Critical Reviews in Biotechnology最新文献

{"title":"Transcriptional control in microalgae: co-regulated fatty acid biosynthesis and carbon dioxide fixation.","authors":"Sadaf-Ilyas Kayani, Xinjuan Hu, Qian Shen, Bin Zou, Feifei Zhu, Zhen Yu, Muhammad Abdur Rehman Shah, Obaid Ur Rehman, Shuhao Huo","doi":"10.1080/07388551.2025.2503788","DOIUrl":"https://doi.org/10.1080/07388551.2025.2503788","url":null,"abstract":"<p><p>Microalgae are desirable candidates for performing about half of the World's organic carbon fixation and its conversion to essential metabolites of human metabolism, including polyunsaturated fatty acids (PUFAs). However, the yields of microalgal FAs produced naturally are typically insufficient to cover the expenses of their commercial utilization. To overcome this problem, gene engineering techniques have been used to change the activity of endogenous enzymes. This review aims to find knowledge about the mechanism of regulation of fatty acid (FA) biosynthesis and CO₂ fixation in microalgae. Firstly, this study discusses molecular strategies toward accelerating FA biosynthesis with a main emphasis on a critical review of transcriptional engineering. Some transcription factors (TFs) are known to increase FA content and related gene expression. However, a research gap is revealed toward understanding their regulatory mechanism and finding their role in regulating CO₂ fixation. Secondly, a critical review of studies on CO₂ fixation regulated by Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) and RuBisCo activase (<i>RCA</i>) disclosed that no studies have yet been reported about their transcriptional control. Thirdly, prospects are given on the genetic basis of parallel transcriptional regulation of genes involved in FA biosynthesis and CO₂ fixation in microalgae. This study should potentially provide considerable knowledge on developing eco-friendly and sustainable microalgae genetic resources to maximize the yield of value-added FAs using TF engineering.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1-22"},"PeriodicalIF":8.1,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing <i>Bacillus</i> keratinases for sustainable keratin waste valorization: a current appraisal.","authors":"Pranshi Gupta, Isha Sharma, Naveen Kango","doi":"10.1080/07388551.2025.2495281","DOIUrl":"https://doi.org/10.1080/07388551.2025.2495281","url":null,"abstract":"<p><p>The global accumulation of keratin-rich waste, primarily from poultry and livestock industries, presents significant environmental and economic challenges. This review explores the potential of <i>Bacillus</i>-derived keratinases as a sustainable solution for keratin waste valorization and prospects of value-addition. Keratinases, the keratin hydrolyzing proteases produced predominantly by various <i>Bacillus</i> species, exhibit exceptional capability in degrading keratin, a highly stable and recalcitrant protein. This degradation process not only mitigates the environmental impact of keratin waste, but also converts it into valuable by-products with potential industrial applications. We systematically review various aspects, including: the production, properties and the mechanism of keratin degradation by <i>Bacillus</i> keratinases, highlighting their enzymatic properties, substrate specificity, and efficiency in valorizing keratin into peptides and amino acids. Biomolecular aspects and catalytic behavior relevant to the activity and stability of <i>Bacillus</i> keratinases are visited <i>via in silico</i> modeling. The economic and environmental benefits of utilizing keratinases for waste valorization are assessed, including reductions in waste disposal costs, greenhouse gas emissions, and the potential for creating new economic opportunities through the utilization of keratin-derived products. The recent advancements in keratin waste enzyme treatment and their utilization in developing circular bioeconomy are highlighted in the present article.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1-24"},"PeriodicalIF":8.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calcium-dependent protein kinase (CDPK/CPK)-mediated salicylic acid cascade: the key arsenal of plants under pathogens attack.","authors":"Farhan Goher, Faiza Shafique Khan, Shengren Sun, Qinnan Wang","doi":"10.1080/07388551.2025.2498463","DOIUrl":"https://doi.org/10.1080/07388551.2025.2498463","url":null,"abstract":"<p><p>Upon pathogen attack, cytosolic Ca²⁺ levels increase in plant cells. The first innate immune response is activated by detecting microbe/pathogen-associated molecular patterns (MAMPs/PAMPs) and is called PAMPs-triggered immunity (PTI). The second immune response is triggered by recognizing pathogens' effector proteins named effectors-triggered immunity (ETI). Calcium-dependent protein kinases (CDPKs or CPKs) are well-known calcium sensors that have a mediator role both in PTI and ETI. Calcium can bind to the elongation factor (EF)-hand domain at the C-terminus of CDPKs, which then phosphorylates substrates at the N-terminal catalytic kinase domain to transfer calcium signals directly. Improving the stress resilience of crops is a critical strategy in attaining global food security. In plants, when a stimulus is seen, there is an increase in Ca²⁺ concentration, which activates CDPKs which are in charge of sending out the immunological signals needed for disease tolerance. During the immune response, CDPKs are subject to numerous levels of regulation, including Ca²⁺ dependency to decipher various Ca²⁺ signals. Furthermore, salicylic acid (SA) regulation by CDPKs provides a comprehensive overview of CDPKs-mediated SA signaling during immune response in plants under pathogen attack. The critical part of CDPKs in SA biosynthesis, from the regulation of SA biosynthesis to how NPR1 perceives SA upon biotic stress, is comprehensively reviewed in this paper with the latest advancements in research. However, more research about CDPKs-mediated SA signaling under pathogen attack is mandatory to further dissect their co-role in crop protection against various diseases to achieve sustainable production goals in the future.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1-17"},"PeriodicalIF":8.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Upcoming generation nanoengineered antimicrobial delivery system for targeting multidrug-resistant microbes.","authors":"Aditya Upadhyay, Dharm Pal, Awanish Kumar","doi":"10.1080/07388551.2025.2506611","DOIUrl":"https://doi.org/10.1080/07388551.2025.2506611","url":null,"abstract":"<p><p>The rise of chronic and acute infections has increased reliance on antimicrobial agents. However, conventional antimicrobials often fail to deliver optimal therapeutic outcomes due to limitations such as low bioavailability, poor biocompatibility, nonspecific targeting, drug-induced toxicity, and the growing issue of antimicrobial resistance. Therefore, the concept of a resistance-proof antimicrobial agent (RPAA) and its smart delivery was introduced to overcome the existing problem and a targeted delivery due to the specific properties, such as: high bioavailability, biocompatibility, low drug-induced toxicity, biodegradability, high binding capacity with the pathogen, multiple targeting delivery, etc. This system generates a positive impact and could quash the multidrug resistance problem. In this review, we discuss: the rationale for developing a nanoengineering-based smart-delivery system for RPAA, the advantageous properties of such a system, the possible mechanism of delivery, and challenges in the development of a nano-drug delivery therapeutics tool for RPAA delivery as a solution to combat the global problem of drug resistance. We emphasize the urgent need for the development of such a next-generation drug delivery system and discuss the opportunities/hurdles as well as the questions that remain to be addressed. The article is important because it sheds light on the properties of nanoengineered drug delivery that could initiate new ways of thinking about the development of future-generation delivery systems. The article shares a promising idea that would be an essential foundation for opening a new window in the field of drug discovery and development of the smart delivery system for RPAA.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1-20"},"PeriodicalIF":8.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amplification-free nucleic acids detection with next-generation CRISPR/dx systems.","authors":"Cia-Hin Lau, Siping Huang, Haibao Zhu","doi":"10.1080/07388551.2024.2399560","DOIUrl":"10.1080/07388551.2024.2399560","url":null,"abstract":"<p><p>CRISPR-based diagnostics (CRISPR/Dx) have revolutionized the field of molecular diagnostics. It enables home self-test, field-deployable, and point-of-care testing (POCT). Despite the great potential of CRISPR/Dx in diagnoses of biologically complex diseases, preamplification of the template often is required for the sensitive detection of low-abundance nucleic acids. Various amplification-free CRISPR/Dx systems were recently developed to enhance signal detection at sufficient sensitivity. Broadly, these amplification-free CRISPR/Dx systems are classified into five groups depending on the signal enhancement strategies employed: CRISPR/Cas12a and/or CRISPR/Cas13a are integrated with: (1) other catalytic enzymes (Cas14a, Csm6, Argonaute, duplex-specific nuclease, nanozyme, or T7 exonuclease), (2) rational-designed oligonucleotides (multivalent aptamer, tetrahedral DNA framework, RNA G-quadruplexes, DNA roller machine, switchable-caged guide RNA, hybrid locked RNA/DNA probe, hybridized cascade probe, or \"U\" rich stem-loop RNA), (3) nanomaterials (nanophotonic structure, gold nanoparticle, micromotor, or microbeads), (4) electrochemical and piezoelectric plate biosensors (SERS nanoprobes, graphene field-effect transistor, redox probe, or primer exchange reaction), or (5) cutting-edge detection technology platforms (digital bioanalysis, droplet microfluidic, smartphone camera, or single nanoparticle counting). Herein, we critically discuss the advances, pitfalls and future perspectives for these amplification-free CRISPR/Dx systems in nucleic acids detection. The continued refinement of these CRISPR/Dx systems will pave the road for rapid, cost-effective, ultrasensitive, and ultraspecific on-site detection without resorting to target amplification, with the ultimate goal of establishing CRISPR/Dx as the paragon of diagnostics.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"859-886"},"PeriodicalIF":8.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142281514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pilot scale polyhydroxyalkanoates biopolymer production using pure cultures: current status and future opportunities.","authors":"Phavit Wongsirichot","doi":"10.1080/07388551.2024.2409112","DOIUrl":"10.1080/07388551.2024.2409112","url":null,"abstract":"<p><p>The development and commercialization of bio-based and biodegradable polyhydroxyalkanoates (PHAs) biopolymers could be crucial for the transition toward a sustainable circular economy. However, despite potential traditional and novel applications in the packaging, textiles, agriculture, automotive, electronics, and biomedical industries, the commercialization of PHAs is limited by their current market competitiveness. This review provides the first critical assessment of the current pure culture pilot-scale PHA literature, which could be crucial in translating promising laboratory-scale developments into industrial-scale commercial PHA production. It will also complement reviews of mixed microbial cultures currently dominating pilot-scale PHA literature. Pure culture fermentations could provide advantages, such as ease of characterizing microbial producers' behavior, higher PHA productivities, and better alignment with existing PHA commercialization and industrial biotechnology approaches. Key aspects, including producer organisms, fermentation volumes and schemes, control schemes, optimization, and properties of the polymers produced, are discussed in-depth, to elucidate important trends, achievements, and knowledge gaps. Furthermore, specific ways for future pilot-scale studies to help address current PHA commercialization challenges are also identified. The insights, and recommendations provided will be extremely beneficial for the future development of PHA production, at both pilot and commercial scales, whilst also being beneficial to the production of other microbial polymers and industrial biotechnology as a whole.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"887-903"},"PeriodicalIF":8.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Farm to fork applications: how vibrational spectroscopy can be used along the whole value chain?","authors":"Ravi Pandiselvam, Alev Yüksel Aydar, Zeynep Aksoylu Özbek, Didem Sözeri Atik, Özge Süfer, Bilge Taşkin, Emine Olum, Seema Ramniwas, Sarvesh Rustagi, Daniel Cozzolino","doi":"10.1080/07388551.2024.2409124","DOIUrl":"10.1080/07388551.2024.2409124","url":null,"abstract":"<p><p>Vibrational spectroscopy is a nondestructive analysis technique that depends on the periodic variations in dipole moments and polarizabilities resulting from the molecular vibrations of molecules/atoms. These methods have important advantages over conventional analytical techniques, including (a) their simplicity in terms of implementation and operation, (b) their adaptability to on-line and on-farm applications, (c) making measurement in a few minutes, and (d) the absence of dangerous solvents throughout sample preparation or measurement. Food safety is a concept that requires the assurance that food is free from any physical, chemical, or biological hazards at all stages, from farm to fork. Continuous monitoring should be provided in order to guarantee the safety of the food. Regarding their advantages, vibrational spectroscopic methods, such as Fourier-transform infrared (FTIR), near-infrared (NIR), and Raman spectroscopy, are considered reliable and rapid techniques to track food safety- and food authenticity-related issues throughout the food chain. Furthermore, coupling spectral data with chemometric approaches also enables the discrimination of samples with different kinds of food safety-related hazards. This review deals with the recent application of vibrational spectroscopic techniques to monitor various hazards related to various foods, including crops, fruits, vegetables, milk, dairy products, meat, seafood, and poultry, throughout harvesting, transportation, processing, distribution, and storage.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"938-981"},"PeriodicalIF":8.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Critical analysis of analytical techniques developed for statins in biological fluids, environmental and fermentation samples.","authors":"Seenivasan Ayothiraman, Nithya Murugesan, Gautam Sethi","doi":"10.1080/07388551.2024.2412128","DOIUrl":"10.1080/07388551.2024.2412128","url":null,"abstract":"<p><p>Statins are the most prescribed drug for regulating the high cholesterol level in the blood, which can lead to severe complications, such as cardiovascular diseases and other health complications. A wide range of analytical techniques have been employed for the quantification of statins from various origins, including fermentation derived (lovastatin, pravastatin, and compactin), semi-synthetic (simvastatin), and synthetic (atorvastatin, rosuvastatin, and fluvastatin) routes. The presence of more than one structural form and structural analogue generated in the biosynthesis pathway, as well as reaction intermediates and macromolecules in the clinical sample, complicates the quantification of statins. Furthermore, significant concentrations of statins in environmental samples pose serious health and ecology hazards, and estimating statins in those diluted samples is extremely difficult. On the other hand, the: cost, accurate estimation of the desired one from other structural forms, sample complexity, time, limits of detection and quantification, were major criteria distinguishing the usability of each technique. As a result, the current manuscript focuses on analytical techniques such as molecular spectroscopy (normal and derivatives UV-Visible spectrophotometer), chromatography (TLC, HP-TLC, HPLC, GC, swing column, micellar, and supercritical fluid), mass spectroscopy (HPLC-MS/MS and GC-MS/MS), sequential flow injection, capillary electrophoresis, and cyclic voltammetry, as well as their: optimal operating conditions, limits of detection and quantification, advancements, and limitations. Furthermore, various online and offline sample preparations (precipitation, solid phase extraction, liquid-liquid extraction, and micellar extraction) have been highlighted as an essential pretreatment technique to avoid the interference caused by structural analogues and other macromolecules. The greener and more sustainable concepts used in analytical approaches for the quantification statins are also highlighted with current advancements.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"805-835"},"PeriodicalIF":8.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing cyclotide bioproduction: harnessing biological synthesis methods and various expression systems for large-scale manufacturing.","authors":"Mohammad Sadegh Taghizadeh, Ali Niazi, Armin Mirzapour-Kouhdasht, Eric C Pereira, Marco Garcia-Vaquero","doi":"10.1080/07388551.2024.2412780","DOIUrl":"10.1080/07388551.2024.2412780","url":null,"abstract":"<p><p>Peptide-based medications hold immense potential in addressing a wide range of human disorders and discomforts. However, their widespread utilization encounters two major challenges: preservation and production efficiency. Cyclotides, a class of ribosomally synthesized and post-translationally modified peptides (RiPPs), exhibit unique characteristics, such as a cyclic backbone and cystine knot, enhancing their stability and contributing to a wide range of pharmacological properties exhibited by these compounds. Cyclotides are efficient in the biomedical (e.g., antitumor, antidiabetic, antimicrobial, antiviral) and agrochemical fields by exhibiting activity against pests and plant diseases. Furthermore, their structural attributes make them suitable as molecular scaffolds for grafting and drug delivery. Notably, the mutated variant of kalata B1 cyclotide ([T20K] kalata B1) has recently entered phase 1 of human clinical trials for multiple sclerosis, building upon the success observed in animal trials. To enable large-scale production of cyclotides, it is crucial to further explore their remarkable structural and bioactive properties. This necessitates extensive research focused on enhancing the efficiency of the processes required for their production. This study provides a comprehensive review of the biological synthesis methods of cyclotides, with particular emphasis on various expression systems, namely bacteria, plants, yeast, and cell-free systems. By investigating these expression systems, it becomes possible to design production systems that are adaptable, economically viable, and efficient for generating active and pure cyclotides at an industrial scale. The advantages of biological synthesis over chemical synthesis are thoroughly explored, highlighting the potential of these expression systems in meeting the demands of large-scale cyclotide production.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"836-858"},"PeriodicalIF":8.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Value-added biotransformation of agricultural byproducts by cellulolytic fungi: a review.","authors":"Su Yan, Jia-Jia Ma, Dan Wu, Gui-Li Huang, Xiao-Wei Yu, Yu-Ning Wang","doi":"10.1080/07388551.2024.2423152","DOIUrl":"10.1080/07388551.2024.2423152","url":null,"abstract":"<p><p>Agricultural byproducts generally contain abundant bioactive compounds (e.g., cellulose/hemicellulose, phenolic compounds (PCs), and dietary fibers (DFs)), but most of them are neglected and underutilized. Owing to the complicated and rigid structures of agricultural byproducts, a considerable amount of bioactive compounds are entrapped in the polymer matrix, impeding their further development and utilization. In recent years, the prominent performance of cellulolytic fungi to grow and degrade agricultural byproducts has been applied to achieve efficient biotransformation of byproducts to high-value compounds, which is a green and sustainable strategy for the reutilization of agricultural byproducts. This review comprehensively summarizes recent progress in the value-added biotransformation of agricultural byproducts by cellulolytic fungi, including (1) direct utilization of agricultural byproducts for biochemicals and bioethanol production via a consolidated bioprocessing, (2) recovery and biotransformation of bounded PCs from agricultural byproducts for higher bioactive properties, as well as (3) modification and conversion of insoluble DF from agricultural byproducts to produce functional soluble DF. The functional enzymes, potential mechanisms, and metabolic pathways involved are emphasized. Moreover, promising advantages and current bottlenecks using cellulolytic fungi have also been elucidated, shedding further perspectives for sustainable and efficient reutilization of agricultural byproducts by cellulolytic fungi.</p>","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"982-1001"},"PeriodicalIF":8.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}