Critical Reviews in Biotechnology最新文献

Microbial lipids for a sustainable future: the growing potential of synthetic microbial engineering for consumer oils. 可持续未来的微生物脂质：合成微生物工程用于食用油的日益增长的潜力。

IF 7.7 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2026-05-01 Epub Date: 2026-02-15 DOI: 10.1080/07388551.2026.2615047

Thomas R V Collier, Patrick A da Roza, Hugh D Goold, Surinder Singh, Ian T Paulsen, Roy S K Walker, Isak S Pretorius

引用次数: 0

Advances in primary cell isolation, sorting, and preservation from livestock for cultured meat applications. 畜禽培养肉原代细胞分离、分选和保存的研究进展。

IF 7.7 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2026-05-01 Epub Date: 2026-02-17 DOI: 10.1080/07388551.2026.2618192

Sanghun Park, Gyutae Park, Sehyuk Oh, Sol-Hee Lee, Jungseok Choi

{"title":"Advances in primary cell isolation, sorting, and preservation from livestock for cultured meat applications.","authors":"Sanghun Park, Gyutae Park, Sehyuk Oh, Sol-Hee Lee, Jungseok Choi","doi":"10.1080/07388551.2026.2618192","DOIUrl":"10.1080/07388551.2026.2618192","url":null,"abstract":"Cultured meat production relies on the efficient: isolation, purification, expansion, and preservation of livestock-derived primary cells with robust proliferation and differentiation potential. Satellite cells and fibro-adipogenic progenitors from muscle and adipose tissues are key cellular sources for the in vitro reconstruction of meat-like structures. This review critically examines current methodologies used for primary cell processing in cultured meat scalable bioprocessing research. Tissue dissociation strategies are classified as enzymatic, non-enzymatic, and hybrid protocols and compared in terms of yield, scalability, and preservation of cell functionality. Cell purification techniques include: density gradient centrifugation, pre-plating, fluorescence-activated cell sorting (FACS), and magnetic-activated cell sorting (MACS), each offering different levels of specificity, throughput, and compatibility with food-grade applications. Long-term storage via cryopreservation is essential for establishing reliable cell banks. However, challenges remain in minimizing cryoinjury, selecting optimal cryoprotective agents, and maintaining post-thaw viability. Emerging alternatives, such as temperature-responsive cell sheet technology and biochemical protection strategies, are highlighted for their potential to improve cell recovery and functionality. Unlike existing reviews that often focus on isolated aspects, this article integrates dissociation, purification, and preservation into one framework. It highlights how optimized protocols can overcome current bottlenecks in cell processing. This review emphasizes the importance of standardized and food-safe platforms. Such advances will support commercialization and sustainable delivery of cultured meat products.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"522-540"},"PeriodicalIF":7.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146212368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revisiting enzyme engineering strategies and reaction mechanisms of lytic polysaccharide monooxygenases (LPMOs). 回顾多糖单加氧酶（LPMOs）的酶工程策略和反应机理。

IF 7.7 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2026-05-01 Epub Date: 2026-02-02 DOI: 10.1080/07388551.2025.2612611

Musaddique Hossain, Shalini Das, Subba Reddy Dodda, Kamalpreet Singh, Tiyasa Bhuniya, Tiasha Ghosh, Sudit S Mukhopadhyay

{"title":"Revisiting enzyme engineering strategies and reaction mechanisms of lytic polysaccharide monooxygenases (LPMOs).","authors":"Musaddique Hossain, Shalini Das, Subba Reddy Dodda, Kamalpreet Singh, Tiyasa Bhuniya, Tiasha Ghosh, Sudit S Mukhopadhyay","doi":"10.1080/07388551.2025.2612611","DOIUrl":"10.1080/07388551.2025.2612611","url":null,"abstract":"Lytic polysaccharide monooxygenases (LPMOs) are auxiliary metalloenzymes that play a crucial role in the degradation of polysaccharides through an oxidative mechanism, distinguishing them from the traditional glycoside hydrolases. Although LPMOs were first identified in 1992, their functional identity and unique oxidative activity were not fully understood until the year 2010. These enzymes cleave at the C1 or C4 position of glycosidic bonds in polysaccharides using molecular oxygen and reductants such as ascorbic acid or cellobiose dehydrogenase (CDH). LPMOs exhibit significant sequence diversity across eight known families and operate via complex mechanisms. Structurally, LPMOs have a conserved active site with a copper ion coordinated by two histidine residues, known as the \"histidine brace\" which is crucial for their oxidative activity Their ability to enhance the efficiency of cellulase enzymes makes them highly valuable in the bio refinery industry. This review focuses on details of: regioselectivity, reaction mechanism, protein engineering strategies, and industrial applications of LPMO. It also emphasizes the building correlation between challenges at the industrial level and their possible solutions through enzyme engineering.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"460-476"},"PeriodicalIF":7.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146104369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scope for vitamin B deficiency redressal through microbial vitamins with reference to India and South Africa. 通过微生物维生素治疗维生素B缺乏症的范围参照印度和南非。

IF 7.7 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2026-05-01 Epub Date: 2026-02-19 DOI: 10.1080/07388551.2026.2616412

Priyadarshini Pratikshya Nayak, Tinashe Andrew Gona, Sinesipho Galada, Ngwekazi Nwabisa Mehlomakulu, Gargi Dey, Elna Maria Buys, Sandeep Kumar Panda

{"title":"Scope for vitamin B deficiency redressal through microbial vitamins with reference to India and South Africa.","authors":"Priyadarshini Pratikshya Nayak, Tinashe Andrew Gona, Sinesipho Galada, Ngwekazi Nwabisa Mehlomakulu, Gargi Dey, Elna Maria Buys, Sandeep Kumar Panda","doi":"10.1080/07388551.2026.2616412","DOIUrl":"10.1080/07388551.2026.2616412","url":null,"abstract":"B vitamins are the most widely used supplements for women and children to maintain good health conditions. Vitamin B deficiency is prevalent in many countries including India and South Africa. Synthetic vitamins (such as folic acid) are administered orally to vulnerable groups to address the vitamin B deficiency. B vitamin-fortified foods have also been adopted as the mandate of the governments of India and South Africa. However, the policies have not been able to bring any sustainable solutions to vitamin B deficiency. This article describes the natural production of B vitamins by cultured microorganisms. Furthermore, this article describes the scope of microbial B vitamin availability in India and South Africa through dietary interventions (foods obtained from microbial processing/fermented food products). The article also elucidates the different fermented foods of India and South Africa and the increment of different B vitamins, namely riboflavin (vitamin B2), folate (vitamin B9), and cyanocobalamin (vitamin B12) during the fermentation. The technoeconomical feasibility and commercial aspects have been discussed in the article.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"380-398"},"PeriodicalIF":7.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146225764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brassinosteroid-modified crops: key players for a modern Green Revolution. 油菜素内酯改造作物：现代绿色革命的关键人物。

IF 7.7 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2026-05-01 Epub Date: 2026-03-22 DOI: 10.1080/07388551.2025.2610339

Sunny Ahmar, Marcin Rapacz, Fahad Masoud Wattoo, Ewa Pociecha

{"title":"Brassinosteroid-modified crops: key players for a modern Green Revolution.","authors":"Sunny Ahmar, Marcin Rapacz, Fahad Masoud Wattoo, Ewa Pociecha","doi":"10.1080/07388551.2025.2610339","DOIUrl":"10.1080/07388551.2025.2610339","url":null,"abstract":"With the global population projected to reach 9.8 billion by 2050, coupled with extreme weather events and resource scarcity, there is an urgent need to redesign existing Green Revolution varieties (GRVs) for a modern revolution in agriculture. Brassinosteroids (BRs) play crucial roles in shaping plant architecture and hold significant potential for developing modern GRVs that are high-yielding, climate-resilient and environmentally sustainable. Recent advances in crop improvement, particularly by manipulating BR signaling and biosynthesis genes, have already generated semi-dwarf GRVs. These BR-modified GRVs exhibited enhanced overall crop performance, including yield, photosynthetic capacity and nitrogen-use efficiency. However, they still face challenges regarding tolerance to various biotic and abiotic stresses, although studies on BR mutants have indicated that BRs can confer resilience to biotic and abiotic stresses. Despite such studies having made significant progress in exploring the role of BRs, their functions in crop species are not comprehensively understood. Therefore, additional BR mutants need to be developed to make existing crops climate-resilient and to tailor them to specific regional needs, such as increased frost tolerance in Eastern Europe. Ongoing efforts are focused on identifying candidate BR-related genes in crops to reveal the regulatory mechanisms underlying BR signaling and biosynthesis, thereby improving overall performance. This review highlights recent advances in BR research related to crop improvement and stress adaptation. Moreover, it emphasizes the potential of BRs to drive a new generation of BR-modified GRVs and outlines strategies to achieve this by manipulating BR signaling and biosynthesis pathways to develop high-yield, climate-resilient crops.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"399-418"},"PeriodicalIF":7.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N-Glycosylation differences between recombinant and wildtype glycoproteins revealed by mass spectrometry. 质谱分析显示重组型和野生型糖蛋白的n -糖基化差异。

IF 7.7 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2026-05-01 Epub Date: 2026-03-22 DOI: 10.1080/07388551.2025.2584692

Yang Zou, Dario Cramer, Albert J R Heck, Karli R Reiding

{"title":"N-Glycosylation differences between recombinant and wildtype glycoproteins revealed by mass spectrometry.","authors":"Yang Zou, Dario Cramer, Albert J R Heck, Karli R Reiding","doi":"10.1080/07388551.2025.2584692","DOIUrl":"10.1080/07388551.2025.2584692","url":null,"abstract":"Mass spectrometry (MS) has emerged as a powerful technique to study protein glycosylation. MS on intact denatured or native proteins can reveal all-inclusive glycoproteoform profiles while top-down, middle-down and/or bottom-up MS can uncover the characteristics of individual glycosylation sites. Alternatively, analyzing enzymatically released N-glycans can reveal intricate details on glycan isomers and generate high-throughput data on larger cohorts. All these methods are increasingly applied for the study of both individual glycoproteins and complex glycoprotein mixtures such as those originating from blood plasma or cell lysates. This has increased our knowledge about the complexity of protein glycosylation, but also revealed its huge diversity, which depends not only on the protein but also on the cell-dependent glycosylation machinery that may change with physiological conditions. Currently, multiple glycoproteins are recombinantly produced, for therapeutic applications as well as in the food sector, in host cells of diverse origin, most commonly: E. coli bacteria, yeast cells, insect cells, mammalian CHO or human HEK293 cells. Although glycoproteins of interest might show similar yields when produced in different host cells, an important question remains whether the host cell will or can provide similar or alike glycoproteoform profiles. In this review, we focus on the application of MS-based technologies to study glycosylation profiles of endogenous human glycoproteins and their recombinantly produced counterparts in different host cells. We will discuss in which ways recombinant glycoproteins can differ from their endogenous variants, and the functional consequences.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"357-379"},"PeriodicalIF":7.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional genomics in sugarcane breeding: key challenges and strategies. 功能基因组学在甘蔗育种中的应用：主要挑战和策略。

IF 7.7 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2026-05-01 Epub Date: 2026-01-27 DOI: 10.1080/07388551.2026.2614075

Rubab Shabbir, Talha Javed, Sheng-Ren Sun, Zhu-Qing Wang, Wei Zhang, San-Ji Gao, Qin-Nan Wang

{"title":"Functional genomics in sugarcane breeding: key challenges and strategies.","authors":"Rubab Shabbir, Talha Javed, Sheng-Ren Sun, Zhu-Qing Wang, Wei Zhang, San-Ji Gao, Qin-Nan Wang","doi":"10.1080/07388551.2026.2614075","DOIUrl":"10.1080/07388551.2026.2614075","url":null,"abstract":"Sugarcane, a leading source of sugar and bio-energy around the globe stands at the cross-road of genome complexity and agricultural innovation, offering the immense potential to fuel a sustainable future. Functional genomics with its precise identification and manipulation of genes could enable researchers unlock this potential and accelerate the breeding efforts. However, the polyploid genome of sugarcane with: high heterozygosity, high-repetitive DNA content, multiple copies of homo(eo)logous gene, epistatic interaction of alleles, etc., challenges the gene annotation, genome sequencing, genome editing, and phenotypic characterization. Similarly long breeding cycle, low transformation efficiency, time-consuming, and labor-intensive transformation methods further complicates the genome editing. Recent advances of functional genomics are transforming this scenario, such as current availability of reference genome \"R570,\" has provided a significant insight of genome architect and function. Genome wide association studies (GWAS)/genome selection (GS) are enhancing trait-mapping and prediction of breeding values to accelerate the breeding cycles. The current era of smart breeding with integrative bio-informatics, advance genome editing tools, i.e., CRISPR/Cas-systems (Cas-proteins, Cas-RNPs, d-Cas-RNPs, and CRISPRa/i), and high-throughput phenomics offers a significant approach to: overcome transformation bottlenecks, explore complex trait architect and address polyploidy challenges. Therefore, this review summarizes the key challenges and focuses on elaborating recent advances and suggests optimized strategies for future improvement in functional genomics of sugarcane breeding.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"419-439"},"PeriodicalIF":7.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146060691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic insights into plant-endophyte interactions: advancing understanding of mutualistic symbiosis and plant resilience. 植物-内生菌相互作用的蛋白质组学见解：推进对互利共生和植物恢复力的理解。

IF 7.7 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2026-05-01 Epub Date: 2026-03-22 DOI: 10.1080/07388551.2026.2618190

Shivam Chauhan, Poonam Kumari, Nikky Deepa, Jiya Chanotiya, Prabodh Kumar Trivedi, Akanksha Singh

{"title":"Proteomic insights into plant-endophyte interactions: advancing understanding of mutualistic symbiosis and plant resilience.","authors":"Shivam Chauhan, Poonam Kumari, Nikky Deepa, Jiya Chanotiya, Prabodh Kumar Trivedi, Akanksha Singh","doi":"10.1080/07388551.2026.2618190","DOIUrl":"10.1080/07388551.2026.2618190","url":null,"abstract":"Endophytic microorganisms are a vital part of the plant microbiome, contributing significantly to the plant's growth, development, and stress tolerance. Proteomics investigations have significantly enhanced our comprehension of the interactions between plants and endophytes, illuminating the complex molecular mechanisms that govern these mutually beneficial relationships. The review aims to integrate the latest developments in proteomic research concerning endophyte-plant interactions, emphasizing on elucidating the molecular mechanisms that underlie the benefit imparted to the host plant by the symbionts. The special focus of the review is to discuss the proteome level changes happening at the early recognition events, primary and secondary metabolism, signaling pathways, and defense mechanisms. By underscoring critical proteomic signatures, the review aspires to offer insights into how these interactions enhance plant health, increase stress resilience, and promote overall growth. The article discusses the potential applications of proteomics in agriculture and environmental sciences, emphasizing its role in crop resilience against biotic and abiotic stresses, optimizing biocontrol strategies, and improving nutrient use efficiency. The article also highlights that despite the advancements, critical gaps persist including the necessity for a deeper understanding of the temporal dynamics of proteomic responses, the specificity of protein-protein interactions, and the influence of environmental factors on the proteome induced by the endophytes. The review concludes by proposing future directions for proteomics research in plant-endophyte interactions for developing a more comprehensive understanding of the intricate molecular dialogues for developing a more sustainable and resilient agricultural systems.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"440-459"},"PeriodicalIF":7.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering non-ribosomal peptide synthesis: tuning the antibiotics engine of the microbial world. 工程非核糖体肽合成：调整微生物世界的抗生素引擎。

IF 7.7 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2026-05-01 Epub Date: 2026-03-02 DOI: 10.1080/07388551.2026.2615819

Lucy Butler, Ali Raza Awan, Tom Ellis, Muhammad Safwan Akram

引用次数: 0

Deciphering the mechanism of microalgal metabolite-protein interaction for polyhydroxyalkanoates production. 微藻代谢产物-蛋白质相互作用对聚羟基烷酸酯生产的机制解读。

IF 7.7 2区工程技术

Critical Reviews in Biotechnology Pub Date : 2026-04-26 DOI: 10.1080/07388551.2026.2652317

Adamu Yunusa Ugya, Zainab Kasim Mohammed, Mohammed Sani Jaafaru, Hui Chen, Qiang Wang

{"title":"Deciphering the mechanism of microalgal metabolite-protein interaction for polyhydroxyalkanoates production.","authors":"Adamu Yunusa Ugya, Zainab Kasim Mohammed, Mohammed Sani Jaafaru, Hui Chen, Qiang Wang","doi":"10.1080/07388551.2026.2652317","DOIUrl":"https://doi.org/10.1080/07388551.2026.2652317","url":null,"abstract":"The use of microalgal resources for bioplastic production has been hindered by resource flow limitations and large-scale production's economic viability. To address this limitation, this review intends to provide cost-effective methods for large-scale cultivation and surge in the accumulation of precursors for bioplastic production. The review shows how the engineering of metabolite-protein interaction favors optimization of growth conditions, nutrient availability, and harvesting techniques that increase the yield of bioplastic precursors from microalgae. The review shows that an in-depth understanding of metabolite-protein interaction for the biosynthesis of polyhydroxyalkanoates (PHA) can provide an important insight into the production of strains that will increase the bioplastic potential of microalgae. This research highlights the potential for optimizing metabolic pathways in microalgae to enhance the production of precursors linked to PHA, thus providing strategies that improve the efficiency and yield of these precursors from microalgae. The study also shows environmental stressors trigger post-translational modifications that enhance PHA production in microalgae. This post-translational change influences PHA synthesis by regulating metabolite-protein interaction that involves the activation of key enzymes in the biosynthetic pathway. Thus, indicating the benefit of manipulating these stressors to surge PHA accumulation in microalgae, thereby offering a sustainable solution for bioplastic production.","PeriodicalId":10752,"journal":{"name":"Critical Reviews in Biotechnology","volume":" ","pages":"1-17"},"PeriodicalIF":7.7,"publicationDate":"2026-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147764974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0