Cryobiology最新文献_第10页

Debulking cryogenic ablation in the treatment of colorectal cancer unresectable metastases to the liver 治疗结直肠癌肝脏不可切除转移灶的去纤维化低温消融术

IF 2.3 3区生物学

Cryobiology Pub Date : 2024-03-01 DOI: 10.1016/j.cryobiol.2023.104816

Olexii I. Dronov MD, Yelyzaveta Kozachuk MD, PhD, Fedir Prytkov MD

引用次数: 0

Pancreatic metastases cryosurgery 胰腺转移瘤冷冻手术

IF 2.3 3区生物学

Cryobiology Pub Date : 2024-03-01 DOI: 10.1016/j.cryobiol.2023.104818

Alexei V. Chzhao MD , Dmitri A. Ionkin MD , Yulia A. Stepanova MD

引用次数: 0

Extracorporeal cryoimmunology. new scientific discovery in biomedical science and medicine 体外冷冻免疫学。生物医学科学和医学中的科学新发现

IF 2.3 3区生物学

Cryobiology Pub Date : 2024-03-01 DOI: 10.1016/j.cryobiol.2023.104813

Nikolai N. Korpan MD, PhD , Olexii I. Dronov MD , Kecheng Xu MD

引用次数: 0

The clinical application and novel multi-mode thermal therapy technology 临床应用和新型多模式热疗技术

IF 2.3 3区生物学

Cryobiology Pub Date : 2024-03-01 DOI: 10.1016/j.cryobiol.2023.104829

Wentao Li MD

引用次数: 0

Interventional treatment of hilar and mediastinal lymph node metastases 肺门和纵隔淋巴结转移的介入治疗

IF 2.3 3区生物学

Cryobiology Pub Date : 2024-03-01 DOI: 10.1016/j.cryobiol.2023.104823

Hongwu Wang MD

引用次数: 0

MR imaging guided cryoablation for treatment of brain oligometastatic cancer 磁共振成像引导下的冷冻消融治疗脑寡转移癌

IF 2.3 3区生物学

Cryobiology Pub Date : 2024-03-01 DOI: 10.1016/j.cryobiol.2023.104824

Chengli Li MD

引用次数: 0

Effects of apigenin and trans-ferulic acid on microscopic and oxidative stress parameters in the semen of water buffalo bulls during cryopreservation 芹菜甙元和反式阿魏酸对水牛冷冻保存期间精液显微和氧化应激参数的影响

IF 2.7 3区生物学

Cryobiology Pub Date : 2024-02-27 DOI: 10.1016/j.cryobiol.2024.104868

T. Mohammadi , M. hosseinchi Gharehaghaj , A. Alaei Novin

{"title":"Effects of apigenin and trans-ferulic acid on microscopic and oxidative stress parameters in the semen of water buffalo bulls during cryopreservation","authors":"T. Mohammadi , M. hosseinchi Gharehaghaj , A. Alaei Novin","doi":"10.1016/j.cryobiol.2024.104868","DOIUrl":"10.1016/j.cryobiol.2024.104868","url":null,"abstract":"<div><p>Cryopreservation involves exposing sperm to stressful conditions that affect cell viability. The high quality of the Azerbaijani water buffalo's by-products, such as buffalo milk, makes it a species of significant importance. Our focus is on protecting its genetic resources by preserving its sperm, as their numbers will decrease in the coming years and they are at risk of extinction. This study's goal was to ascertain how apigenin (A) and <em>trans</em>-ferulic acid (t-FA) affected the semen quality of Azari water buffalo bulls under cryopreservation. Pooled buffalo sperm (n = 35 ejaculations) were diluted in a Tris-based diluent also containing varying amounts of apigenin (0.2, 0.4, 0.6, and 0.8 mM) and <em>trans</em>-ferulic acid (2.5, 5, 10 and 20 mM). Following a freeze-thaw procedure, samples were assayed for total antioxidant capacity (TAC), catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), glutathione activity (GSH), glutathione peroxidase (GPx), progressive motility and total motility, motility properties, plasma membrane functionality, and viability. Sixty days after insemination, the rectal examination was performed on 38 buffaloes that had undergone sexual breeding to confirm pregnancy. The results of the study show that the addition of A-0.2, A-0.4, and t-FA-10 to buffalo semen increases the percentage of intact plasma membrane, motility, and sperm viability, as well as the levels of GSH, GPx, CAT. and TAC. In addition, there is a decrease in MDA and DNA damage after cryopreservation. Furthermore, the results show that 0.4 mM apigenin significantly increases conception rates compared to the control group. The base extender of Tris supplemented with A (0.4 and 0.2 mM) and t-FA (10 mM) improves the antioxidant indices of both frozen and thawed buffalo sperm, which in turn improves post-thawing sperm quality and <em>in vivo</em> fertility improves buffalo sperm.</p></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139995884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erythropoietin effects on cryopreserved/transplanted cat ovarian tissue: A comparison of two incubation methods 促红细胞生成素对冷冻保存/移植猫卵巢组织的影响：两种培养方法的比较

IF 2.7 3区生物学

Cryobiology Pub Date : 2024-02-27 DOI: 10.1016/j.cryobiol.2024.104861

Isabella M.G. Silva , Aline Q. Rodrigues , Rayane B. Ribeiro , Beatriz A. Aguiar , Anne E.S.P. Marinho , Elisa A.M. Souza , Yasmin B. Ferreira , Victoria C.O. Azevedo , Daniela M. Oliveira , Sônia N. Báo , Jair T. Goulart , Carolina M. Lucci , Fernanda Paulini

{"title":"Erythropoietin effects on cryopreserved/transplanted cat ovarian tissue: A comparison of two incubation methods","authors":"Isabella M.G. Silva , Aline Q. Rodrigues , Rayane B. Ribeiro , Beatriz A. Aguiar , Anne E.S.P. Marinho , Elisa A.M. Souza , Yasmin B. Ferreira , Victoria C.O. Azevedo , Daniela M. Oliveira , Sônia N. Báo , Jair T. Goulart , Carolina M. Lucci , Fernanda Paulini","doi":"10.1016/j.cryobiol.2024.104861","DOIUrl":"10.1016/j.cryobiol.2024.104861","url":null,"abstract":"<div><p>Many feline species are currently threatened with extinction. Therefore, germplasm bank establishment has become imperative. However, cryoinjury and ischemia-reperfusion injury pose significant obstacles to both cryopreservation and xenotransplantation. In this regard, erythropoietin (Epo) represents a potential alternative strategy due to its properties. This study aimed to assess the incubation of domestic cat ovarian tissue in Epo, both before and after cryopreservation, and investigate its effectiveness in promoting revascularization following xenotransplantation. Sixteen ovaries from 8 healthy cats were sliced following elective bilateral ovariohysterectomy (OHE). Subsequently, 8 fragments measuring 3 mm³ each were obtained from the cortical region of each ovary. The fragments were allocated into 3 treatment groups: Cryo group, fragments were cryopreserved, thawed and immediately transplanted; Cryo + Epo group, fragments were first cryopreserved in nitrogen, thawed, incubated in Epo (100 IU) for 2h and transplanted; and the Epo + Cryo group, in which fragments were first incubated in Epo (100 IU) for 2h, cryopreserved, thawed and immediately transplanted. The fragments were then xenotransplanted into the dorsal subcutaneous region of ovariectomized female nude mice and retrieved at 7, 14, 21, and 28 days post-transplantation. The results indicated that Epo effectively enhanced follicular survival, preservation of viability, and tissue revascularization. The Epo + Cryo group displayed better revascularization rates on D14 and D21 post-transplantation and an increase in primordial and growing follicles on D28, the Cryo + Epo group exhibited significantly more follicles on D14 and D21, with fewer degenerated follicles.</p></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139995885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryopreservation of sperm from the gudgeon, Microphysogobio rapidus (Cyprinidae): Effects of cryoprotectant, diluents, and dilution ratio 鳉鱼（鲤科）精子的冷冻保存：冷冻保护剂、稀释剂和稀释比例的影响。

IF 2.7 3区生物学

Cryobiology Pub Date : 2024-02-25 DOI: 10.1016/j.cryobiol.2024.104869

Irfan Zidni , Kang-Won Kim , Hyun-Seok Jang , Moon-Seong Heo , Keun-Sik Kim , Ju-Duk Yoon , Han-Kyu Lim

{"title":"Cryopreservation of sperm from the gudgeon, Microphysogobio rapidus (Cyprinidae): Effects of cryoprotectant, diluents, and dilution ratio","authors":"Irfan Zidni , Kang-Won Kim , Hyun-Seok Jang , Moon-Seong Heo , Keun-Sik Kim , Ju-Duk Yoon , Han-Kyu Lim","doi":"10.1016/j.cryobiol.2024.104869","DOIUrl":"10.1016/j.cryobiol.2024.104869","url":null,"abstract":"<div><p>We investigated methods for cryopreserving sperm from the endangered gudgeon, <em>Microphysogobio rapidus</em>, by examining the effects of cryoprotective agent (CPA) concentration, diluent, and dilution ratio on post-thaw sperm quality. The quality of frozen sperm was evaluated in terms of motility and kinematic parameters, viability, DNA damage, and fertilization rate. We evaluated methanol, glycerol, dimethyl sulfoxide (DMSO), and ethylene glycol as CPAs. Sperm motility, velocity, and viability were significantly higher when methanol was used as the CPA (<em>p</em> < 0.05). The diluents tested were Ringer's solution, Kurokura's Extender, Common Carp Sperm Extender (CCSE), and buffered sperm motility-inhibiting saline solution (BSMIS); post-thaw motility was highest when Ringer's solution was used as the diluent. Next, various quantities of methanol were combined with Ringer's solution to identify the optimal dose of methanol. The dilution ratios tested ranged from 1:1 to 1:7. Cryopreserved sperm was thawed at 20 °C for 15 s. The use of 10% methanol with Ringer's solution at a dilution ratio of 1:5 resulted in the highest post-thaw sperm motility, viability, and velocity including VAP, VCL, and VSL. Post-thaw sperm showed significantly greater DNA damage than the control (fresh sperm) (<em>p</em> < 0.05). The fertilization rate was highest with fresh sperm (<em>p</em> < 0.05), followed by sperm frozen with 10% methanol + Ringer's solution. We recommend that the best way to preserve sperm in the studied species is to use a combination of Ringer's solution and 10% methanol at a 1:5 dilution ratio. Our findings will facilitate the artificial fertilization of <em>M. rapidus</em>.</p></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139982545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryopreservation of human semen by inherently-controlled icing probability: Or how the surface profile of superhydrophobic carbon soot coatings and the sperm volume affect the outcome of slow freezing? 利用固有的可控结冰概率冷冻保存人类精液：或者说超疏水碳烟涂层的表面轮廓和精子体积如何影响缓慢冷冻的结果？

IF 2.7 3区生物学

Cryobiology Pub Date : 2024-02-22 DOI: 10.1016/j.cryobiol.2024.104863

Karekin D. Esmeryan , Todor A. Chaushev

{"title":"Cryopreservation of human semen by inherently-controlled icing probability: Or how the surface profile of superhydrophobic carbon soot coatings and the sperm volume affect the outcome of slow freezing?","authors":"Karekin D. Esmeryan , Todor A. Chaushev","doi":"10.1016/j.cryobiol.2024.104863","DOIUrl":"10.1016/j.cryobiol.2024.104863","url":null,"abstract":"<div><p>The restoration of initial functionality of human spermatozoa subjected to cryopreservation is challenging, because the deleterious intracellular icing and the occurrence of osmotic shocks due to prolonged exposure to increased concentrations of intracellular solutes are oppositely dependent on the cooling rate. This longstanding problem could be overcome if using superhydrophobic soot coatings delaying the heat transfer rate, reducing the ice formation probability and triggering balanced and timely dehydration of the cells, but the effect of their surface profile and sperm volume on the success rate of slow freezing is unclear. Here, we show for the first time that the two-factor freezing injury is entirely avoidable by tailoring the solid-to-gas voids (pores) fraction in the soot, leading to increased nucleation free energy barrier, presumable incipiency of ice crystals with controllable shape and size and hence, fully (100 %) recovered post-thaw sperm motility. It is demonstrated that the reason for such a unique scientific result is the selection of soot coatings with appropriate morphochemical features, hypothetically (not directly proven yet) inducing equilibrium among the solution composition and ice crystals formation, retarding the undesirable compression of liquid-filled “slush ice” channels surrounding the cytoplasm and impeding the ice recrystallization. The novel insights introduced in this article open endless horizon for customizing and revolutionizing the technical protocols in cryobiology.</p></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S001122402400018X/pdfft?md5=b8add1808630cb7b856d9fad4b7a34d6&pid=1-s2.0-S001122402400018X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139939815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0