Clinics最新文献

{"title":"Oxycodone enhances antitumor effect of paclitaxel on human breast cancer SKBR3 cells in vitro.","authors":"Fangfang Liu, Hongmei Yuan, Chenyang Xu, Mingjie Mao, Shanwu Feng","doi":"10.1016/j.clinsp.2024.100458","DOIUrl":"10.1016/j.clinsp.2024.100458","url":null,"abstract":"<p><strong>Background: </strong>The influences of Oxycodone (OXY) combined with Paclitaxel (PTX) on breast cancer cells are unclear. The present study aimed to examine the effects of OXY combined with PTX on the proliferation, apoptosis, and migration of human breast cancer SKBR3 cells and the underlying mechanism.</p><p><strong>Methods: </strong>The proliferation, apoptosis and invasion of SKBR3 cells were assessed by CCK-8, colony formation assay, flowcytometric, Transwell assay and scratch assays, respectively. In addition, Western blotting was used to detect the expression of related proteins in these cells. The autophagic bodies were observed under a transmission electron microscope.</p><p><strong>Results: </strong>OXY (0.25, 0.5 and 1 mM) significantly inhibited the viability, colony-forming, migration, and invasion of SKBR3 cells as compared to the control group. Furthermore, OXY (0.25, 0.5 and 1 mM) markedly induced the apoptosis of SKBR3 cells and the levels of apoptosis-related proteins. In addition, OXY (0.25, 0.5 and 1 mM) and PTX inhibited the proliferation of SKBR3 cells synergistically as compared to PTX group in vitro. Moreover, OXY (0.25, 0.5 and 1 mM) significantly elevated the PTX-induced apoptosis in SKBR3 cells via downregulating the expression of N-cadherin, Becline-1 LC3-Ⅱ, p-Akt and p-mTOR and upregulating E-cadherin expression. Compared with the control group, OXY (1 mM) treatment induced autophagy in SKBR3 cells.</p><p><strong>Conclusions: </strong>The present study indicates that OXY can enhance the antitumor effect of PTX on breast cancer in vitro. Hence, the combination of OXY with PTX may serve as a potential strategy for the treatment of breast cancer.</p>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"79 ","pages":"100458"},"PeriodicalIF":2.2,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11342192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141859225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of prognostic nomogram based on the SEER database for esophageal cancer patients.","authors":"Xiying Cao, Bingqun Wu, Shaoming Guo, Weixiang Zhong, Zuxiong Zhang, Hui Li","doi":"10.1016/j.clinsp.2024.100433","DOIUrl":"10.1016/j.clinsp.2024.100433","url":null,"abstract":"<p><p>Currently, the incidence of esophageal cancer continues to rise around the world. Because of its good early prognosis, it is of great significance to establish an effective model for predicting the survival of EC patients. The purpose of this study was to predict survival after diagnosis in Esophageal Cancer (EC) patients by constructing a valid clinical nomogram. In this study, 5037 EC patient samples diagnosed from 2010 to 2015 were screened by accessing the SEER database, and 8 independent prognostic factors were screened by various methods, and Cox multivariate regression was included to construct a prognostic model and nomogram for esophageal cancer. to estimate esophageal cancer recurrence and overall survival. Calibration of the nomogram predicted probabilities of 1-year, 3-year and 5-year survival probability, which were closely related to actual survival. In conclusion, this study validated that the column-line graphical model can be considered an individualized quantitative tool for predicting the prognosis of patients with EC in order to assist clinicians in making therapeutic decisions.</p>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"79 ","pages":"100433"},"PeriodicalIF":2.2,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11334687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141854952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Albumin corrected anion gap for predicting in-hospital death among patients with acute myocardial infarction: A retrospective cohort study.","authors":"Zhouzhou Lu, Yiren Yao, Yangyang Xu, Xin Zhang, Jing Wang","doi":"10.1016/j.clinsp.2024.100455","DOIUrl":"10.1016/j.clinsp.2024.100455","url":null,"abstract":"<p><strong>Objective: </strong>To explore the relationship between Anion Gap (AG), Albumin Corrected AG (ACAG), and in-hospital mortality of Acute Myocardial Infarction (AMI) patients and develop a prediction model for predicting the mortality in AMI patients.</p><p><strong>Methods: </strong>This was a retrospective cohort study based on the Medical Information Mart for Intensive Care (MIMIC)-Ⅲ, MIMIC-IV, and eICU Collaborative Study Database (eICU). A total of 9767 AMI patients who were admitted to the intensive care unit were included. The authors employed univariate and multivariable cox proportional hazards analyses to investigate the association between AG, ACAG, and in-hospital mortality; p < 0.05 was considered statistically significant. A nomogram incorporating ACAG and clinical indicators was developed and validated for predicting mortality among AMI patients.</p><p><strong>Results: </strong>Both ACAG and AG exhibited a significant association with an elevated risk of in-hospital mortality in AMI patients. The C-index of ACAG (C-index = 0.606) was significantly higher than AG (C-index = 0.589). A nomogram (ACAG combined model) was developed to predict the in-hospital mortality for AMI patients. The nomogram demonstrated a good predictive performance by Area Under the Curve (AUC) of 0.763 in the training set, 0.744 and 0.681 in the external validation cohort. The C-index of the nomogram was 0.759 in the training set, 0.756 and 0.762 in the validation cohorts. Additionally, the C-index of the nomogram was obviously higher than the ACAG and age shock index in three databases.</p><p><strong>Conclusion: </strong>ACAG was related to in-hospital mortality among AMI patients. The authors developed a nomogram incorporating ACAG and clinical indicators, demonstrating good performance for predicting in-hospital mortality of AMI patients.</p>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"79 ","pages":"100455"},"PeriodicalIF":2.2,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11334651/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141854951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance of triggers in detecting hospitalizations related to drug-induced respiratory disorders in older adults: A pilot cross-sectional study.","authors":"Geovana Schiavo, Marcela Forgerini, Fabiana Rossi Varallo, Bruna Carolina Corrêa, Maisa Cabete Pereira Salvetti, Patrícia de Carvalho Mastroianni","doi":"10.1016/j.clinsp.2024.100449","DOIUrl":"10.1016/j.clinsp.2024.100449","url":null,"abstract":"<p><strong>Background: </strong>There is no gold-standard trigger for detecting drug-induced respiratory disorders, a type of Adverse Drug Event (ADE) with high morbimortality, particularly in older people.</p><p><strong>Objective: </strong>To propose and evaluate the performance of triggers for detecting hospitalizations related to drug-induced respiratory disorders in older people.</p><p><strong>Methods: </strong>A pilot cross-sectional study was conducted with older people (age ≥ 60) admitted to a Brazilian hospital. Electronic chart documentation was screened using ICD-10 codes; Global Trigger Tool (GTT); and drugs potentially associated with respiratory disorders. A chart and medication review were conducted to perform the causality assessment using the instrument developed by the World Health Organization. The performance of triggers was evaluated by the Positive Predictive Value (PPV), with values ≥ 0.20 indicating good performance.</p><p><strong>Results: </strong>Among 221 older people, 72 were eligible. Potential drug-induced dyspnea and/or cough were detected in six older people (6/72), corresponding to a prevalence of 8.3 %. The overall PPV of the triggers was 0.14, with abrupt medication stop (PPV = 1.00), codeine (PPV = 1.00), captopril (PPV = 0.33), and carvedilol (PPV = 0.33) showing good performance. Two triggers were proposed for detecting therapeutic ineffectiveness associated with respiratory disorders: furosemide (PPV = 0.23) and prednisone (PPV = 0.20).</p><p><strong>Conclusion: </strong>The triggers enabled the identification that one in 12 hospitalizations was related to drug-induced respiratory. Although good performance was observed in the application of triggers, additional investigations are needed to assess the feasibility of incorporating them into clinical practice for the screening, detection, management, and reporting of these ADEs, which are considered to be underreported and difficult to detect.</p>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"79 ","pages":"100449"},"PeriodicalIF":2.2,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11332799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141787455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of the antiviral medications azvudine and nirmatrelvir-ritonavir in treating COVID-19 in patients with hematological malignancies.","authors":"Zheng Zeng, Fangyuan Li, Mingli Zhong, Ling Zhu, Wei Chen, Xiaotao Wang","doi":"10.1016/j.clinsp.2024.100406","DOIUrl":"10.1016/j.clinsp.2024.100406","url":null,"abstract":"<p><strong>Background: </strong>Patients with Hematological Malignancies (HM) are at a high risk of mortality from Coronavirus disease 2019 (COVID-19). The available antivirals were different between China and other countries. In China, azvudine was obtained for emergency use to treat adult COVID-19 patients with moderate symptoms in July 2022. While nirmatrelvir-ritonavir was well-known and used in many countries. The purpose of the present study was to assess whether there was any difference in the efficacy and safety of the two drugs.</p><p><strong>Methods: </strong>This study was a prospective observational study of patients with HM who developed COVID-19. Patients were divided into three treatment groups: nirmatrelvir-ritonavir, azvudine, and observation. Treatment outcomes, first nucleic acid test negative time, hospitalization time, and the conversion rate of mild or moderate disease to severe disease were recorded.</p><p><strong>Results: </strong>First nucleic acid test negative time (23.5 days vs. 34 days, p = 0.015), hospitalization time (p = 0.015), and conversion rate (31.8 % vs. 8 %, p = 0.046) were statistically different between the nirmatrelvir-ritonavir and observation groups. First nucleic acid test negative time (20 days vs. 34 days, p = 0.009) and hospitalization time (p = 0.026) were statistically different between the azvudine and observation groups. ECOG score and liver disease were significantly associated with the conversion rate from mild or moderate disease to severe disease using multivariate analysis (p < 0.05).</p><p><strong>Conclusions: </strong>The authors found no significant differences existed in outcome measures between patients with HM and COVID-19 who were treated with nirmatrelvir-ritonavir or azvudine.</p>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"79 ","pages":"100406"},"PeriodicalIF":2.2,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11338056/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141765706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preoperative evaluation profile of patients undergoing arterial vascular surgery in a tertiary hospital.","authors":"Arthur Souza Magnani, Leandro Teixeira de Castro, Isabela Cristina Kirnew Abud Manta, Viviane Galli Dib, Luiz Otávio Vittorelli, Felipe Soares Oliveira Portela, Nelson Wolosker, Marcelo Passos Teivelis","doi":"10.1016/j.clinsp.2024.100445","DOIUrl":"10.1016/j.clinsp.2024.100445","url":null,"abstract":"<p><strong>Background: </strong>Patients with peripheral arterial disease have an increased risk of developing cardiovascular complications in the postoperative period of arterial surgeries known as Major Adverse Cardiac Events (MACE), which includes acute myocardial infarction, heart failure, malignant arrhythmias, and stroke. The preoperative evaluation aims to reduce mortality and the risk of MACE. However, there is no standardized approach to performing them. The aim of this study was to compare the preoperative evaluation conducted by general practitioners with those performed by cardiologists.</p><p><strong>Methods: </strong>This is a retrospective analysis of medical records of patients who underwent elective arterial surgeries from January 2016 to December 2020 at a tertiary hospital in São Paulo, Brazil. The authors compared the preoperative evaluation of these patients according to the initial evaluator (general practitioners vs. cardiologists), assessing patients' clinical factors, mortality, postoperative MACE incidence, rate of requested non-invasive stratification tests, length of hospital stay, among others.</p><p><strong>Results: </strong>281 patients were evaluated: 169 assessed by cardiologists and 112 by general practitioners. Cardiologists requested more non-invasive stratification tests (40.8%) compared to general practitioners (9%) (p < 0.001), with no impact on mortality (8.8% versus 10.7%; p = 0.609) and postoperative MACE incidence (10.6% versus 6.2%; p = 0.209). The total length of hospital stay was longer in the cardiologist group (17.27 versus 11.79 days; p < 0.001).</p><p><strong>Conclusion: </strong>The increased request for exams didn't have a significant impact on mortality and postoperative MACE incidence, but prolonged the total length of hospital stay. Health managers should consider these findings and ensure appropriate utilization of human and financial resources.</p>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"79 ","pages":"100445"},"PeriodicalIF":2.2,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11338055/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141765707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Daytime bruxism, tardive orofacial dyskinesia, and dysphagia as side effects to duloxetine use over nine years in an octogenarian.","authors":"Carla A Scorza, Fulvio A Scorza, Josef Finsterer","doi":"10.1016/j.clinsp.2024.100438","DOIUrl":"10.1016/j.clinsp.2024.100438","url":null,"abstract":"","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"79 ","pages":"100438"},"PeriodicalIF":2.2,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11318533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141751309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NXP-2 positive dermatomyositis with marked dysphagia following an insect bite.","authors":"Larissa M Bombardi, Carla Alexandra Scorza, Josef Finsterer, Fulvio Alexandre Scorza","doi":"10.1016/j.clinsp.2024.100420","DOIUrl":"10.1016/j.clinsp.2024.100420","url":null,"abstract":"","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"79 ","pages":"100420"},"PeriodicalIF":2.2,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11295917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transient, self-limiting, antibody-negative myositis with venetoclax.","authors":"Carla A Scorza, Josef Finsterer, Fulvio A Scorza","doi":"10.1016/j.clinsp.2024.100444","DOIUrl":"10.1016/j.clinsp.2024.100444","url":null,"abstract":"","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"79 ","pages":"100444"},"PeriodicalIF":2.2,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11295995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin improves fertilization rate in assisted reproduction: Systematic review and meta-analysis.","authors":"Eduardo Carvalho de Arruda Veiga, Marise Samama, Fabio Ikeda, Giovanna Santos Cavalcanti, Amanda Sartor, Suelen Fernanda Parames, Edmund C Baracat, Joji Ueno, Jose Maria Soares Junior","doi":"10.1016/j.clinsp.2024.100397","DOIUrl":"10.1016/j.clinsp.2024.100397","url":null,"abstract":"<p><strong>Background: </strong>Melatonin is a hormone produced by the pineal gland and it has antioxidant properties.</p><p><strong>Aim: </strong>This study aimed to evaluate the effects of melatonin on assisted reproductive technologies through a systematic review and a meta-analysis.</p><p><strong>Materials and methods: </strong>Search strategies were used in PubMed and in other databases covering the last 15 years. After screening for eligibility, 17 articles were selected for the systematic review. For the meta-analysis statistics, two groups were formed, the treatment group (with melatonin) and the control group (without melatonin) for various assisted reproduction outcomes.</p><p><strong>Results: </strong>The main results were that no statistical differences were found concerning the clinical pregnancy outcome (p = 0.64), but there was a statistical difference with respect to Mature Oocytes (MII) (p = 0.001), antral follicle count (p = 0.0002), and the fertilization rate (p ≤ 0.0001).</p><p><strong>Conclusions: </strong>Melatonin had beneficial effects such as the improvement in the fertilization rate, although the authors did not obtain significance in the clinical pregnancy rate.</p>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"79 ","pages":"100397"},"PeriodicalIF":2.2,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11265587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141544618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}