Critical reviews in analytical chemistry最新文献

{"title":"Recent Advances in Nanoparticle-Based Biosensors for <i>Campylobacter jejuni</i> Detection in Foods: Mechanisms, Designs, and Applications.","authors":"Sumeyra Savas, Seyed Mohammad Taghi Gharibzahedi","doi":"10.1080/10408347.2026.2723462","DOIUrl":"https://doi.org/10.1080/10408347.2026.2723462","url":null,"abstract":"<p><p><i>Campylobacter jejuni</i>, which is mostly transmitted through poultry products, is one of the most common bacterial causes of foodborne illness worldwide. Its low infectious dose and complex chicken matrices make reliable identification difficult, and conventional culture- and nucleic acid-based methods are still time-consuming and unsuitable for rapid or portable food safety surveillance. With a focus on food and food-chain-related matrices, this study critically assesses nanoparticle (NP)-based biosensors designed for <i>C. jejuni</i> detection. The functional roles of NPs, sensing mechanisms, nanomaterial classes, biosensing systems, application settings, comparative analytical performance, current issues, new trends, and methodological limitations are evaluated. NPs play function-driven roles in <i>C. jejuni</i> biosensing, including enzyme-mimetic or catalytic activity, target capture and separation, and signal amplification and transmission. These functions operate independently of tailored platform configurations and support immunosensor-, aptamer-, nucleic acid-, and whole-cell-based detection techniques. NP integration improved test sensitivity, signal robustness, and operational flexibility <i>via</i> immunomagnetic separation, plasmonic enhancement, catalytic signal creation, and dual-mode signal transduction techniques. Comparative studies revealed reproducible performance declines in complex food matrices compared to buffer systems, showing unresolved matrix effects and repeated pretreatment requirements. Several biosensors have been employed in milk, poultry, and model food systems. However, unified validation against reference procedures and validation utilizing naturally contaminated samples are still lacking. Although NP-based biosensors for <i>C. jejuni</i> constitute a well-established and varied analytical field, ongoing advancement will depend on increased reliability, standardized validation, and compliance with actual food safety regulations.</p>","PeriodicalId":10744,"journal":{"name":"Critical reviews in analytical chemistry","volume":" ","pages":"1-24"},"PeriodicalIF":7.7,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148988550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends and Advances in Analytical Methodologies for Favipiravir Determination Across Diverse Matrices.","authors":"Niralee J Malaviya, Hardik L Varu, Mrunal A Ambasana","doi":"10.1080/10408347.2026.2723461","DOIUrl":"https://doi.org/10.1080/10408347.2026.2723461","url":null,"abstract":"<p><p>Favipiravir, a broad-spectrum antiviral agent used against RNA viral infections, has been quantified in bulk drugs, pharmaceutical formulations, and biological matrices using various analytical approaches. This review critically evaluates reported methods, including UV-Vis spectrophotometry, spectrofluorimetry, electrochemical sensing, planar chromatography, and advanced chromatographic techniques. UV-Vis methods offer simplicity, low cost, and potential use of green solvents, whereas spectrofluorimetric methods provide enhanced sensitivity. Electrochemical sensors employing nanostructured materials and molecularly imprinted polymers enable rapid, portable, and sensitive detection, particularly in biological matrices. Planar chromatography provides cost-effective and high-throughput analysis with low solvent consumption, increasingly supported by densitometric and digital image-based quantification. RP-HPLC, UPLC, and LC-MS remain the most robust techniques, offering superior selectivity, resolution, and reproducibility for pharmaceutical, stability, and pharmacokinetic studies. Recent developments emphasize green analytical chemistry, chemometric-assisted analysis, quality-by-design optimization, and smartphone-based detection. However, incomplete validation and robustness assessment remain limitations in several reported methods. Overall, this review provides a comparative perspective on favipiravir analytical strategies and highlights future needs for standardized, sensitive, sustainable, and intelligent analytical methods.</p>","PeriodicalId":10744,"journal":{"name":"Critical reviews in analytical chemistry","volume":" ","pages":"1-44"},"PeriodicalIF":7.7,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148976037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smartphone-Integrated Laccase-like Nanozyme Colorimetric Sensors for Food Quality and Environmental Analysis: Mechanisms, Materials Design, and Analytical Challenges.","authors":"Saad Mohammed Almohya, Nasser S Alqahtani","doi":"10.1080/10408347.2026.2725850","DOIUrl":"https://doi.org/10.1080/10408347.2026.2725850","url":null,"abstract":"<p><p>The growing demand for portable, rapid, and cost-effective diagnostic methods has driven the advancement of smartphone-assisted colorimetric sensors. Among the various emerging substrates, colorimetric sensors based on laccase-like nanozymes have garnered significant interest due to their low cost, high stability, tunable catalytic properties, and environmentally friendly catalytic mechanisms. These nanozymes catalyze the oxidation of both phenolic and non-phenolic substrates by mediating a four-electron reduction of oxygen to water, without generating harmful hydrogen peroxide (H₂O₂). Significantly, they have developed a systematic design approach characterized by well-defined structure-activity relationships and well-established catalytic mechanisms. When combined with the advanced imaging and communication capabilities of smartphones, these nanozyme-enabled systems offer promising alternatives to traditional laboratory-based techniques. Herein, this study offers a critical analysis of recent advancements in smartphone-integrated colorimetric sensors that utilize laccase-like nanozymes. It specifically focuses on catalytic principles, the design of nanozyme materials, sensing methodologies, and their analytical applications. Significant emphasis is placed on various types of laccase-like materials and their potential uses in food safety and environmental analysis over the last three years. Additionally, structural optimization, electronic modulation, and bioinspired active site modifications aimed at enhancing enzyme-like activity have been explored. Finally, current challenges and future perspectives are critically discussed.</p>","PeriodicalId":10744,"journal":{"name":"Critical reviews in analytical chemistry","volume":" ","pages":"1-26"},"PeriodicalIF":7.7,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148963074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smartphone-Based Analytical Platforms: Portable Supplementary Tools for Seawater Monitoring of Nutrients, Heavy Metals, and Emerging Contaminants.","authors":"Jinling Pang, Luodan Chen, Shuyuan Huang, Hongzhe Chen, Yuanbiao Zhang, Kunning Lin","doi":"10.1080/10408347.2026.2729456","DOIUrl":"https://doi.org/10.1080/10408347.2026.2729456","url":null,"abstract":"<p><p>The increasing influx of nutrients, heavy metals, and emerging contaminants into marine ecosystems poses serious threats to environmental and human health. Conventional analytical techniques, however, remain largely laboratory-bound, expensive, and unsuitable for real-time field monitoring. In response, smartphone-based analytical platforms have rapidly emerged as a portable, low-cost, and user-friendly alternative for seawater analysis. Nevertheless, to the best of our knowledge, no comprehensive review has yet systematically integrated the three major pollutant categories-nutrients, heavy metals, and emerging contaminants-within the framework of smartphone-based detection specifically applied to seawater matrices. To fill this gap, this review evaluates representative studies on nutrient monitoring, heavy metal ion detection, and emerging contaminant analysis, covering literature published from 2017 to 2025. Despite substantial progress, major challenges persist, including seawater matrix interference, the tradeoff between portability and analytical performance, device-to-device variability, and the lack of standardized protocols. Future directions point toward artificial intelligence-assisted signal processing, hybrid multimodal platforms, cloud-connected monitoring networks, and commercialization. Integrating recent interdisciplinary developments, this review aims to provide a comprehensive reference and perspective regarding smartphone-based analytical platforms as a supplementary tool for routine marine environmental monitoring.</p>","PeriodicalId":10744,"journal":{"name":"Critical reviews in analytical chemistry","volume":" ","pages":"1-16"},"PeriodicalIF":7.7,"publicationDate":"2026-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148963138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toward High-Performance in Heavy Metal Detection: Mechanisms and Applications of Amplified Aptasensors.","authors":"Keshu Sun, Yueyao Tong, Jingbo Liu, Fengyun Li","doi":"10.1080/10408347.2026.2731400","DOIUrl":"https://doi.org/10.1080/10408347.2026.2731400","url":null,"abstract":"<p><p>Heavy metals (Pb, Cd, Hg, As) pose severe, persistent threats to ecological integrity and human health. Conventional detection methods are limited by insufficient sensitivity, cumbersome pretreatment, high operational requirements and costs, restricting rapid on-site detection in resource-limited settings. Aptasensors, with high specificity, excellent stability and low cost, have emerged as a promising alternative. To overcome the sensitivity bottleneck for trace/ultra-trace heavy metal detection, this review systematically dissects three mainstream signal amplification strategies for aptasensor-based heavy metal analysis: nanomaterial-enabled, DNAzyme-mediated, and nucleic acid logic circuit-based amplification. Notably, this review covers the application of AI-aptasensor integration for future heavy metal detection. We also summarize current research progress, prevailing challenges, and future optimization directions, aiming to advance practical high-performance aptasensors for real-world heavy metal monitoring.</p>","PeriodicalId":10744,"journal":{"name":"Critical reviews in analytical chemistry","volume":" ","pages":"1-30"},"PeriodicalIF":7.7,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148944371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spectroscopic Analysis for Rapid Identification and Characterization of WHO Priority Pathogen-<i>Acinetobacter baumannii</i>.","authors":"Abhishek Kandikatla, Karpagavalli Palpandi, Sonika Kar, Vidhya Das, Jaasmitha Popuri, Atul Kumar Jha, Rajapandiyan Panneerselvam, Sunil Chinnadurai, Jayaseelan Murugaiyan","doi":"10.1080/10408347.2026.2726514","DOIUrl":"https://doi.org/10.1080/10408347.2026.2726514","url":null,"abstract":"<p><p><i>Acinetobacter baumannii</i> (<i>A. baumannii</i>) has emerged as a major multidrug-resistant pathogen due to its ability to acquire antimicrobial resistance (AMR) and form robust biofilms. Unlike conventional methods, advanced spectroscopic techniques enable rapid detection and phenotyping of <i>A. baumannii</i> through distinct spectral signatures for accurate identification and characterization. Raman spectroscopy, including surface-enhanced Raman spectroscopy (SERS) and Fourier-transform infrared (FTIR) spectroscopy can differentiate resistant phenotypes and precise species identification through biomolecular fingerprints, while fluorescence spectroscopy can detects viability and antimicrobial responses using intrinsic or dye-based signals. Nuclear magnetic resonance (NMR) spectroscopy provides detailed metabolic profiling. Applied spectroscopic approaches such as hyperspectral imaging enables label-free detection and quantitative assessment of <i>A. baumannii</i> biofilms, while electrochemical impedance spectroscopy provides real-time monitoring of biofilm dynamics for rapid antimicrobial susceptibility testing (AST). This review highlights the potential role of spectroscopy in generating detailed biophysical and biochemical fingerprints of <i>A. baumannii</i>, supporting the transition from experimental studies to clinical diagnostics. Integration with machine learning (ML) further enhances high-throughput differentiation of colonizing and pathogenic <i>A. baumannii</i>.</p>","PeriodicalId":10744,"journal":{"name":"Critical reviews in analytical chemistry","volume":" ","pages":"1-19"},"PeriodicalIF":7.7,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148955172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transformative Potential of Laser Induced Breakdown Spectroscopy (LIBS) for the Analysis of Crime Scene Evidences: Application in Forensic Science.","authors":"Adwaith R, Saumya Shrivastava, Arvind Kumar Saxena","doi":"10.1080/10408347.2026.2725101","DOIUrl":"https://doi.org/10.1080/10408347.2026.2725101","url":null,"abstract":"<p><p>The strengthening and growth of forensic science is vital for a Nation's law enforcement and criminal justice system. Forensic agencies serve the criminal justice system by analyzing crime scene evidence, such as DNA, ballistic, gunshot residue, fingerprints, and questioned documents. With the constraint of minimal quantity of evidence recovered from crime scenes, there is a growing need for nondestructive analysis techniques that can preserve evidence for further testing or legal proceedings. The advent of Laser-Induced Breakdown Spectroscopy (LIBS) in the late twentieth century has revolutionized forensic science by providing a rapid, nondestructive, and highly accurate method for elemental analysis. LIBS provides an in-situ, faster, minimally-destructive method of elemental analysis. Integrating LIBS into the forensic science workflow can significantly improve evidence analysis's speed, accuracy, and efficiency. This article presents a comprehensive review of the transformative potential of LIBS in various forensic applications, emphasizing its advantages over traditional forensic methods. The article details the working principle of LIBS, which involves using a laser to create a plasma that emits light characteristic of the sample's elemental composition. This versatile technique is capable of analyzing various crime scene evidences and thus making it a fascinating tool for forensic scientists.</p>","PeriodicalId":10744,"journal":{"name":"Critical reviews in analytical chemistry","volume":" ","pages":"1-31"},"PeriodicalIF":7.7,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148873064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Metal-Organic Frameworks for Trace Element Detection in Water, Soil, and Food Systems.","authors":"Banshidhar, B Saravanakumar, S Parveen, Kavita Shah, Laxman Singh","doi":"10.1080/10408347.2026.2708858","DOIUrl":"https://doi.org/10.1080/10408347.2026.2708858","url":null,"abstract":"<p><p>Environmental pollution caused by hazardous trace metals, such as arsenic, mercury, uranium, and iron, presents significant risks to both human health and ecosystems, underscoring the need for advanced detection technologies. Metal-organic frameworks (MOFs), known for their high surface area, tunable porosity, and selective metal-binding properties, have emerged as promising candidates for detecting these hazardous metals in environmental matrices. This review explores the potential of MOFs in trace metal detection and their applications in monitoring pollutants in water, soil, and food. The review covers recent advancements in MOF-based sensors, focusing on their ability to provide rapid, sensitive, and selective detection methods, which are crucial for public health protection and industrial safety. Furthermore, it highlights the role of MOFs in addressing environmental concerns related to heavy metal contamination. The manuscript also examines key challenges faced by MOF-based sensing technologies, including issues of stability, scalability, and cost-efficiency, and proposes future research directions to overcome these barriers. By exploring the current state of research and identifying emerging opportunities, this review emphasizes the transformative potential of MOFs in environmental monitoring and hazardous material management. MOF-based technologies offer sustainable solutions for the detection and remediation of toxic contaminants, addressing the global challenges posed by heavy metal pollution in environmental contexts.</p>","PeriodicalId":10744,"journal":{"name":"Critical reviews in analytical chemistry","volume":" ","pages":"1-29"},"PeriodicalIF":7.7,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148873058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical Lateral Flow Assays: Signal-Generation Mechanisms, Material Architectures, and Translational Pathways for Quantitative Point-of-Care Diagnostics.","authors":"Thangapandi Kalyani","doi":"10.1080/10408347.2026.2719729","DOIUrl":"https://doi.org/10.1080/10408347.2026.2719729","url":null,"abstract":"<p><p>Lateral flow assays (LFAs) are widely used point-of-care diagnostic platforms because they are simple, rapid, low-cost, and easy to operate. The integration of electrochemical transduction into lateral flow platforms enables biorecognition events to be converted into measurable electrical signals. This approach supports portable, digitally readable, and user-friendly diagnostic testing. This review examines electrochemical lateral flow assays (eLFAs) through a design-oriented framework that connects signal-generation mechanisms, electrode materials, device architecture, and translational feasibility. It first discusses why traditional LFAs are constrained by optical readout and then summarizes the major electrochemical modes used in eLFAs, including amperometric, voltammetric, and impedimetric approaches. The principal signal-transduction strategies are then analyzed, including enzyme-mediated amplification, redox-active probes, metallic nanoparticle dissolution, direct electroactive labels, and label-free interfacial sensing. Particular emphasis is placed on how these mechanisms interact with material choice and physical integration strategy, including external electrodes, ex situ measurement formats, and fully integrated on-strip transducers. By comparing these approaches, this review highlights the tradeoffs between sensitivity, simplicity, manufacturability, and user operation. Finally, the major translational barriers that continue to limit real-world implementation are discussed, including reproducibility, biofouling, flow control, user-friendliness, regulatory validation, large-scale manufacturing, and sustainability.</p>","PeriodicalId":10744,"journal":{"name":"Critical reviews in analytical chemistry","volume":" ","pages":"1-45"},"PeriodicalIF":7.7,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148825734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Femtomolar Detection to Court-Ready Evidence: Nanomaterial-Enhanced Forensic Analytics, Validation Challenges, and the Road to 2030.","authors":"Picheswara Rao Polu","doi":"10.1080/10408347.2026.2722150","DOIUrl":"https://doi.org/10.1080/10408347.2026.2722150","url":null,"abstract":"<p><p>Nanomaterial-enhanced analytical methods have emerged as transformative platforms in forensic science, addressing critical sensitivity, selectivity, and operational limitations of conventional techniques across trace evidence, toxicology, biological fluid analysis, and nucleic acid profiling. Gold nanoparticles, quantum dots, carbon-based nanomaterials, and magnetic nanoparticles have collectively enabled detection capabilities at femtomolar to attomolar concentrations, multiplexed immunoassay formats, magnetically assisted sample preparation from degraded biological matrices, and enhanced PCR amplification from inhibitor-rich forensic specimens. Surface-enhanced Raman scattering, fluorescence-based transduction, and electrochemical sensing at nanocomposite electrode surfaces have each demonstrated performance profiles that substantially exceed conventional forensic analytical benchmarks. Despite these advances, the translation of nanomaterial platforms into accredited forensic casework remains constrained by nanoparticle aggregation instability, batch-to-batch synthesis variability, matrix-dependent signal suppression, and the absence of universally adopted validation frameworks governing limit of detection determination, measurement uncertainty quantification, and proficiency testing for nanomaterial-specific analytical modalities. Emerging innovations including portable handheld SERS devices, blockchain-integrated chain-of-custody architectures, molecularly imprinted polymer nanoparticle probes, and AI-augmented chemometric classification frameworks are collectively advancing the field toward real-time, field-deployable forensic analysis. Sustained progress requires parallel investment in international standardization, ethical governance of ultra-sensitive biological surveillance capabilities, and equitable access infrastructure ensuring that nanomaterial-enabled forensic precision serves justice systems globally.</p>","PeriodicalId":10744,"journal":{"name":"Critical reviews in analytical chemistry","volume":" ","pages":"1-28"},"PeriodicalIF":7.7,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148812080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}