Critical Reviews in Biotechnology最新文献_第2页

Therapeutic recombinant L-asparaginases: a comprehensive review and way forward. 治疗性重组l -天冬酰胺酶：全面回顾和前进方向。

IF 8.7 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2026-09-01 Epub Date: 2026-06-05 DOI: 10.1080/07388551.2026.2669312

Subhash Kumar, Virender Kumar, Sanyukta Darnal, Dharam Singh

{"title":"Therapeutic recombinant L-asparaginases: a comprehensive review and way forward.","authors":"Subhash Kumar, Virender Kumar, Sanyukta Darnal, Dharam Singh","doi":"10.1080/07388551.2026.2669312","DOIUrl":"10.1080/07388551.2026.2669312","url":null,"abstract":"L-Asparaginase (E.C.3.5.1.1; L-ASNase) hydrolyzes L-asparagine (an essential amino acid for the growth of leukemic cells) to aspartic acid and ammonia. It is obtained from various sources, including: bacteria, yeast, fungi, plants, and animals. It is used as a chemotherapeutic agent to treat acute lymphoblastic leukemia (ALL) and reduce acrylamide formation in baked and fried foods. Globally, various recombinant and pegylated L-ASNases formulations, including Escherichia coli and Erwinia-derived variants, are in Phase II or active clinical trials for ALL and related conditions across the USA, Brazil, and Canada. Some drugs are recruiting, and other variants of E. coli and E. chrysanthemi L-ASNases are already approved for marketing. Although L-ASNase is used as a therapeutic agent, immunogenic reactions and other adverse effects continue to limit its use. To enhance yield, L-ASNase isoforms are cloned and expressed in host cells, generating recombinants with distinct physicochemical properties and kinetic parameters. The enzymes' temperature and pH ranges vary from 25 to 100 °C and 6 to 10, respectively. Nowadays, enzymatic modifications, such as immobilization (chemical, physical, and PEGylation), mutagenesis, and PASylation, are used to overcome the limitations of commercialized L-ASNase-based drugs. Therefore, this review is a timely effort to compile and analyze the properties of recombinant L-ASNases and the contemporary techniques used to improve L-ASNase. A comprehensive study would help us better understand the kinetic parameters, biochemical properties, and modification trends of L-ASNase, enabling the development of robust, reliable therapeutics in the future.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1037-1060"},"PeriodicalIF":8.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148162014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From discovery to application: cytochrome P450 enzyme in filamentous fungi for biosynthesis of steroids. 从发现到应用：丝状真菌中用于类固醇生物合成的细胞色素P450酶。

IF 8.7 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2026-09-01 Epub Date: 2026-07-21 DOI: 10.1080/07388551.2026.2691230

Guojuan Yi, Xiao Yan, Dongping Yao, Linyu Luo, Jingbo Zhou, Tolbert Osire, Mengfei Long, Yongmei Xie, Guojian Liao

{"title":"From discovery to application: cytochrome P450 enzyme in filamentous fungi for biosynthesis of steroids.","authors":"Guojuan Yi, Xiao Yan, Dongping Yao, Linyu Luo, Jingbo Zhou, Tolbert Osire, Mengfei Long, Yongmei Xie, Guojian Liao","doi":"10.1080/07388551.2026.2691230","DOIUrl":"10.1080/07388551.2026.2691230","url":null,"abstract":"Steroids represent a cornerstone of modern pharmacotherapy, with their physiological functions intrinsically linked to precise hydroxylation patterns on the steroid nucleus. Hydroxylation of inert C-H bonds on the steroid nucleus is critical for their physiological and pharmacological activities. However, conventional chemical hydroxylation often suffers from complex procedures and poor regioselectivity. In contrast, microbial cytochrome P450 enzyme (P450) mediated biocatalysis enables precise, single-step steroid hydroxylation under mild conditions, overcoming the limitations of conventional approaches. Filamentous fungi are particularly notable as prolific hosts for steroid transformation, harboring a vast and underexplored repertoire of P450s with exceptional catalytic diversity. Despite their potential, a comprehensive summary of the identification approaches and application of steroid-hydroxylating P450s from filamentous fungi remains limited. This review provides an in-depth overview of recently identified and engineered P450s from filamentous fungi, emphasizing the integration of multi-omics approaches and computational tools in their discovery. We highlight breakthroughs in protein engineering and metabolic regulation that have enhanced the regio- and stereo-selective capabilities of these enzymes. It establishes a foundation for future investigations into the roles and applications of steroid hydroxylase P450s.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"980-998"},"PeriodicalIF":8.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148548023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable and green novel sorbent-based immobilization of PFAS and their enhanced adsorption to treat environmental contamination. 可持续绿色新型吸附基固定化PFAS及其对环境污染的强化吸附。

IF 8.7 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2026-09-01 Epub Date: 2026-07-01 DOI: 10.1080/07388551.2026.2682347

Rajendra Singh, Hayoung Kim, Minjae Kim, Praveen Kumar Mehta, Keugtae Kim

{"title":"Sustainable and green novel sorbent-based immobilization of PFAS and their enhanced adsorption to treat environmental contamination.","authors":"Rajendra Singh, Hayoung Kim, Minjae Kim, Praveen Kumar Mehta, Keugtae Kim","doi":"10.1080/07388551.2026.2682347","DOIUrl":"10.1080/07388551.2026.2682347","url":null,"abstract":"The persistent environmental contamination resulting from per- and polyfluoroalkyl substances (PFAS) presents substantial challenges to both environment and public health, thereby underscoring the persistent necessity for sustainable remediation approaches. The removal of PFAS from contaminated water using sorbent materials, particularly sustainable and green materials, is a critical area of research due to their inherent biodegradability, biocompatibility, and eco-friendly properties. This review explores recent progress in sustainable and eco-friendly strategies aimed at PFAS immobilization through the creation of novel sorbent materials. By emphasizing the incorporation of biodegradable and nontoxic constituents, we elucidated the improved adsorption efficacy of engineered sorbents for the effective sequestering of PFAS compounds. A thorough examination of various batch experiments indicated that these novel sorbents exhibit enhanced PFAS removal efficiency compared to conventional remediation techniques. Moreover, we highlight the challenges that remain despite the promising attributes of sustainable and eco-friendly sorbents for PFAS removal and deliberate on prospective opportunities for their extensive utilization in the ongoing efforts to combat PFAS pollution.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"939-956"},"PeriodicalIF":8.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148367031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial engineering for the conversion of C1 feedstocks into value-added bioproducts: recent advances and perspectives. 将C1原料转化为增值生物产品的微生物工程：最新进展和展望。

IF 8.7 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2026-09-01 Epub Date: 2026-07-01 DOI: 10.1080/07388551.2026.2683861

Yang Li, Guiping Xu, Yujia Zheng, Mingxiong Liu, Changyang Yang, Hongxin Fu, Jufang Wang

{"title":"Microbial engineering for the conversion of C1 feedstocks into value-added bioproducts: recent advances and perspectives.","authors":"Yang Li, Guiping Xu, Yujia Zheng, Mingxiong Liu, Changyang Yang, Hongxin Fu, Jufang Wang","doi":"10.1080/07388551.2026.2683861","DOIUrl":"10.1080/07388551.2026.2683861","url":null,"abstract":"Biomanufacturing advances sustainable production and circular economy, while wastes-derived one-carbon (C1) compounds (e.g., CO2, methanol, formate and methane) expand substrate diversity, accelerate wastes valorization, and mitigate climate change. The design of C1 feedstocks-driven biorefineries is often hindered by: limited genetic toolkits, impaired cell growth, inefficient substrate utilization, and unclear metabolic mechanism. In this context, this review addresses these challenges through comprehensive analysis of: pathway exploitation, metabolic regulation, emerging technology, and biochemicals synthesis for optimizing C1-trophic performance. We first analyze key bottlenecks in enhancing assimilation efficiency of natural C1-utilizers, and then systematically summarize the strategies for harnessing nontraditional feedstocks using: engineered autotrophs, methylotrophs, formatotrophs, and methanotrophs. Importantly, we outline a bottom-up framework on systematic and modular redesign of C1-driven microbial cell factories for promoting industrial applications. Finally, we identify unresolved challenges and strategic opportunities to guide environmental preservation and performance optimization. Overall, this review provides a roadmap for transformative progress in sustainable biomanufacturing and wastes re-utilization.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"957-979"},"PeriodicalIF":8.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148367418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing natural diversity of Saccharomyces cerevisiae for enhanced resistance to weak acids. 利用酿酒酵母的自然多样性增强对弱酸的抗性。

IF 8.7 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2026-09-01 Epub Date: 2026-07-21 DOI: 10.1080/07388551.2026.2688834

Rebecca My, Laura Corte, Gianluigi Cardinali, Lorenzo Favaro

{"title":"Harnessing natural diversity of Saccharomyces cerevisiae for enhanced resistance to weak acids.","authors":"Rebecca My, Laura Corte, Gianluigi Cardinali, Lorenzo Favaro","doi":"10.1080/07388551.2026.2688834","DOIUrl":"10.1080/07388551.2026.2688834","url":null,"abstract":"The production of second-generation bioethanol from lignocellulosic biomass is a promising solution for sustainable energy, yet it faces significant challenges also due to the inhibitory effects of weak acids released during biomass pretreatment, particularly acetic, formic and levulinic acids. This review describes the ability of Saccharomyces cerevisiae, with a focus on natural isolates, in overcoming these challenging compounds. Indeed, natural isolates exhibit greater genetic and phenotypic diversity than laboratory and industrial strains, offering unique traits such as enhanced stress tolerance, metabolic efficiency, and adaptive responses to weak acids. This investigation explores the transcriptional and genomic mechanisms underlying yeast adaptive responses, emphasizing key regulatory networks and resistance pathways, including drug H+ antiporters, Reactive Oxygen Species (ROS) mitigation strategies, and membrane composition adjustments. Strategies for strains improvement, involving adaptive laboratory evolution (ALE), genome shuffling, and hybridization, are also discussed as complementary approaches to develop robust yeast capable of thriving under stressful industrial fermentation conditions. The integration of these techniques, along with genomic and transcriptomic insights, provides a comprehensive framework for engineering high-performance yeast strains. Ultimately, this review underscores the potential of leveraging natural diversity and innovative biotechnological strategies to advance the scalability and efficiency of lignocellulosic bioethanol production through S. cerevisiae fermentation.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"879-899"},"PeriodicalIF":8.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148548054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances, challenges, and opportunities in engineering C5 and C6 sugar transporters in yeast for bio-based industrial biotechnology. 酵母中C5和C6糖转运体工程在生物基工业生物技术中的进展、挑战和机遇

IF 8.7 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2026-09-01 Epub Date: 2026-07-01 DOI: 10.1080/07388551.2026.2680416

João Gabriel Ribeiro Bueno, Ícaro Fier, Douglas Bruce Kell, Leandro Vieira Dos Santos

{"title":"Advances, challenges, and opportunities in engineering C5 and C6 sugar transporters in yeast for bio-based industrial biotechnology.","authors":"João Gabriel Ribeiro Bueno, Ícaro Fier, Douglas Bruce Kell, Leandro Vieira Dos Santos","doi":"10.1080/07388551.2026.2680416","DOIUrl":"10.1080/07388551.2026.2680416","url":null,"abstract":"Harnessing biomass for bio-based industrial biotechnology is vital for addressing global energy needs and mitigating climate change. In this context, microorganisms are the cornerstone of biorefineries based on renewable materials, with applications in bioenergy, agriculture, biomedicine, and other sectors. By engineering metabolic pathways, microorganisms can be tailored to improve yields, tolerate industrial conditions, and selectively produce valuable compounds. Through advances in metabolic engineering and synthetic biology, engineered strains of the yeast Saccharomyces cerevisiae have been successfully developed to efficiently convert the pentose sugars D-xylose and L-arabinose. Despite this important breakthrough, the efficient transport of these sugars remains a major limitation. Sugar sensing and transport in yeast are regulated at both transcriptional and post-translational levels. D-xylose is not recognized as a fermentable carbon source, leading to downregulation of transporter expression, removal from the cytoplasmic membrane, and degradation via ubiquitination in the absence of extracellular glucose. Additionally, transporters exhibit lower affinity for C5 sugars compared to D-glucose, resulting in strong D-glucose repression. To address these challenges, cutting-edge strategies have been successfully employed, including rational protein engineering, directed evolution, and machine learning approaches, to expand the repertoire of C5 transporters available for engineering in S. cerevisiae. Specific D-xylose transporters have been redesigned, with key residues identified to reduce D-glucose affinity, while studies have demonstrated improvements in transporter stability and sugar uptake rates. This review summarizes the key bottlenecks in C5 sugar transport and highlights the major advances and progress made toward creating robust microbial platforms capable of sustainable and efficient bio-based production.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"920-938"},"PeriodicalIF":8.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148367404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Opportunities in next-generation porous membrane-based extracellular vesicle processing and applications. 下一代多孔膜基细胞外囊泡加工和应用的机遇。

IF 8.7 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2026-08-31 DOI: 10.1080/07388551.2026.2699342

Kevin E Petersen, Munther Alsudais, Mehdi Dehghani, Thomas R Gaborski

{"title":"Opportunities in next-generation porous membrane-based extracellular vesicle processing and applications.","authors":"Kevin E Petersen, Munther Alsudais, Mehdi Dehghani, Thomas R Gaborski","doi":"10.1080/07388551.2026.2699342","DOIUrl":"https://doi.org/10.1080/07388551.2026.2699342","url":null,"abstract":"Extracellular vesicle (EV) research and applications increasingly leverages porous membrane technologies beyond traditional filtration methods used for isolation and purification. While filtration remains foundational in EV handling, emerging applications employ membranes directly in new EV research contexts, such as: three-dimensional cell culture, tissue barrier modeling, environmental monitoring, and horizontal EV transfer studies. Concurrently, advancements in membrane science are providing novel modifications leading to significantly enhanced membrane performance in EV applications. These innovations include: surface-enhanced membranes for plasmonic detection, selective EV corona characterization and manipulation, targeted EV removal strategies, self-assembled and patterned membranes to simplify production and improve consistency, customized pore geometries for optimized throughput, and self-cleaning membranes to mitigate fouling. Collectively, developments in these porous membrane technologies promise to markedly improve the effectiveness, precision, and scope of EV-based research and applications, particularly within diagnostics, therapeutics, and cancer biology.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1-18"},"PeriodicalIF":8.7,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148863773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current trends and advances of microbial biosurfactants in the biomedical and pharmaceutical applications. 微生物表面活性剂在生物医学和制药领域的应用现状及进展。

IF 8.7 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2026-08-13 DOI: 10.1080/07388551.2026.2711971

Raman Krishnamoorthi, Pambayan Ulagan Mahalingam, Kaviyadharshini Muthuramalingam, Shu-Han Chang, Cheng-Yu Lin, Jia-You Fang

{"title":"Current trends and advances of microbial biosurfactants in the biomedical and pharmaceutical applications.","authors":"Raman Krishnamoorthi, Pambayan Ulagan Mahalingam, Kaviyadharshini Muthuramalingam, Shu-Han Chang, Cheng-Yu Lin, Jia-You Fang","doi":"10.1080/07388551.2026.2711971","DOIUrl":"https://doi.org/10.1080/07388551.2026.2711971","url":null,"abstract":"Microbial biosurfactants are emerging as sustainable alternatives to synthetic surfactants due to their biodegradability, low toxicity, and multifunctional biological activities. Their potential in biomedical and pharmaceutical applications is increasingly recognized, yet integrated evaluations that link production strategies to translational outcomes remain limited. This review critically examines recent advances in biosurfactant production, optimization, and purification, as well as their applications in drug delivery, nanomedicine, antimicrobial therapy, vaccine formulation, wound healing, and immunomodulation. Major classes, including glycolipids, lipopeptides, phospholipids, and polymeric biosurfactants, are compared in terms of physicochemical properties and therapeutic potential. Evidence shows biosurfactants can reduce bacterial adhesion by up to 90%, inhibit biofilm formation by over 80%, and enhance antibiotic efficacy against multidrug-resistant pathogens. Biosurfactant-based carriers such as liposomes, micelles, nanoemulsions, and nanoparticles improve drug solubility, stability, bioavailability, and targeted release. Advances in engineered microbial platforms, low-cost substrates, and process optimization have enhanced production feasibility, though clinical translation remains constrained by cost, downstream processing, formulation stability, safety, and regulatory hurdles. Emerging approaches, including AI-assisted optimization and multiomics-guided discovery, promise next-generation biosurfactants with superior therapeutic performance.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1-26"},"PeriodicalIF":8.7,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148760039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzyme-driven antimicrobial resistance and advancements in sustainable anti-infective strategies. 酶驱动的抗菌素耐药性和可持续抗感染策略的进展。

IF 8.7 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2026-08-13 DOI: 10.1080/07388551.2026.2693159

Ananya Mukherjee, Damini Thakur, Maira Ahuja, Pooskuri Nayan, Lokender Kumar

{"title":"Enzyme-driven antimicrobial resistance and advancements in sustainable anti-infective strategies.","authors":"Ananya Mukherjee, Damini Thakur, Maira Ahuja, Pooskuri Nayan, Lokender Kumar","doi":"10.1080/07388551.2026.2693159","DOIUrl":"https://doi.org/10.1080/07388551.2026.2693159","url":null,"abstract":"Antibiotic resistance is an emerging global issue that has reduced the efficacy of antibiotics for treating life-threatening bacterial infections. Bacterial adaptive enzymatic defense mechanisms allow cells to activate or modify specific enzymes that inactivate antibiotics and support survival under antimicrobial stress. Antibiotics are predominantly inactivated through enzymatic degradation or chemical modification. Most of the β-lactamases, macrolide esterases, tetracycline-modifying enzymes, fosfomycin degrading enzymes, aminoglycoside-modifying enzymes, and other bacterial enzymes chemically modify or degrade the antibiotics making them inactive. This review covers classification and mechanisms of enzyme-mediated resistance and emphasizes the significant enzymes involved in inactivation of various antibiotic classes. It also summarizes recent biotechnological advances to combat antibiotic resistance, including β-lactamase inhibitors, phage therapy, antimicrobial peptides, and CRISPR-Cas9 systems, along with emerging therapeutic approaches and current trends in antibiotic research. A deeper understanding of enzyme-mediated resistance and the cellular intelligence driving bacterial adaptation is crucial for designing effective therapeutic strategies, preserving antibiotic efficacy, and reducing the global burden of resistant infections.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1-39"},"PeriodicalIF":8.7,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances and perspectives in multi-enzyme systems and immobilization for efficient rare sugars synthesis. 多酶系统和固定化高效合成稀有糖的研究进展与展望。

IF 8.7 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2026-08-13 DOI: 10.1080/07388551.2026.2711978

Kangqing Fei, Zijie Li

{"title":"Recent advances and perspectives in multi-enzyme systems and immobilization for efficient rare sugars synthesis.","authors":"Kangqing Fei, Zijie Li","doi":"10.1080/07388551.2026.2711978","DOIUrl":"https://doi.org/10.1080/07388551.2026.2711978","url":null,"abstract":"Rare sugars have attracted substantial interest owing to their critical roles in agriculture, cosmetics, food, and pharmaceutical industries. Traditional chemical synthesis methods face limitations such as low selectivity and environmental concerns. Consequently, enzymatic synthesis of rare sugars has emerged as an increasingly prominent research focus. To address the limitations of single-enzyme systems, multi-enzyme reactions have been developed as promising approaches, encompassing isomerases, epimerases, aldolases, phosphatases, oxidases, dehydratases, and associated coenzymes. Through the utilization of multi-enzyme systems, substrate costs can be reduced while the overall yields of rare sugars are enhanced. Furthermore, the application of immobilized enzymes is critical for optimizing the production process. Immobilization techniques can reduce reaction costs, improve enzyme properties, and enhance efficiency and stability. This review summarizes multi-enzyme systems for rare sugars biosynthesis and diverse immobilization methods, including traditional approaches, innovative nanomaterial-based techniques, and the use of scaffold proteins. Additionally, this review incorporates a techno-economic analysis of rare sugars production, addressing bottlenecks associated with enzyme stability and process costs, aiming to provide guidance for the industrial translation of rare sugar manufacturing technologies.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1-18"},"PeriodicalIF":8.7,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0