Cryobiology最新文献

{"title":"Permeable and non-permeable cryoprotectant-free cryopreservation of larger volumes human semen","authors":"Karekin D. Esmeryan , Todor A. Chaushev","doi":"10.1016/j.cryobiol.2025.105359","DOIUrl":"10.1016/j.cryobiol.2025.105359","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105359"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oocyte and embryo vitrification using automated equipment based on microfluidic chip","authors":"Jing Shen , Yukun Cao , Weijie Li , Xinli Zhou","doi":"10.1016/j.cryobiol.2025.105360","DOIUrl":"10.1016/j.cryobiol.2025.105360","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105360"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Granulosa cell-derived extracellular vesicles modulate oxidative stress in vitrified bovine oocytes","authors":"Judith Diaz-Muñoz , Sonia Gago , Yulia N. Cajas , Karina Cañón-Beltrán , Manel López-Béjar , Dimitrios Rizos , Teresa Mogas","doi":"10.1016/j.cryobiol.2025.105374","DOIUrl":"10.1016/j.cryobiol.2025.105374","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105374"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Numerical simulation of bioheat transfer during bronchial cryobiopsy using cryoprobes of different diameters","authors":"Junhong Tang ,&nbsp;Yudong Bao ,&nbsp;Wenqing Du ,&nbsp;Wen Wei","doi":"10.1016/j.cryobiol.2025.105320","DOIUrl":"10.1016/j.cryobiol.2025.105320","url":null,"abstract":"<div><div>Transbronchial cryobiopsy using flexible cryoprobes is an emerging biopsy technique. However, an inappropriately sized cryoprobe or inaccurate freezing time may result in tissue cryoinjury or substandard tissue specimens. This study uses numerical simulation to explore the effects of different diameter cryoprobes (1.1 mm, 1.9 mm, 2.4 mm) on tissue temperature distribution and phase transition in bronchial tumor cryobiopsy. A three-dimensional bronchial model containing tumors is constructed. The heat transfer process of the cryoprobes within 0–10 s at different insertion depths (0–1.0 mm, interval 0.1 mm) is simulated based on Pennes bioheat equation and the effective heat capacity method. Multi-physics effects are analyzed by coupling respiratory airflow. The results demonstrate that increasing the cryoprobe diameter and its insertion depth leads to an expansion of the low-temperature zone within the tissue, thereby elevating the risk of cryoinjury to surrounding peritumoral tissues. Moreover, the effect of cryoprobe diameter on tissue phase transition is more significant than that of insertion depth. Increasing the cryoprobe diameter will reduce the distance to adjacent peritumoral tissues, resulting in rapid expansion of the frozen region within peritumoral tissues. In contrast, the insertion depth primarily influences the axial extension of the frozen region. Additionally, respiratory airflow demonstrates no significant impact on temperature distribution. This study provides a theoretical foundation for the clinical selection of cryoprobe parameters and optimization of freezing duration, facilitating the acquisition of sufficient tissue samples while minimizing cryoinjury to healthy tissues.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105320"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mechanism of optimized two-step osmo-protection in regulating the cell homeostasis of ‘Light Yellow’ Petunia × Calibrachoa callus","authors":"Yuanshan Zhang ,&nbsp;Guanqun Chen ,&nbsp;Junsong Pan ,&nbsp;Xiaohui Shen","doi":"10.1016/j.cryobiol.2025.105332","DOIUrl":"10.1016/j.cryobiol.2025.105332","url":null,"abstract":"<div><div>Cryopreservation is essential for long-term plant germplasm preservation, yet the mechanisms underlying cellular responses during the critical dehydration phase remain poorly understood. In this study, callus tissues of ‘Light Yellow’ <em>Petunia</em> × <em>Calibrachoa</em> were used to compare the cytological, physiological, biochemical, and molecular effects of one-step (T1) and two-step (T2-1, T2-2) osmo-protection. The one-step method (T1) directly exposed tissues to MS medium with 2 M glycerol and 0.35 M sucrose for 30 min, while the two-step strategy involved 15 min in glycerol alone (T2-1) followed by 15 min in glycerol-sucrose (T2-2). The two-step method markedly improved cell viability, reaching 90.15 % after 30 min, compared with 12.32 % in T1. LF-NMR revealed that two-step treatment reduced total water content (71.35 %) and free water proportion (52.6 %), while increasing bound water (7.26 %), accompanied by upregulation of aquaporin genes (<em>TIP</em>, <em>PIP</em>), indicating enhanced water regulation. After 30 min, cell shrinkage reached 69.65 % in T1 but only 49.59 % in the two-step treatment, suggesting a more moderate dehydration rate. Transmission electron microscope (TEM) confirmed that two-step treatment could protect the integrity of cell membranes, vacuoles, and organelles, while T1 caused plasmolysis, membrane collapse, and mitochondrial damage. Biochemical assays showed reduced malondialdehyde (MDA) and conductivity in two-step treatments, alleviating lipid peroxidation and permeability change. Analysis of cell wall by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS) revealed that T2-2 treatments not only promoted accumulation of hemicellulose (54.52 mg/g) and pectin (303.82 μmol/g) for enhancing wall strength, but also facilitated the accumulation of osmoprotective sugars, such as glucose (171.42 μg/mL), fructose (25 μg/mL), and xylose (13.76 μg/mL). Quantitative reverse transcription polymerase chain reaction (qRT-PCR) results further elucidated alongside upregulation expression of sugar metabolism-related the genes (<em>INV</em>, <em>HK</em>). Antioxidant enzymes (<em>CAT</em>, <em>POD</em>) and wall remodeling genes (<em>XTH</em>, <em>PMEI</em>) were also regulated during two-step treatment, suggesting multiple pathways mediate osmotic stress responses. In conclusion, the two-step osmo-protection effectively mitigates dehydration stress, preserves viability and structural integrity, and activates molecular defense mechanisms, demonstrating clear advantages for cryopreservation of high-moisture, freeze-sensitive plant materials.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105332"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145387557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of B cell medicine cryopreservation process","authors":"Mark Lalli,&nbsp;Jordan Smelker,&nbsp;Rashmi Kshirsagar","doi":"10.1016/j.cryobiol.2025.105366","DOIUrl":"10.1016/j.cryobiol.2025.105366","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105366"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145619911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving non-contact cooling rate with liquid nitrogen by hierarchical surface structures","authors":"Meng Shi","doi":"10.1016/j.cryobiol.2025.105371","DOIUrl":"10.1016/j.cryobiol.2025.105371","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105371"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid metabolism homeostasis and low-level glycogen metabolism in the Harderian gland of hibernating Daurian ground squirrels","authors":"Lu-Fan Li,&nbsp;Yu-Jing Yan,&nbsp;Yi-Man Liu,&nbsp;Qing-Ying Wang,&nbsp;Ming-Di Wang,&nbsp;Jin-Hui Xu,&nbsp;Zhe Wang","doi":"10.1016/j.cryobiol.2025.105336","DOIUrl":"10.1016/j.cryobiol.2025.105336","url":null,"abstract":"<div><div>The Daurian ground squirrel (<em>Spermophilus dauricus</em>) is a mammalian hibernator that undergoes prolonged torpor bouts in winter, periodically interrupted by spontaneous arousals. However, whether the function of the Harderian gland (HG)—an orbital endocrine organ—and its associated glycogen and lipid metabolic activity vary across different physiological states of hibernation remains unclear. In this study, we conducted quantitative analyses of HG morphology and assessed glycogen and lipid metabolism in Daurian ground squirrels across distinct hibernation phases. Results showed that: (1) Compared with the pre-hibernation (PRE) period, HG glycogen content declined during torpor, while elevated glycogen synthase (GS) activity during inter-bout arousal (IBA) restored glycogen levels. (2) Triacylglycerols (TAG) and free fatty acid (FA) concentrations in the HG remained stable and higher than PRE levels throughout hibernation. Notably, fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC) activities peaked during torpor, while lipase (LPS) activity was highest during IBA. These findings suggest that lipid accumulation in the HG is regulated through a dynamic balance of synthesis and degradation, with lipid metabolism predominating as the main energy strategy during hibernation. In contrast, glycogen metabolism remains suppressed during torpor, primarily regulated by GS activity.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105336"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-throughput evaluation of cryoprotective agents for mixture effects that reduce toxicity","authors":"Nima Ahmadkhani ,&nbsp;Cameron Sugden ,&nbsp;James D. Benson ,&nbsp;Ali Eroglu ,&nbsp;Adam Z. Higgins","doi":"10.1016/j.cryobiol.2025.105316","DOIUrl":"10.1016/j.cryobiol.2025.105316","url":null,"abstract":"<div><div>Vitrification is a promising approach for cryopreserving complex biological structures such as organs. However, to prevent ice formation, high concentrations of cell-permeable cryoprotective agents (CPAs) are required, which can be highly toxic. The current reliance on a small number of CPAs limits optimization of low-toxicity compositions for vitrification. To address this, there is growing interest in uncovering novel chemicals with analogous protective qualities. This may not only enhance vitrification efficacy but also mitigate toxic effects. In the current study, we employed a high throughput method to assess the toxicity of 21 compounds at room temperature, both individually and in binary combinations, using bovine pulmonary artery endothelial cells (BPAEC) as a model system. Our analysis revealed that toxicity increases with both exposure duration and concentration, and that several CPA combinations result in reduced overall toxicity. Notably, among all tested mixtures, four binary combinations—formamide/glycerol, dimethyl sulfoxide/1,3-propanediol, 1,2-propanediol/diethylene glycol, and 1,3-propanediol/diethylene glycol—produced a statistically significant decrease in toxicity, resulting in significantly higher viability for the 6 mol/kg mixture than both corresponding 6 mol/kg single CPA solutions. The high-throughput approach presented here will aid in building a comprehensive CPA toxicity database, which will improve our understanding of toxicity mechanisms and support the development of predictive models for identifying novel CPA mixtures with low toxicity.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105316"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ice-crystal formation during cooling and rewarming of concentrated sucrose solutions","authors":"Norihito Kimizuka","doi":"10.1016/j.cryobiol.2025.105301","DOIUrl":"10.1016/j.cryobiol.2025.105301","url":null,"abstract":"<div><div>The concentration dependence of ice-crystal formation during cooling and rewarming of sucrose solutions (51–72 wt%) was studied by differential scanning calorimetry and optical microscopy at cooling and heating rates of ±1.0–±3.0 °C/min. During cooling, ice crystals formed in solutions with concentrations of up to 60 wt%, but were not found in solutions with concentrations of ≥61 wt%. In contrast, ice-crystal at solution concentrations of 59–62 wt% during rewarming was irregular and accompanied by devitrification (<em>T</em>_d) and/or recrystallization (<em>T</em>_r). However, at ≥64 wt%, only devitrification occurred, and no ice crystals were formed at 72 wt% or higher. These results suggest that the <em>T</em>_d curve on the binary system state diagram exists at concentrations of ≥60 wt%, with concentrations of ≥64 wt% resulting in ice-crystal formation only via devitrification at −40 to −30 °C during rewarming at 3.0 °C/min.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105301"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}