Effect of long-term storage on the quality of cryopreserved sperm of the Pangasius nasutus (Bleeker, 1863)

IF 2.3 3区生物学 Q2 BIOLOGY

Cryobiology Pub Date : 2025-03-11 DOI:10.1016/j.cryobiol.2025.105219

Nurizzati Idris , Donald Torsabo , Muhammad Yazed Abduh , Ambok Bolong Abol-Munafi , Noordiyana Mat Noordin , Ivan Chong Chu Koh

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Abstract

In the present study, we investigated the effects of storage for 3, 6, 9 and 12 months on cryopreserved sperm of P. nasutus, in 10 % MeOH as a cryoprotectant with 90 % 0.9 % NaCl as an extender. Sperm quality on motility, fertilization, hatching, and deformity rates were investigated using 9-months cryopreserved sperm. Determination on the effects of different sperm to egg ratios also was evaluated using 1-year cryopreserved sperm. Post-thaw motility (PTM) of cryopreserved sperm within storage period was significantly lower compared to the initial motility of fresh sperm (51.67 ± 2.4 %). However, there was no significant change of PTM during 12 months of storage (3 months, 33.3 ± 2.33 %; 6 months, 32.0 ± 2.52; 9 months, 32.67 ± 1.76; 12 months, 33.33 ± 1.67). In addition, the motility duration was unaffected by storage as there was no significant difference compared to fresh samples. Besides that, there were no significant differences in motility, fertilization and deformity rates for fresh and 9-months cryopreserved sperm except for hatching rate. Samples stored for 1 year resulted in high fertilization across all sperm to egg ratios, showing no difference compared to fresh sperm. Cryopreserved sperm exhibited a significant higher hatching rate (9.24 ± 4.68 %) at the sperm to egg ratio of 300,000:1 compared to 150,000:1 (3.44 ± 1.7 %). However, no significant difference in deformity rates for fresh and cryopreserved at sperm to egg ratio of 300,000:1. The newly developed cryopreservation protocol using methanol successfully preserved sperm quality, maintaining fertilization rates comparable to fresh sperm. This highlights its potential application in sustainable aquaculture and conservation strategies for Pangasius nasutus.

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在本研究中，我们以 10 % 的 MeOH 作为冷冻保护剂，以 90 % 的 0.9 % NaCl 作为扩展剂，研究了冷冻保存 3、6、9 和 12 个月对鼻疽鲮精子的影响。使用冷冻保存 9 个月的精子对精子活力、受精率、孵化率和畸形率的质量进行了研究。此外，还使用冷冻保存 1 年的精子评估了不同精子与卵子比例的影响。与新鲜精子的初始活力（51.67 ± 2.4 %）相比，冷冻保存精子在保存期内的解冻后活力（PTM）明显降低。然而，在 12 个月的储存期间，PTM 没有明显变化（3 个月，33.3 ± 2.33 %；6 个月，32.0 ± 2.52；9 个月，32.67 ± 1.76；12 个月，33.33 ± 1.67）。此外，运动持续时间不受贮藏影响，与新鲜样本相比没有显著差异。此外，除孵化率外，新鲜精子和冷冻保存 9 个月的精子在活力、受精率和畸形率方面均无明显差异。保存1年的样本在所有精子与卵子比率上都有较高的受精率，与新鲜精子相比没有差异。与 150,000:1 的孵化率（3.44 ± 1.7 %）相比，冷冻保存精子在精子与卵子比率为 300,000:1 时的孵化率（9.24 ± 4.68 %）明显更高。然而，在精子与卵子的比例为 300,000:1 时，新鲜和冷冻保存的畸形率没有明显差异。新开发的甲醇冷冻保存方案成功地保持了精子质量，受精率与新鲜精子相当。这突显了其在可持续水产养殖和鼻鲮保护战略中的潜在应用。

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来源期刊

Cryobiology 生物-生理学

CiteScore

5.40

自引率

7.40%

发文量

审稿时长

56 days

期刊介绍： Cryobiology: International Journal of Low Temperature Biology and Medicine publishes research articles on all aspects of low temperature biology and medicine. Research Areas include: • Cryoprotective additives and their pharmacological actions • Cryosurgery • Freeze-drying • Freezing • Frost hardiness in plants • Hibernation • Hypothermia • Medical applications of reduced temperature • Perfusion of organs • All pertinent methodologies Cryobiology is the official journal of the Society for Cryobiology.