The impact of procyanidin supplementation on Turkey semen quality, oxidative stress parameters, and fertilization during preservation at −196 °C

IF 2.1 3区 生物学 Q2 BIOLOGY
Seyed Sattar Seyed Torabi , Tohid Mohammadi , Mehdi Rezaei
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引用次数: 0

Abstract

This study investigated the effects of procyanidin (PC)-supplemented semen extenders on the quality and fertility of cryopreserved turkey sperm. Semen was pooled and diluted using a glucose-based extender enriched with PC at concentrations of 10, 30, and 50 μg before freezing. Post-thaw evaluations included motility, motion characteristics, viability, plasma membrane functionality (PMF), DNA integrity, total antioxidant capacity (TAC), glutathione peroxidase (GPx) activity, superoxide dismutase (SOD) activity, and lipid peroxidation (MDA) levels. Fertility and hatchability assessments were conducted via artificial insemination using thawed semen. Extenders with 30 and 50 μg PC significantly enhanced motility, viability, PMF, TAC, and SOD activity while reducing DNA damage, abnormal morphology, and lipid peroxidation compared to other groups. Moreover, fertility rates were notably higher with PC concentrations of 30 and 50 μg. The findings highlight that adding PC at 30 or 50 μg improves the quality of cryopreserved turkey semen, optimizing fertility and hatchability outcomes.
原花青素添加对火鸡精液质量、氧化应激参数和- 196°C保存期间受精的影响
本研究研究了原花青素(PC)对冷冻火鸡精子质量和育性的影响。在冷冻前,将精液用富含PC的葡萄糖填充剂稀释,浓度分别为10、30和50 μg。解冻后评估包括运动性、运动特性、活力、质膜功能(PMF)、DNA完整性、总抗氧化能力(TAC)、谷胱甘肽过氧化物酶(GPx)活性、超氧化物歧化酶(SOD)活性和脂质过氧化(MDA)水平。利用解冻后的精液进行人工授精,评估受精率和孵化率。与其他组相比,添加30和50 μg PC的延长剂显著提高了小鼠的运动能力、活力、PMF、TAC和SOD活性,同时减少了DNA损伤、形态异常和脂质过氧化。此外,PC浓度为30和50 μg时,生育力显著提高。结果表明,添加30或50 μg的PC可提高低温保存火鸡精液的质量,优化受精率和孵化率。
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来源期刊
Cryobiology
Cryobiology 生物-生理学
CiteScore
5.40
自引率
7.40%
发文量
71
审稿时长
56 days
期刊介绍: Cryobiology: International Journal of Low Temperature Biology and Medicine publishes research articles on all aspects of low temperature biology and medicine. Research Areas include: • Cryoprotective additives and their pharmacological actions • Cryosurgery • Freeze-drying • Freezing • Frost hardiness in plants • Hibernation • Hypothermia • Medical applications of reduced temperature • Perfusion of organs • All pertinent methodologies Cryobiology is the official journal of the Society for Cryobiology.
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