Cryobiology最新文献_第9页

Cold-induced mitochondrial alterations in lung epithelial cells are dependent on the extracellular chloride concentration 冷诱导的肺上皮细胞线粒体改变依赖于细胞外氯化物浓度

IF 2.1 3区生物学

Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105406

Dhanusha Schwan , Alexander Klenke , Katharina Kettler , Björn Walter , Mona Schmitz , Ana Jakovljevi , Michaela Schedel , Ursula Rauen

引用次数: 0

TMT-based proteomic analysis reveals key proteins involved in cryoinjury of Nubian goat spermatozoa 基于tmt的蛋白质组学分析揭示了与努比亚山羊精子冷冻损伤相关的关键蛋白

IF 2.1 3区生物学

Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-11-14 DOI: 10.1016/j.cryobiol.2025.105542

Jianwei Zou , Lili Wei , Yishan Liang , Dinghao Xu , Zhihua Mo , Yanna Huang , Qinyang Jiang

{"title":"TMT-based proteomic analysis reveals key proteins involved in cryoinjury of Nubian goat spermatozoa","authors":"Jianwei Zou , Lili Wei , Yishan Liang , Dinghao Xu , Zhihua Mo , Yanna Huang , Qinyang Jiang","doi":"10.1016/j.cryobiol.2025.105542","DOIUrl":"10.1016/j.cryobiol.2025.105542","url":null,"abstract":"<div><div>Cryopreservation is a common assisted reproductive technology in goat breeding, but it often impairs sperm quality and fertilizing ability. This study aimed to explore the effects of cryopreservation on sperm quality and protein abundance profiles in Nubian goat spermatozoa using TMT-based quantitative proteomics. Semen from six healthy adult bucks was divided into fresh and frozen-thawed groups. Key sperm quality parameters, including total motility, membrane integrity, acrosomal integrity, and abnormality rates, were evaluated. TMT-labeled proteomic analysis was performed, followed by GO, KEGG, and PPI network analyses. Results showed that cryopreservation significantly reduced total motility, membrane and acrosomal integrity, while increasing abnormality rates (P < 0.0001). A total of 204 differentially abundant proteins (DAPs) were identified, including 108 upregulated and 96 downregulated proteins. Functional enrichment revealed that these DAPs were mainly involved in glycolysis, cytoskeleton organization, lysosomal function, and oxidative stress. PPI analysis identified GAPDHS, ODF2, and ALB as key proteins potentially regulating sperm cryoinjury. This study provides new insights into the molecular mechanisms of sperm cryodamage in goats and suggests candidate biomarkers for improving cryopreservation strategies.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105542"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145525594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultra-rapid freezing in spheres yields a higher cryoresistance than in straws but remains inferior to conventional slow freezing of stallion sperm 在球体中超高速冷冻产生比在吸管中更高的抗冻性，但仍然不如传统的慢速冷冻的种马精子

IF 2.1 3区生物学

Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-09-08 DOI: 10.1016/j.cryobiol.2025.105303

Francisco Larriva-González , Jefferson E. Erráez-Guaicha , Karen E. Torres-Ordóñez , Mauricio Duma , Sebastián Larriva-Salazar , Diego A. Galarza

{"title":"Ultra-rapid freezing in spheres yields a higher cryoresistance than in straws but remains inferior to conventional slow freezing of stallion sperm","authors":"Francisco Larriva-González , Jefferson E. Erráez-Guaicha , Karen E. Torres-Ordóñez , Mauricio Duma , Sebastián Larriva-Salazar , Diego A. Galarza","doi":"10.1016/j.cryobiol.2025.105303","DOIUrl":"10.1016/j.cryobiol.2025.105303","url":null,"abstract":"<div><div>This study evaluated the cryoresistance of stallion sperm frozen by ultra-rapid (UR) methods using microspheres and straws or by the conventionally-slow (CS) method. Sixteen ejaculates from four stallions were each divided into three aliquots according to the freezing method: UR freezing in 30-μL spheres (UR-Spheres) by direct immersion in liquid nitrogen (LN<sub>2</sub>); UR freezing in 0.25-mL straws (UR-Straws) by direct horizontal submersion in LN<sub>2</sub>; and CS freezing in LN<sub>2</sub> vapors. Ultra-rapid freezing medium included 100 mM trehalose +1 % BSA, and the CS freezing medium contained 5 % dimethylformamide. Conventional-slow freezing yielded higher sperm cryoresistance than both UR freezing methods (P < 0.05). UR-Spheres produced higher values of sperm kinematic, viability, and acrosomal integrity than UR-Straws (P < 0.05). Sperm head area and perimeter in both UR freezing groups increased compared to fresh and CS frozen samples (P < 0.05). In conclusion, the UR-Spheres method produced greater cryoresistance of stallion semen than UR-Straw method.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105303"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of cryoprotectant agents and their concentrations on the post-thaw quality of alpaca (Vicugna pacos) epididymal spermatozoa 冷冻保护剂及其浓度对羊驼附睾精子解冻后质量的影响

IF 2.1 3区生物学

Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-09-11 DOI: 10.1016/j.cryobiol.2025.105317

Alexei Santiani , Martha Ugarelli , Luis Ruiz , Omar Quispicondor , Caroline Duymovich , Jane M. Morrell , Shirley Evangelista-Vargas

{"title":"Comparison of cryoprotectant agents and their concentrations on the post-thaw quality of alpaca (Vicugna pacos) epididymal spermatozoa","authors":"Alexei Santiani , Martha Ugarelli , Luis Ruiz , Omar Quispicondor , Caroline Duymovich , Jane M. Morrell , Shirley Evangelista-Vargas","doi":"10.1016/j.cryobiol.2025.105317","DOIUrl":"10.1016/j.cryobiol.2025.105317","url":null,"abstract":"<div><div>Cryopreservation of spermatozoa requires the use of cryoprotective agents (CPAs) to minimize membrane damage and preserve cell function after thawing. In South American camelids, glycerol (GL), ethylene glycol (EG), and dimethyl sulfoxide (DMSO) are the most widely used CPAs. However, some studies in other species have explored the potential of amide-based CPAs, such as dimethylacetamide (DMA), dimethylformamide (DMF), and methylformamide (MF). This study aimed to evaluate the interaction between CPA type and concentration on post-thaw sperm quality in alpacas using a 6 × 3 factorial design (six CPAs × three concentrations: 1 %, 3.5 %, and 7 %). Post-thaw assessments included total motility (bright-field microscopy), viability (SYBR14/PI), and mitochondrial membrane potential (MMP) (MitoTracker Deep Red), with the latter two measured by imaging flow cytometry. Data were analyzed using two-way ANOVA to determine main effects and interactions. CPA concentrations of 1 % and 3.5 % produced significantly higher values for motility, viability, and MMP compared to 7 % (P < 0.05). DMSO and GL exhibited significantly higher post-thaw motility (P < 0.05) than DMA and DMF, although no significant differences among CPAs were observed for viability or MMP. In conclusion, CPA concentration has a greater impact than CPA type on post-thaw sperm quality, and concentrations between 1 % and 3.5 % are optimal for preserving motility, viability, and mitochondrial function in alpaca epididymal spermatozoa.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105317"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Catechins reduce membrane fragility to enhance red blood cell stability during lyophilization 儿茶素降低了细胞膜的脆弱性，在冻干过程中增强了红细胞的稳定性

IF 2.1 3区生物学

Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105376

Charles A. Elder , Ali M. Alkafaji , Kavin Parthiv , Mohammad Zaidi , Jonathan A. Kopechek , Michael A. Menze

引用次数: 0

Efficient vitrification preservation of cells and tissues based on enhanced heat transfer 基于强化传热的细胞和组织的高效玻璃化保存

IF 2.1 3区生物学

Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105386

Xiaoming Zhou, Peng Zhang

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Cluster crystallization of cryoprotective solutions and associated damage of cryopreserved biosystems 低温保护溶液的团簇结晶和低温保存生物系统的相关损伤

IF 2.1 3区生物学

Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105380

Oleksandr Osetsky

引用次数: 0

Cryopreservation techniques for blue catfish, Ictalurus furcatus, sperm using 2.5 mL straws 蓝色鲶鱼，Ictalurus furcatus，精子低温保存技术，使用2.5 mL吸管。

IF 2.1 3区生物学

Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-10-17 DOI: 10.1016/j.cryobiol.2025.105335

Kyle R. Wood , Brian Bosworth , Samitha S.N. Liyanage , Alexandra E. Nowicki , Kaylan A. Martin , Rex A. Dunham , Benjamin H. Beck , Jason W. Abernathy , Xu Wang , Luke A. Roy , Ian A.E. Butts

{"title":"Cryopreservation techniques for blue catfish, Ictalurus furcatus, sperm using 2.5 mL straws","authors":"Kyle R. Wood , Brian Bosworth , Samitha S.N. Liyanage , Alexandra E. Nowicki , Kaylan A. Martin , Rex A. Dunham , Benjamin H. Beck , Jason W. Abernathy , Xu Wang , Luke A. Roy , Ian A.E. Butts","doi":"10.1016/j.cryobiol.2025.105335","DOIUrl":"10.1016/j.cryobiol.2025.105335","url":null,"abstract":"<div><div>Our objectives were to investigate how permeating cryoprotectant agents (CPAs; dimethyl sulfoxide, methanol), non-permeating CPAs (trehalose at 100 and 300 mmol/L; lactose at 5 and 10 %), and freezing rates (−1.0, −5.0, −10, −20 °C/min) impact post-thaw sperm motility and velocity (VCL) in blue catfish (<em>Ictalurus furcatus</em>) using 2.5 mL straws. Sperm frozen with methanol at −20 °C/min had the highest kinematics (VCL = 141.8 ± 18.5 μm/s, motility = 30.5 ± 12.2 %), and showed similar results to fresh sperm (VCL = 162.7 ± 5.4 μm/s, motility = 50.2 ± 15.1 %). Adding non-permeating CPAs did not improve VCL, and sperm motility was inhibited by lactose 5–10 % and trehalose 300 mmol/L. However, trehalose 100 mmol/L increased motility by 41.2 %, relative to straws without the addition of non-permeating CPAs. Development of this new freezing technology has merit for selective breeding, large research projects, and commercial-scale production for an economically important farm animal.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105335"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145318269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological changes in ovarian follicles, stromal cells, and extracellular matrix in cryopreserved cattle ovarian tissue and the beneficial effects of Croton argyrophyllus Kunth essential oil 低温保存牛卵巢组织中卵泡、间质细胞和细胞外基质的形态学变化及巴豆精油的有益作用。

IF 2.1 3区生物学

Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-10-17 DOI: 10.1016/j.cryobiol.2025.105338

Paloma de Souza Santana , Francisco das Chagas Costa , Francisco Freire Caetano Filho , Vitória Santos Bezerra , Bianca Régia Silva , Andreza de Aguiar Silva , Érica Costa Marcelino , Sueline Cavalcante Chaves , Carlos Eduardo Rodrigues Aguiar , Solano Dantas Martins , Valdevane Rocha Araújo , Geovany Amorim Gomes , José Roberto Viana Silva

{"title":"Morphological changes in ovarian follicles, stromal cells, and extracellular matrix in cryopreserved cattle ovarian tissue and the beneficial effects of Croton argyrophyllus Kunth essential oil","authors":"Paloma de Souza Santana , Francisco das Chagas Costa , Francisco Freire Caetano Filho , Vitória Santos Bezerra , Bianca Régia Silva , Andreza de Aguiar Silva , Érica Costa Marcelino , Sueline Cavalcante Chaves , Carlos Eduardo Rodrigues Aguiar , Solano Dantas Martins , Valdevane Rocha Araújo , Geovany Amorim Gomes , José Roberto Viana Silva","doi":"10.1016/j.cryobiol.2025.105338","DOIUrl":"10.1016/j.cryobiol.2025.105338","url":null,"abstract":"<div><div>This study examined morphological changes in preantral follicles, stromal cells, and the extracellular matrix of cattle ovarian tissue vitrified in medium supplemented with <em>Croton argyrophyllus</em> Kunth essential oil (CaEO). Ovarian cortical slices were cryopreserved using solid surface vitrification in α-MEM<sup>+</sup> alone (control) or supplemented with 0.01, 0.1, 1.0, or 10.0 μg/mL CaEO. After thawing, the ovarian fragments were cultured for 24 h. Then, follicular morphology, stromal cells, collagen distribution, thiol levels, and the activity of enzymatic antioxidants catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPX) were analyzed. Histological analysis revealed an increased rate of healthy follicles in the ovarian tissues vitrified in medium supplemented with CaEO in comparison to the control group. However, no significant differences were found in the rate of primordial follicle activation (P < 0.05). Collagen fibers were more abundant in fragments vitrified with 0.1, 1, or 10 μg/mL CaEO than in those stored with 0.01 μg/mL CaEO or in the control medium (P < 0.05). A higher thiol content was observed in tissues cryopreserved with 1 or 10 μg/mL CaEO than in the control group (P < 0.05). Catalase activity was greater in samples stored in the presence of 0.01 or 0.1 μg/mL CaEO than in tissue cryopreserved with 10 μg/mL CaEO (P < 0.05). In conclusion, CaEO enhances the efficiency of cryopreservation by preserving follicular health, increasing collagen fibers, and regulating antioxidant enzyme activity.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105338"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145318300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Osmotic response of immature equine and bovine oocytes to cryoprotectants at different temperatures 不同温度下未成熟马和牛卵母细胞对冷冻保护剂的渗透反应

IF 2.1 3区生物学

Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105390

Sonia Gago , Judith Diaz-Muñoz , Adam Z. Higgins , Tania García-Martínez , Nuno Costa-Borges , Teresa Mogas

引用次数: 0