Critical Reviews in Biotechnology最新文献

{"title":"A story of two kingdoms: unravelling the intricacies of protein phase separation in plants and animals.","authors":"Min Li, Xue Yang, Di Zhang, Yuan Tian, Zi-Chang Jia, Wen-Hui Liu, Rui-Rui Hao, Yun-Sheng Chen, Mo-Xian Chen, Ying-Gao Liu","doi":"10.1080/07388551.2024.2425989","DOIUrl":"10.1080/07388551.2024.2425989","url":null,"abstract":"<p><p>The biomolecular condensates (BCs) formed by proteins through phase separation provide the necessary space and raw materials for the orderly progression of cellular activities, and on this basis, various membraneless organelles (MLOs) are formed. The occurrence of eukaryotic phase separation is driven by multivalent interactions from intrinsically disordered regions (IDRs) and/or specific protein/nucleic acid binding domains and is regulated by various environmental factors. In plant and animal cells, the MLOs involved in gene expression regulation, stress response, and mitotic control display similar functions and mechanisms. In contrast, the phase separation related to reproductive development and immune regulation differs significantly between the two kingdoms owing to their distinct cell structures and nutritional patterns. In addition, animals and plants each exhibit unique protein phase separation activities, such as neural regulation and light signal response. By comparing the similarities and differences in the formation mechanism and functional regulation of known protein phase separation, we elucidated its importance in the evolution, differentiation, and environmental adaptation of both animals and plants. The significance of studying protein phase separation for enhancing biological quality of life has been further emphasized.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1019-1039"},"PeriodicalIF":8.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142726727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitigating toxic formaldehyde to promote efficient utilization of C1 resources.","authors":"Mengshi Jia, Lei Shao, Jie Jiang, Wankui Jiang, Fengxue Xin, Wenming Zhang, Yujia Jiang, Min Jiang","doi":"10.1080/07388551.2024.2430476","DOIUrl":"10.1080/07388551.2024.2430476","url":null,"abstract":"<p><p>The C1 resource is widely considered because of its abundance and affordability. In the context of extensive utilization of C1 resources by methylotrophic microorganisms, especially for methanol, formaldehyde is an important intermediate metabolite that is at the crossroads of assimilation and dissimilation pathways. However, formaldehyde is an exceedingly reactive compound that can form covalent cross-linked complexes with amine and thiol groups in cells, which causes irreversible damage to the organism. Thus, it is important to balance the intensity of the assimilation and dissimilation pathways of formaldehyde, which can avoid formaldehyde toxicity and improve the full utilization of C1 resources. This review details the source of endogenous formaldehyde and its toxicity mechanism, explaining the harm of excessive accumulation of formaldehyde to metabolism. Importantly, the self-detoxification and various feasible strategies to mitigate formaldehyde toxicity are discussed and proposed. These strategies are meant to help appropriately handle formaldehyde toxicity and accelerate the effective use of C1 resources.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1175-1187"},"PeriodicalIF":8.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNAi-biofungicides: a quantum leap for tree fungal pathogen management.","authors":"Gothandapani Sellamuthu, Amrita Chakraborty, Ramesh R Vetukuri, Saravanasakthi Sarath, Amit Roy","doi":"10.1080/07388551.2024.2430478","DOIUrl":"10.1080/07388551.2024.2430478","url":null,"abstract":"<p><p>Fungal diseases threaten the forest ecosystem, impacting tree health, productivity, and biodiversity. Conventional approaches to combating diseases, such as biological control or fungicides, often reach limits regarding efficacy, resistance, non-target organisms, and environmental impact, enforcing alternative approaches. From an environmental and ecological standpoint, an RNA interference (RNAi) mediated double-stranded RNA (dsRNA)-based strategy can effectively manage forest fungal pathogens. The RNAi approach explicitly targets and suppresses gene expression through a conserved regulatory mechanism. Recently, it has evolved to be an effective tool in combating fungal diseases and promoting sustainable forest management approaches. RNAi bio-fungicides provide efficient and eco-friendly disease control alternatives using species-specific gene targeting, minimizing the off-target effects. With accessible data on fungal disease outbreaks, genomic resources, and effective delivery systems, RNAi-based biofungicides can be a promising tool for managing fungal pathogens in forests. However, concerns regarding the environmental fate of RNAi molecules and their potential impact on non-target organisms require an extensive investigation on a case-to-case basis. The current review critically evaluates the feasibility of RNAi bio-fungicides against forest pathogens by delving into the accessible delivery methods, environmental persistence, regulatory aspects, cost-effectiveness, community acceptance, and plausible future of RNAi-based forest protection products.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1131-1158"},"PeriodicalIF":8.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive peptides with potential anticancer properties from various food protein sources: status of recent research, production technologies, and developments.","authors":"Marwa Hamdi, Bhanu Priya Kilari, Priti Mudgil, Nilesh Prakash Nirmal, Shreesh Ojha, Mohammed Akli Ayoub, Amr Amin, Sajid Maqsood","doi":"10.1080/07388551.2024.2435965","DOIUrl":"10.1080/07388551.2024.2435965","url":null,"abstract":"<p><p>Recently, bioactive peptides, from natural resources, have attracted remarkable attention as nutraceutical treasures and the health benefits of their consumption have extensively been studied. Therapies based on bioactive peptides have been recognized as an innovative and promising alternative method for dangerous diseases such as cancer. Indeed, there has been enormous interest in nutraceuticals and bioactive-based chemopreventive molecules as a potential opportunity to manage chronic diseases, including cancer at different stages, rather than the traditionally used therapies. The relative safety and efficacy of these peptides in targeting only the tumor cells without affecting the normal cells make them attractive alternatives to existing pharmaceuticals for the treatment, management, and prevention of cancer, being able to act as potential physiological modulators of metabolism during their intestinal digestion. Novel bioactive peptides derived from food sources can be beneficial as anticancer nutraceuticals and provide a basis for the pharmaceutical development of food-derived bioactive peptides. Bioactive peptides can be generated through different protein hydrolysis methods and purified using advanced chromatographic techniques. Moreover, establishing bioactive peptides' efficacy and mechanism of action can provide alternative methods for cancer prevention and management. Most of the research on anticancer peptides is carried out on cell lines with very limited research being investigated in animal models or human clinical models. In this context, this review article comprehensively discusses anticancer peptides': production, isolation, therapeutic strategies, mechanism of action, and application in cancer therapy.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1076-1097"},"PeriodicalIF":8.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current status and advances in the green synthesis of muconic acid.","authors":"Haoyi Yang, Xiaoyu Lin, Xianen Zhong, Mingfeng Cao, Jifeng Yuan, Zhipeng Li, Xueping Ling, Ning He","doi":"10.1080/07388551.2024.2433998","DOIUrl":"10.1080/07388551.2024.2433998","url":null,"abstract":"<p><p>Muconic acid (MA) is a valuable dicarboxylic acid with three isomers that are extensively utilized in textile and chemical industries. Traditionally, the chemical synthesis of MA consumes nonrenewable petrochemical raw materials and causes significant environmental problems. With the rapid increase in demand for MA, eco-friendly biosynthetic technologies with renewable sources are becoming ideal alternative solutions. This paper systematically reviews recent advances in the biosynthesis of MA isomers, describing not only the mechanism for MA biosynthesis in different microorganisms, including wild and engineered strains, but also focuses on MA production from various renewable resources, especially lignin hydrolysate and lignin-derived aromatics hydrocarbons, such as: benzoic acid, isoeugenol, vanillic acid and phenol. Moreover, <i>cis,cis</i>-muconic acid production from xylose, PET, methane, and glycerol are discussed in detail, providing a much broader substrate spectra and further possibilities for MA large scale industrialization economically. Challenges facing biosynthesis of cis, trans muconic acid and trans, trans muconic acid are discussed finally.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1040-1057"},"PeriodicalIF":8.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xylooligosaccharides mediated gut microbiome modulation: prebiotics to postbiotics.","authors":"Vishal Kumar, Ashutosh Bahuguna, Subhash Kumar, Myunghee Kim","doi":"10.1080/07388551.2025.2460852","DOIUrl":"10.1080/07388551.2025.2460852","url":null,"abstract":"<p><p>An increasing trend toward harnessing nutraceuticals as food supplements rather than pharmaceuticals as curative and preventive agents against various ailments has been observed. Owing to their health benefits, prebiotics have received notable attention from the pharmaceutical and food industries. Among the different prebiotic oligosaccharides, xylooligosaccharides (XOS) exhibited a remarkable capacity to stimulate the growth of the gut microbiota and benefit individuals with metabolic abnormalities. Additionally, XOS can be produced from various renewable agricultural wastes, which supports their economic feasibility for use as prebiotics at the industrial level. This review explains gut microbiome modulation based on <i>in vivo</i>, <i>in vitro</i>, and clinical findings. Gut microbiome modulation leads to the production of postbiotics that stimulate various beneficial health effects. The current review entails the mechanisms of different health-promoting activities mediated by XOS, including immunomodulation and anticancer effects. Additionally, the concept of converting prebiotics to synbiotics using XOS has been established for nutraceutical applications. Synbiotics based on XOS and probiotics may be good alternatives to nutraceuticals for improving human health.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1098-1116"},"PeriodicalIF":8.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knot-knot chronicles: unveiling the G-quadruplexes.","authors":"Himanshi Sharma, Rohini Garg","doi":"10.1080/07388551.2024.2435960","DOIUrl":"https://doi.org/10.1080/07388551.2024.2435960","url":null,"abstract":"<p><p>G-quadruplex structures (GQSes) are the intricate molecular knots or marvels that play diverse roles in various cellular processes, such as replication, transcription, and translation, which regulate gene expression. Even though GQSes can be found throughout the genome, they are more prevalent in certain genomic regions like promoters and 5'-UTRs. This review discusses the functionality of GQSes across various regions of the genome and draws attention to the intriguing world of DNA and RNA GQSes. We highlight the uniqueness and similarities of GQSes in DNA and RNA. The functional roles of various proteins that interact with GQSes are also discussed. In addition, the role of GQSes in the context of plant development has been elaborated. The approaches for detecting GQSes using different methods and their influence in regulating gene regulation are also described. We provide insights into how GQSes can be used as potential game changers in plant biotechnology by utilizing them as regulatory switches to control various aspects of transcriptional and post-transcriptional processes depending on the arrangement of GQSes.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":"45 5","pages":"1159-1174"},"PeriodicalIF":8.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144583318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mechanisms of environmental stress tolerance in <i>Gluconobacter oxydans</i>: progress and perspectives.","authors":"Yan Chen, Fei Liu, Aobo Sha, Meijuan Xu, Zhiming Rao, Xian Zhang","doi":"10.1080/07388551.2024.2426011","DOIUrl":"10.1080/07388551.2024.2426011","url":null,"abstract":"<p><p><i>Gluconobacter oxydans</i> have been widely used in industrial compound production for their incomplete oxidation ability. However, they are often subjected to a wide variety of severe environmental stresses, such as extreme pH, high temperature, osmotic pressure, and organic solvents, which greatly repress microbial growth viability and productivity. As typical biocatalysis chassis cells with a high tolerance to external environmental stresses, it is extremely important to construct highly tolerant chassis cells and understand the tolerance mechanisms of <i>G. oxydans</i> and how different stresses interact with the cell: membranes, phospholipid bilayers, transporters, and chaperone proteins. In this review, we discuss and summarize the mechanisms of environmental stress tolerance in <i>G. oxydans</i>, and the promising strategies that can be used to further construct tolerant strains are prospected.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1117-1130"},"PeriodicalIF":8.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological production of nicotinamide mononucleotide: a review.","authors":"Rhudith B Cabulong, Saroj Raj Kafle, Anju Singh, Mukesh Sharma, Beom Soo Kim","doi":"10.1080/07388551.2024.2433993","DOIUrl":"10.1080/07388551.2024.2433993","url":null,"abstract":"<p><p>Nicotinamide mononucleotide (NMN) presents significant therapeutic potential against aging-related conditions, such as Alzheimer's disease, due to its consistent and strong pharmacological effects. Aside from its anti-aging effect, NMN is also an emerging noncanonical cofactor for orthogonal metabolic pathways in the field of biomanufacturing. This has significant advantages in the field of metabolic engineering, allowing cells to produce unnatural chemicals without disrupting the natural cellular processes. NMN is produced through both the chemical and biological methods, with the latter being more environmentally sustainable. The primary biological production pathway centers on the enzyme nicotinamide phosphoribosyltransferase, which transforms nicotinamide and phosphoribosyl pyrophosphate to NMN. Efforts to increase NMN production have been explored in microorganisms, such as: <i>Escherichia coli, Bacillus subtilis,</i> and yeast, serving as biocatalysts, by rewiring their metabolic processes. Although most researchers are focusing on genetically and metabolically manipulating microorganisms to act as biocatalysts, a growing number of studies on cell-free synthesis are emerging as a promising strategy for producing NMN. This review explores the different biological production techniques of NMN employing microorganisms. This article, in particular, is essential to those who are working on NMN production using microbial strain engineering and cell-free systems.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1058-1075"},"PeriodicalIF":8.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in sustainable strategies for development of innovative nanobiocatalysts using immobilized β-glucosidase for industrial applications.","authors":"Shivangi Chamoli, Shimali, Ambika Chamoli, Kachan Karki, Ravendra Kumar, Vinod Kumar, Piyush Kumar","doi":"10.1080/07388551.2025.2517714","DOIUrl":"https://doi.org/10.1080/07388551.2025.2517714","url":null,"abstract":"<p><p>β-glucosidases are a well-characterized, diverse group of hydrolytic enzymes that act on various substrates. They are extensively used in different sectors, including: bioethanol, food, flavor, nutraceutical, and pharmaceutical industries. Immobilization improves the operational stability, reusability and catalytic efficiency of β-glucosidase compared to the free enzyme. The nanoscale dimensions, high surface area of the nanomaterial, and strong enzyme-nanosupport interactions prevent denaturation and leaching of β-glucosidase. This boosts enzyme stability, reduces the need for replenishment, and allows for easy recovery and reuse, minimizing enzyme waste and energy consumption in industrial biocatalysis. Nanosupport materials, including: inorganic materials, carbon, biopolymer-based, and magnetic nanoparticles, have gained popularity as immobilization matrices for generating either β-glucosidase immobilization or co-immobilization systems for various applications. The present review focuses on the current trends in immobilization strategies of β-glucosidase for improving operational stability and recyclability of the enzyme. Additionally, this review provides deeper insights into various surface modifications of magnetic and non-magnetic nanosupport matrices employed for immobilization and their impact on the catalytic efficiency of β-glucosidase. Moreover, the review thoroughly investigates the challenges encountered in immobilizing β-glucosidases on various nanosupport matrices. It concludes with insightful remarks that encourage future researchers to conduct studies dedicated to the development of a highly efficient, industrially adapted nanobiocatalytic system to achieve sustainable biotransformation aligning with United Nations Sustainable Development Goals (SDG): SDG 2 (Sustainable Food System), SDG 7 (Affordable and Clean Energy), SDG 9 (Sustainable Industry), SDG 12 (Responsible Consumption), and SDG 13 (Climate Action: Reducing Carbon Emissions).</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1-20"},"PeriodicalIF":8.1,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}