫血或化疗导致的严重失血需要立即输入红细胞。红细胞在4°C时的保质期短,使应急供应管理复杂化。甘油是最先进的红细胞冷冻保存剂,但由于缓慢而广泛的清洗过程,从解冻到输血的时间超过1小时。这是在紧急情况或军事情况下按需供应血液的主要障碍。在这里,我们证明了多两性溶解物与DMSO和海藻糖结合可以低温保存人类红细胞,并在30分钟内快速冲洗。水洗后,保存的红细胞表现出与甘油保存的红细胞相当的活力、形态完整性和功能。这种方法加强了处理和处理,促进了冷冻红细胞在医疗保健、军事和其他依赖持续捐赠的领域的使用。快速冲洗解决方案可以从冷冻储备中按需释放血液,改善冷链管理,减少对步行献血者的依赖。
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来源期刊
Cryobiology
Cryobiology 生物-生理学
CiteScore
5.40
自引率
7.40%
发文量
71
审稿时长
56 days
期刊介绍: Cryobiology: International Journal of Low Temperature Biology and Medicine publishes research articles on all aspects of low temperature biology and medicine. Research Areas include: • Cryoprotective additives and their pharmacological actions • Cryosurgery • Freeze-drying • Freezing • Frost hardiness in plants • Hibernation • Hypothermia • Medical applications of reduced temperature • Perfusion of organs • All pertinent methodologies Cryobiology is the official journal of the Society for Cryobiology.
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