在球体中超高速冷冻产生比在吸管中更高的抗冻性，但仍然不如传统的慢速冷冻的种马精子

IF 2.1 3区生物学 Q2 BIOLOGY

Cryobiology Pub Date : 2025-09-08 DOI:10.1016/j.cryobiol.2025.105303

Francisco Larriva-González , Jefferson E. Erráez-Guaicha , Karen E. Torres-Ordóñez , Mauricio Duma , Sebastián Larriva-Salazar , Diego A. Galarza

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引用次数: 0

摘要

本研究分别用微球和吸管超快速（UR）冷冻法和常规慢速（CS）冷冻法对种马精子的抗冻性进行了研究。将4匹公马的16支射精按冷冻方法分成3份：用液氮（LN2）直接浸泡在30 μ l的小球（UR- spheres）中进行UR冷冻；将UR冷冻于0.25 ml吸管（UR- straw）中，在LN2中直接水平浸没；和CS在LN2蒸气中冻结。超高速冷冻培养基为100 mM海藻糖+ 1%牛血清白蛋白，CS冷冻培养基为5%二甲基甲酰胺。常规慢速冷冻的精子抗冻性高于两种冷冻方法（P < 0.05）。UR-Spheres比ur - straw产生更高的精子运动、活力和顶体完整性值（P < 0.05）。两组精子头部面积和周长均比新鲜和CS冷冻样品增加（P < 0.05）。综上所述，用UR-Spheres法测定种马精液的抗冻性优于UR-Straw法。

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Ultra-rapid freezing in spheres yields a higher cryoresistance than in straws but remains inferior to conventional slow freezing of stallion sperm

This study evaluated the cryoresistance of stallion sperm frozen by ultra-rapid (UR) methods using microspheres and straws or by the conventionally-slow (CS) method. Sixteen ejaculates from four stallions were each divided into three aliquots according to the freezing method: UR freezing in 30-μL spheres (UR-Spheres) by direct immersion in liquid nitrogen (LN₂); UR freezing in 0.25-mL straws (UR-Straws) by direct horizontal submersion in LN₂; and CS freezing in LN₂ vapors. Ultra-rapid freezing medium included 100 mM trehalose +1 % BSA, and the CS freezing medium contained 5 % dimethylformamide. Conventional-slow freezing yielded higher sperm cryoresistance than both UR freezing methods (P < 0.05). UR-Spheres produced higher values of sperm kinematic, viability, and acrosomal integrity than UR-Straws (P < 0.05). Sperm head area and perimeter in both UR freezing groups increased compared to fresh and CS frozen samples (P < 0.05). In conclusion, the UR-Spheres method produced greater cryoresistance of stallion semen than UR-Straw method.

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来源期刊

Cryobiology 生物-生理学

CiteScore

5.40

自引率

7.40%

发文量

审稿时长

56 days

期刊介绍： Cryobiology: International Journal of Low Temperature Biology and Medicine publishes research articles on all aspects of low temperature biology and medicine. Research Areas include: • Cryoprotective additives and their pharmacological actions • Cryosurgery • Freeze-drying • Freezing • Frost hardiness in plants • Hibernation • Hypothermia • Medical applications of reduced temperature • Perfusion of organs • All pertinent methodologies Cryobiology is the official journal of the Society for Cryobiology.