Critical Reviews in Biotechnology最新文献_第2页

Pilot scale polyhydroxyalkanoates biopolymer production using pure cultures: current status and future opportunities. 利用纯培养物生产中试规模的聚羟基烷酸酯生物聚合物：现状与未来机遇。

IF 8.1 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2024-10-20 DOI: 10.1080/07388551.2024.2409112

Phavit Wongsirichot

{"title":"Pilot scale polyhydroxyalkanoates biopolymer production using pure cultures: current status and future opportunities.","authors":"Phavit Wongsirichot","doi":"10.1080/07388551.2024.2409112","DOIUrl":"https://doi.org/10.1080/07388551.2024.2409112","url":null,"abstract":"The development and commercialization of bio-based and biodegradable polyhydroxyalkanoates (PHAs) biopolymers could be crucial for the transition toward a sustainable circular economy. However, despite potential traditional and novel applications in the packaging, textiles, agriculture, automotive, electronics, and biomedical industries, the commercialization of PHAs is limited by their current market competitiveness. This review provides the first critical assessment of the current pure culture pilot-scale PHA literature, which could be crucial in translating promising laboratory-scale developments into industrial-scale commercial PHA production. It will also complement reviews of mixed microbial cultures currently dominating pilot-scale PHA literature. Pure culture fermentations could provide advantages, such as ease of characterizing microbial producers' behavior, higher PHA productivities, and better alignment with existing PHA commercialization and industrial biotechnology approaches. Key aspects, including producer organisms, fermentation volumes and schemes, control schemes, optimization, and properties of the polymers produced, are discussed in-depth, to elucidate important trends, achievements, and knowledge gaps. Furthermore, specific ways for future pilot-scale studies to help address current PHA commercialization challenges are also identified. The insights, and recommendations provided will be extremely beneficial for the future development of PHA production, at both pilot and commercial scales, whilst also being beneficial to the production of other microbial polymers and industrial biotechnology as a whole.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":null,"pages":null},"PeriodicalIF":8.1,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Astaxanthin biosynthesis for functional food development and space missions. 用于功能性食品开发和太空任务的虾青素生物合成。

IF 8.1 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2024-10-20 DOI: 10.1080/07388551.2024.2410364

Xiulan Xie, Moyu Zhong, Xinxin Huang, Xinrui Yuan, Nasser Mahna, Cassamo Ussemane Mussagy, Maozhi Ren

{"title":"Astaxanthin biosynthesis for functional food development and space missions.","authors":"Xiulan Xie, Moyu Zhong, Xinxin Huang, Xinrui Yuan, Nasser Mahna, Cassamo Ussemane Mussagy, Maozhi Ren","doi":"10.1080/07388551.2024.2410364","DOIUrl":"https://doi.org/10.1080/07388551.2024.2410364","url":null,"abstract":"Astaxanthin (AXT), a natural carotenoid, has strong antioxidant and anti-ageing effects and can reduce ultraviolet light-induced damage to cells and DNA, stimulate the immune system, and improve cardiovascular disease prognosis. Despite its wide applications in the: nutraceutical, cosmetic, aquaculture, and pharmaceutical industries, AXT industrial production and application are hindered by natural source scarcity, low production efficiency, and high requirements. This review compares the qualitative differences of AXT derived from different natural sources, evaluates the upstream procedures for AXT expression in different chassis organisms, and investigates synthetic biology- and cell factory-based strategies for the industrial production of natural AXT. Synthetic biology is a promising novel strategy for reprogramming plants or microorganisms to produce AXT. Additionally, genetic engineering using cell factories extends beyond terrestrial applications, as it may contribute to the long-term sustainability of human health during space exploration and migration endeavors. This review provides a theoretical basis for the efficient and accurate genetic engineering of AXT from the microalga Haematococcuspluvialis, providing a valuable reference for future research on the biomanufacturing of AXT and other biological metabolites.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":null,"pages":null},"PeriodicalIF":8.1,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amplification-free nucleic acids detection with next-generation CRISPR/dx systems. 利用新一代 CRISPR/dx 系统进行无扩增核酸检测。

IF 8.1 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2024-09-22 DOI: 10.1080/07388551.2024.2399560

Cia-Hin Lau, Siping Huang, Haibao Zhu

{"title":"Amplification-free nucleic acids detection with next-generation CRISPR/dx systems.","authors":"Cia-Hin Lau, Siping Huang, Haibao Zhu","doi":"10.1080/07388551.2024.2399560","DOIUrl":"https://doi.org/10.1080/07388551.2024.2399560","url":null,"abstract":"CRISPR-based diagnostics (CRISPR/Dx) have revolutionized the field of molecular diagnostics. It enables home self-test, field-deployable, and point-of-care testing (POCT). Despite the great potential of CRISPR/Dx in diagnoses of biologically complex diseases, preamplification of the template often is required for the sensitive detection of low-abundance nucleic acids. Various amplification-free CRISPR/Dx systems were recently developed to enhance signal detection at sufficient sensitivity. Broadly, these amplification-free CRISPR/Dx systems are classified into five groups depending on the signal enhancement strategies employed: CRISPR/Cas12a and/or CRISPR/Cas13a are integrated with: (1) other catalytic enzymes (Cas14a, Csm6, Argonaute, duplex-specific nuclease, nanozyme, or T7 exonuclease), (2) rational-designed oligonucleotides (multivalent aptamer, tetrahedral DNA framework, RNA G-quadruplexes, DNA roller machine, switchable-caged guide RNA, hybrid locked RNA/DNA probe, hybridized cascade probe, or \"U\" rich stem-loop RNA), (3) nanomaterials (nanophotonic structure, gold nanoparticle, micromotor, or microbeads), (4) electrochemical and piezoelectric plate biosensors (SERS nanoprobes, graphene field-effect transistor, redox probe, or primer exchange reaction), or (5) cutting-edge detection technology platforms (digital bioanalysis, droplet microfluidic, smartphone camera, or single nanoparticle counting). Herein, we critically discuss the advances, pitfalls and future perspectives for these amplification-free CRISPR/Dx systems in nucleic acids detection. The continued refinement of these CRISPR/Dx systems will pave the road for rapid, cost-effective, ultrasensitive, and ultraspecific on-site detection without resorting to target amplification, with the ultimate goal of establishing CRISPR/Dx as the paragon of diagnostics.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":null,"pages":null},"PeriodicalIF":8.1,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142281514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential anti-aging applications of microbial-derived surfactantsin cosmetic formulations. 微生物衍生表面活性剂在化妆品配方中的潜在抗衰老应用。

IF 9 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2024-09-18 DOI: 10.1080/07388551.2024.2393420

Amir Mohammad Bagheri,Masoud Mirzahashemi,Soodeh Salarpour,Yasmin Dehghnnoudeh,Ibrahim M Banat,Mandana Ohadi,Gholamreza Dehghannoudeh

{"title":"Potential anti-aging applications of microbial-derived surfactantsin cosmetic formulations.","authors":"Amir Mohammad Bagheri,Masoud Mirzahashemi,Soodeh Salarpour,Yasmin Dehghnnoudeh,Ibrahim M Banat,Mandana Ohadi,Gholamreza Dehghannoudeh","doi":"10.1080/07388551.2024.2393420","DOIUrl":"https://doi.org/10.1080/07388551.2024.2393420","url":null,"abstract":"The skin aging process is a complex interaction of genetic, epigenetic, and environmental factors, such as chemical pollution and UV radiation. There is growing evidence that biosurfactants, especially those of microbial origin, have distinct age-supportive effects through different mechanisms, such as stimulation of fibroblast growth, high antioxidant capacities, and favorable anti-inflammatory properties. With a growing financial contribution of more than 15 m€per year, microbial surfactants (MSs) display unique biological effects on the skin including improved cell mobility, better nutrient access, and facilitated cellular growth under harsh conditions. Their biodegradable nature, unusual surface activity, good safety profile and tolerance to high temperature and pH variations widen their potential spectrum in biomedical and pharmaceutical applications. MSs typically have lower critical micelle concentration (CMC) levels than chemical surfactants enhancing their effectiveness. As natural surfactants, MSs are considered possible \"green\" alternatives to synthetic surfactants with better biodegradability, sustainability, and beneficial functional properties. This review therefore aims to explore the potential impacts of MSs as anti-aging ingredients.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":null,"pages":null},"PeriodicalIF":9.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142268669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in the development of phage-mediated cyanobacterial cell lysis. 噬菌体介导的蓝藻细胞裂解的研发进展。

IF 8.1 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2024-09-16 DOI: 10.1080/07388551.2024.2399530

Haojie Jin, Wanzhao Ge, Mengzhe Li, Yan Wang, Yanjing Jiang, Jiaqi Zhang, Yike Jing, Yigang Tong, Yujie Fu

{"title":"Advances in the development of phage-mediated cyanobacterial cell lysis.","authors":"Haojie Jin, Wanzhao Ge, Mengzhe Li, Yan Wang, Yanjing Jiang, Jiaqi Zhang, Yike Jing, Yigang Tong, Yujie Fu","doi":"10.1080/07388551.2024.2399530","DOIUrl":"https://doi.org/10.1080/07388551.2024.2399530","url":null,"abstract":"Cyanobacteria, the only oxygenic photoautotrophs among prokaryotes, are developing as both carbon building blocks and energetic self-supported chassis for the generation of various bioproducts. However, one of the challenges to optimize it as a more sustainable platform is how to release intracellular bioproducts for an easier downstream biorefinery process. To date, the major method used for cyanobacterial cell lysis is based on mechanical force, which is energy-intensive and economically unsustainable. Phage-mediated bacterial cell lysis is species-specific and highly efficient and can be conducted under mild conditions; therefore, it has been intensively studied as a bacterial cell lysis weapon. In contrast to heterotrophic bacteria, biological cell lysis studies in cyanobacteria are lagging behind. In this study, we reviewed cyanobacterial cell envelope features that could affect cell strength and elicited a thorough presentation of the necessary phage lysin components for efficient cell lysis. We then summarized all bioengineering manipulated pipelines for lysin component optimization and further revealed the challenges for each intent-oriented application in cyanobacterial cell lysis. In addition to applied biotechnology usage, the significance of phage-mediated cyanobacterial cell lysis could also advance sophisticated biochemical studies and promote biocontrol of toxic cyanobacteria blooms.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":null,"pages":null},"PeriodicalIF":8.1,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142281513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in microbial production of geraniol: from metabolic engineering to potential industrial applications. 微生物生产香叶醇的进展：从代谢工程到潜在的工业应用。

IF 9 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2024-09-12 DOI: 10.1080/07388551.2024.2391881

Xun Wang,Xinyi Zhang,Jia Zhang,Yujunjie Zhou,Fei Wang,Zhiguo Wang,Xun Li

{"title":"Advances in microbial production of geraniol: from metabolic engineering to potential industrial applications.","authors":"Xun Wang,Xinyi Zhang,Jia Zhang,Yujunjie Zhou,Fei Wang,Zhiguo Wang,Xun Li","doi":"10.1080/07388551.2024.2391881","DOIUrl":"https://doi.org/10.1080/07388551.2024.2391881","url":null,"abstract":"Geraniol, an acyclic monoterpene alcohol, has significant potential applications in various fields, including: food, cosmetics, biofuels, and pharmaceuticals. However, the current sources of geraniol mainly include plant tissue extraction or chemical synthesis, which are unsustainable and suffer severely from high energy consumption and severe environmental problems. The process of microbial production of geraniol has recently undergone vigorous development. Particularly, the sustainable construction of recombinant Escherichia coli (13.2 g/L) and Saccharomyces cerevisiae (5.5 g/L) laid a solid foundation for the microbial production of geraniol. In this review, recent advances in the development of geraniol-producing strains, including: metabolic pathway construction, key enzyme improvement, genetic modification strategies, and cytotoxicity alleviation, are critically summarized. Furthermore, the key challenges in scaling up geraniol production and future perspectives for the development of robust geraniol-producing strains are suggested. This review provides theoretical guidance for the industrial production of geraniol using microbial cell factories.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":null,"pages":null},"PeriodicalIF":9.0,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142252375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in metabolic engineering for enhanced acetyl-CoA availability in yeast. 提高酵母乙酰-CoA 利用率的代谢工程研究进展。

IF 9 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2024-09-12 DOI: 10.1080/07388551.2024.2399542

Yuanyuan Sha,Mianshen Ge,Minrui Lu,Zhaoxian Xu,Rui Zhai,Mingjie Jin

{"title":"Advances in metabolic engineering for enhanced acetyl-CoA availability in yeast.","authors":"Yuanyuan Sha,Mianshen Ge,Minrui Lu,Zhaoxian Xu,Rui Zhai,Mingjie Jin","doi":"10.1080/07388551.2024.2399542","DOIUrl":"https://doi.org/10.1080/07388551.2024.2399542","url":null,"abstract":"Acetyl-CoA is an intermediate metabolite in cellular central metabolism. It's a precursor for various valuable commercial products, including: terpenoids, fatty acids, and polyketides. With the advancement of metabolic and synthetic biology tools, microbial cell factories have been constructed for the efficient synthesis of acetyl-CoA and derivatives, with the Saccharomyces cerevisiae and Yarrowia lipolytica as two prominent chassis. This review summarized the recent developments in the biosynthetic pathways and metabolic engineering approaches for acetyl-CoA and its derivatives synthesis in these two yeasts. First, the metabolic routes involved in the biosynthesis of acetyl-CoA and derived products were outlined. Then, the advancements in metabolic engineering strategies for channeling acetyl-CoA toward the desired products were summarized, with particular emphasis on: enhancing metabolic flux in different organelles, refining precursor CoA synthesis, optimizing substrate utilization, and modifying protein acetylation level. Finally, future developments in advancing the metabolic engineering strategies for acetyl-CoA and related derivatives synthesis, including: reducing CO2 emissions, dynamically regulating metabolic pathways, and exploring the regulatory functions between acetyl-CoA levels and protein acetylation, are highlighted. This review provided new insights into regulating acetyl-CoA synthesis to create more effective microbial cell factories for bio-manufacturing.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":null,"pages":null},"PeriodicalIF":9.0,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142252374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of C-N bonds by nicotinamide-dependent oxidoreductase: an overview. 烟酰胺依赖性氧化还原酶合成 C-N 键：概述。

IF 8.1 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2024-09-04 DOI: 10.1080/07388551.2024.2390082

Tianfu Wu, Wanqing Wei, Changzheng Gao, Jing Wu, Cong Gao, Xiulai Chen, Liming Liu, Wei Song

{"title":"Synthesis of C-N bonds by nicotinamide-dependent oxidoreductase: an overview.","authors":"Tianfu Wu, Wanqing Wei, Changzheng Gao, Jing Wu, Cong Gao, Xiulai Chen, Liming Liu, Wei Song","doi":"10.1080/07388551.2024.2390082","DOIUrl":"https://doi.org/10.1080/07388551.2024.2390082","url":null,"abstract":"Compounds containing chiral C-N bonds play a vital role in the composition of biologically active natural products and small pharmaceutical molecules. Therefore, the development of efficient and convenient methods for synthesizing compounds containing chiral C-N bonds is a crucial area of research. Nicotinamide-dependent oxidoreductases (NDOs) emerge as promising biocatalysts for asymmetric synthesis of chiral C-N bonds due to their mild reaction conditions, exceptional stereoselectivity, high atom economy, and environmentally friendly nature. This review aims to present the structural characteristics and catalytic mechanisms of various NDOs, including imine reductases/ketimine reductases, reductive aminases, EneIRED, and amino acid dehydrogenases. Additionally, the review highlights protein engineering strategies employed to modify the stereoselectivity, substrate specificity, and cofactor preference of NDOs. Furthermore, the applications of NDOs in synthesizing essential medicinal chemicals, such as noncanonical amino acids and chiral amine compounds, are extensively examined. Finally, the review outlines future perspectives by addressing challenges and discussing the potential of utilizing NDOs to establish efficient biosynthesis platforms for C-N bond synthesis. In conclusion, NDOs provide an economical, efficient, and environmentally friendly toolbox for asymmetric synthesis of C-N bonds, thus contributing significantly to the field of pharmaceutical chemical development.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":null,"pages":null},"PeriodicalIF":8.1,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142125086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization of dragon fruit waste to value-added bioproducts and formulations: A review. 火龙果废料的增值生物产品和配方：综述。

IF 8.1 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2024-09-01 Epub Date: 2023-09-24 DOI: 10.1080/07388551.2023.2254930

Manikant Tripathi, Deepti Diwan, Amritesh Chandra Shukla, James Gaffey, Neelam Pathak, Kavya Dashora, Ashok Pandey, Minaxi Sharma, Sanjay Guleria, Sunita Varjani, Quang D Nguyen, Vijai K Gupta

{"title":"Valorization of dragon fruit waste to value-added bioproducts and formulations: A review.","authors":"Manikant Tripathi, Deepti Diwan, Amritesh Chandra Shukla, James Gaffey, Neelam Pathak, Kavya Dashora, Ashok Pandey, Minaxi Sharma, Sanjay Guleria, Sunita Varjani, Quang D Nguyen, Vijai K Gupta","doi":"10.1080/07388551.2023.2254930","DOIUrl":"10.1080/07388551.2023.2254930","url":null,"abstract":"Owing to the increasing worldwide population explosion, managing waste generated from the food sector has become a cross-cutting issue globally, leading to environmental, economic, and social issues. Circular economy-inspired waste valorization approaches have been increasing steadily, generating new business opportunities developing valuable bioproducts using food waste, especially fruit wastes, that may have several applications in energy-food-pharma sectors. Dragon fruit waste is one such waste resource, which is rich in several value-added chemicals and oils, and can be a renewable resource to produce several value-added compounds of potential applications in different industries. Pretreatment and extraction processes in biorefineries are important strategies for recovering value-added biomolecules. There are different methods of valorization, including green extractions and biological conversion approaches. However, microbe-based conversion is one of the advanced technologies for valorizing dragon fruit waste into bioethanol, bioactive products, pharmaceuticals, and other valued products by reusing or recycling them. This state-of-the-art review briefly overviews the dragon fruit waste management strategies and advanced eco-friendly and cost-effective valorization technologies. Furthermore, various applications of different valuable bioactive components obtained from dragon fruit waste have been critically discussed concerning various industrial sectors. Several industrial sectors, such as food, pharmaceuticals, and biofuels, have been critically reviewed in detail.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":null,"pages":null},"PeriodicalIF":8.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41104764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity and regioselectivity of O-methyltransferases catalyzing the formation of O-methylated flavonoids. 催化o -甲基化黄酮形成的o -甲基转移酶的多样性和区域选择性。

IF 8.1 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2024-09-01 Epub Date: 2023-11-30 DOI: 10.1080/07388551.2023.2280755

Juan Wang, Ning Liao, Guanwen Liu, Yinghui Li, Fengqin Xu, Junling Shi

{"title":"Diversity and regioselectivity of O-methyltransferases catalyzing the formation of O-methylated flavonoids.","authors":"Juan Wang, Ning Liao, Guanwen Liu, Yinghui Li, Fengqin Xu, Junling Shi","doi":"10.1080/07388551.2023.2280755","DOIUrl":"10.1080/07388551.2023.2280755","url":null,"abstract":"Flavonoids and their methylated derivatives have immense market potential in the food and biomedical industries due to their multiple beneficial effects, such as antimicrobial, anti-inflammatory, and anticancer activities. The biological synthesis of flavonoids and their derivatives is often accomplished via the use of genetically modified microorganisms to ensure large-scale production. Therefore, it is pivotal to understand the properties of O-methyltransferases (OMTs) that mediate the methylation of flavonoids. However, the properties of these OMTs are governed by their: sources, substrate specificity, amino acid residues in the active sites, and the intricate mechanism. In order to obtain a clue for the selection of suitable OMTs for the biosynthesis of a target methylated flavonoid, we made a comprehensive review of the currently reported results, with a particular focus on their comparative regioselectivity for different flavonoid substrates. Additionally, the possible mechanisms for the diversity of this class of enzymes were explored using molecular simulation technology. Finally, major gaps in our understanding and areas for future studies were discussed. The findings of this study may be useful in selecting genes that encode OMTs and designing enzyme-based processes for synthesizing O-methylated flavonoids.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":null,"pages":null},"PeriodicalIF":8.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138458392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0