Cryobiology最新文献

{"title":"Expert consensus on cryosurgery therapy of mucosal melanoma on head and neck","authors":"Yadong Wu ,&nbsp;Guoxin Ren ,&nbsp;Moyi Sun ,&nbsp;Zhangui Tang ,&nbsp;Longjiang Li ,&nbsp;Jian Meng ,&nbsp;Zhijun Sun ,&nbsp;Shaoyan Liu ,&nbsp;Yue He ,&nbsp;Wei Shang ,&nbsp;Bo Li ,&nbsp;Jie Zhang ,&nbsp;Heming Wu ,&nbsp;Yi Li ,&nbsp;Shaohui Huang ,&nbsp;Jiong Lü ,&nbsp;Zhongcheng Gong ,&nbsp;Jun Wang ,&nbsp;Anxun Wang ,&nbsp;Zhiyong Li ,&nbsp;Wei Guo","doi":"10.1016/j.cryobiol.2025.105280","DOIUrl":"10.1016/j.cryobiol.2025.105280","url":null,"abstract":"<div><div>Cryoablation therapy for tumors has a long history of clinical application. Its anti-tumor mechanisms and histopathological changes have been well established, with extensive clinical practice demonstrating its safety and efficacy, theoretically making it an ideal modality for tumor treatment. Historically constrained by limitations in cryogenic media and freezing equipment, its therapeutic effectiveness and clinical adoption were significantly restricted. The emergence of new-generation cryoablation systems represented by Argon-Helium cryosurgical systems has achieved substantial advancements in refrigeration efficiency, ablation range precision, and temperature monitoring accuracy, thereby greatly promoting the widespread adoption of tumor cryoablation technology. This consensus systematically summarizes the mechanisms of cryoablation technology, indications for cryotherapy in head and neck mucosal melanoma, standardized clinical treatment protocols, management of adverse reactions, and related principles. It aims to provide authoritative references for standardizing cryoablation therapy in the treatment of head and neck mucosal melanoma.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105280"},"PeriodicalIF":2.3,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144611680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DFT-based evaluation of cryoprotectants and their role in lyophilization success","authors":"Mariia S. Ashikhmina ,&nbsp;Pavel V. Nesterov ,&nbsp;Vladislav S. Filozop ,&nbsp;Mikhail O. Volodarskiy ,&nbsp;Olga Y. Orlova ,&nbsp;Michael Nosonovsky ,&nbsp;Alexander S. Novikov ,&nbsp;Ekaterina V. Skorb","doi":"10.1016/j.cryobiol.2025.105282","DOIUrl":"10.1016/j.cryobiol.2025.105282","url":null,"abstract":"<div><div>Cryopreservation and lyophilization are essential for preserving and transporting biological samples, including bacterial cultures. These processes face severe challenges due to the formation of ice crystals that can damage cellular structures. Cryoprotectants (CPAs) are vital in reducing this damage by inhibiting ice nucleation and growth. The present study examines the use of various CPAs, focusing on their ability to enhance the survival of <em>Bacillus coagulans</em> and <em>Streptococcus thermophilus</em> during cryo-freezing and lyophilization. Using density functional theory (DFT) calculations, we analyzed interactions between water molecules. We selected CPAs (sucrose, fructosofuranose, glucose, fructose, and glycerol) to predict their effectiveness in forming protective hydrate shells around bacterial cells. Our results indicate that sucrose, which has a low Gibbs free energy of solvation at low temperatures, provides the most effective cryoprotection. Experimental validation showed that a 12 % sucrose solution improves bacterial survival after lyophilization. We also investigated the effect of different freezing protocols on bacterial survival. Initial freezing at −18 °C and subsequent storage at −80 °C were the optimal methods, maximizing cell survival. The findings contribute to a deeper understanding of cryopreservation and lyophilization processes, with potential applications in biotechnology and biomedical fields.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105282"},"PeriodicalIF":2.3,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144569739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of freezing properties of aqueous leaf homogenates from Hippophae rhamnoides","authors":"Vitalii Mutsenko ,&nbsp;Evgenii Rubalskii ,&nbsp;Elias Anastassopoulos ,&nbsp;Dimitris Zaragotas ,&nbsp;Sara Leal Marin ,&nbsp;Bulat Sydykov ,&nbsp;Ido Braslavsky ,&nbsp;Alexander Y. Petrenko ,&nbsp;Birgit Glasmacher ,&nbsp;Oleksandr Gryshkov","doi":"10.1016/j.cryobiol.2025.105281","DOIUrl":"10.1016/j.cryobiol.2025.105281","url":null,"abstract":"<div><div><em>Hippophae rhamnoides</em> is a cosmopolitan shrub that has attracted the attention of many scientists worldwide, not only for its therapeutic properties and metabolite richness but also for its ability to sustain freezing down to −40 °C. Endowed with the capacity to withstand harsh conditions, this plant controls freezing processes to cope with seasonal exposure to sub-zero temperatures. Intriguingly, <em>H. rhamnoides</em> is reported to harbour intrinsic ice-nucleating agents (INAs) active at temperatures above −5 °C. Moreover, its fruits provide a habitat for diverse populations of bacteria represented by genera that might exogenously initiate ice formation on vegetative and reproductive parts. Inspired by the multifaceted cold adaptation features <em>H. rhamnoides</em>, in this work, we have examined freezing patterns of its aqueous crude leaf homogenates (CLHs) in admixture with cryoprotective agents (CPAs) known to promote supercooling: antifreeze protein (AFP) type III, sucrose, trehalose, and dimethyl sulfoxide (Me₂SO). For this, we employed infrared thermography, cryomicroscopy, and differential scanning calorimetry (DSC). In order to identify the possible microbial contributions to <em>H. rhamnoides</em> INAs, bulk and filtered CLHs underwent microbiological studies. Analogous to Snomax™ (SM), the addition of CLH to disaccharides increased crystallization duration and reduced both the degree of supercooling and freezing time compared to the respective controls. Findings from experiments involving filtered and heat-treated CLHs suggest the presence of soluble ice nucleators that may originate from the plants themselves, as well as from ice-nucleating bacteria that are active at warmer sub-zero temperatures. Using conventional aerobic cultivation on chromogenic solid media, we obtained three bacterial isolates from bulk <em>H. rhamnoides</em> homogenates. Using 16S rRNA sequencing, the isolates were identified as representatives of the <em>Erwiniaceae</em> family. The sequence of one isolate clustered with those of <em>Pantoea agglomerans</em> (formerly known as <em>Enterobacter agglomerans</em> or <em>Erwinia herbicola</em>), a well-known bacterial ice-nucleating species. Interestingly, two isolates were clustered together with the recently delineated species <em>Duffyella gerundensis</em>. In summary, the investigation into the freezing characteristics of leaves derived from <em>H. rhamnoides</em> is of potential environmental and cryobiological utility.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105281"},"PeriodicalIF":2.3,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transplantation of vitrified osteochondral allografts revealed equivalent cartilage repair to fresh osteochondral allografts in a swine model","authors":"Mohammadhamed Shahsavari ,&nbsp;Kezhou Wu ,&nbsp;Adibehalsadat Ghazanfari ,&nbsp;Michael Doschak ,&nbsp;Michael Turner ,&nbsp;Mark Sommerfeldt ,&nbsp;Janet A.W. Elliott ,&nbsp;Nadr M. Jomha","doi":"10.1016/j.cryobiol.2025.105279","DOIUrl":"10.1016/j.cryobiol.2025.105279","url":null,"abstract":"<div><div>Despite improvements in treatment options and techniques, articular cartilage (AC) repair continues to be a challenge for orthopedic surgeons. The use of osteochondral allografts for AC repair is currently limited by the availability and short shelf life of donor cartilage. AC vitrification—which is an ice-free cryopreservation technology—will increase the availability of viable graft tissue for large joint defects. Although different protocols have been developed for AC vitrification, it is still unknown if vitrified and fresh osteochondral grafts are equivalent in terms of long-term joint resurfacing efficacy. In the present study, we compared the ability of vitrified porcine osteochondral dowels to repair full thickness cartilage defects to that of fresh porcine osteochondral dowels kept for 21 days at 4 °C. Eighteen pigs were randomly assigned to three experimental groups according to the kind of osteochondral dowel they received transplanted to medial femoral condyles: Long-Term Vitrified osteochondral dowel group (n = 6), Short-Term Vitrified osteochondral dowel group (n = 6), and Fresh osteochondral dowel group (n = 6). Lameness score was evaluated before and after transplantation surgery. Animals were euthanized 6 months after transplantation to obtain medial femoral condyles, and the contralateral condyle was used as the positive (no defect) control. Samples were evaluated for macroscopic appearance, morphometric-microstructural measures of bone, chondrocyte density, proteoglycan distribution, and repair tissue quality. The overall repair quality in all grafts (including fresh controls) was incomplete likely due to the animal model and surgical technique. In the areas where repair was documented, this study demonstrated that the repair effect of vitrified transplanted dowels (both Long-Term and Short-Term) was equivalent to that of the Fresh dowels that were stored for 21 days at 4 °C when comparing lameness scores, quality of regenerated cartilage based on direct visualization of the gross appearance (ICRS Scores), chondrocyte density, proteoglycan distribution within the extracellular matrix, morphometric measures of bone quantity and microstructural quality, and microscopic histological characteristics of the repair tissue. The vitrification of osteochondral dowels will mitigate the short shelf life of commonly used fresh osteochondral transplants, significantly increasing their availability for use by physicians and patients.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105279"},"PeriodicalIF":2.3,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144563617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized cryopreservation of embryogenic tissue of Picea abies based on differential scanning calorimetry","authors":"Jiwen Hu ,&nbsp;Ziyan Pu ,&nbsp;Chunhui Hao ,&nbsp;Huiling Yan ,&nbsp;Shumei Qian ,&nbsp;Tianqing Zhu ,&nbsp;Wenjun Ma ,&nbsp;Sanping An ,&nbsp;Lisheng Kong ,&nbsp;Junhui Wang","doi":"10.1016/j.cryobiol.2025.105278","DOIUrl":"10.1016/j.cryobiol.2025.105278","url":null,"abstract":"<div><div>Cryopreservation is the most economically, viable, and reliable method for long-term preservation of plant germplasm resources. Given the application of somatic embryogenesis techniques in conifer vegetative propagation, cryopreservation of Norway spruce (<em>Picea abies</em> (L.) Karst.) is highly necessary. Here, an optimal concentration of embryogenic tissue (ET) suspensions (0.1 g mL⁻¹) was achieved according to the regrowth rates of various Norway spruce lines and different solutions concentrations. On this basis, the Norway spruce ETs were cryopreserved using eight cryoprotectant pretreatments (CPTs). The enthalpy values of different CPTs were evaluated by differential scanning calorimetry (DSC). Subsequently, the relationship between the regrowth and the enthalpy was investigated. DSC showed that the enthalpy values of Norway spruce ETs treated with various CPTs significantly decreased during the warming. Moreover, the T6 (0.3 M sorbitol for 48 h, 10 %DMSO as the cryoprotectant) exhibited superior solution stability, as the regrowth rate of T6 was 2.94 times higher than that of T3. By comprehensive comparison, T6 might be considered an optimized CPT method for Norway spruce ETs. Notably, correlation analysis demonstrated that the enthalpy of warming stage and cooling stage was negatively correlated with the regrowth rate. Thus, the enthalpy of warming melting stage and cooling crystallization stage could serve as potential indicators of the regrowth rate. Our study provides an optimized CPT for Norway spruce ETs, which may offer a reference for the development of a novel rapid detection method to assess plant cryopreservation efficiency.</div></div><div><h3>Key message</h3><div>Optimized cryopreservation protocol based on differential scanning calorimetry.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105278"},"PeriodicalIF":2.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144536075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of temperature on acute kidney injury in a rat model of renal ischemia‒reperfusion","authors":"Jing Mei ,&nbsp;Minhui Xie,&nbsp;Congcong Dai,&nbsp;Yuhong Tang,&nbsp;Na Huang,&nbsp;Jun Ou","doi":"10.1016/j.cryobiol.2025.105276","DOIUrl":"10.1016/j.cryobiol.2025.105276","url":null,"abstract":"<div><div>Renal ischemia-reperfusion (I/R) injury is the main cause of acute kidney injury (AKI). A reliable and stable animal model is crucial for experimental research. Various factors affect the stability of animal models. In the present study, the effect of intraoperative temperature on the establishment of AKI model in rats, induced by I/R through bilateral renal pedicles clamping via the dorsal approach surgery, was investigated. Forty-eight male Sprague Dawley rats of 8–10 weeks were randomly divided into 6 groups (8 per group). The renal ischemia-reperfusion injury model was induced using a retroperitoneal double pedicle clamp procedure, using a clamping time of 50 min. The modeling procedure and sham operation were performed at different ambient air temperature, i.e., 15–20 °C, 20–25 °C and 25–30 °C. The general state of the rats, the ascites volume and mortality were observed after the operation. The serum creatinine (Scr) and urea nitrogen (BUN) levels of rats in each group were measured by an automatic biochemical analyzer 24 h post-operation. HE staining was used to observe pathological changes in renal tissues, and NF-κB p65 and interleukin-6 (IL-6) expression was measured by immunohistochemistry (IHC). TUNEL staining was used to assess apoptosis in renal tissues, and the expression of inflammatory markers, such as NF-κB and IL-6, and the apoptosis-related protein Bax was measured by Western blotting. There were no significant differences among the different sham operation groups. However, the abovementioned parameters were greater in the hypothermia, normothermia and hyperthermia model groups than in the corresponding sham operation groups, indicating successful model establishment. Scr and BUN levels were greater in the hyperthermia model group than in the other model groups, and there was obvious pathological damage to renal tissue. Notably, in the hypothermia model group, the general state of rats was good, Scr and BUN levels were not significantly increased 24 h post-operation, pathological damage to renal tissue and renal tubular epithelial cell apoptosis were mild, NF-κB and IL-6 protein expression was increased, and Bax protein expression was decreased. In conclusion, the induction of AKI by I/R at 20–25 °C has a high successful modeling rate and a low mortality rate. It may affect the stability of animal models by down-regulating the NF-κB/IL-6 signaling pathway and the NF-κB/Bax pathway through its anti-apoptotic and anti-inflammatory effects.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105276"},"PeriodicalIF":2.3,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supplemental effect of metallic nanoparticles on cryopreserved semen quality, lipid peroxidation, sperm head ultrastructure and field fertility levels in crossbred (Hampshire x Niang Megha) boars","authors":"Manoj Kumar Kalita ,&nbsp;Prithviraj Mazumdar Baruah ,&nbsp;Kutubuddin Ahmed ,&nbsp;Sudip Sinha ,&nbsp;Shantanu Tamuly ,&nbsp;Sourabh Deori ,&nbsp;Rahul Katiyar ,&nbsp;Sayed Nabil Abedin ,&nbsp;Prerona Patowary ,&nbsp;Raju Dewry ,&nbsp;Jitumoni Das ,&nbsp;Biplab Sarkar ,&nbsp;Gautam Khargharia ,&nbsp;Govindasamy Kadirvel","doi":"10.1016/j.cryobiol.2025.105277","DOIUrl":"10.1016/j.cryobiol.2025.105277","url":null,"abstract":"<div><div>Reactive oxygen species (ROS) produced by sperm contributes to oxidative stress (OS), leading to a decline in sperm quality. Different antioxidants have shown potential in preventing such damage. The present study explored the effect of metallic nanoparticle (NPs) supplementation on sperm quality in cryopreserved boar spermatozoa. Forty-two (42) ejaculates (7 ejaculates per animal) were collected from six (6) physically and sexually healthy and fertile Lumsniang (Hampshire x Niang Megha) crossbred boars, aged 18–42 months and then diluted in Beltsville thawing solution (BTS) extender containing the different treatments (CON: no NP addition; 10 μM ZnO NPs, 1 μg Se NPs and 0.192 mg Fe₃O₄ NPs per mL of semen). Semen was diluted, packed into 0.5 mL French medium straws, and subsequently frozen in liquid nitrogen (LN₂). Sperm quality, lipid peroxidation (LPO), antioxidant enzyme levels, ultrastructural sperm head morphology and field fertility levels were determined. Significantly (p &lt; 0.05) higher sperm <em>in vitro</em> quality attributes were recorded in the zinc oxide (ZnO) and selenium (Se) NP treatments in comparison to CON whereas iron-oxide (Fe₃O₄) NP treatment significantly (p &lt; 0.05) lowered sperm quality. Post-thaw LPO levels significantly (p &lt; 0.05) decreased in the ZnO and Se NP groups when compared to CON. LPO levels were significantly (p &lt; 0.05) higher in the Fe₃O₄ NP group. The ZnO and Se NP treatments showed significantly (p &lt; 0.05) higher post-thaw antioxidant enzyme levels. Ultrastructurally, Group 1 spermatozoa were significantly (p &lt; 0.05) higher and Group 2 were significantly (p &lt; 0.05) lower in the ZnO and Se NP supplemented group in comparison to CON. No significant (p &gt; 0.05) difference was observed in the <em>in vivo</em> pregnancy rate; however, the ZnO and Se NP treatments significantly (p &lt; 0.05) enhanced the average litter sizes at birth and weaning compared to CON. In conclusion, 10 μM ZnO and 1 μg Se NPs improved post-thaw quality of boar spermatozoa and safeguarded the ultrastructure of their membranes. On the other hand, 0.192 mg Fe₃O₄ NPs had deleterious effects on sperm <em>in vitro</em> quality.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105277"},"PeriodicalIF":2.3,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144254748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rat one-cell embryo vitrification in F344, Long-Evans, and SD strain via small-volume vitrification and rapid warming in cryotubes","authors":"Shinsuke Seki ,&nbsp;Toshiaki Kawabe ,&nbsp;Kazuaki Matsumura ,&nbsp;Misako Higashiya ,&nbsp;Takanori Oikawa ,&nbsp;Yuriko Fujii ,&nbsp;Megumi Yano ,&nbsp;Wataru Yamazaki ,&nbsp;Tomoo Eto","doi":"10.1016/j.cryobiol.2025.105260","DOIUrl":"10.1016/j.cryobiol.2025.105260","url":null,"abstract":"<div><div>Genome-edited animals can be created by introducing CRISPR/Cas9 systems into one-cell stage embryos, even in non-mice embryos. We developed a vitrification method for rat one-cell embryos of the F344 inbred, Long-Evans, and SD strains. Successful cryopreservation requires the avoidance of intracellular ice formation (IIF). We hypothesized that a new cryopreservation method could be developed by rapid warming to avoid IIF during warming, via cryopreservation in conventional cryotubes using small-volume vitrification and rapid warming. When cryopreserved one-cell embryos in a cryotube with 15 μL cryopreservation solution (mixture of 5 μL 5 % propylene glycol and 10 μL PEPeS) were warmed by adding 1 ml of 0.3 M sucrose solution at 50 °C, they developed to term at 67.2 % (F344), 56.3 % (Long-Evans), and 65.0 % (SD), which were comparable with those (60.3 %, 58.3 %, 67.0 %, respectively) of non-cryopreserved control embryos. Thus, rat one-cell embryos from several strains can be cryopreserved even with cryotubes via rapid warming.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105260"},"PeriodicalIF":2.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144243323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an automated device for the optimization of oocyte and embryo vitrification","authors":"Haoyang Lin ,&nbsp;Chenxi Liu ,&nbsp;Yukun Cao ,&nbsp;Xinli Zhou","doi":"10.1016/j.cryobiol.2025.105275","DOIUrl":"10.1016/j.cryobiol.2025.105275","url":null,"abstract":"<div><div>Oocyte/embryo vitrification is one of the basic techniques employed in assisted reproduction and fertility preservation. However, the current process typically requires manual operation by experienced embryologists, which is both time-consuming and exhibits inconsistent outcomes. To resolve this issue, we herein developed an automated vitrification device for oocytes/embryos. The device consists of a microfluidic mixing unit, a microgrid capillary, and a mechanical sliding unit. The microfluidic mixing unit was adopted to determine continuous changes in cryoprotective agent (CPA) concentration, reducing osmotic damage during CPA loading/removal; and the microgrid capillary was used to load/remove the CPA and vitrify the oocytes/embryos in the same carrier so as to reduce cellular loss due to cell transfer. The medical absorbent dressing was placed under the carrier, and the excess liquid was absorbed to minimize the remaining CPA solution after CPA loading. Eight different loading/removal curves were developed for CPA loading and removal protocols, and we conducted oocyte vitrification with automated vitrification equipment. Our results revealed that a quadratic function-based, loading-removal curve achieved the highest oocyte survival rate within an 8-min loading and removal duration. In addition, there was no significant difference in oocyte survival rates between the automated vitrification device and the Cryotop multi-step equilibration method (90.33 % and 94.33 %, respectively); nor did the two methods differ in terms of survival or hatching rates of 8-cell embryos. The current system automates and standardizes the oocyte/embryo vitrification process while achieving survival and developmental potential.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105275"},"PeriodicalIF":2.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144243321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viewing heat through ice: An infrared camera monitors hydrogel freezing and thawing during cryoapplication","authors":"Gennadiy O. Kovalov ,&nbsp;Mykola O. Chyzh ,&nbsp;Vyacheslav Yu Globa ,&nbsp;Oleksandr F. Todrin ,&nbsp;Galyna V. Shustakova ,&nbsp;Eduard Yu Gordiyenko ,&nbsp;Yuliya V. Fomenko ,&nbsp;Oleh V. Ivakhnenko ,&nbsp;Polina O. Kofman ,&nbsp;Sergey N. Shevchenko","doi":"10.1016/j.cryobiol.2025.105259","DOIUrl":"10.1016/j.cryobiol.2025.105259","url":null,"abstract":"<div><div>Cryosurgery employs a safe and relatively simple technique of exposure and is an advantageous and highly rated method. For its effective application, it is necessary to control both the volume of the expanding freezing zone and volumetric thermal field dynamics. The aim of this study was to perform a thermal imaging study of freezing and thawing in a model system (gel phantom) to predict the dynamics of the freezing zone during cryodestruction of biological tissues in vivo. Here, the thermal imager is an effective tool for demonstrating the surface temperature distribution. We have studied how the observed infrared image relates to the distribution and change of the thermal field in depth. For this purpose, we created test measuring equipment for simultaneous analysis of the dynamics of thermal fields on the surface, video recording of freezing and thawing on the surface as well as in the depth of the gel phantom, measuring the temperature at any given point in the depth and modeling in the zone of low temperature exposure of vessels with different blood flow parameters. It was revealed that with a modelled vessel in the low-temperature exposure zone, the surface thermal fields deformed and they gained the shape of butterfly wings. Our experimental study in a gel phantom is supported by numerical calculations, demonstrating how the freezing zone and thermal isotherms on the surface and in depth evolve under real conditions, thereby providing a basis for assessing the cryoeffect time and intensity in practice.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"120 ","pages":"Article 105259"},"PeriodicalIF":2.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144243322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}