Cryobiology最新文献_第6页

Glucose and maltose supplementation in the freezing medium of Creole rooster spermatozoa improves in vivo fertility 在克里奥尔公鸡精子冷冻培养基中添加葡萄糖和麦芽糖可提高体内育性

IF 2.1 3区生物学

Cryobiology Pub Date : 2026-03-01 Epub Date: 2025-12-19 DOI: 10.1016/j.cryobiol.2025.105573

Elías Segarra Zenteno , Liliana Morocho , Karen Vásquez , Mauricio Duma , Diego A. Galarza

{"title":"Glucose and maltose supplementation in the freezing medium of Creole rooster spermatozoa improves in vivo fertility","authors":"Elías Segarra Zenteno , Liliana Morocho , Karen Vásquez , Mauricio Duma , Diego A. Galarza","doi":"10.1016/j.cryobiol.2025.105573","DOIUrl":"10.1016/j.cryobiol.2025.105573","url":null,"abstract":"<div><div>This study investigated the effects of glucose-maltose supplementation in freezing medium on the kinematic, viability, and fertility of Creole rooster spermatozoa. Fifty ejaculates from 10 Creole roosters were obtained in 5 weekly sessions to create 10 heterologous pools. Each pool was extended with Lake-Ravie plus 6 % dimethylacetamide and allocated to: GM1 (0.64 % glucose + 0.33 % maltose), GM2 (0.72 % glucose + 0.17 % maltose), or non-supplemented control. After thawing, the control presented higher motility and beat-cross frequency than GM1 and GM2 (P < 0.05). Viability was greater in GM1 and control than in GM2 (P < 0.05). Crucially, the fertility after artificial insemination of hens with frozen-thawed sperm from the GM1 group was higher (P < 0.05) compared to the non-supplemented group. In conclusion, glucose-maltose supplementation to the freezing medium can improve the viability and fertility of frozen-thawed Creole rooster semen, supporting its use in genetic conservation and breeding programs.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"122 ","pages":"Article 105573"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A brief overview of the development of oocyte and embryo cryopreservation strategies with a focus on the roles of sugars 简要概述了卵母细胞和胚胎冷冻保存策略的发展，重点介绍了糖的作用。

IF 2.1 3区生物学

Cryobiology Pub Date : 2026-03-01 Epub Date: 2025-12-30 DOI: 10.1016/j.cryobiol.2025.105577

Krzysztof Papis

引用次数: 0

Can a tropical lizard (Xenosaurus platyceps) survive freezing temperatures? 热带蜥蜴（platyceps Xenosaurus）能在严寒中生存吗？

IF 2.1 3区生物学

Cryobiology Pub Date : 2026-03-01 Epub Date: 2026-02-10 DOI: 10.1016/j.cryobiol.2026.105595

Natalia Fierro-Estrada , Fausto R. Méndez-de la Cruz , Oswaldo Téllez-Valdes

{"title":"Can a tropical lizard (Xenosaurus platyceps) survive freezing temperatures?","authors":"Natalia Fierro-Estrada , Fausto R. Méndez-de la Cruz , Oswaldo Téllez-Valdes","doi":"10.1016/j.cryobiol.2026.105595","DOIUrl":"10.1016/j.cryobiol.2026.105595","url":null,"abstract":"<div><div>Thermoregulation is crucial for lizards, which can employ stenothermic or eurythermic strategies and active or passive thermoregulation. Some organisms that live at low temperatures use physiological freeze avoidance (supercooling) or freeze tolerance mechanisms, often involving glucose as a cryoprotectant. Lizards from the ancient family Xenosauridae are typically thermoconformers, with some species exhibiting remarkably low body temperatures. This study investigated the thermal ecology of <em>Xenosaurus platyceps</em> in a low-elevation population (390 m) throughout the year. We examined thermoregulatory behaviour, cold tolerance, and blood glucose maintenance, as well as seasonal variation in thermoregulation patterns, supercooling points, and glucose concentrations in this tropical species. This study demonstrates that the micro-endemic lizard <em>Xenosaurus platyceps</em> can survive experimental freezing, marking the first such report for the Xenosauridae. Body temperature correlated significantly with environmental temperature, particularly with substrate temperature. The lizards showed stenothermic behaviour with body temperatures ranging from 20.8 to 30.8 °C, with significant differences amongst seasons. The species actively thermoregulated during spring and winter, exhibited thermoconformer behaviour during autumn, and avoided extremely warm microhabitats during summer. Remarkably, lizards had crystallisation points as low as −7.01 °C during spring, with a moderate increase in blood glucose after freezing that likely contributed to freeze tolerance. The study demonstrates that <em>X. platyceps</em> has remarkable seasonal thermal adaptability and resistance to sub-zero temperatures.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"122 ","pages":"Article 105595"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146164613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of FBS-DMSO cryoprotectant composition in artificial microbiome models mimicking key gut microbiota enterotypes FBS-DMSO冷冻保护剂组合物在模拟关键肠道微生物群肠型的人工微生物组模型中的有效性

IF 2.1 3区生物学

Cryobiology Pub Date : 2026-03-01 Epub Date: 2026-01-13 DOI: 10.1016/j.cryobiol.2026.105582

L.V. Zalomova, E.E. Fesenko Jr.

{"title":"Effectiveness of FBS-DMSO cryoprotectant composition in artificial microbiome models mimicking key gut microbiota enterotypes","authors":"L.V. Zalomova, E.E. Fesenko Jr.","doi":"10.1016/j.cryobiol.2026.105582","DOIUrl":"10.1016/j.cryobiol.2026.105582","url":null,"abstract":"<div><div>The ratio of microorganisms in the composition of the microflora of the small and large intestines plays a crucial role in human health. Therefore, it is essential to preserve the original proportions of species over an extended period for their further therapeutic application. It has previously been established that three primary enterotypes dominate the human gut microbiota: <em>Bacteroides</em>, <em>Prevotella</em>, and <em>Ruminococcus</em>. However, there is no precise information on how their species ratios are affected by deep freezing. In our study, we examined the preservation of the ratios of microorganisms in the human gut before and after cryopreservation, represented as distinct clusters consisting of four different bacterial species dominant in the gut microbiome. Using photometric registration of optical density and fluorescent staining methods, we demonstrated that the viability of most bacteria remained high in the cryoprotective medium of 5 % Me<sub>2</sub>SO/FBS. Additionally, the calculation of the Pattern Comparison Index (PCI) showed good results in maintaining the community structure of bacteria in each of the artificial models. Thus, this modeling of microbiocenoses allows for the identification of patterns in the preservation of their quantitative composition during long-term storage in liquid nitrogen.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"122 ","pages":"Article 105582"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultra-rapid freezing yields a higher cryoresistance than the conventional slow freezing of epididymal guinea pig (Cavia porcellus) spermatozoa 超高速冷冻比常规慢速冷冻对附睾豚鼠精子具有更高的抗冻性。

IF 2.1 3区生物学

Cryobiology Pub Date : 2026-03-01 Epub Date: 2026-02-18 DOI: 10.1016/j.cryobiol.2026.105596

Diego A. Galarza , Christian A. Hernández-Lojano , Anddy J. Salinas-Ulloa , Mauricio Duma , Manuel E. Soria , Gonzalo Castro-Jerez , Juan Pablo Garzón

{"title":"Ultra-rapid freezing yields a higher cryoresistance than the conventional slow freezing of epididymal guinea pig (Cavia porcellus) spermatozoa","authors":"Diego A. Galarza , Christian A. Hernández-Lojano , Anddy J. Salinas-Ulloa , Mauricio Duma , Manuel E. Soria , Gonzalo Castro-Jerez , Juan Pablo Garzón","doi":"10.1016/j.cryobiol.2026.105596","DOIUrl":"10.1016/j.cryobiol.2026.105596","url":null,"abstract":"<div><div>Ultra-rapid (UR) freezing represents a promising alternative to conventional-slow (CS) freezing of spermatozoa in certain species, with the potential to reduce post-thaw cryodamage. This study evaluated the quality of guinea pig (<em>Cavia porcellus</em>) epididymal spermatozoa cryopreserved by either CS or UR freezing. Twenty sexually mature, clinically healthy males were subjected to bilateral orchiectomy. Epididymal sperm samples recovered by retrograde flushing from the right testes were conventionally frozen by exposing them to liquid nitrogen (LN<sub>2</sub>) vapors, while samples from the left testes were ultra-rapidly frozen by direct immersion of 20 μl droplets into LN<sub>2</sub>. The TCG-EY medium (tris, citric acid, glucose, and 10% egg yolk, 1% BSA) was supplemented with 3% glycerol and 3% dimethylformamide for the CS freezing method, whereas 100 mM sucrose was added for the UR freezing procedure. Both methods drastically reduced the kinematic variables and membrane integrity after cryopreservation (P < 0.01). However, the total and progressive motilities, curvilinear and straight-line velocities with rapid progression, and lateral head displacement were significantly higher in the UR group than in the CS group (P < 0.05). Moreover, sperm viability and acrosomal and DNA integrity were higher after UR freezing than after CS freezing (P < 0.05). In conclusion, ultra-rapid freezing provided better preservation of sperm motility, membrane function, and DNA integrity than conventional slow freezing, indicating greater cryoresistance in guinea pig epididymal spermatozoa. These findings represent a valuable advancement for the cryopreservation of sperm in this species.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"122 ","pages":"Article 105596"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146225876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Need for harmonized terminology in cryopreservation to support reproducibility, regulation, and translation 需要在低温保存中统一术语以支持可重复性、规范性和翻译

IF 2.1 3区生物学

Cryobiology Pub Date : 2026-03-01 Epub Date: 2026-02-13 DOI: 10.1016/j.cryobiol.2026.105584

Lakshya Gangwar , Lowell Wolfe , Nikolas Zuchowicz , Irina Filz von Reiterdank , Srivasupradha Ramesh , Bat-Erdene Namsrai , Joseph Kangas , Joseph Sushil Rao , Matthew Powell-Palm , Timothy L. Pruett , John C. Bischof , Susan M. Wolf , Korkut Uygun

{"title":"Need for harmonized terminology in cryopreservation to support reproducibility, regulation, and translation","authors":"Lakshya Gangwar , Lowell Wolfe , Nikolas Zuchowicz , Irina Filz von Reiterdank , Srivasupradha Ramesh , Bat-Erdene Namsrai , Joseph Kangas , Joseph Sushil Rao , Matthew Powell-Palm , Timothy L. Pruett , John C. Bischof , Susan M. Wolf , Korkut Uygun","doi":"10.1016/j.cryobiol.2026.105584","DOIUrl":"10.1016/j.cryobiol.2026.105584","url":null,"abstract":"<div><div>As cryopreservation technologies continue to develop, the need for harmonized terminology across the multitude of disciplines where cryopreservation is applied is becoming increasingly acute. Terminology in cryopreservation remains inconsistent, leading to confusion and barriers to progress. Applications of cryopreservation in medicine, food, agriculture, and conservation remain limited by this lack of consensus. Inconsistent terminology contributes to ethical, legal, and societal issues in translating and integrating new cryopreservation technologies. Here we identify the problem with examples of cryopreservation terminology that demand harmonization. We describe the need for terminological consistency by providing examples of effective terminology harmonization projects in related fields. We propose next steps, highlighting the important role that professional societies should play to reach consensus on terminology among cryopreservation stakeholders and the broader cryobiology community.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"122 ","pages":"Article 105584"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146170164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 2.1 3区生物学

Cryobiology Pub Date : 2026-01-01

引用次数: 0

Cryotherapy reduces functional impairment and oxidative damage after sciatic nerve injury in rats 冷冻疗法可减轻大鼠坐骨神经损伤后的功能损伤和氧化损伤。

IF 2.1 3区生物学

Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-11-02 DOI: 10.1016/j.cryobiol.2025.105342

Jaíne Dalmolin , Andrezza B.V. Furtado , Diane D. Hartmann , Rodrigo P. Martins , Magale D. Furquim , Felix A. Soares , Luis Ulisses Signori , Gustavo O. Puntel

{"title":"Cryotherapy reduces functional impairment and oxidative damage after sciatic nerve injury in rats","authors":"Jaíne Dalmolin , Andrezza B.V. Furtado , Diane D. Hartmann , Rodrigo P. Martins , Magale D. Furquim , Felix A. Soares , Luis Ulisses Signori , Gustavo O. Puntel","doi":"10.1016/j.cryobiol.2025.105342","DOIUrl":"10.1016/j.cryobiol.2025.105342","url":null,"abstract":"<div><div>Peripheral Nerve Injury (PNI) has a multifactorial etiology and results in functional impairment in both motor and sensory behavior, which can be reversed by appropriate treatment strategies. As PNI results in an inflammatory response, conservative modalities with antioxidant potential, such as cryotherapy, are promising tools to be studied. The aim of this study was to analyze the effects of cold water immersion on functional, oxidative, and morphological changes after a PNI model in rats. PNI was surgically induced by compression of the sciatic nerve, and cryotherapy started the day after the injury and was applied daily for 15 days. PNI caused a significant impairment in motor behavior that was accompanied by microscopic damage to the structure of the sciatic nerve. The observed functional and morphological changes were accompanied by a significant oxidative damage in the skeletal muscle innervated by the injured sciatic nerve. The effects of cryotherapy in reducing motor impairment were corroborated by the modulation of morphological damage to the sciatic nerve and also by the reduction of oxidative damage in innervated areas. Taken together, the results presented suggest that cryotherapy may be a potential treatment resource after a PNI; however, more studies are needed to clarify the mechanisms underlying the origin of its therapeutic effects.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105342"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145437579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of co-supplementation of trehalose with fetuin and taxifolin in freezing extender on quality parameters of bull spermatozoa following freeze-thaw process 冻融剂中海藻糖与胎儿素和紫杉素共同添加对公牛精子冻融过程中质量参数的影响。

IF 2.1 3区生物学

Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-10-02 DOI: 10.1016/j.cryobiol.2025.105330

Mustafa Numan Bucak , Morteza Taravat , Oya Korkmaz , Ali Soliemanzadeh , Mojtaba Karimpour , Mohammad Saber Maroufi , Neda Abedpour , Sadık Küçükgünay , Tohid Rezaei Topraggaleh

{"title":"Effects of co-supplementation of trehalose with fetuin and taxifolin in freezing extender on quality parameters of bull spermatozoa following freeze-thaw process","authors":"Mustafa Numan Bucak , Morteza Taravat , Oya Korkmaz , Ali Soliemanzadeh , Mojtaba Karimpour , Mohammad Saber Maroufi , Neda Abedpour , Sadık Küçükgünay , Tohid Rezaei Topraggaleh","doi":"10.1016/j.cryobiol.2025.105330","DOIUrl":"10.1016/j.cryobiol.2025.105330","url":null,"abstract":"<div><div>Glycerol is commonly used as a cryoprotectant in bovine sperm cryopreservation; however, its cytotoxic effects can negatively impact sperm quality. This study aimed to reduce glycerol concentration in semen extenders by supplementing with natural antioxidants (taxifolin and fetuin) and the disaccharide trehalose. Twelve ejaculates were collected from three bulls and initially evaluated, then diluted in a tris-egg yolk (TEY) extender containing either 7 % or 4 % glycerol. The control group received 7 % glycerol (TEY), while experimental groups included G7F (TEY + fetuin), G7T (TEY + taxifolin), G7M (TEY + fetuin + taxifolin), and 4 % glycerol groups supplemented with 50 mM trehalose: G4 (TEY + trehalose), G4F (TEY + fetuin), G4T (TEY + taxifolin), and G4M (TEY + fetuin + taxifolin). Post-thaw evaluations included assessments of sperm motility, plasma membrane integrity, acrosome integrity, mitochondrial membrane potential (MMP), DNA fragmentation, and oxidative stress markers. The G4F and G4T groups exhibited significantly higher total and progressive motility, viability, plasma membrane integrity, and MMP compared to other groups (p < 0.05). Furthermore, these groups showed significantly lower malondialdehyde (MDA) levels, indicating reduced lipid peroxidation and oxidative stress (p < 0.05). In conclusion, co-supplementation of 50 mM trehalose with 50 μM taxifolin and 5 mg/mL fetuin in a cryopreservation extender containing 4 % glycerol may enhance the post-thaw quality of bull spermatozoa.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"121 ","pages":"Article 105330"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HIFU enables structural preservation in cryopreserved vascular tissue: Insights from electron microscopy HIFU能够在冷冻保存的血管组织中进行结构保存：来自电子显微镜的见解

IF 2.1 3区生物学

Cryobiology Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105395

Enrique Alcalá , Virginia Armas , Laura Encabo , Carmen Leiva , Juan José Jiménez , Emil Kendziorr , Ramón Risco

引用次数: 0