Current protein & peptide science最新文献

{"title":"Optimization, Expression, Purification, and Activity Identification of Anti-PD-1 scFv.","authors":"Wan Jiandong, Yu Peining, Liu Yulin, Qin Zhiyang, Wu Haobin, Wang Feng","doi":"10.2174/0113892037434553260114054631","DOIUrl":"https://doi.org/10.2174/0113892037434553260114054631","url":null,"abstract":"<p><strong>Introduction: </strong>Anti-PD-1 monoclonal antibodies have revolutionized cancer therapy but face challenges, including immune-related adverse events, poor tumor penetration due to their large size, and high production costs. Single-chain variable fragments offer a promising alternative, with enhanced tissue penetration and the potential for cost-effective prokaryotic expression.</p><p><strong>Methods: </strong>Based on the Opdivo sequence, an anti-PD-1 scFv (L2H5) was designed using a framework region-frequency-guided optimization approach. Two sites in the light chain and five in the heavy chain FR were mutated to high-frequency residues. The gene was codon-optimized for E. coli and cloned into the pET-28a vector. The protein was expressed in E. coli BL21(DE3), purified via Ni-NTA chromatography from inclusion bodies, and refolded by dialysis. Its bioactivity was assessed by immunoblotting, cytotoxicity assay, immunofluorescence, and a luciferase-based tumor cell killing assay.</p><p><strong>Results: </strong>The codon-optimized L2H5 scFv was successfully expressed, yielding 4 mg/L after purification. Immunoblotting confirmed its specific binding to PD-1. The CCK-8 assay demonstrated no significant cytotoxicity towards normal 293T cells. Immunofluorescence revealed specific binding to PD-1 on A549 cell surfaces. In a co-culture system with tumor cells and lymphocytes, the L2H5 scFv significantly enhanced T cell-mediated tumor cell killing in a dose- and time-dependent manner.</p><p><strong>Discussion: </strong>This study connected the VH and VL region sequences of the PD-1 monoclonal antibody drug Opdivo through a linker to form a PD-1 single-chain antibody and tried to mutate and optimize the protein sequence of the antibody framework region. The activity and binding of L2H5 PD-1 scFv to PD-1 protein were verified by in vitro experiments, and the binding ability of L2H5 PD-1 scFv to PD-1 protein was verified by immunoblotting and immunofluorescence. In terms of binding ability, the results of immunoblotting showed that the corresponding bands were detected at the size position of PD-1 protein, and the results of immunofluorescence intuitively showed the binding of L2H5 PD-1 scFv protein on cells. In terms of biological activity, the results of CCK-8 experiments showed that L2H5 PD-1 scFv had no obvious toxicity to normal cells and did not affect the growth and proliferation of normal cells.</p><p><strong>Conclusion: </strong>The L2H5 anti-PD-1 scFv protein was successfully expressed by a prokaryotic vector. The expressed protein had no obvious toxicity to normal cells and retained PD-1-binding activity.</p>","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147607859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Synergistic Anti-Psoriatic Potential of Bioactive Compounds Tyrosol and Farnesol through In-Silico and In Vitro Approaches.","authors":"Pankaj Singh Patel, Rajnish Srivastava, Naveen Singh, Sunita Panchawat","doi":"10.2174/0113892037428426251217091328","DOIUrl":"https://doi.org/10.2174/0113892037428426251217091328","url":null,"abstract":"<p><strong>Introduction: </strong>Psoriasis is a chronic inflammatory skin disease affected by genetic, immune, and environmental factors. The purpose of the study was to examine the anti-psoriatic properties of Tyrosol and Farnesol against proteins associated with psoriasis, as well as their antioxidant properties.</p><p><strong>Introduction: </strong>Psoriasis is a chronic inflammatory skin disease affected by genetic, immune, and environmental factors. The purpose of the study was to examine the anti-psoriatic properties of Tyrosol and Farnesol against proteins associated with psoriasis, as well as their antioxidant properties.</p><p><strong>Materials and methods: </strong>A molecular docking simulation (AutoDock) was performed to determine the potential binding between Tyrosol and Farnesol to important targets associated with psoriasis (IL-36, IRAK4, ROR, PDE4D, cPLA2, and RIPK1). DPPH, nitric oxide, and hydrogen peroxide scavenging assays were conducted to determine the antioxidant actions.</p><p><strong>Results: </strong>Farnesol and Tyrosol competed strongly for binding to RORγt and IRAK4, respectively, and to cPLA2. Both compounds showed significant antioxidant activity, and their combination exhibited enhanced free radical scavenging activity.</p><p><strong>Discussion: </strong>Interactions and molecular behavior of the compounds, as well as in vitro results, indicate the relevance of the compounds in regulating key inflammatory and oxidative pathways in psoriasis.</p><p><strong>Conclusion: </strong>Tyrosol and Farnesol, especially in combination, are potentially therapeutic agents for the management of psoriasis due to their anti-inflammatory and antioxidant effects.</p>","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147493677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Synergistic Inhibition and Eradication of Pathogenic Bacterial Biofilms by Bacillus subtilis-derived Anti-biofilm Enzymes.","authors":"Ayisha Aman Ullah, Mawa Naeem, Fiza Khan, Khadeeja Qadeer, Raheela Rahmat Zohra, Mahnaz Ahmad","doi":"10.2174/0113892037427870260206112949","DOIUrl":"https://doi.org/10.2174/0113892037427870260206112949","url":null,"abstract":"<p><strong>Introduction: </strong>Biofilm formation is a crucial virulent attribute of pathogens that promotes their resistance to antibiotics and contributes to chronic illnesses in humans. Traditional antibiotic therapies have proven ineffective in eliminating sessile microbial populations within biofilms, necessitating the development of novel therapeutic strategies. In this study, the in vitro efficacy of the anti-biofilm enzymes derived from Bacillus subtilis was evaluated against biofilm-forming human clinical pathogens.</p><p><strong>Methods: </strong>An in vitro impact of combined anti-biofilm enzymes obtained from Bacillus subtilis C5W on the inhibition and eradication of biofilms of A. baumannii, E. aerogenes, and E.coli was monitored using a spectrophotometric microtiter plate assay.</p><p><strong>Results: </strong>Among seven clinical pathogens, three pathogens were found to be strong biofilm producers as they formed biofilm at an incubation time of 48 hours. The anti-biofilm enzymes significantly inhibited the biofilm formation of A. baumannii and E. aerogenes at an incubation time of 48 hours, with inhibition rates of 62.51% and 57.91%, respectively. In contrast, the maximum inhibition of biofilm formation in E. coli was observed at 24 hours, with an inhibition rate of 76.69%. The biofilm eradication rates were recorded to be 30.17% (A. baumannii), 46.29% (E. aerogenes), and 53.02% (E. coli) after a 24-hour incubation time. The SEM images confirmed the disruption of adhered biofilm on the glass surface and aggregation of microcolonies.</p><p><strong>Discussion: </strong>The study highlighted that Bacillus subtilis-derived enzyme combinations showed a synergistic inhibitory effect against biofilms formed by human clinical pathogens under in vitro conditions. The combined enzymatic treatment not only disrupted established biofilms but also suppressed their formation, indicating an enhanced anti-biofilm potential.</p><p><strong>Conclusion: </strong>These findings demonstrated the multi-enzyme approach as a promising and effective alternative to conventional antimicrobial approaches for managing biofilm-associated infections.</p>","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147493743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipidation & Stapled Peptides: Enhancing Stability and Pharmacokinetics.","authors":"R P Rajesh, Masilamani Selvam, Srinivasan Palaniselvam, Saravanan Ramachandran","doi":"10.2174/0113892037428456260128183846","DOIUrl":"https://doi.org/10.2174/0113892037428456260128183846","url":null,"abstract":"<p><p>The potential of peptide therapeutics is high because the targeting is highly specific, toxicity is low, and also the ability to affect complex biological determinants. These things are, however, hampered by issues like low stability, faster corrosion, low bioavailability, and low cell permeability, which limit their effectiveness in clinical conditions. This is why lipidation and peptide stapling approaches to balancing, which are highlighted in this review, are collaborators in challenging these questions. Lipidation is a covalent bonding of fatty acids or hydrophobic components, which increases the pharmacokinetics through better binding to albumin and enhancing half-life and through greater membrane interaction. Stapling also fixes α-helical structures with hydrocarbon or alternative linkers to improve resistance to proteolysis, structural stability, and intracellular transport. The sum of these changes results in a dual functionality peptide with an abnormally increased stability, bioavailability, and therapeutic effect. Other major case studies are antiviral agents targeted at SARS-CoV-2, antimicrobial peptides that include SLP-51, and stapled degraders targeting protein-protein interactions that are oncogenic. The balanced design frameworks, strategic placement of changes, linker techniques, and useful computational tools such as StaPep and Length LogD are also discussed in the review. Although issues of immunogenicity, scalability in manufacturing, and non-invasive delivery still remain, advances in peptide engineering and AI-inspired designing are also assisting in the future of dual-modified therapeutics. The combination of lipidation and stapling is a revolutionary technology that can revolutionize the development of peptide drugs in infectious diseases, cancer, and metabolic diseases.</p>","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147493757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuropeptide Y Receptor Modulators in Gut Physiology and Therapy.","authors":"Kalyani R Thombre, Nikita D Rahangdale, Krishna Radheshyam Gupta, Milind Janrao Umekar","doi":"10.2174/0113892037432130260204153437","DOIUrl":"https://doi.org/10.2174/0113892037432130260204153437","url":null,"abstract":"<p><strong>Introduction: </strong>Gut-brain communication depends on neuropeptides and hormones, and the Neuropeptide Y (NPY) family, which includes NPY, Peptide YY (PYY), and Pancreatic Polypeptide (PP), plays an important role. These peptides also affect gastrointestinal (GI) motility, secretion, nutrient uptake, and intestinal development. Diseases, including irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), gastroparesis, and obesity, are attributed to disruptions in this signaling axis. In this review, the physiological and pathological functions of NPY and its receptor subtypes (Y1, Y2, Y4, Y5) in the GI tract, the therapeutic potential of Pharmacological and natural modulators of this pathway are evaluated.</p><p><strong>Methods: </strong>An overview of recent experimental, clinical, and pharmacological evidence was carried out to describe receptor-specific activity, delineate the underlying pathophysiology, and investigate the clinical implications of NPY modulation in gastrointestinal health and disease.</p><p><strong>Results: </strong>Literature reveals that NPY signaling controls GI motility, secretion, and appetite via diverse receptor pathways. Low NPY levels are often linked with diarrheal manifestations of IBD, while PYY-mediated ileal brake supports enhanced nutrient absorption. New therapeutic strategies are promising: Y1 receptor agonists can reduce diarrhoea, Y1 antagonists can be useful in constipation, and Y2/Y5 modulators may be useful in some conditions, including obesity and gastroparesis.</p><p><strong>Discussion: </strong>The existing evidence confirms the role NPY receptors play in the GI homeostasis and disease pathology. The modeling of precision-based pharmacological interventions facilitates an enhanced comprehension of receptor-based interactions. Yet, the difference in the study design and the paucity of long-term clinical data are also significant weaknesses.</p><p><strong>Conclusion: </strong>The NPY system is an essential part of the gut-brain axis. Inhibition of Y-receptor pathways is an attractive approach to promote GI functionality and bridge therapeutic gaps in functional and inflammatory gastrointestinal diseases.</p>","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Production, Properties, and Potential Food Applications of Proteins and Peptides from Marine Sources and their Structure-Function Relationship.","authors":"Esmaeel Mirzakhani, Chi Ching Lee, Tolulope Joshua Ashaolu, Kasim Sakran Abass, Fereshteh Ansari, Hadi Pourjafar","doi":"10.2174/0113892037441673260131191900","DOIUrl":"https://doi.org/10.2174/0113892037441673260131191900","url":null,"abstract":"<p><p>The oceans, covering over 70% of the Earth's surface, represent a vast and underexplored source of proteins and bioactive peptides with unique structures and functionalities. This review comprehensively examines the production, properties, and food applications of these marine-derived compounds, with a specific focus on their structure-function relationships. We highlight that peptides derived from fish, algae, and shellfish by-products exhibit a wide range of bioactivities, including potent antioxidant, antimicrobial, antihypertensive, and anti-inflammatory effects. These healthpromoting properties are intrinsically linked to their amino acid sequences and structural features, such as molecular weight and specific configurations. While enzymatic hydrolysis is the primary method for their production, challenges in cost-effective large-scale purification persist. The review further details their successful application in functional foods to improve nutritional value, enhance shelf-life, and promote health. Finally, we conclude that to fully harness this potential, future efforts must prioritize sustainable sourcing, advanced extraction technologies, and robust clinical trials to bridge the gap between laboratory research and commercial application.</p>","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Peptides: Structure, Mechanisms, and Therapeutic Potential in Combating Infectious Diseases.","authors":"Shivendra Verma, Ranu Singh, Manoj Kumawat","doi":"10.2174/0113892037412027251205075307","DOIUrl":"https://doi.org/10.2174/0113892037412027251205075307","url":null,"abstract":"<p><p>Antimicrobial peptides (AMPs) are small, naturally occurring molecules that play a key role in the immune defense of multicellular organisms. They show strong and broad activity against many harmful agents, including bacteria, viruses, fungi, and even cancer cells. Because of their unique ability to target pathogens in multiple ways, AMPs are being studied as promising alternatives to traditional antibiotics-especially important given the growing problem of antibiotic resistance. AMPs can be grouped based on their structure, such as α-helical, β-sheet, loop, or extended forms, and by their source or biological activity. Their main ways of fighting microbes include damaging microbial membranes, disrupting essential processes inside cells, and helping regulate the host's immune system. These mechanisms make it difficult for pathogens to develop resistance, increasing their value in modern medicine. Therapeutically, AMPs are under investigation for a range of applications, including treatment of skin infections, respiratory diseases, sepsis, and some viral and cancer-related conditions. Several AMP-based drugs are in clinical trials, and a few have already reached the market. However, challenges remain, such as poor stability in the body, vulnerability to breakdown by enzymes, potential toxicity, and high production costs. Current research is focusing on improving their effectiveness through chemical modifications, smart design, and advanced delivery systems like nanoparticles. AMPs hold great potential in the search for nextgeneration anti-infective therapies.</p>","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147493701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TEAD4 Identified as a Potential Biomarker for Gastric Cancer Via Genes Associated with Liquid-Liquid Phase Separation.","authors":"Jianzhong Liu, Jingwen Zhang, Aixia Gong","doi":"10.2174/0113892037408980251201111913","DOIUrl":"https://doi.org/10.2174/0113892037408980251201111913","url":null,"abstract":"<p><strong>Introduction: </strong>Liquid-liquid phase separation (LLPS) has emerged as a critical mechanism in tumor progression, and its relevance to gastric cancer (GC) is increasingly recognized. However, the mechanisms linking LLPS to GC remain unclear. Preliminary studies suggest that the TEA domain transcription factor 4 (TEAD4) may contribute to tumorigenesis, yet its specific role in GC has not been fully elucidated. This study aims to investigate the potential influence of TEAD4 on GC and to uncover the underlying mechanisms.</p><p><strong>Methods: </strong>TEAD4 was identified as a key gene in GC by intersecting LLPS-related genes with differentially expressed genes from the TCGA-STAD dataset. TEAD4 mRNA and protein expression in GC were analyzed using TCGA, GEO, and HPA databases. Associations with clinical features, diagnostic and prognostic value, immune checkpoints, methylation, microsatellite instability (MSI), tumor mutation burden (TMB), and drug sensitivity were explored. TEAD4 expression was validated in vitro in GC cell lines via RT-qPCR and Western blot. Functional assays were performed by silencing TEAD4 in MKN45 cells to assess its effects on GC cell proliferation, migration, and invasion.</p><p><strong>Results: </strong>TEAD4, identified through LLPS-related genes, was found to be abnormally overexpressed in GC at both mRNA and protein levels (P < 0.05). High TEAD4 expression showed strong diagnostic accuracy (AUC > 0.9), correlated with tumor stage and location, and was associated with poorer overall survival (P = 5.02e-05). In GC, low TEAD4 expression was linked to increased immune cell infiltration, higher immune phenotype scores, and elevated expression of immune checkpoints. TEAD4 expression also correlated with methylation status, TMB, MSI, mutational allelic tumor heterogeneity (MATH), and chemosensitivity. In vitro, TEAD4 knockdown reduced proliferation, migration, and invasion of GC cells.</p><p><strong>Discussion: </strong>TEAD4 is associated with epigenetic modifications, immune regulation, tumor heterogeneity, and therapeutic response in GC, highlighting its potential mechanistic role in disease progression.</p><p><strong>Conclusion: </strong>TEAD4 contributes to GC progression and may serve as a biomarker for diagnosis, prognosis, and immune infiltration, providing insights for potential therapeutic strategies.</p>","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147321266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protocatechuic Acid Alleviates Neurodemyelination by Modulating PKCα-p38/MAPK Pathways in an LPC-Induced Model of Neurodegeneration.","authors":"Sonia Siddiqui, Priya Tufail, Faisal Khan, Muhammad Farooq Khalid, Farhia Khalid","doi":"10.2174/0113892037385148251207081917","DOIUrl":"https://doi.org/10.2174/0113892037385148251207081917","url":null,"abstract":"<p><strong>Introduction: </strong>Neuroinflammation, axonal damage, and alterations in extracellular matrix (ECM) protein expression are hallmarks of neurodegenerative diseases. Therapies that enhance recovery from brain injury are of significant clinical value. Therefore, this study investigated the antiinflammatory properties of protocatechuic acid (PCA).</p><p><strong>Methods: </strong>Neuroglial cocultures were prepared from P0-P1 rats. Demyelination was induced using LPC (0.003%). The effects of PCA (10 and 25 μg) on neurite outgrowth were assessed using morphometry software. Expression of COX-2, NF-κβ, PKC-α, and p38/MAPK was examined through immunostaining, SDS-PAGE, and Western blotting. Expression intensities were quantified using ImageJ software. Sustained repetitive neuronal firing was evaluated using the patch-clamp technique.</p><p><strong>Results: </strong>PCA increased neurite outgrowth in LPC-treated cultures after 72 hours in vitro. LPCinduced upregulation of ECM proteins TN-C, LN, and CSPGs was significantly reduced by PCA treatment compared with LPC controls. Similarly, PCA decreased the expression intensities of the pro-inflammatory markers NF-κβ and COX-2 relative to LPC controls. Furthermore, PCA reversed the sustained neuronal firing pattern observed in untreated LPC-exposed neurons.</p><p><strong>Discussion: </strong>Purified bioactive compounds commonly present in everyday foods show therapeutic potential for Parkinson's and Alzheimer's diseases due to their lower toxicity compared with conventional drugs. Artificial intelligence tools, such as AlphaFold and RoseTTAFold, further support drug development by predicting PCA binding modes with PKCα and P38/MAPK, thereby contributing to the design of personalized therapeutics and advancing neuroscience research.</p><p><strong>Conclusion: </strong>PCA alleviated neuroinflammation by reducing phosphorylation of PKCα and p38/MAPK.</p>","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146149531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics Analysis Reveals the Role of lncRNAs and Peptides in the Age-related Hearing Loss.","authors":"Cong Li, Danyang Liu, Kunyan He, Song Shi, Xinwei Chen","doi":"10.2174/0113892037423330251019171447","DOIUrl":"https://doi.org/10.2174/0113892037423330251019171447","url":null,"abstract":"<p><strong>Introduction: </strong>The pathogenesis of age-related hearing loss (ARHL), especially the role of long non-coding RNAs (lncRNAs) and their encoded peptides, remains incompletely understood. This study aimed to characterize expression changes in lncRNAs and peptides in the cochleae of ARHL mice and explore the potential functions of lncRNA-encoded peptides via multi- -omics analysis.</p><p><strong>Methods: </strong>C57BL/6J mice were used to establish the ARHL model. The molecular expression profiles of cochlear tissues from normal and ARHL mice were synthesized by lncRNA sequencing, peptidomics, and bioinformatics.</p><p><strong>Results: </strong>Compared with the control group, a total of 789 differentially expressed lncRNAs and 466 differentially expressed peptides were identified in the ARHL group. Functional enrichment analysis revealed their association with key pathways, including ion transport, calcium signaling, the TCA cycle, and cytoskeleton regulation, indicating broad molecular dysregulation in the aging cochlea. Notably, 64 differentially expressed lncRNAs showed high translational potential, yielding 107 novel lncRNA-encoded peptides. These were mainly short peptides, some with stabilizing hydrophobic properties suited for membrane interactions, and enriched in domains like Pkinase and C2, suggesting involvement in signal transduction.</p><p><strong>Discussion: </strong>These results emphasized that lncRNA-encoded peptides were novel regulators of ARHL, potentially regulating calcium homeostasis and mitochondrial function. The overlap of pathways such as the cytoskeleton and fatty acid metabolism indicated that the lncRNA-peptide axis drove auditory decline, providing institutional insights into the epigenetic basis of ARHL.</p><p><strong>Conclusion: </strong>Our findings suggest that lncRNA-encoded peptides are a novel class of regulatory molecules involved in the complex pathogenesis of ARHL, highlighting them as promising targets for future therapeutic strategies.</p>","PeriodicalId":10859,"journal":{"name":"Current protein & peptide science","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146050804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}