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Antioxidant activity and protective effect of hydroxy derivatives of chalcones for sterlet (Acipenser ruthenus, Linnaeus, 1758) sperm 查尔酮羟基衍生物对小鲟(Acipenser ruthenus, Linnaeus, 1758)精子的抗氧化活性及保护作用
IF 2.3 3区 生物学
Cryobiology Pub Date : 2025-01-01 DOI: 10.1016/j.cryobiol.2024.105193
V.P. Osipova , M.N. Kolyada , M.A. Polovinkina , A.D. Kolumbet , E.N. Ponomareva , A.V. Velikorodov
{"title":"Antioxidant activity and protective effect of hydroxy derivatives of chalcones for sterlet (Acipenser ruthenus, Linnaeus, 1758) sperm","authors":"V.P. Osipova ,&nbsp;M.N. Kolyada ,&nbsp;M.A. Polovinkina ,&nbsp;A.D. Kolumbet ,&nbsp;E.N. Ponomareva ,&nbsp;A.V. Velikorodov","doi":"10.1016/j.cryobiol.2024.105193","DOIUrl":"10.1016/j.cryobiol.2024.105193","url":null,"abstract":"<div><div>The aim of this work is to study the effect of adding hydroxy derivatives of chalcones to the basic cryomedium on the ability of sterlet sperm to utilize superoxide and hydrogen peroxide, the intensity of lipid peroxidation of male fish germ cells, and their viability both before cryopreservation and after 3 days of freezing at liquid nitrogen temperature. The ability of phenolic derivatives of chalcones to increase the superoxide dismutase and catalase activities of sterlet sperm and to reduce the intensity of lipid peroxidation has been established. The antioxidant activity of the derivatives exceeds the effect of Trolox, which inhibits the functioning of the enzyme component of the antioxidant protection of fish sperm and promotes lipid peroxidation of fish sperm before cryopreservation. A beneficial effect of hydroxy derivatives of chalcones on the motility parameters of thawed sperm has been shown, indicating their ability to increase the cryoresistance of sperm in such a promising aquaculture species as sterlet.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"118 ","pages":"Article 105193"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The role of microRNA in the regulation of hepatic metabolism and energy-expensive processes in the hibernating dormouse microRNA在冬眠睡鼠肝脏代谢和能量消耗过程中的调节作用。
IF 2.3 3区 生物学
Cryobiology Pub Date : 2025-01-01 DOI: 10.1016/j.cryobiol.2024.105191
W. Aline Ingelson-Filpula , Anna Kübber-Heiss , Johanna Painer , Gabrielle Stalder , Hanane Hadj-Moussa , Fabrice Bertile , Caroline Habold , Sylvain Giroud , Kenneth B. Storey
{"title":"The role of microRNA in the regulation of hepatic metabolism and energy-expensive processes in the hibernating dormouse","authors":"W. Aline Ingelson-Filpula ,&nbsp;Anna Kübber-Heiss ,&nbsp;Johanna Painer ,&nbsp;Gabrielle Stalder ,&nbsp;Hanane Hadj-Moussa ,&nbsp;Fabrice Bertile ,&nbsp;Caroline Habold ,&nbsp;Sylvain Giroud ,&nbsp;Kenneth B. Storey","doi":"10.1016/j.cryobiol.2024.105191","DOIUrl":"10.1016/j.cryobiol.2024.105191","url":null,"abstract":"<div><div>The garden dormouse (<em>Eliomys quercinus</em>) is a fat-storing mammal that undergoes annual periods of hibernation to mitigate the effects of food scarcity, low ambient temperatures, and reduced photoperiod that characterize winter. Like other hibernating species, this animal suppresses its metabolic rate by downregulating nonessential genes and processes in order to prolong available energy stores and limit waste accumulation throughout the season. MicroRNAs (miRNAs) are short, single-stranded, noncoding RNAs that bind to mRNA and mediate post-transcriptional suppression, making miRNA ideal for modulating widespread changes in gene expression, including global downregulation typified by metabolic rate depression. Using next-generation sequencing, we analyzed an RNA-seq dataset to determine which miRNAs are differentially regulated during hibernation in the dormouse liver. We found that the expression of 19 miRNAs was altered during hibernation; however, only one major miRNA (miR-34a-5p) remained significantly downregulated after correcting for false discovery rate. Gene Ontology, KEGG Pathway Analysis, and DIANA-miRPath predicted that energy metabolism, nuclear-related functions such as histone binding, chromatin- and chromosomal binding, and the cell cycle are processes that may be differentially regulated during hibernation due to miRNA regulation. Taken together, our data suggest that miRNA influence appears to be strongly directed toward suppressing energy-intensive processes in the nucleus hence contributing to extend the animal's endogenous fuel reserves for the duration of hibernation.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"118 ","pages":"Article 105191"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Curcumin–loaded niosomal nanocarriers offer a promising approach to improve quality characteristics, apoptotic gene expression, and flow cytometry assessments of stallion spermatozoa after thawing 装载姜黄素的乳质体纳米载体是一种很有前途的方法,可以改善解冻后种马精子的质量特征、凋亡基因表达和流式细胞术评估。
IF 2.3 3区 生物学
Cryobiology Pub Date : 2024-12-29 DOI: 10.1016/j.cryobiol.2024.105188
Niloofar Nasiri-Foomani, Saeed Hassani, Mojtaba Najafi, Firooz Samadi
{"title":"Curcumin–loaded niosomal nanocarriers offer a promising approach to improve quality characteristics, apoptotic gene expression, and flow cytometry assessments of stallion spermatozoa after thawing","authors":"Niloofar Nasiri-Foomani,&nbsp;Saeed Hassani,&nbsp;Mojtaba Najafi,&nbsp;Firooz Samadi","doi":"10.1016/j.cryobiol.2024.105188","DOIUrl":"10.1016/j.cryobiol.2024.105188","url":null,"abstract":"<div><div>The optimization of cryopreservation media to reduce oxidative damage on post-thaw spermatozoa is crucial. This research aimed to assess the antioxidant properties of curcumin-loaded niosomal nanocarriers (CurLNN) on the functional characteristics, the relative expression of apoptotic genes, and flow cytometry assessments of apoptotic-like changes, reactive oxygen species production (ROS), mitochondrial membrane potential, and chromatin integrity in stallion spermatozoa following thawing. Twenty-five ejaculates were diluted in INRA96 freezing media supplemented with 20 μM of either curcumin (Cur) or CurLNN and then cryopreserved. Results demonstrated that spermatozoa treated with Cur, particularly CurLNN, exhibited higher percentages of total and progressive motility, as well as VAP, VSL, and STR kinematics. Additionally, the functionality of the plasma membrane was enhanced, and there was a decrease in spermatozoa abnormality (<em>P</em> &lt; 0.05). The incorporation of cryo-diluent medium with Cur and CurLNN led to increased viability (<em>P</em> &lt; 0.05), while simultaneously reducing the levels of MDA. Flow cytometry analysis revealed a significant enhancement in mitochondrial potential activity, a reduction (<em>P</em> &lt; 0.05) in ROS production, and an increase (<em>P</em> &lt; 0.05) in the proportion of live and a decrease in late apoptotic stallion post-thawed spermatozoa treated with both Cur and CurLNN. Moreover, the relative expression of the Bcl<sub>2</sub> anti-apoptotic gene increased (<em>P</em> &lt; 0.05) by the addition of cur and CurLNN in cryo-diluent extender, while inclusion of CurLNN in cryo-diluent medium resulted in a significant reduction (<em>P</em> &lt; 0.05) in the relative expression of the Bax pro-apoptotic gene in stallion post-thawed spermatozoa. In summary, the findings of this study demonstrated that CurLNN exhibits enhanced antioxidant properties, which contribute to the improved functional quality of spermatozoa by alleviating oxidative stress during the cryopreservation process.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"118 ","pages":"Article 105188"},"PeriodicalIF":2.3,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
LPA reduces the apoptosis of cryopreserved porcine skin-derived stem cells by inhibiting the regulatory factor TNF-α LPA通过抑制调节因子TNF-α减少猪皮肤源性干细胞的凋亡。
IF 2.3 3区 生物学
Cryobiology Pub Date : 2024-12-24 DOI: 10.1016/j.cryobiol.2024.105189
Xin-Xiang Xie , Jia-Dong Sun , Ming-Xin Zang , Geng Zhang , Chun-Xiao Li , Xiang-Wei Zhai , Wei Shen , Wei Ge , Shun-Feng Cheng
{"title":"LPA reduces the apoptosis of cryopreserved porcine skin-derived stem cells by inhibiting the regulatory factor TNF-α","authors":"Xin-Xiang Xie ,&nbsp;Jia-Dong Sun ,&nbsp;Ming-Xin Zang ,&nbsp;Geng Zhang ,&nbsp;Chun-Xiao Li ,&nbsp;Xiang-Wei Zhai ,&nbsp;Wei Shen ,&nbsp;Wei Ge ,&nbsp;Shun-Feng Cheng","doi":"10.1016/j.cryobiol.2024.105189","DOIUrl":"10.1016/j.cryobiol.2024.105189","url":null,"abstract":"<div><div>Preserving the viability and functionality of stem cells during cryopreservation is crucial for their successful application in regenerative medicine. The aim of this study is to investigate the effect of lysophosphatidic acid (LPA) on reducing the apoptosis of cryopreserved porcine skin-derived stem cells (pSDSCs). Our findings revealed that LPA, at a concentration of 5 μM, significantly improved viability and reduced apoptosis in cryopreserved pSDSCs. Furthermore, our data indicated that LPA enters pSDSCs through receptor type 1 (LPAR1). In cryopreserved pSDSCs, after LPA treatment, the expression level of tumor necrosis factor alpha (TNF-α) protein decreased, suggesting TNF-α involvement in the regulation of the anti-apoptotic process. Additionally, we found that resiquimod (R848), a TNF-α activator, increased the level of apoptosis in cryopreserved pSDSCs. When cryopreserved pSDSCs were treated with both LPA and R848, the protective effect of LPA against apoptosis was decreased. In conclusion, our study demonstrates that LPA could effectively counteract the effect of TNF-α-induced apoptosis, thereby enhancing the survival rates of cryopreserved pSDSCs. Importantly, this study explored a novel mechanism of reducing apoptosis in cryopreserved stem cells.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"118 ","pages":"Article 105189"},"PeriodicalIF":2.3,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ultrasound-based geometric modeling of the human ovary with applications to cryopreservation 基于超声波的人类卵巢几何建模及其在冷冻保存中的应用。
IF 2.3 3区 生物学
Cryobiology Pub Date : 2024-12-21 DOI: 10.1016/j.cryobiol.2024.105187
Rounak K. Baheti , Prem K. Solanki , Sally Ahmed , Angela Baerwald , Yoed Rabin
{"title":"Ultrasound-based geometric modeling of the human ovary with applications to cryopreservation","authors":"Rounak K. Baheti ,&nbsp;Prem K. Solanki ,&nbsp;Sally Ahmed ,&nbsp;Angela Baerwald ,&nbsp;Yoed Rabin","doi":"10.1016/j.cryobiol.2024.105187","DOIUrl":"10.1016/j.cryobiol.2024.105187","url":null,"abstract":"<div><div>Successful cryopreservation of the whole ovary outside of the body, while a woman undergoes cancer treatments, may help preserving fertility and regaining hormone balance during recovery. One of the key challenges in whole ovary cryopreservation is adequately loading the organ with cryoprotective agents (CPAs). Another notable challenge in developing the application is the lack of geometric data needed for designing matching thermal protocols. The objective of the current study is twofold: (i) to develop an effective geometric reconstruction method for the ovary, based on transvaginal ultrasound (TVUS) data, and (ii) to perform a pilot study on the thermal effects associated with CPA loading with application to vitrification. This study includes screening of 127 TVUS imaging datasets of ovaries from healthy ovulatory participants, reconstruction of 14 geometric models, and thermally analyzing two representative geometric models of low and high mature follicles-to-organ volume ratios. Results of this study demonstrate that the proposed reconstruction method is faster and more accurate than that facilitated by commercially available software (SonoAVC, GE Healthcare). Two extremes were investigated: (1) complete vitrification of the ovary, and (2) crystallization of mature follicles while the remaining ovarian stroma vitrifies. CPA loading into the mature follicles is considered an outstanding cryopreservation challenge, but with very little impact on long-term fertility preservation. Results of this study suggest that ovarian preservation by vitrification is feasible when sufficient CPA loading is achieved, while identifying the most suitable CPA for the task remains a challenge beyond the scope of the current study.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"118 ","pages":"Article 105187"},"PeriodicalIF":2.3,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An efficient combination of a CPA loading protocol, metal container, and storage method for successful articular cartilage vitrification CPA加载方案、金属容器和储存方法的有效结合,成功实现关节软骨玻璃化。
IF 2.3 3区 生物学
Cryobiology Pub Date : 2024-12-18 DOI: 10.1016/j.cryobiol.2024.105185
Mohammadhamed Shahsavari , Ana Paula Ribeiro Rodrigues , Janet A.W. Elliott , Nadr M. Jomha
{"title":"An efficient combination of a CPA loading protocol, metal container, and storage method for successful articular cartilage vitrification","authors":"Mohammadhamed Shahsavari ,&nbsp;Ana Paula Ribeiro Rodrigues ,&nbsp;Janet A.W. Elliott ,&nbsp;Nadr M. Jomha","doi":"10.1016/j.cryobiol.2024.105185","DOIUrl":"10.1016/j.cryobiol.2024.105185","url":null,"abstract":"<div><div>Successful cryopreservation of articular cartilage (AC) depends on a number of variables, including heat transfer, sample packaging for cryogenic storage, cryoprotectant agent (CPA) concentration (toxicity), and CPA permeation into AC. In the first experiment of the present study, we used a combination of our established vitrification protocol (430-min multi-step loading Protocol 8) and the metal Ovarian Tissue Cryosystem (OTC) as a closed vitrification-storage-rewarming container to vitrify 7-mm diameter porcine osteochondral dowels with 2 methods of storage (storage in the OTC with or without a surrounding vitrification solution). In the second experiment, in an attempt to introduce a more reproducible and safe vitrification protocol, we employed our successful Protocol 8 with the OTC as the CPA loading container and a cryobag as the storage container (with or without surrounding vitrification solution). In both experiments, post-warming normalized chondrocyte viabilities were assessed. The results of the first experiment demonstrated that a combination of the established step-wise CPA loading-vitrification-rewarming protocol (Protocol 8) with the metal OTC as the only container through all steps of CPA loading, vitrification, and rewarming produced a positive result. In the second experiment, combination of Protocol 8, the OTC as the CPA loading system and a cryobag as the storage container in both vitrified groups with or without solution resulted in high normalized chondrocyte viabilities (94.63 % and 96.17 %, respectively) post vitrification in 7-mm diameter porcine osteochondral dowels. Overall, this study demonstrated that by employing our established Protocol 8, using the metal OTC as a CPA loading container and a cryobag as the storage method we could achieve a reproducible method for clinical applications involving vitrified articular cartilage.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"118 ","pages":"Article 105185"},"PeriodicalIF":2.3,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142784412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Glycerol improves the viability of a cryopreserved choanoflagellate 甘油提高了冷冻保存的鞭藻的活力。
IF 2.3 3区 生物学
Cryobiology Pub Date : 2024-12-12 DOI: 10.1016/j.cryobiol.2024.105183
Sheel Chandra , Florentine U. Rutaganira
{"title":"Glycerol improves the viability of a cryopreserved choanoflagellate","authors":"Sheel Chandra ,&nbsp;Florentine U. Rutaganira","doi":"10.1016/j.cryobiol.2024.105183","DOIUrl":"10.1016/j.cryobiol.2024.105183","url":null,"abstract":"<div><div>The colonial choanoflagellate <em>Salpingoeca rosetta</em> is a tractable model system for studying the origins of multicellularity, but long-term storage strategies for this species have not been tested. In this study, we probed each stage of cryopreservation (cooling, long-term storage, recovery) to identify the optimal protocol for recovery of <em>S. rosetta</em> and co-cultured bacterial cells. Dimethyl sulfoxide (Me2SO; commonly referred to as DMSO), the current cryoprotective agent (CPA) standard, proved to be worse than glycerol at comparable concentrations. Samples treated with either CPA at 5 % showed the poorest recovery. Our results identified 15 % glycerol as the most effective CPA for both <em>S. rosetta</em> and <em>Echinicola pacifica</em>. We also determined that ultra-low temperature freezers can be sufficient for short-term storage. We propose 15 % glycerol and liquid phase nitrogen as the standard cryopreservation protocol for <em>S. rosetta</em> cultures and as a starting point for testing long-term storage strategies for other choanoflagellates and heterotrophic protists.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"118 ","pages":"Article 105183"},"PeriodicalIF":2.3,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of low temperature stress on cold-resistant substances and gene expression in Spodoptera frugiperda (Lepidoptera: Noctuidae) 低温胁迫对夜蛾类夜蛾抗冷物质及基因表达的影响
IF 2.3 3区 生物学
Cryobiology Pub Date : 2024-12-05 DOI: 10.1016/j.cryobiol.2024.105166
Huawei Ren, Junrui Zhi, Dingyin Li, Wenbo Yue, Li Liu
{"title":"Effects of low temperature stress on cold-resistant substances and gene expression in Spodoptera frugiperda (Lepidoptera: Noctuidae)","authors":"Huawei Ren,&nbsp;Junrui Zhi,&nbsp;Dingyin Li,&nbsp;Wenbo Yue,&nbsp;Li Liu","doi":"10.1016/j.cryobiol.2024.105166","DOIUrl":"10.1016/j.cryobiol.2024.105166","url":null,"abstract":"<div><div>The fall armyworm <em>Spodoptera frugiperda</em> (J. E. Smith) (Lepidoptera: Noctuidae) is a major agricultural pest. Low temperature is an important factor that limits <em>S. frugiperda</em> survival and reproduction. The physiological cold tolerance indexes of <em>S. frugiperda</em> larvae from October 2022 to February 2023 were investigated. The results showed that the cold-resistant substances in <em>S. frugiperda</em> differed significantly among different months. The water content was the lowest in January. Lipids were the highest in December and January, trehalose and protein contents were the highest in December, and glycerol content was the highest in January. The contents of lipid, protein, trehalose, and glycerol were the highest in the 6<sup>th</sup> instar larvae, whereas the water content did not differ among the larva stages. The functions of two genes, including endothelial lipase (<em>Eol1</em>) and gastric lipase (<em>Epl1</em>) that were extracted from the transcriptome analysis of <em>S. frugiperda</em> in the cold resistance were assessed. After 24 h of cold stress, the mRNA levels of <em>Eol1</em> and <em>Epl1</em> RNA interference were significantly down-regulated by 60.76 % and 82.53 %, and the survival rate of <em>S. frugiperda</em> larvae decreased. Additionally, <em>Eol1</em> and <em>Epl1</em> silencing significantly decreased the lipid content and pupal weight of <em>S. frugiperda</em>. The results indicate that <em>S. frugiperda</em> larvae can change the contents in winter to response the cold conditions and the lipids plays an important role in the cold resistance of <em>S. frugiperda.</em></div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"118 ","pages":"Article 105166"},"PeriodicalIF":2.3,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142779646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The thermodynamic principles of isochoric freezing pressure-aided supercooling 等速冷冻压力辅助过冷的热力学原理。
IF 2.3 3区 生物学
Cryobiology Pub Date : 2024-12-05 DOI: 10.1016/j.cryobiol.2024.105168
Alan L. Maida , Pedro Alejandro Perez , Cristina Bilbao-Sainz , Boris Rubinsky , Anthony N. Consiglio
{"title":"The thermodynamic principles of isochoric freezing pressure-aided supercooling","authors":"Alan L. Maida ,&nbsp;Pedro Alejandro Perez ,&nbsp;Cristina Bilbao-Sainz ,&nbsp;Boris Rubinsky ,&nbsp;Anthony N. Consiglio","doi":"10.1016/j.cryobiol.2024.105168","DOIUrl":"10.1016/j.cryobiol.2024.105168","url":null,"abstract":"<div><div>This study outlines a method for designing an isochoric (constant volume) system to reduce the supercooling preservation temperature without affecting the likelihood of ice nucleation and without the need for cryoprotective additives. The method involves a multiphase system wherein the biological material is separated from a second aqueous solution by a boundary that transfers pressure and heat but not mass. The pressure within the system is passively increased by the confined growth of ice within the secondary solution. This increased pressure in turn lowers the equilibrium freezing temperature of the biological matter, which may be utilized to lower the preservation temperature while maintaining the same degree of supercooling. For example, using this technique, the supercooling preservation temperature may be lowered from –2 °C to –5 °C without increasing the risk of ice nucleation, by ensuring the freezable phase makes up ∼17 % of the total system volume.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"118 ","pages":"Article 105168"},"PeriodicalIF":2.3,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sperm cryopreservation and reproductive characteristics in the beauty snake (Elaphe taeniura) as a model for endangered Colubridae 作为濒危蛇科典型的美蛇的精子冷冻保存及生殖特征。
IF 2.3 3区 生物学
Cryobiology Pub Date : 2024-12-04 DOI: 10.1016/j.cryobiol.2024.105184
P. Banchi , F. Solanes-Vilanova , M. Cesauri , L. Spanoghe , K. Chiers , A. Van Soom , T. Hellebuyck , G. Rizzoto
{"title":"Sperm cryopreservation and reproductive characteristics in the beauty snake (Elaphe taeniura) as a model for endangered Colubridae","authors":"P. Banchi ,&nbsp;F. Solanes-Vilanova ,&nbsp;M. Cesauri ,&nbsp;L. Spanoghe ,&nbsp;K. Chiers ,&nbsp;A. Van Soom ,&nbsp;T. Hellebuyck ,&nbsp;G. Rizzoto","doi":"10.1016/j.cryobiol.2024.105184","DOIUrl":"10.1016/j.cryobiol.2024.105184","url":null,"abstract":"<div><div>Sperm collection and cryopreservation are key technologies for assisted reproduction, with post-mortem sperm collection being the main tool to prevent the decline of endangered species. The present study describes post-mortem sperm collection and two cryopreservation protocols in beauty snakes (<em>Elaphe taeniura</em>), as a model for <em>Colubridae</em>. The vasa deferentia of 18 snakes were collected post-mortem and incubated for 30 min at room temperature to retrieve sperm by float up. Afterwards, fresh sperm was analysed, and 11 samples were diluted into either a single or a double-step TEST-egg yolk-based extender to reach a final concentration of 12 % glycerol. Anatomical and histological analyses of the reproductive organs revealed that those on the right side were larger, heavier, and positioned more cranially than the left ones (P = 0.007, P = 0.003, and P = 0.0002, respectively), but sperm parameters did not differ. Successful sperm retrieval was achieved in 61.1 % of snakes, with positive correlations between seminiferous tubules length and body weight (P = 0.0004) and between seminiferous tubules length and body length (P = 0.001). Sperm-producing animals were heavier and longer compared to azoospermic ones (P = 0.04 and P = 0.04 respectively). Specifically, males longer than 130 cm and heavier than 177g have higher reproductive potential. Both cryopreservation protocols yielded comparable results, preserving sperm viability, motility, and morphology. Therefore, a single-step protocol should be preferred due to its technical simplicity. In conclusion, we described for the first time the reproductive features and characteristics of spermatozoa collected post-mortem in beauty snakes. Although the described protocols are effective, further research is warranted to optimize these techniques.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"118 ","pages":"Article 105184"},"PeriodicalIF":2.3,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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