Cryobiology最新文献_第6页

An efficient droplet-vitrification cryopreservation procedure for high imperatorin-yielding hairy root clones of Urena lobata 白莲毛状根高产量无性系的高效液滴玻璃化冷冻保存方法。

IF 2.3 3区生物学

Cryobiology Pub Date : 2024-12-04 DOI: 10.1016/j.cryobiol.2024.105186

Dai Minh Cao , Anh Lan Bui , Le Van Bui , Phuong Ngo Diem Quach

{"title":"An efficient droplet-vitrification cryopreservation procedure for high imperatorin-yielding hairy root clones of Urena lobata","authors":"Dai Minh Cao , Anh Lan Bui , Le Van Bui , Phuong Ngo Diem Quach","doi":"10.1016/j.cryobiol.2024.105186","DOIUrl":"10.1016/j.cryobiol.2024.105186","url":null,"abstract":"<div><div>The valuable anti-cancer and anti-inflammatory secondary metabolite, imperatorin, has been found in the hairy roots (HRs) of <em>Urena lobata</em>. However, an increasing number of problems related to cryo-injury and cryoprotectant toxicity could potentially reduce the quality of root clones, highlighting the need to develop a reliable technique for long-term preservation. Based on the impact of the successive steps of the initial droplet-vitrification procedure employed for cryopreservation of HRs using the histological evaluation of plasmolysis, various selected factors were independently investigated. The maximum plasmolysis was observed after the preculture with 0.5 M sucrose (46.59 %) and the dehydration treatment (48.32 %). In the improved cryopreservation procedure, when prolonged preculture for 72 h in liquid WPM with 0.3 M sucrose and dehydration in the appropriate vitrification solution, which included 30 % (w/v) glycerol and 50 % (w/v) sucrose, for 10 min at 0 °C, the plasmolysis in the two steps was significantly reduced in comparison with the untreated control. The cryopreserved HRs could increase their regeneration to 93.3 % and regenerated root length to 4.65 cm. Their growth and imperatorin production were almost the same as those of untreated controls after three to five subsequent subcultures. Our results have demonstrated that our new approach, which only focuses on the key factors that significantly increase the plasmolysis, modifies their level to fit with the HR cells of <em>U. lobata</em>. The early application of the plasmolysis evaluation method may immediately screen the impact of factors on individual root cells instead of spending time and cost evaluating the recovery of root tips at each step to develop an efficient cryopreservation procedure.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"118 ","pages":"Article 105186"},"PeriodicalIF":2.3,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

No synergistic effect of extracellular cryoprotectants with dimethyl sulfoxide in the conservation of northern tiger cat fibroblasts 细胞外低温保护剂与二甲基亚砜在保护北方虎猫成纤维细胞方面没有协同作用。

IF 2.3 3区生物学

Cryobiology Pub Date : 2024-12-03 DOI: 10.1016/j.cryobiol.2024.105169

João Vitor da Silva Viana , Lhara Ricarliany Medeiros de Oliveira , Luanna Lorenna Vieira Rodrigues , Yasmin Beatriz França Moura , Antonia Beatriz Mendonça Pereira , Patrícia Vasconcelos Alves , Herlon Victor Rodrigues Silva , Alexsandra Fernandes Pereira

{"title":"No synergistic effect of extracellular cryoprotectants with dimethyl sulfoxide in the conservation of northern tiger cat fibroblasts","authors":"João Vitor da Silva Viana , Lhara Ricarliany Medeiros de Oliveira , Luanna Lorenna Vieira Rodrigues , Yasmin Beatriz França Moura , Antonia Beatriz Mendonça Pereira , Patrícia Vasconcelos Alves , Herlon Victor Rodrigues Silva , Alexsandra Fernandes Pereira","doi":"10.1016/j.cryobiol.2024.105169","DOIUrl":"10.1016/j.cryobiol.2024.105169","url":null,"abstract":"<div><div>The success of somatic cell cryobanks is dependent on establishing reproducible cryopreservation methodologies. We supposed that associated extracellular cryoprotectants (sucrose and L-proline) with 2.5 or 10 % dimethyl sulfoxide (Me<sub>2</sub>SO) could guarantee better northern tiger cat cells quality rates after thawing when compared to Me<sub>2</sub>SO alone. Therefore, we evaluated the effects of sucrose or L-proline with 2.5 or 10 % Me<sub>2</sub>SO on the cryopreservation of northern tiger cat fibroblasts. Somatic cells were also cryopreserved with 2.5 % or 10 % Me<sub>2</sub>SO alone. All cells were analyzed for morphology, membrane integrity, proliferative activity, metabolism, apoptosis classification, reactive oxygen species (ROS) levels, and mitochondrial membrane potential (ΔΨm). Regardless of the cryoprotective solution, cryopreservation did not affect morphology, membrane integrity after culture, proliferative activity, and metabolism (P > 0.05). However, immediately after thawing, 2.5 % Me<sub>2</sub>SO with L-proline and 10 % Me<sub>2</sub>SO promoted higher rates of membrane integrity when compared to the other cryopreserved groups (P < 0.05). Interestingly, cells cryopreserved with 10 % Me<sub>2</sub>SO maintained ROS levels similar to non-cryopreserved cells (P > 0.05). However, the percentage of viable cells evaluated by apoptosis classification was reduced when using 10 % Me<sub>2</sub>SO with L-proline compared to non-cryopreserved groups (P < 0.05). Additionally, ΔΨm was altered in all cryopreserved groups (P < 0.05). In summary, sucrose and L-proline were less effective in cryopreservation of northern tiger cat fibroblasts in the presence of 2.5 % or 10 % Me<sub>2</sub>SO. Also, 10 % Me<sub>2</sub>SO appears to be the most suitable cryoprotectant for the formation of cryobanks of this species.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"118 ","pages":"Article 105169"},"PeriodicalIF":2.3,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryo-conservation of temperate berry crops at ICAR-NBPGR 温带浆果作物在ICAR-NBPGR的低温保存

IF 2.3 3区生物学

Cryobiology Pub Date : 2024-12-01 DOI: 10.1016/j.cryobiol.2024.105034

Sandhya Gupta , Narender Negi , Sheikh M. Sultan

引用次数: 0

Cryopreservation with ice nucleators enables high-throughput screening of T-Cells 冰核冷冻保存使t细胞的高通量筛选成为可能

IF 2.3 3区生物学

Cryobiology Pub Date : 2024-12-01 DOI: 10.1016/j.cryobiol.2024.104992

Natalia Gonzalez-Martinez , Matthew Gibson

引用次数: 0

Numerical analysis of cooling within a sealed container under high-pressure solid-surface conditions 高压固体表面条件下密封容器内冷却的数值分析