二甲双胍作为一种保护性补充剂，可提高人牙髓干细胞在冷冻保存过程中的存活率和干细胞的维持

IF 2.1 3区生物学 Q2 BIOLOGY

Cryobiology Pub Date : 2025-09-01 DOI:10.1016/j.cryobiol.2025.105300

Lerong Yu , Ziyao Zhou , Mengyu Chen , Xiangwei Li

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引用次数: 0

摘要

本研究探讨了二甲双胍对人牙髓干细胞（hDPSCs）冷冻保存的保护作用。从提取的第三磨牙中分离出hdpsc，用不同浓度的二甲双胍处理，以确定冷冻保护的最佳剂量。在测试浓度中，800 μM可以有效地减少活性氧而不影响细胞活力。在冷冻保存期间，二甲双胍显著提高解冻后的存活率，增强细胞增殖，提高集落形成效率。重要的是，它还上调了关键的干细胞相关基因，包括OCT4、NANOG、SOX2和KLF4。即使在反复冻融胁迫下，二甲双胍也能减轻功能衰退，有助于保持细胞活力和干细胞特性。这些发现强调了二甲双胍作为一种保护性补充剂的潜力，可以增强hdpsc的保存和临床应用。

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Metformin as a protective supplement enhances survival and stemness maintenance of human dental pulp stem cells during cryopreservation

This study explored the protective effects of metformin on human dental pulp stem cells (hDPSCs) during cryopreservation. hDPSCs were isolated from extracted third molars and treated with varying concentrations of metformin to identify the optimal dose for cryoprotection. Among the tested concentrations, 800 μM was found to effectively reduce reactive oxygen species without compromising cell viability. When used during cryopreservation, metformin significantly improved post-thaw survival, enhanced cell proliferation, and increased colony-forming efficiency. Importantly, it also upregulated key stemness-related genes, including OCT4, NANOG, SOX2, and KLF4. Even under repeated freeze-thaw stress, metformin mitigated functional decline, helping to preserve both cell viability and stem cell characteristics. These findings highlight metformin's potential as a protective supplement to enhance the preservation and clinical utility of hDPSCs.

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来源期刊

Cryobiology 生物-生理学

CiteScore

5.40

自引率

7.40%

发文量

审稿时长

56 days

期刊介绍： Cryobiology: International Journal of Low Temperature Biology and Medicine publishes research articles on all aspects of low temperature biology and medicine. Research Areas include: • Cryoprotective additives and their pharmacological actions • Cryosurgery • Freeze-drying • Freezing • Frost hardiness in plants • Hibernation • Hypothermia • Medical applications of reduced temperature • Perfusion of organs • All pertinent methodologies Cryobiology is the official journal of the Society for Cryobiology.