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Cryopreservation of porcine intact tibial plateau using vitrification 玻璃化法冷冻保存猪胫骨平台
IF 2.1 3区 生物学
Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105399
Korinne M. Tirones , Mohammadhamed Shahsavari , Evan J.C. Andrin , Janet A.W. Elliott , Nadr M. Jomha
{"title":"Cryopreservation of porcine intact tibial plateau using vitrification","authors":"Korinne M. Tirones , Mohammadhamed Shahsavari , Evan J.C. Andrin , Janet A.W. Elliott , Nadr M. Jomha","doi":"10.1016/j.cryobiol.2025.105399","DOIUrl":"10.1016/j.cryobiol.2025.105399","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105399"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cold, chaos, confinement: On the evolving thermodynamics of modern cryopreservation 冷、混沌、约束：论现代低温保存热力学的演变
IF 2.1 3区 生物学
Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105381
Matthew J. Powell-Palm , Anthony N. Consiglio
{"title":"Cold, chaos, confinement: On the evolving thermodynamics of modern cryopreservation","authors":"Matthew J. Powell-Palm , Anthony N. Consiglio","doi":"10.1016/j.cryobiol.2025.105381","DOIUrl":"10.1016/j.cryobiol.2025.105381","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105381"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Research on the role of endoplasmic reticulum-mitochondria interactions in maintaining calcium homeostasis in skeletal muscle fibers of hibernating Daurian ground squirrels 内质网-线粒体相互作用在达斡尔地松鼠冬眠骨骼肌纤维钙稳态维持中的作用研究。
IF 2.1 3区 生物学
Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-10-02 DOI: 10.1016/j.cryobiol.2025.105337
Jie Zhang , Fangyang Pan , Yue He , Jing Zhao , Xiaozhuo Bai , Yanhong Wei , Yunfang Gao
{"title":"Research on the role of endoplasmic reticulum-mitochondria interactions in maintaining calcium homeostasis in skeletal muscle fibers of hibernating Daurian ground squirrels","authors":"Jie Zhang ,&nbsp;Fangyang Pan ,&nbsp;Yue He ,&nbsp;Jing Zhao ,&nbsp;Xiaozhuo Bai ,&nbsp;Yanhong Wei ,&nbsp;Yunfang Gao","doi":"10.1016/j.cryobiol.2025.105337","DOIUrl":"10.1016/j.cryobiol.2025.105337","url":null,"abstract":"<div><div>The ability to maintain cytoplasmic Ca<sup>2+</sup> homeostasis during the torpor-arousal cycle is a crucial strategy that enables hibernators to resist disuse-induced muscle atrophy during hibernation. However, whether ER-mitochondria interactions contribute to the regulation of cytoplasmic Ca<sup>2+</sup> homeostasis in skeletal muscle fibers during hibernation remain unclear. Here, we systematically explored the expression of tethering proteins, Ca<sup>2+</sup> axis-related proteins, and protein complexes involved in Ca<sup>2+</sup> transport within the ER-mitochondria contact sites (ERMCS) of soleus (SOL) and extensor digitorum longus (EDL) muscles in Daurian ground squirrels (<em>Spermophilus dauricus</em>) during hibernation. Results demonstrated a significant increase in the expression of the tethering protein MFN2 and Ca<sup>2+</sup> axis-related proteins GRP75 and VDAC1, as well as enhanced interactions within the Ca<sup>2+</sup> axis complex in the SOL muscle in the inter-bout arousal (IBA) group, as compared to the late torpor (LT) group. In addition, these same indicators exhibited no significant alternation in the EDL muscle during the torpor-arousal cycle. These findings suggest that ER-mitochondria interactions may participate in maintaining Ca<sup>2+</sup> homeostasis in the skeletal muscles during the torpor-arousal cycle, especially in slow-twitch muscle.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105337"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improving drone sperm cryopreservation: Investigating cryoprotectant combinations and PVP supplementation 改进无人机精子冷冻保存：研究冷冻保护剂组合和PVP补充
IF 2.1 3区 生物学
Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-09-12 DOI: 10.1016/j.cryobiol.2025.105318
Abdulkadir Kaya , Ongun Uysal , Numan Akyol
{"title":"Improving drone sperm cryopreservation: Investigating cryoprotectant combinations and PVP supplementation","authors":"Abdulkadir Kaya ,&nbsp;Ongun Uysal ,&nbsp;Numan Akyol","doi":"10.1016/j.cryobiol.2025.105318","DOIUrl":"10.1016/j.cryobiol.2025.105318","url":null,"abstract":"<div><div>Cryopreservation of drone sperm is a widely studied method for long-term genetic preservation and offers key advantages. However, despite successful cryopreservation, fertilization rates remain unsatisfactory. Artificial insemination with cryopreserved sperm reduces spermatozoa concentration in the queen's spermatheca, highlighting the need for alternative cryoprotectants and extenders. Research on other species suggests that combining cryoprotectants may enhance preservation outcomes compared to using them individually. This study evaluated the effects of various cryoprotectant combinations on drone sperm quality after cryopreservation. Phase I assessed the individual and combined effects of dimethyl sulfoxide (DMSO), glycerol (GLY), dimethylacetamide (DMA), and ethylene glycol (EG). In Phase II, different concentrations of polyvinylpyrrolidone (PVP) were added to DMSO. Finally, the three best combinations were tested in vivo for sperm concentration and plasma membrane integrity (PMI) in queen spermathecae following artificial insemination. DMSO-based cryoprotectants, particularly combinations with EG, GLY, and PVP, significantly enhanced post-thaw sperm quality (P ≤ 0.001). In Phase I, DMSO, EG, and DMSO + EG yielded the highest motility and PMI values. In Phase II, DMSO+6 % PVP achieved optimal results in all parameters. In Phase III, although all cryopreserved groups showed lower spermathecal sperm concentration and PMI compared to fresh semen, the DMSO + PVP group performed best compared to the other cryopreserved treatments. In conclusion, the combined use of cryoprotectants yielded superior cryopreservation outcomes compared to their individual utilization. DMSO showed greater efficacy than GLY, DMA, and EG, while PVP supplementation showed promise in improving drone sperm cryopreservation, warranting further investigation with expanded parameters.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105318"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cold-acclimatization prior cryopreservation – A study on ullucu (Ullucus tuberosus) and yacon (Smallanthus sonchifolius) 冷驯化前的低温保存——玉露果（Ullucus tuberosus）和雪莲果（Smallanthus sonchifolius）的研究
IF 2.1 3区 生物学
Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105347
Rainer Vollmer, Iván Manrique, Rosalva Villagaray, Mario Castro, Alexandre Mello, Vania Azevedo
{"title":"Cold-acclimatization prior cryopreservation – A study on ullucu (Ullucus tuberosus) and yacon (Smallanthus sonchifolius)","authors":"Rainer Vollmer,&nbsp;Iván Manrique,&nbsp;Rosalva Villagaray,&nbsp;Mario Castro,&nbsp;Alexandre Mello,&nbsp;Vania Azevedo","doi":"10.1016/j.cryobiol.2025.105347","DOIUrl":"10.1016/j.cryobiol.2025.105347","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105347"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Trends in drying technology of heat sensitive materials 热敏性物料干燥技术的发展趋势
IF 2.1 3区 生物学
Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105377
Petra Foerst, Mathias Hilmer, Isabel Kalinke
{"title":"Trends in drying technology of heat sensitive materials","authors":"Petra Foerst,&nbsp;Mathias Hilmer,&nbsp;Isabel Kalinke","doi":"10.1016/j.cryobiol.2025.105377","DOIUrl":"10.1016/j.cryobiol.2025.105377","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105377"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of cryoprotective agent exposure time and hydrogel application for vitrification of porcine meniscus 冷冻保护剂暴露时间与水凝胶应用于猪半月板玻璃化的比较
IF 2.1 3区 生物学
Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105401
Evan J.C. Andrin , Mohammadhamed Shahsavari , Korinne Tirones , Janet A.W. Elliott , Nadr M. Jomha
{"title":"Comparison of cryoprotective agent exposure time and hydrogel application for vitrification of porcine meniscus","authors":"Evan J.C. Andrin ,&nbsp;Mohammadhamed Shahsavari ,&nbsp;Korinne Tirones ,&nbsp;Janet A.W. Elliott ,&nbsp;Nadr M. Jomha","doi":"10.1016/j.cryobiol.2025.105401","DOIUrl":"10.1016/j.cryobiol.2025.105401","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105401"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Editorial: Plant cryobiotechnology: multidisciplinary and modern research to understand the fundamental basis that allows successful cryopreservation and recovery 社论：植物低温生物技术：多学科和现代研究，了解允许成功低温保存和恢复的基本基础。
IF 2.1 3区 生物学
Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-09-27 DOI: 10.1016/j.cryobiol.2025.105328
Daniel Ballesteros
{"title":"Editorial: Plant cryobiotechnology: multidisciplinary and modern research to understand the fundamental basis that allows successful cryopreservation and recovery","authors":"Daniel Ballesteros","doi":"10.1016/j.cryobiol.2025.105328","DOIUrl":"10.1016/j.cryobiol.2025.105328","url":null,"abstract":"<div><div>This Editorial introduces and contextualizes the papers of the Virtual Special Issue: Plant cryobiotechnology: multidisciplinary and modern research to understand the fundamental basis that allows successful cryopreservation and recovery.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105328"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vegetative desiccation tolerance: A rare phenomenon enabling insights into cryopreservation 植物的干燥耐受性：一个罕见的现象，使见解冷冻保存
IF 2.1 3区 生物学
Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105344
Jill M. Farrant
{"title":"Vegetative desiccation tolerance: A rare phenomenon enabling insights into cryopreservation","authors":"Jill M. Farrant","doi":"10.1016/j.cryobiol.2025.105344","DOIUrl":"10.1016/j.cryobiol.2025.105344","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105344"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spray freeze-drying of inhalable monoclonal antibodies: Enhancing stability and flowability 可吸入性单克隆抗体的喷雾冷冻干燥：增强稳定性和流动性
IF 2.1 3区 生物学
Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105378
Lorena Pasero , Adamo Sulpizi , Tomaso Guidi , Roberto Pisano
{"title":"Spray freeze-drying of inhalable monoclonal antibodies: Enhancing stability and flowability","authors":"Lorena Pasero ,&nbsp;Adamo Sulpizi ,&nbsp;Tomaso Guidi ,&nbsp;Roberto Pisano","doi":"10.1016/j.cryobiol.2025.105378","DOIUrl":"10.1016/j.cryobiol.2025.105378","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105378"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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