Current pharmaceutical design最新文献

{"title":"Emerging Trends in the Pharmacological and Therapeutic Potential of Ginger: From Traditional Medicine to Nanotechnological Innovations.","authors":"Jitendra Gupta, Devesh Kumar, Reena Gupta, Sumant Kumar, Mohit Kumar","doi":"10.2174/0113816128372023250324050304","DOIUrl":"https://doi.org/10.2174/0113816128372023250324050304","url":null,"abstract":"<p><strong>Introduction: </strong>Ginger (Zingiber officinale) has a long history as a culinary and medicinal plant, widely recognized in traditional medicine for the treatment of various diseases. In recent years, advances in nanotechnology have provided innovative delivery systems, enhancing ginger's bioavailability and efficacy in modern therapeutic applications. This study aims to explore ginger's pharmacological and therapeutic potential, tracing its evolution from traditional medicine to its integration into modern nanotechnological innovations. By analysing emerging trends, this study seeks to highlight ginger's diverse bioactivities and its potential to enhance therapeutic efficacy through advanced delivery systems.</p><p><strong>Methods: </strong>Literature was searched from various databases, mainly from 1984 to 2024, such as Scopus, Web of Science, Google Scholar, PubMed and Science Direct using keywords including \"Ginger\", \"Zingiber officinale\", \"Gingerols\", \"Shogaols\", \"Paradols\" and \"Nanocarriers\" and their combination. This study examines the therapeutic potential of ginger by reviewing its traditional applications and exploring nanotechnological innovations in ginger-based drug delivery systems. Nanoemulsions, liposomes, and nanoparticles were assessed for their ability to improve the stability, bioavailability, and targeted delivery of ginger's bioactive compounds.</p><p><strong>Results: </strong>Ginger's bioactive constituents, including gingerols, shogaols, and paradols, exhibited significant pharmacological activities, including anti-inflammatory, antioxidant, anticancer, antidiabetic, and gastroprotective effects. Nano-based delivery systems have shown improved stability, controlled release, and targeted delivery, thereby maximising therapeutic efficacy in treating various diseases.</p><p><strong>Conclusion: </strong>Ginger holds significant therapeutic promise in both traditional and modern medicine, mainly when used with nanotechnology for improved bioavailability and efficacy. These findings support the development of ginger-based treatments as complementary therapies in holistic healthcare. Further research and clinical trials are essential to validate these applications and optimize dosages for clinical use.</p>","PeriodicalId":10845,"journal":{"name":"Current pharmaceutical design","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and Structural Characterization of Ferrocenyl Carboxylate Containing Benzaldoxime Moieties and Preliminary Cytotoxicity against Cancer Cell Lines.","authors":"Zhipeng Ruan, Jianping Yong, Le Li, Canzhong Lu, Olagoke Zacchaeus Olatunde","doi":"10.2174/0113816128391868250430081054","DOIUrl":"https://doi.org/10.2174/0113816128391868250430081054","url":null,"abstract":"<p><strong>Aim: </strong>This study aimed to design and synthesize new ferrocene derivatives for the development of potent anticancer drugs.</p><p><strong>Background: </strong>Cancer is a major cause of death globally. Some small-molecule anticancer drugs have been used in clinics for the treatment of cancer, and several candidates are in different phases of clinical trials. However, cancer chemotherapy is still highly inadequate due to the side effects of the clinical drugs. Thus, developing novel anticancer drugs is essential.</p><p><strong>Methods: </strong>Firstly, we synthesized the R-substituted benzaldoxime intermediates (2a-2s) using R-substituted benzaldehyde (1a-1s) and hydroxylamine hydrochloride. Then, the target compounds (3a-3s) were synthesized using ferrocene carboxylic acid and R-substituted benzaldoxime intermediates (2a-2s) using DCC and DMAP as catalysts. The purity of the target compounds was determined by HPLC, and their structures were characterized using NMR, SC-XRD, and HR-ESIMS. Subsequently, the preliminary in vitro cytotoxicity against HeLa, A549, and A2780 cell lines was evaluated using MTT assay.</p><p><strong>Results: </strong>The results showed compound 3a to exhibit cytotoxicity against both HeLa and A549 cancer cell lines with IC50 values of 0.691 and 0.876 mM, respectively. Compound 3k showed potent cytotoxicity against HeLa cell lines with an IC50 value of 0.097 mM, compounds 3n and 3o exhibited potent cytotoxicity against three cancer cell lines, compound 3q showed potent cytotoxicity against HeLa cell lines with an IC50 value of 0.175 mM, while compound 3s exhibited potent cytotoxicity against HeLa and A549 cell lines with IC50 values of 0.470 and 0.298 mM, respectively.</p><p><strong>Conclusion: </strong>In this work, 19 new ferrocene derivatives containing R-substituted benzaldoxime moieties (3a- 3s) were synthesized and their structures were confirmed. Their cytotoxicity against HeLa, A549, and A2780 cell lines was tested, and the results showed that several compounds exhibited potent cytotoxicity against the tested cancer cell lines. This work developed a variety of ferrocene compounds, providing lead compounds based on ferrocene pharmacophore for the development of anticancer drugs.</p>","PeriodicalId":10845,"journal":{"name":"Current pharmaceutical design","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143994020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Satranidazole HCl-Loaded Oral Nanoparticulate Formulation for Colon Targeting and Colon Cancer Therapy Associated with Inflammatory Bowel Disease.","authors":"Debgopal Ganguly, Ananta Choudhury, Subhabrota Majumdar","doi":"10.2174/0113816128368738250414070421","DOIUrl":"https://doi.org/10.2174/0113816128368738250414070421","url":null,"abstract":"<p><strong>Background: </strong>Colon-targeted drug delivery is a crucial area of research aimed at treating local disorders like IBD, including ulcerative colitis and Crohn's disease. By delivering drugs directly to the colon, this approach enhances therapeutic efficacy and minimizes systemic toxicity. Nanoparticles are an effective vehicle for controlled drug delivery, improving treatment outcomes for colon-specific diseases.</p><p><strong>Objective: </strong>The study aimed to develop an oral nanoparticulate formulation of Satranidazole (STZ) using a solvent evaporation technique for colonic targeting and characterize its physicochemical properties, compatibility, and in vitro drug release profile.</p><p><strong>Methods: </strong>Using a modified solvent evaporation method, STZ-loaded nanoparticles (STZ-NPs) were formulated using Eudragit RS100 and RL100 polymers. Preformulation studies, including FT-IR and DSC, were performed to confirm the compatibility between the drug and polymers. The nanoparticles were evaluated in terms of entrapment efficiency, particle size, zeta potential, polydispersity index, and in-vitro drug release study.</p><p><strong>Results: </strong>The optimized formulation (F3) demonstrated the highest entrapment efficiency (83.55%) with particle sizes ranging from 107.9 nm to 302 nm and a zeta potential between -34.25 mV and +48.8 mV. In vitro drug release studies showed controlled release over 16 hours, with the optimized batch achieving 95.85% drug release, indicating effective accumulation in the inflamed colon.</p><p><strong>Conclusion: </strong>The Satranidazole-loaded nanoparticles, containing time- and pH-dependent polymers, successfully inhibited premature drug release in acidic environments and provided controlled release at colonic pH. Thus, this delivery system shows promise as an effective treatment for IBD, offering targeted drug release and reduced systemic toxicity.</p>","PeriodicalId":10845,"journal":{"name":"Current pharmaceutical design","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Probiotic Supplementation on the Sleep Quality of Humans: A Review of Results of Randomized, Blinded, Controlled Studies.","authors":"Bhagavathi Sundaram Sivamaruthi, Chaiyavat Chaiyasut, Natarajan Sisubalan, Periyanaina Kesika","doi":"10.2174/0113816128370349250413163229","DOIUrl":"https://doi.org/10.2174/0113816128370349250413163229","url":null,"abstract":"<p><p>Sleep is the key factor influencing physical and psychological health. Several factors influence sleep, including lifespan, circadian entrainment, diet, stress, and occupation. Pharmacological (for example, histamine type 1 receptor blockers) and non-pharmacological (for example, cognitive-behavioral therapy) therapeutic approaches are used to alleviate sleep disorders. The gut microbiota has an important role in the pathogenesis of sleep-related disorders. Studies suggested that restoring the healthy gut microbiota could improve sleep quality. Hence, related randomized, blinded, controlled studies were reviewed to know the impact of probiotic supplementation on sleep quality. Altered Firmicutes/Bacteroidetes ratio and reduced α-diversity were associated with insomnia, sleep deprivation and rapid eye movement sleep behavior disorder. The literature survey revealed that probiotic supplementation improved healthy subjects' sleep quality and mood states. Probiotic supplementation could improve sleep quality by improving gut microbiota, intestinal integrity, blood- -brain barrier function, brain functions and neurotransmitter regulation. The underlying mechanisms through which probiotic supplementation exerts its beneficial effects on sleep disorders remain unclear. Further research involving a variety of probiotic strains, along with long-term follow-up studies, is needed to validate the potential of probiotics as a complementary therapeutic approach for managing sleep disorders and enhancing sleep quality.</p>","PeriodicalId":10845,"journal":{"name":"Current pharmaceutical design","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutraceutical Synergy: Unraveling the Protective Effects of Methyl Gallate and Chia Seed Oil in Doxorubicin-induced Hepatic Injury and Bax/Bcl2 Imbalance.","authors":"Shakta Mani Satyam, Mohamed El-Tanani, Akheruz Zaman Ahmed, Manfredi Rizzo, Dimitrios Patoulias, Prakashchandra Shetty K, Kalyan Chakravarthi Kosuri, Rashmi Kumari, Sainath P","doi":"10.2174/0113816128366802250413160625","DOIUrl":"https://doi.org/10.2174/0113816128366802250413160625","url":null,"abstract":"<p><strong>Background: </strong>Nutraceuticals like methyl gallate and chia seed oil are gaining global attention for their therapeutic potential. This study investigates their effects on hepatocyte apoptosis and liver architecture in a doxorubicin-induced hepatotoxicity model, utilizing techniques such as TUNEL assay, immunohistochemistry (Bax & Bcl2), H&E staining, and scanning electron microscopy.</p><p><strong>Methodology: </strong>Thirty female Wistar rats were divided into five groups (n=6): Group I (Normal healthy control), Group II (Doxorubicin-intoxicated control), Group III (Doxorubicin-intoxicated + methyl gallate), Group IV (Doxorubicin-intoxicated + chia seed oil), and Group V (Doxorubicin-intoxicated + both). Liver function tests, histology, and cell apoptosis analysis were performed to assess the effects.</p><p><strong>Results: </strong>Doxorubicin-intoxicated rats (Group II) exhibited significantly elevated ALT, AST, and ALP levels (p < 0.001) and severe hepatic damage compared to controls. Group III and Group IV showed significant reductions in liver enzyme levels (p < 0.05 and p < 0.01, respectively), while Group V demonstrated the most significant decrease (p < 0.001). Immunohistochemistry revealed increased Bax and decreased Bcl2 expression in Group II (p < 0.001), which improved significantly with methyl gallate, chia seed oil, and their combination (p < 0.05 to p < 0.001). TUNEL assay showed reduced apoptotic index in treatment groups, with Group V showing the most significant reduction (p < 0.001). Scanning electron microscope (SEM) analysis confirmed restoration of hepatocyte architecture, especially in Group V.</p><p><strong>Conclusion: </strong>Methyl gallate and chia seed oil, individually and in combination, demonstrated significant hepatoprotective effects against doxorubicin-induced hepatotoxicity, with the combination showing the greatest efficacy. These nutraceuticals hold promise as adjunct therapies to reduce doxorubicin-induced liver injury.</p>","PeriodicalId":10845,"journal":{"name":"Current pharmaceutical design","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecularly Imprinted Polymers (MIPs): A Multifaceted Tool in Modern Analysis.","authors":"Priyanka Paul, Raj Kamal, Rohit Bhatia","doi":"10.2174/0113816128384530250423181805","DOIUrl":"https://doi.org/10.2174/0113816128384530250423181805","url":null,"abstract":"<p><p>Molecularly Imprinted Polymers (MIPs) are specialized synthetic materials with custom-designed molecular recognition properties. They are created by polymerizing monomers around a template molecule. MIPs play a crucial role in various analytical fields, including drug detection in biological samples, protein and peptide identification, pollutant monitoring in water and soil, and detecting additives, contaminants, and pesticide residues in food. Additionally, they aid in identifying toxins, viruses, and small molecules. Their versatility, cost-effectiveness, and reusability enhance precision and efficiency in analytical processes. As technology and knowledge in the field continue to advance, the potential applications of MIPs are expected to expand, making them a crucial asset in various scientific and industrial endeavors. In this review, various techniques, which include analyte, sample type, key analytical parameters (limit of detection, limit of quantification, relative standard deviation, recovery, and correlation coefficient), and corresponding references, along with an overview of MIP applications across multiple domains, are described.</p>","PeriodicalId":10845,"journal":{"name":"Current pharmaceutical design","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Quantitative Analysis of Solid Dosage Forms of Itopride using Raman Spectroscopy.","authors":"Iqra Naheed, Maida Ehsan, Haq Nawaz, Muhammad Irfan Majeed, Abdulrahman Alshammari, Norah A Albekairi, Arslan Ali, Tehseen Ijaz, Ayesha Saif, Haseeb Akbar, Aleena Aziz, Rida Fatima","doi":"10.2174/0113816128355113250414043255","DOIUrl":"https://doi.org/10.2174/0113816128355113250414043255","url":null,"abstract":"<p><strong>Objective: </strong>This study explores the application of Raman spectroscopy for identifying and quantifying itopride in solid dosage forms with varying concentrations of active ingredients and excipients. Raman spectroscopy provides a non-invasive, rapid, and accurate detection method that is ideal for pharmaceutical analysis.</p><p><strong>Method: </strong>The Raman spectral features of itopride in solid dosage forms were analyzed using Principal Component Analysis (PCA) and Partial Least Squares Regression Analysis (PLS-RA) as multivariate data analysis techniques.</p><p><strong>Results: </strong>PCA effectively distinguished Raman spectral data of various itopride drug samples. PLS-RA facilitated quantitative analysis, yielding an R2 value of 0.999%, indicating an excellent explanation of model variability. The root mean square error of calibration and prediction were 0.23 mg and 3.02 mg, respectively. Furthermore, PLS-RA accurately determined the active pharmaceutical ingredient concentration in unknown formulations, with a calculated concentration of 79.66/80 mg (w/w) compared to the actual concentration of 80/140 mg (w/w).</p><p><strong>Conclusion: </strong>These findings demonstrated that the concentration of itopride in pharmaceutical samples using an established Partial Least Squares Regression calibration model can be determined with reliability.</p>","PeriodicalId":10845,"journal":{"name":"Current pharmaceutical design","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143982150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration Approaches of UPLC-Q-Exactive Orbitrap-MS/MS, Network Pharmacology Molecular Docking, and Molecular Dynamics to Explore the Effective Constituents and Potential Mechanisms of S. vaniniiporus vaninii against Tumor.","authors":"Wen-Long Li, Pei-Lu Wang, Yan Xu, Mengyi Shan, Gang Cheng, Yun-Jie Sheng, Kao-Hua Liu, Bing-Qian He, Qi Shi, Hua-Qiang Li, Xiong-Yu Meng, Lu-Ping Qin","doi":"10.2174/0113816128365808250413155927","DOIUrl":"https://doi.org/10.2174/0113816128365808250413155927","url":null,"abstract":"<p><strong>Background: </strong>S. vaninii, a well-established traditional Chinese medicine with potent pharmacological effects against cancer, lacks clarity regarding its mechanism of action.</p><p><strong>Objective: </strong>To elucidate the bioactive components in S. vaninii and to elucidate their potential anticancer mechanisms.</p><p><strong>Methods: </strong>Firstly, the chemical composition of S. vaninii was characterized using UPLC-Q-Exactive Orbitrap- MS/MS technique. Subsequently, bioinformatics-related techniques were employed to elucidate the bioactive components and potential mechanisms of S. vaninii anti-tumor based on the identified chemical constituents. Finally, molecular dynamics simulation was conducted to validate the obtained results.</p><p><strong>Results: </strong>Our findings revealed the characterization of 226 constituents from S. vaninii including 30 flavonoids, 27 carbohydrates and glycosides, 26 amino acids, peptides and their derivatives, 18 phenylpropanoids, 13terpenes, 12 phenols, 6 organic acids and its derivatives, 4 alkaloids, etc. Subsequently, 195 key tumorrelated active compounds were identified and established in the Drug-Compound-Target-Disease network. The PPI network screened out 85 key targets (TP53, STAT3, EGFR, GAPDH, BCL2, AKT1, CASP3, mTOR, JUN, and TNF) in tumors. Furthermore, functional enrichment analyses using GO and KEGG pathways highlighted the involvement of PI3K-Akt signaling pathways in S. vaninii's anti-tumor effects. Finally, the top ten significant bioactive constituents were selected as key targets for molecular docking studies which revealed Alpinetin, Galangin, and 4',5-Dihydroxyflavone as potential core compounds targeting mTOR, EGFR, and AKT1 respectively; these complexes were further assessed for stability through MD simulations.</p><p><strong>Conclusion: </strong>This study provides insights into the potential active compounds, target proteins, and signaling pathways underlying the clinical application of S. vaninii in treating tumors.</p>","PeriodicalId":10845,"journal":{"name":"Current pharmaceutical design","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Data-independent Acquisition Mass Spectrometry Reveals Exosomal LAMC1 as a Key Determinant of Lung Adenocarcinoma Radiosensitivity, Independent of EGFR Mutation.","authors":"Chang Jiang, Xueru Zhu, Yiting Wang, Wen Feng, Li Sun, Qian Sun, Hao Jiang, Xiaolong Fu","doi":"10.2174/0113816128392494250430173324","DOIUrl":"https://doi.org/10.2174/0113816128392494250430173324","url":null,"abstract":"<p><strong>Aim: </strong>This study is formulated to reveal the variables affecting the radio-sensitivity in lung adenocarcinoma (LUAD).</p><p><strong>Background: </strong>LUAD patients show varied radiotherapy responses. While epidermal growth factor receptor (EGFR) mutations are often used to predict sensitivity, their reliability is debated, underscoring the need for better biomarkers.</p><p><strong>Objective: </strong>The aim of this study was to identify key functional proteins that regulate the sensitivity of LUAD to radiotherapy and to assess the potential value of exosomal LAMC1 as a clinical predictive marker.</p><p><strong>Method: </strong>In this study, 103 LUAD patients receiving concurrent radiotherapy were included to assess the relationship between EGFR mutation and survival. Intrinsic radio-sensitivity and different radio-sensitivities in 14 LUAD cell lines with/out EGFR mutation were examined based on the surviving fraction at 2 Gy (SF2). Dataindependent acquisition (DIA) and Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics were used to investigate the proteomics of the LUAD cell lines. Subsequently, GO/KEGG enrichment analysis was combined with protein-protein interaction (PPI) network screening for key proteins. Nano-flow cytometry was employed to validate changes in radiosensitivity-associated protein expression within exosomes, while siRNA-mediated knockdown was performed to assess the functional impact of specific proteins on LUAD cells.</p><p><strong>Results: </strong>EGFR mutations were not significantly associated with PFS/OS. 14 LUAD cell lines displayed intrinsic variations in SF2, and no difference between the EGFR mutation and wild-type groups was reported. 5425 proteins were identified via DIA in 14 LUAD cell lines. After bio-informatics analysis, LAMC1, ITGB4, ITGA6, and CD44 were the most representative core differential proteins for the radio-sensitivity in LUAD cells. Notably, LAMC1 was confirmed as a radiation-resistant protein. Following radiotherapy, LUAD cells secreted exosomes with reduced LAMC1 levels. Moreover, LAMC1 knockdown significantly affected cellular proliferation and apoptosis post-irradiation.</p><p><strong>Conclusion: </strong>LAMC1 serves as a critical functional determinant of radiotherapy resistance in LUAD. Its dynamic changes in exosomes demonstrate potential for predicting radiotherapy response, suggesting clinical utility for radiosensitivity assessment and personalized radiotherapy guidance.</p>","PeriodicalId":10845,"journal":{"name":"Current pharmaceutical design","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143982628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxic Effects of Dysphania ambrosioides Extracts on Oral Squamous Cell Carcinoma.","authors":"Alexia Nathália Brígido Assef, Denis Francisco Gonçalves de Oliveira, Khalil Fernandes Viana, Sthefane Gomes Feitosa, Francisco das Chagas Lima Pinto, Thâmara Manoela Marinho Bezerra, Otília Deusdênia Loiola Pessoa, Karuza Maria Alves Pereira","doi":"10.2174/0113816128384050250424053432","DOIUrl":"https://doi.org/10.2174/0113816128384050250424053432","url":null,"abstract":"<p><strong>Introduction: </strong>Dysphania ambrosioides, commonly known as \"mastruz,\" is a medicinal plant traditionally used for its therapeutic properties, including antimicrobial and anti-inflammatory effects. Previous studies have also suggested its antitumor potential. However, its role in oral squamous cell carcinoma (OSCC) remains unexplored. This study aimed to evaluate the in vitro cytotoxic effects of D. ambrosioides extracts on SCC4 (OSCC) and HaCaT (human keratinocyte) cell lines.</p><p><strong>Methods: </strong>Crude extracts were obtained using different methods, including hexanic, ethanolic, hydroethanolic (7:3), and aqueous extractions, all performed ultrasonic-assisted extraction. The extracts were tested at concentrations ranging from 7.81 μg/mL to 1000 μg/mL using 2-fold serial dilutions. Cell viability was assessed after 48 hours of treatment using the MTT assay, with DMSO as the control.</p><p><strong>Results: </strong>The extracts exhibited concentration-dependent cytotoxic effects on both cell lines, with HaCaT cells showing greater sensitivity. However, the lack of selectivity toward tumor cells over normal cells suggests a broad-spectrum cytotoxic activity without tumor-specific therapeutic targeting.</p><p><strong>Conclusion: </strong>These findings highlight the need for further fractionation of the extracts and identification of the bioactive compounds responsible for the observed effects. Although the extracts demonstrated significant cytotoxic activity, their therapeutic potential should not be limited to cytotoxicity alone. Future studies should explore additional biological activities, such as anti-inflammatory or immunomodulatory properties, to fully understand the therapeutic applications of D. ambrosioides.</p>","PeriodicalId":10845,"journal":{"name":"Current pharmaceutical design","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}