Connective Tissue Research最新文献_第3页

Application of tissue engineering approaches in osteoarthritis. 组织工程技术在骨关节炎中的应用。

IF 2.8 4区医学

Connective Tissue Research Pub Date : 2025-05-31 DOI: 10.1080/03008207.2025.2509135

Liru Wen, Fatemeh Safari, Zhen Li, Martin J Stoddart

{"title":"Application of tissue engineering approaches in osteoarthritis.","authors":"Liru Wen, Fatemeh Safari, Zhen Li, Martin J Stoddart","doi":"10.1080/03008207.2025.2509135","DOIUrl":"https://doi.org/10.1080/03008207.2025.2509135","url":null,"abstract":"This review article examines the application of tissue engineering approaches in the treatment of osteoarthritis (OA), a complex joint disease characterized by tissue crosstalk and inflammation. The article covers preclinical testing platforms, including long-term in vitro studies, ex vivo models with osteochondral explants, and in vivo animal studies. It highlights the advantages and limitations of these models in evaluating tissue-engineered constructs for OA repair and focusses on cartilage specific treatments and resurfacing. The review also explores focal damage approaches such as autologous cultured chondrocytes and Autologous Matrix-Induced Chondrogenesis, which have shown improved patient outcomes. Additionally, it discusses natural and synthetic biomaterials used in cartilage repair, emphasizing the need for combining materials to enhance therapeutic efficacy. The importance of long-term studies in large animal models is underscored to develop effective strategies for cartilage repair. This minireview explores various approaches aimed at effectively addressing and repairing cartilage damage, covering preclinical testing platforms, cartilage resurfacing methods, and tissue engineering (TE) clinical trials. It also highlights challenges in developing future cartilage repair therapies.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":" ","pages":"1-8"},"PeriodicalIF":2.8,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The beneficial and detrimental effects of exercise and unloading on OA progression after anterior cruciate ligament injury. 运动和卸载对前交叉韧带损伤后骨性关节炎进展的有益和有害影响。

IF 2.8 4区医学

Connective Tissue Research Pub Date : 2025-05-23 DOI: 10.1080/03008207.2025.2507858

Yu-Yang Lin, Blaine A Christiansen

{"title":"The beneficial and detrimental effects of exercise and unloading on OA progression after anterior cruciate ligament injury.","authors":"Yu-Yang Lin, Blaine A Christiansen","doi":"10.1080/03008207.2025.2507858","DOIUrl":"https://doi.org/10.1080/03008207.2025.2507858","url":null,"abstract":"Injury of the anterior cruciate ligament (ACL) is a common sports injury that can lead to post-traumatic osteoarthritis (PTOA) within 10-20 years. Surgical ACL reconstruction is often performed several weeks or months after injury, and this period between injury and ACL reconstruction may be a critical time for determining the risk of long-term PTOA progression. However, few (if any) studies in human patients have investigated the long-term effects of exercise or unloading between ACL injury and surgery. Early mobilization is often recommended to maintain range of motion and muscle strength, which are beneficial for positive outcomes of ACL reconstruction, but it is unknown what effects early mobilization or unloading have on long-term PTOA progression. In preclinical animal studies, a brief period of joint unloading immediately after ACL injury significantly decreased osteophyte formation and articular cartilage degeneration, while longer-term non-weightbearing caused muscle atrophy and articular cartilage degradation. Similarly, preclinical studies have shown that different intensities of exercise after knee injury can have divergent effects on PTOA development. Low intensity exercise was protective against joint degeneration, while higher intensity exercise accelerated PTOA progression. The beneficial or detrimental effects of exercise and unloading following ACL injury are likely dependent on the timing, duration, and intensity of these biomechanical interventions. This review summarizes the effects of these biomechanical interventions after ACL injury in both humans and animal models, with the goal of informing therapeutic and rehabilitation strategies for slowing or preventing PTOA progression after injury.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":" ","pages":"1-7"},"PeriodicalIF":2.8,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response of cartilage explants to LPS cultured in normoxic and hypoxic conditions is modulated by Spirulina: implications for exercise recovery in vivo. 在常氧和缺氧条件下培养的软骨外植体对LPS的反应是由螺旋藻调节的：对体内运动恢复的影响。

IF 2.8 4区医学

Connective Tissue Research Pub Date : 2025-05-23 DOI: 10.1080/03008207.2025.2507239

Nadia Golestani, Wendy Pearson

{"title":"Response of cartilage explants to LPS cultured in normoxic and hypoxic conditions is modulated by Spirulina: implications for exercise recovery in vivo.","authors":"Nadia Golestani, Wendy Pearson","doi":"10.1080/03008207.2025.2507239","DOIUrl":"https://doi.org/10.1080/03008207.2025.2507239","url":null,"abstract":"Exercise-induced inflammation and free radical production are crucial for recovery, yet excess inflammation poses risks to equine athletes, leading to conditions like arthritis. Spirulina, recognized for its antioxidant and anti-inflammatory properties, could mitigate degenerative diseases without hindering post-exercise recovery. This study investigates Spirulina's direct impact on cartilage responses to LPS-induced inflammation in normoxic and hypoxic conditions, focusing on outcomes relevant to cartilage matrix turnover and exercise-induced inflammation. Spirulina underwent simulated digestion and liver metabolism, yielding a simulated biological extract (SPsim). In the normoxic experiment, porcine cartilage explants were cultured with SPsim (0, 30, or 90 μg/mL) for 72 h after 24 h in basal media, with LPS (0 or 10 μg/mL) added for the final 48 h. The hypoxic experiment mirrored this, with explants transferred to a hypoxia chamber for the final 48 h. Media samples collected at 0, 24, and 48 h were analyzed for biomarkers related to cartilage turnover (GAG), and exercise-induced inflammation (IL-6 and NO). Cell viability, assessed by live:dead staining, remained > 97% and unaffected by oxygen tension. In normoxic conditions, SPsim (30 μg/mL) significantly reduced GAG release at 48 h. Under hypoxia, SPsim (30 and 90 μg/mL) inhibited LPS-induced GAG release. SPsim (90 μg/mL) increased IL-6 and NO production in LPS-stimulated explants in normoxia, and a similar effect was observed with the lower SPsim dose (30 μg/mL) in hypoxic conditions. These results suggest that Spirulina may enhance cartilage mediators, potentially promoting healthy cartilage turnover during exercise recovery.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":" ","pages":"1-14"},"PeriodicalIF":2.8,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PELI1 silencing delays intervertebral disc degeneration by impeding nucleus pulposus cell death. PELI1沉默通过阻碍髓核细胞死亡延缓椎间盘退变。

IF 2.8 4区医学

Connective Tissue Research Pub Date : 2025-05-23 DOI: 10.1080/03008207.2025.2508841

Xiaodong Wei, Chao Yu, Jingjie Wang

{"title":"PELI1 silencing delays intervertebral disc degeneration by impeding nucleus pulposus cell death.","authors":"Xiaodong Wei, Chao Yu, Jingjie Wang","doi":"10.1080/03008207.2025.2508841","DOIUrl":"https://doi.org/10.1080/03008207.2025.2508841","url":null,"abstract":"Purpose: Intervertebral disc degeneration (IDD) is a spinal condition that causes low back pain. Pellino E3 ubiquitin protein ligase 1 (PELI1) expression reportedly correlates with inflammation and cell death. This study aimed to determine its potential role in IDD.Methods: Cell counting kit-8 assay, 5-ethynyl-2'-deoxyuridine staining, senescence-associated β-galactosidase staining, morphological observation, lactate dehydrogenase (LDH) release assay, quantitative reverse transcriptase polymerase chain reaction, and western blotting were used to examine the effect of PELI1 on tumor necrosis factor alpha (TNF-α)-induced human primary nucleus pulposus cells (hNPCs).Results: PELI1 was highly expressed in TNF-α-treated hNPCs. TNF-α treatment notably reduced hNPCs viability and proliferation, but enhanced senescence (elevated p16 and p21 expression), extracellular matrix degeneration (reduced collagen II and aggrecan expression and upregulated matrix metallopeptidase-13 and a disintegrin and metalloproteinase with thrombospondin type 1 motifs-5 expression), nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing-3 (NLRP3) inflammasome formation (enhanced NLRP3, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), and cleaved caspase 1 expression), pyroptosis (elevated cleaved gasdermin D expression), LDH release, and inflammatory cytokine release (high mobility group box 1, interleukin (IL)-1β, and IL-18). These effects were distinctly reduced by PELI1 silencing but enhanced by its overexpression. Interestingly, the effects triggered by PELI1 silencing were partially reversed by ASC overexpression.Conclusions: PELI1 May promote IDD progression by expediting nucleus pulposus cell death and participates in the inflammatory response regulated by the NLRP3 inflammasome in nucleus pulposus cells. These suggest PELI1 as a potential therapeutic target for the treatment of IDD.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":" ","pages":"1-13"},"PeriodicalIF":2.8,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation of the mechanosensitive ion channels TRPV4 and PIEZO1 downregulates key regulatory systems in the chondrocyte mechanome. 机械敏感离子通道TRPV4和PIEZO1的激活下调了软骨细胞机械机制中的关键调控系统。

IF 2.8 4区医学

Connective Tissue Research Pub Date : 2025-05-21 DOI: 10.1080/03008207.2025.2498512

Daniel R Palmer, Robert Nims, Bo Zhang, Farshid Guilak

{"title":"Activation of the mechanosensitive ion channels TRPV4 and PIEZO1 downregulates key regulatory systems in the chondrocyte mechanome.","authors":"Daniel R Palmer, Robert Nims, Bo Zhang, Farshid Guilak","doi":"10.1080/03008207.2025.2498512","DOIUrl":"10.1080/03008207.2025.2498512","url":null,"abstract":"Background: Chondrocytes, the only native cell type in cartilage, use mechanosensitive ion channels such as Transient Receptor Potential Vanilloid 4 (TRPV4) and PIEZO1 to transduce mechanical forces into transcriptomic changes that regulate cell behavior under both physiologic and pathologic conditions. Recent work has identified and characterized the differentially expressed genes (DEGs) that are upregulated following TRPV4 or PIEZO1 activation, but the transcriptomic systems downregulated by these ion channels also represent an important aspect of the chondrocyte regulatory process that remains poorly studied.Methods: Here, we utilized previously established bulk RNAsequencing libraries to analyze the transcriptomes downregulated by activation of TRPV4 and PIEZO1 through differential gene expression analysis (using DESeq2), Gene Ontology, RT-qPCR, and Weighted Gene Correlation Network Analysis (WGCNA).Results: TRPV4 and PIEZO1 activations downregulated largely unique sets of DEGs, though the set of DEGs downregulated by TRPV4 exhibited a notable overlap with genes downregulated by treatment with inflammatory mediator Interleukin-1 (IL-1). The DEG set downregulated by PIEZO1 activation included genes associated with the G2/M cell cycle checkpoint, a system that checks cells for DNA damage prior to entry into mitosis, and this result was confirmed with RT-qPCR. WGCNA revealed modules of gene regulation negatively correlated with TRPV4, PIEZO1, and IL-1, outlining how these downregulated DEGs may interact to form gene regulatory networks (GRNs).Conclusion: This study complements previous work in describing the full mechanosensitive transcriptome (or \"mechanome\") of differential gene expression in response to activation of mechanosensitive ion channels TRPV4 and PIEZO1 Q2 and suggests potential avenues for future therapeutic treatment design.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":" ","pages":"1-24"},"PeriodicalIF":2.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12237534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial transcriptomic applications in orthopedics. 空间转录组学在骨科中的应用。

IF 2.8 4区医学

Connective Tissue Research Pub Date : 2025-05-10 DOI: 10.1080/03008207.2025.2501703

Thomas L Jenkins, Jasper H N Yik, Dominik R Haudenschild

{"title":"Spatial transcriptomic applications in orthopedics.","authors":"Thomas L Jenkins, Jasper H N Yik, Dominik R Haudenschild","doi":"10.1080/03008207.2025.2501703","DOIUrl":"https://doi.org/10.1080/03008207.2025.2501703","url":null,"abstract":"Purpose: This review highlights the transformative impact of spatial transcriptomics on orthopedic research, focusing on its application in deciphering intricate gene expression patterns within musculoskeletal tissues.Methods: The paper reviews literature for spatial transcriptomic methods, specifically 10X Visium, 10X Xenium, seqFISH+, MERFISH, NanoString GeoMx DSP, used on musculoskeletal tissues (cartilage, joints, bone, tendon, ligament, and synovium).Results: Searches identified 29 published manuscripts reporting spatial transcriptomic data in cartilage, bone, tendon, synovium, and intervertebral disc. Most publications of spatial transcriptomic data are from tendon and synovium. 10X Visium has been used in 22 of the 29 papers identified. Spatial transcriptomics has been used to identify novel cell populations and cell signaling pathways that regulate development and disease.Conclusions: Imaging-based spatial transcriptomic methods may be better for hypothesis testing as they generally have subcellular resolution but sequence fewer genes. Sequencing methods may be better for untargeted, shotgun approaches that can generate useful hypotheses from the spatial data from unimpaired tissue sections. Spatial transcriptomic methods could become useful for clinical diagnostics and precision medicine approaches.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":" ","pages":"1-12"},"PeriodicalIF":2.8,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanical insights into fat pads: a comparative study of infrapatellar and suprapatellar fat pads in osteoarthritis. 脂肪垫的机械洞察：骨关节炎中髌下和髌上脂肪垫的比较研究。

IF 2.8 4区医学

Connective Tissue Research Pub Date : 2025-05-09 DOI: 10.1080/03008207.2025.2502591

Sofia Pettenuzzo, Alice Berardo, Elisa Belluzzi, Assunta Pozzuoli, Pietro Ruggieri, Emanuele Luigi Carniel, Chiara Giulia Fontanella

{"title":"Mechanical insights into fat pads: a comparative study of infrapatellar and suprapatellar fat pads in osteoarthritis.","authors":"Sofia Pettenuzzo, Alice Berardo, Elisa Belluzzi, Assunta Pozzuoli, Pietro Ruggieri, Emanuele Luigi Carniel, Chiara Giulia Fontanella","doi":"10.1080/03008207.2025.2502591","DOIUrl":"https://doi.org/10.1080/03008207.2025.2502591","url":null,"abstract":"Objective: Osteoarthritis (OA) is the most common musculoskeletal disorder, primarily affecting knee joints and causing pain and disability. The infrapatellar (IFP) and the suprapatellar (SFP) fat pad are knee adipose tissues that play essential mechanical roles during articular activity but are also sources of adipokines and cytokines, contributing to OA progression. For this reason, this work aims to provide new insights into IFP and SFP implications in knee OA.Materials and methods: IFP and SFP tissue mechanical properties were studied through compression, indentation and shear mechanical tests performed on samples collected from patients who underwent total knee arthroplasty surgery due to end-stage OA. The energy loss, peak stress, and initial and final elastic moduli were calculated from the unconfined compression tests. The time-dependent response, evaluated in terms of equilibrium relative stiffness, was computed from stress-relaxation loading conditions. Considering shear tests, they provided strain-energy dissipation density, peak shear stress, and the shear moduli.Results: Experimental results showed the typical adipose tissue mechanics features: non-linear stiffening with strain and time-dependent response. Experimental results showed that OA IFP is stiffer than OA SFP, indeed IFP final compression elastic modulus was greater than the SFP (84.43 kPa vs 35.54 kPa respectively) (p = 0.042). Regarding the viscoelastic properties they were comparable: the equilibrium relative stiffness was reported as 0.13 for IFP and 0.11 for SFP (p = 0.026).Conclusions: These outcomes provide new insights into the OA influence on knee mechanics and lay the basis for developing computational tools to improve knee prosthesis design.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":" ","pages":"1-12"},"PeriodicalIF":2.8,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cross-regulation of inflammation and metabolic mechanisms in osteoarthritis: recent advances bridging the gap to novel treatments. 骨关节炎炎症和代谢机制的交叉调节：最近的进展弥合了新治疗方法的差距。

IF 2.8 4区医学

Connective Tissue Research Pub Date : 2025-05-07 DOI: 10.1080/03008207.2025.2500530

Yousef Abu-Amer

{"title":"Cross-regulation of inflammation and metabolic mechanisms in osteoarthritis: recent advances bridging the gap to novel treatments.","authors":"Yousef Abu-Amer","doi":"10.1080/03008207.2025.2500530","DOIUrl":"10.1080/03008207.2025.2500530","url":null,"abstract":"Osteoarthritis (OA) is a debilitating degenerative disease of the joints and one of the most prevalent joint disorders affecting millions of individuals worldwide. This disease is highlighted by significant morbidity owing to encumbering joint pain and functional impairment. OA ensues following disruption of normal homeostasis in the joint resulting from aging, metabolic changes, or as a consequence of joint injury (referred to as post-traumatic OA). These processes are largely driven by low-grade inflammation that gradually compromises the anabolic and protective activities of joint resident cells including chondrocytes, synovial fibroblasts (SFs) and immune cells. Ample research suggests that the process of cartilage deterioration is the endpoint of complex pathologic processes culminating with synovitis, subchondral bone sclerosis, osteophyte formation, aberrant remodeling, and ultimately articular cartilage degradation. There remains a great need for identifying early markers and a \"window of opportunity\" to enable timely interventions in OA. However, this effort is hampered by the complex nature of the disease and its comorbidities. Joint holistic approaches using recent unbiased multi-omic tools are currently at the forefront promising better understanding of OA development. Currently, there are no meaningful disease-modifying drugs to treat OA, with surgical procedures as the ultimate effective intervention for end stage OA patients. The disability, pain, and surgical costs associated with OA management position this disease among the costliest and onerous for our society. This mini review will highlight advances in the last two decades and major obstacles limiting progress in OA research with particular emphasis on metabolic and inflammatory comorbidities.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":" ","pages":"1-6"},"PeriodicalIF":2.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peripheral nerve repair using olfactory ensheathing and stem cells within a vein graft. 利用嗅鞘和静脉移植干细胞修复周围神经。

IF 2.8 4区医学

Connective Tissue Research Pub Date : 2025-05-01 Epub Date: 2025-04-06 DOI: 10.1080/03008207.2025.2466693

Brent A McMonagle

{"title":"Peripheral nerve repair using olfactory ensheathing and stem cells within a vein graft.","authors":"Brent A McMonagle","doi":"10.1080/03008207.2025.2466693","DOIUrl":"10.1080/03008207.2025.2466693","url":null,"abstract":"Background: The aim of this study was to assess nerve regeneration in vein grafts filled with olfactory ensheathing cells (OECs) or olfactory stem cells (ONSs) in a 15 mm gap male DA rat sciatic nerve model versus autografts.Methods: The control group (NG) received a nerve graft reversed and sutured into the 15 mm gap; all of the animals in the five experimental groups received a vein graft to bridge the 15 mm gap filled with extracellular matrix (ECM- MatrigelTM) only (VG); ECM with rat OECs suspended in ECM (VG + rOECs); ECM with human OECs (with Cyclosporin postoperatively to prevent graft rejection) (VG + hONS (c)); ECM only (with Cyclosporin postoperatively as a control for the previous group) (VG (c)); and ECM with rat ONSs within the vein grafts (VG + rONS). After 12 weeks ±4 days, electrophysiological analysis (latency and amplitude) and histological assessment of axon counts (immunohistochemistry with neurofilament [NF] stain) were undertaken.Results: Group VG + rOECs had the lowest latency results, NG had the highest amplitude results, and groups NG and VG + rOECs had significantly higher axon counts.Conclusions: The results trended toward the VG + rOECs and NG groups having the most successful electrophysiology results and axon counts. Incorporating OECs into vein grafts may be a viable alternative to nerve grafts for peripheral nerve repair.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":" ","pages":"149-160"},"PeriodicalIF":2.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LncRNA SNHG7 inhibits apoptosis and proliferation of osteoarthritis cells induced by IL-β through sponging miR-146b. LncRNA SNHG7通过海绵miR-146b抑制IL-β诱导的骨关节炎细胞凋亡和增殖。

IF 2.8 4区医学

Connective Tissue Research Pub Date : 2025-05-01 Epub Date: 2025-04-10 DOI: 10.1080/03008207.2025.2487470

Naikai Lin, Zehui Song, Bitao Ma, Peng Wang

{"title":"LncRNA SNHG7 inhibits apoptosis and proliferation of osteoarthritis cells induced by IL-β through sponging miR-146b.","authors":"Naikai Lin, Zehui Song, Bitao Ma, Peng Wang","doi":"10.1080/03008207.2025.2487470","DOIUrl":"10.1080/03008207.2025.2487470","url":null,"abstract":"Purpose: We probed the roles of SNHG7, miR-146b, PCBP1, and IL-β in the development of osteoarthritis (OA).Materials and methods: OA models were established using anterior cruciate ligaments, and chondrocytes were obtained from mouse cartilage tissue. Cells were treated with 10 ng/ml Il-1β. RT-qPCR was used to detect the expression of SNHG7, miR-146b, PCBP1, and IL-β in tissues and cells. Safranin-O/Fast Green staining was performed to analyze the cartilage damage in each group of mice.Results: SNHG7 and PCBP1 expressions were down-regulated, and miR-146b expression was up-regulated in OA tissue and IL-1β-treated chondrocytes compared to normal cartilage tissue and chondrocytes. Forced SNHG7 expression improved cartilage structure, enhanced proliferative viability of chondrocytes, and inhibited apoptosis and IL-1β release in IL-1β-treated chondrocytes in OA mice. In contrast, miR-146b upregulation decreased proliferative viability and promoted apoptosis and IL-1β release in chondrocytes. Rescue assays showed that miR-146b attenuated the protective effects of SNHG7 on apoptosis and inflammation in IL-1β-treated chondrocytes, and activation of PCBP1 expression significantly inhibited the cytotoxic effects of miR-146b. Mechanistically, SNHG7 acted as a competitive endogenous RNA by targeting miR-146b to promote the expression of PCBP1.Conclusions: This study confirms that SNHG7 inhibits IL-1β-mediated inflammatory responses in chondrocytes via the miR-146b/PCBP1 axis, thereby suppressing IL-1β-induced OA.","PeriodicalId":10661,"journal":{"name":"Connective Tissue Research","volume":" ","pages":"190-203"},"PeriodicalIF":2.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0