Cryobiology最新文献

{"title":"Phosphatidylserine translocation, cholesterol spatial distribution, and acrosome reaction reliably distinguish sperm capacitation from cryoinjury in bovine sperm","authors":"Laura Nataly Garcia-Oliveros ,&nbsp;Alexandre da Rocha Bozzi ,&nbsp;Thais de Oliveira Cardoso Silva ,&nbsp;Fernanda Baatsch Nascimento ,&nbsp;Thainara Rodrigues de Oliveira ,&nbsp;Ellen Lara Miguel ,&nbsp;Maíra Bianchi Rodrigues Alves ,&nbsp;Raissa Braido Rangel ,&nbsp;André Furugen Cesar de Andrade ,&nbsp;Rubens Paes de Arruda ,&nbsp;Karl Kerns ,&nbsp;Eneiva Carla Carvalho Celeghini","doi":"10.1016/j.cryobiol.2025.105302","DOIUrl":"10.1016/j.cryobiol.2025.105302","url":null,"abstract":"<div><div>Sperm capacitation is a critical process for successful fertilization, involving multiple regulated cellular changes. On the other hand, cryopreservation induces membrane changes that can mimic capacitation, potentially leading to misinterpretation of sperm function. Distinguishing true capacitation from cryoinjury remains challenging, as both share surface markers despite involving distinct mechanisms and impacts on fertilization. This study aimed to assess the effectiveness of various techniques in detecting key capacitation-related events and differentiating them from cryopreservation-induced injuries. Semen samples from twelve bulls (three ejaculates each; n = 36) were collected and divided into control (CO) and <em>in vitro</em> capacitated (CAP) groups. All samples were analyzed for sperm concentration, motility characteristics, and capacitation-associated events – including membrane lipid disorder, lipid peroxidation, phosphatidylserine translocation, cholesterol redistribution, acrosome reaction, and mitochondrial membrane potential. Following the initial analysis, semen was cryopreserved, thawed, and then split into cryopreserved control (CRYCO) and capacitated (CRYCAP) groups for reanalysis. Data were evaluated using ANOVA, with means compared by Tukey's test (significance level ≤5 %). Correlations among all measured variables were also examined. Capacitation induction decreased curvilinear velocity, while increasing straightness, phosphatidylserine translocation, cholesterol efflux, and acrosome-reacted sperm, regardless of cryopreservation status. In contrast, plasma membrane disorganization and lipid peroxidation were markedly elevated, and cholesterol redistribution was reduced after cryopreservation. Notably, a strong positive correlation was observed between phosphatidylserine translocation and acrosome reaction in both fresh and cryopreserved sperm. In conclusion, phosphatidylserine translocation, cholesterol spatial distribution, and the acrosome reaction were the most reliable markers for distinguishing true capacitation from cryoinjury.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105302"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isochoric freezing: Optimizing food preservation through controlled temperature and pressure conditions","authors":"Alan L. Maida ,&nbsp;Anthony N. Consiglio ,&nbsp;Cristina Bilbao-Sainz ,&nbsp;Andrew Karman ,&nbsp;Gary Takeoka ,&nbsp;Matthew J. Powell-Palm ,&nbsp;Boris Rubinsky","doi":"10.1016/j.cryobiol.2025.105370","DOIUrl":"10.1016/j.cryobiol.2025.105370","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105370"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenges and advances in the cryopreservation of Paracentrotus lividus eggs","authors":"Sara Campos,&nbsp;Estefanía Paredes,&nbsp;Jesús Troncoso","doi":"10.1016/j.cryobiol.2025.105396","DOIUrl":"10.1016/j.cryobiol.2025.105396","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105396"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the potential of lea protein - Solute interactions for stabilizing biomolecules during drying","authors":"Anja Thalhammer","doi":"10.1016/j.cryobiol.2025.105345","DOIUrl":"10.1016/j.cryobiol.2025.105345","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105345"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryopreservation of human brain vascular pericytes in suspension","authors":"Hadi Afsaneh,&nbsp;Leah A. Marquez-Curtis,&nbsp;Janet A.W. Elliott","doi":"10.1016/j.cryobiol.2025.105368","DOIUrl":"10.1016/j.cryobiol.2025.105368","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105368"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Daily changes in goat sperm quality, cryoresistance, and AQP3 relocation","authors":"Esther Alba ,&nbsp;Adolfo Toledano-Díaz ,&nbsp;Cristina Castaño ,&nbsp;Rosario Velázquez ,&nbsp;Belén Martínez-Madrid ,&nbsp;Julian Santiago-Moreno","doi":"10.1016/j.cryobiol.2025.105388","DOIUrl":"10.1016/j.cryobiol.2025.105388","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105388"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiofrequency electromagnetic heating enhanced vitrification of viable large cartilage tissues","authors":"Haiyang Ma ,&nbsp;Josh Wewerk ,&nbsp;Ge Pan ,&nbsp;A.J. Riffe ,&nbsp;Peng Chen ,&nbsp;Yaqian Sun ,&nbsp;John M. Alford ,&nbsp;Kristi Helke ,&nbsp;Girish Srinivas ,&nbsp;Hai Yao ,&nbsp;David P. Eisenberg ,&nbsp;Shangping Wang","doi":"10.1016/j.cryobiol.2025.105385","DOIUrl":"10.1016/j.cryobiol.2025.105385","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105385"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organ donor bone marrow banking: A new paradigm","authors":"Erik J. Woods PhD, HCLD","doi":"10.1016/j.cryobiol.2025.105408","DOIUrl":"10.1016/j.cryobiol.2025.105408","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105408"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rebuilding the ovary: Innovative bioengineering approaches for fertility preservation","authors":"Christiani A. Amorim","doi":"10.1016/j.cryobiol.2025.105354","DOIUrl":"10.1016/j.cryobiol.2025.105354","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105354"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ice formation and its elmination in cryopreservation of bovine oocytes and embryos","authors":"Abdallah W. Abdelhady ,&nbsp;David W. Mittan-Moreau ,&nbsp;Patrick L. Crane ,&nbsp;Jingzhi Zhang ,&nbsp;Chia-Kuan Wu ,&nbsp;Yoke Lee Lee ,&nbsp;Meihong Shi ,&nbsp;Mariana Diel de Amorim ,&nbsp;Ian R. Porter ,&nbsp;Jingyue (Ellie) Duan ,&nbsp;Soon Hon Cheong ,&nbsp;Robert E. Thorne","doi":"10.1016/j.cryobiol.2025.105383","DOIUrl":"10.1016/j.cryobiol.2025.105383","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105383"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}