Critical Reviews in Biotechnology最新文献

{"title":"Regulation of fatty acid synthesis in oilseed crops: multidimensional insights and strategies for enhancing oil quality.","authors":"Qian Zhao, Fu Wang, Aisheng Xiong, Shuyao Li, Yingping Wang, Xiujuan Lei, Michael K Deyholos, Yahui Wang, Jing Zhuang, Hansong Yu, Jian Zhang","doi":"10.1080/07388551.2025.2529591","DOIUrl":"https://doi.org/10.1080/07388551.2025.2529591","url":null,"abstract":"<p><p>The adage \"Food is the God of the people\" underscores the profound interconnectedness between agriculture and the food industry. Agriculture forms the backbone of the food industry, while evolving consumer preferences continuously shape its progress. The balance between saturated and unsaturated fatty acids (SFAs and UFAs) in vegetable oils is critical to human health. As health awareness grows, UFAs have gained significant market traction, prompting extensive research into their biosynthesis, regulation, and improvement. This review focuses on oilseed crops, offering a comprehensive analysis of: fatty acid composition, biosynthesis pathways, gene regulation, and breeding strategies to enhance quality. By integrating theoretical and practical insights, our work aims to provide guidance for promoting sustainable agriculture and advancing the food industry.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1-17"},"PeriodicalIF":7.7,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144945848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the silent defenders: mycobacterial stress sensors at the forefront to combat tuberculosis.","authors":"Manya Jain, Rajan Vyas","doi":"10.1080/07388551.2024.2449367","DOIUrl":"10.1080/07388551.2024.2449367","url":null,"abstract":"<p><p>The global escalation in tuberculosis (TB) cases accompanied by the emergence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains of <i>Mycobacterium tuberculosis</i> (<i>M.tb)</i> emphasizes the critical requirement for novel potent drugs. The <i>M.tb</i> demonstrates extraordinary adaptability, thriving in diverse conditions, and always finds itself in win-win situations regardless of whether the environment is favorable or unfavorable; no matter the magnitude of the challenge, it can endure and survive. This review aims to uncover the role of multiple stress sensors of <i>M.tb</i> that assist bacteria in remaining viable within the host for years against various physiological stresses offered by the host. <i>M.tb</i> is an exceptionally triumphant pathogen, primarily due to its adeptness in developing defense mechanisms against stressful situations. The recent advances emphasize the significance of <i>M.tb</i> stress sensors, including chaperones, proteases, transcription factors, riboswitches, inteins, etc., employed in responding to a spectrum of physiological stresses imposed by the host, encompassing surface stress, host immune responses, osmotic stress, oxidative and nitrosative stresses, cell envelope stress, environmental stress, reductive stress, and drug pressure. These sensors act as silent defenders orchestrating adaptive strategies, with limited comprehensive information in current literature, necessitating a focused review. The <i>M.tb</i> strategies utilizing these stress sensors to mitigate the impact of traumatic conditions demand attention to neutralize this pathogen effectively. Moreover, the intricacies of these stress sensors provide potential targets to design an effective TB drug using structure-based drug design against this formidable global health threat.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1329-1347"},"PeriodicalIF":7.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endosymbionts as hidden players in tripartite pathosystem of interactions and potential candidates for sustainable viral disease management.","authors":"Muhammad Dilshad Hussain, Tahir Farooq, Ali Kamran, Abdul Basit, Yong Wang, Guy Smagghe, Xiangru Chen","doi":"10.1080/07388551.2024.2449403","DOIUrl":"10.1080/07388551.2024.2449403","url":null,"abstract":"<p><p>The convoluted relationships between plants, viruses, and arthropod vectors housing bacterial endosymbionts are pivotal in the spread of harmful plant viral diseases. Endosymbionts play key roles in: manipulating host responses, influencing insect resistance to pesticides, shaping insect evolution, and bolstering virus acquisition, retention, and transmission. This interplay presents an innovative approach for developing sustainable strategies to manage plant diseases. Recent progress in targeting specific endosymbionts through genetic modifications, biotechnological advancements, and RNA interference shows potential for curbing viral spread and disease progression. Additionally, employing synthetic biology techniques like CRISPR/Cas9 to engineer endosymbionts and disrupt crucial interactions necessary for viral transmission in arthropod vectors holds promise for effective control measures. In this review, these obligate and facultative bacterial cruxes have been discussed to elaborate on their mechanistic involvement in the regulation and/or inhibition of tripartite pathways of interactions. Furthermore, we provide an in-depth understanding of endosymbionts' synergistic and antagonistic effects on: insect biology, plant immunity, and virus acquisition and transmission. Finally, we point out open questions for future research and provide research directions concerning the deployment of genetically engineered symbionts to affect plant-virus-vector interactions for sustainable disease management. By addressing existing knowledge gaps and charting future research paths, a deeper comprehension of the role of endosymbionts in plant-virus-vector interactions can pave the way for innovative and successful disease management strategies. The exploration of antiviral therapies, paratransgenesis, and pathogen-blocking tactics using engineered endosymbionts introduces pioneering solutions for lessening the impact of plant viral diseases and green pest management.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1348-1370"},"PeriodicalIF":7.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing microbial division of labor for biomanufacturing: a review of laboratory and formal modeling approaches.","authors":"Fatima Ceballos Rodriguez-Conde, Sophie Zhu, Duygu Dikicioglu","doi":"10.1080/07388551.2025.2455607","DOIUrl":"10.1080/07388551.2025.2455607","url":null,"abstract":"<p><p>Bioprocess industries aim to meet the increasing demand for product complexity by designing enhanced cellular and metabolic capabilities for the host. Monocultures, standard biomanufacturing workhorses, are often restricted in their capability to meet these demands, and the solution often involves the genetic modification of the host. Synthetic microbial communities are a promising alternative to monocultures because they exhibit division of labor, enabling efficient resource utilization and pathway modularity. This specialization minimizes metabolic burden and enhances robustness to perturbations, providing a competitive advantage. Despite this potential, their utilization in biotechnological or bioprocessing applications remains limited. The recent emergence of new and innovative community design tools and strategies, particularly those harnessing the division of labor, holds promise to change this outlook. Understanding the microbial interactions governing natural microbial communities can be used to identify complementary partners, informing synthetic community design. Therefore, we particularly consider engineering division of labor in synthetic microbial communities as a viable solution to accelerate progress in the field. This review presents the current understanding of how microbial interactions enable division of labor and how this information can be used to design synthetic microbial communities to perform tasks otherwise unfeasible to individual organisms. We then evaluate laboratory and formal modeling approaches specifically developed to: elucidate microbial community physiology, guide experimental design, and improve our understanding of complex community interactions assisting synthetic community design. By synthesizing these insights, we aim to present a comprehensive framework that advances the use of microbial communities in biomanufacturing applications.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1249-1267"},"PeriodicalIF":7.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143457190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> cellular model systems provide a promising alternative to animal experiments for studying the intestine-organ axis.","authors":"Alessandra Vitale, Cristiana De Musis, Marida Bimonte, Josep Rubert, Vincenzo Fogliano","doi":"10.1080/07388551.2025.2452620","DOIUrl":"10.1080/07388551.2025.2452620","url":null,"abstract":"<p><p>Limiting animal experiments is essential for ethical issues and also because scientific evidence highlights the discrepancies between human and animal metabolism. This review aims to provide a critical discussion of the strengths and limitations of the most appropriate <i>in vitro</i> intestine model to answer complex research questions in pharmaceutical and nutraceutical fields. This review describes the components contributing to the definition of the gut barrier structure, from the outer mucus layer to the inner part of lamina propria, including endothelial and neuronal networks. We conclude that the main advantage of these co-culture models is their versatility since they are modulable systems in which each component can be added, changed, or removed to reproduce a specific physiological condition each time. Additionally, we compare intestinal organoid models and microfluidic systems with well-established co-culture models.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1189-1206"},"PeriodicalIF":7.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold-active enzymes from deep marine psychrophiles: harnessing their potential in enhanced food production and sustainability.","authors":"Mrinmoy Ghosh, Yunji Heo, Krishna Kanth Pulicherla, Min Woo Ha, Kyoungtag Do, Young-Ok Son","doi":"10.1080/07388551.2024.2435974","DOIUrl":"10.1080/07388551.2024.2435974","url":null,"abstract":"<p><p>Exploring the untapped potential of deep-sea microorganisms, particularly their cold-active enzymes, or psychrozymes, offers exciting possibilities for revolutionizing various aspects of the food processing industry. This review focuses on these enzymes, derived from the largely unexplored depths of the deep ocean, where microorganisms have developed unique adaptations to extreme conditions. Psychrozymes, as bioactive molecules, hold significant promise for food industry applications. However, despite their potential, the understanding and industrial utilization of psychrozymes remains limited. This review provides an in-depth analysis of how psychrozymes can: improve processing efficiency, enhance sensory qualities, extend product shelf life, and reduce energy consumption across the food production chain. We explore the cryodefense strategies and cold-adaptation mechanisms that support these enzymes, shedding light on the most extensively studied psychrozymes and assessing their journey from theoretical applications to practical use in food production. The key properties, such as stability, substrate specificity, and catalytic efficiency in cold environments, are also discussed. Although psychrozymes show considerable promise, their large-scale application in the food industry remains largely unexplored. This review emphasizes the need for further research to unlock the full potential of psychrozymes, encouraging their broader integration into the food sector to contribute to more sustainable food production processes.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1207-1231"},"PeriodicalIF":7.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the potential of dendrobine: insights into bioproduction, bioactivities, safety, circular economy, and future prospects.","authors":"Surendra Sarsaiya, Archana Jain, Fuxing Shu, Qi Jia, Qihai Gong, Qin Wu, Jingshan Shi, Jishuang Chen","doi":"10.1080/07388551.2024.2438161","DOIUrl":"10.1080/07388551.2024.2438161","url":null,"abstract":"<p><p>This comprehensive review aims to explore and consolidate the current knowledge on dendrobine, including its biological activities, molecular mechanisms of action, effects on various physiological processes, potential toxicity, and safety considerations, in order to unlock its full potential in various applications. Dendrobine has diverse biological effects, including anti-inflammatory, antioxidant, neuroprotective, immunomodulatory, and anticancer effects. Dendrobine also exerts neuroprotective effects by boosting neuronal survival, reducing neuroinflammation, and regulating neurotransmitter release. Dendrobine also has immunomodulatory effects that control immune cell function and modify immunological responses. Dendrobine also exhibits anticancer properties by decreasing tumor angiogenesis and metastasis, causing apoptosis in cancer cells, and inhibiting cancer cell growth. This review also discusses the idea of a circular economy to produce dendrobine, researching cutting-edge and sustainable methods that maximize resource efficiency and reduce environmental effects. Additionally, by supporting circular economy principles for sustainable production and outlining potential future study areas, this discovery will help maximize the use of dendrobine in a variety of applications while preserving its safety and the environment. Future dendrobine research should focus on advancing its application in personalized medicine, exploring green bioproduction methods, and ensuring ethical sourcing practices. An enhanced understanding of its molecular mechanisms and the potential for synergistic therapies could revolutionize healthcare, whereas sustainable production methods and conservation efforts could safeguard its ecological impact.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":"45 6","pages":"1268-1286"},"PeriodicalIF":7.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144910566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress of silk fibroin biomaterial use in oral tissue regeneration engineering.","authors":"Xiao-Qing Qian, Meng Zhang, Hai-Yan Wang","doi":"10.1080/07388551.2025.2472621","DOIUrl":"10.1080/07388551.2025.2472621","url":null,"abstract":"<p><p>The field of tissue engineering has introduced novel prospects for the regeneration of oral tissues, wherein stent materials assume a pivotal role and have garnered increasing attention. As a natural protein with good biocompatibility and adjustable biodegradability, an increasing number of studies focus on the uses of silk fibroin (SF) biomaterials for medical tissue regeneration engineering. Solid evidence has been found for using SF biomaterials in various oral tissue regeneration fields, from endodontics and periodontics to regenerating the maxillofacial bone. In order to provide researchers with a systematic understanding of the application of SF biomaterials to oral tissue regeneration, the present work reviews in detail the common forms of SF biomaterials for oral tissue regeneration as well as their preparation methods. In addition, the common additives used in the corresponding materials are introduced.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1232-1248"},"PeriodicalIF":7.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing bioactive nanocurcumin and curcumin nanocomposites to combat microbial pathogens: a comprehensive review.","authors":"Mahendra Rai, Chistiane M Feitosa, Avinash P Ingle, Patrycja Golinska","doi":"10.1080/07388551.2025.2458006","DOIUrl":"10.1080/07388551.2025.2458006","url":null,"abstract":"<p><p>The alarming rise in bacterial infections including those caused by multidrug-resistant pathogens has garnered the attention of the scientific community, compelling them to explore as novel and effective alternatives to combat these infections. Moreover, the emerging viruses such as Influenza A virus subtype H1N1 (A/H1N1), Middle East Respiratory Syndrome Coronavirus (MERS-CoV), Ebolavirus, recent coronavirus (SARS-CoV-2), etc. also has a significant impact all over the world. Therefore, the management of all such infections without any side effects is one of the most important challenges for the scientific community. Hence, the development of novel and effective antimicrobial agents is a need of the hour. In this context, <i>Curcuma longa</i>, commonly known as turmeric, has been used as traditional medicine for centuries to manage and treat such infections. Its bioactive constituent, curcumin has garnered significant attention in medicine due to its multifunctional bioactivities. Apart from antimicrobial properties, it also possesses potent antioxidant and anti-inflammatory activities. However, available reports suggest that its low solubility, stability, and biocompatibility limit its use. Moreover, on the other hand, it has been reported that these limitations associated with the use of curcumin can be resolved by transforming it into its nano-form, specifically curcumin nanoparticles. Recent advancements have brought curcumin nanoparticles into the spotlight, showcasing superior properties and a broad spectrum of antimicrobial applications. In this review, we have mainly focused on antimicrobial potential of curcumin and nanocurcumin, mechanisms underpinning their antimicrobial actions. Moreover, other aspects of toxicity and safety guidelines for nano-based products have been also discussed.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1306-1328"},"PeriodicalIF":7.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143467132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of non-genetically modified or native pentose fermenting microorganisms in establishing viable lignocellulosic biorefineries in the Brazilian context.","authors":"Thais S Milessi, Juliana P Sandri, Priscila V Arruda, Tayrone D Esteves, Luisa P Pinheiro, Vinod Kumar, Anuj K Chandel","doi":"10.1080/07388551.2025.2452628","DOIUrl":"10.1080/07388551.2025.2452628","url":null,"abstract":"<p><p>Brazil can play a pivotal role in the development of a circular bioeconomy as the country ranks among the top five major agricultural countries in the world producing a foreseeable lignocellulosic biomass from crops, such as sugarcane, soybean, corn, rice, coffee, and eucalyptus. Considering that pentose sugars (C5 sugars) represent 20%-35% of the amount of lignocellulosic biomass components, these sugars have a great potential in the development of carbon neutral economy. From the biomass conversion economic point of view, the conversion of hemicellulose into renewable products with a satisfactory yield is the most needed. However, the biochemical conversion of pentose sugars is challenging due to the scarcity of native pentose sugars fermenting microorganisms. While recent advances in metabolic engineering have been effective in developing a strong molecular chassis for efficient pentose sugars conversion, the yields, productivities, and stability of the genetically modified organisms (GMOs) are major limiting factors for industrial-scale applications. Native lignocellulosic sugars fermenting microorganisms are competent, robust, and inhibitor-tolerant but their lower productivities continue to be a big concern. This article explains the inherent characteristics of native pentose fermenting microorganisms in establishing viable lignocellulosic biorefineries in the Brazilian context, with a special focus on their isolation from Brazilian biodiversity, along with the evaluation of nongenetic engineering techniques to improve strains for biorefinery application.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1287-1305"},"PeriodicalIF":7.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143467145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}