Clinics最新文献

{"title":"Identification of prognostic biomarkers for endometrioid endometrial carcinoma based on the miRNA and mRNA co‐expression network regulated by estradiol","authors":"Qiu Xie ,&nbsp;Junting Huang ,&nbsp;Yuan Xie ,&nbsp;Jin Hu ,&nbsp;Li Jin","doi":"10.1016/j.clinsp.2025.100672","DOIUrl":"10.1016/j.clinsp.2025.100672","url":null,"abstract":"<div><h3>Background</h3><div>Endometrioid Endometrial Carcinoma (EEC), an estradiol-related disease, remains a serious health threat to women because of its high incidence and trend of rejuvenation. Accumulating evidence has highlighted that microRNAs (miRNAs) and messenger RNAs (mRNAs) play important roles in various biological processes involved in the pathogenesis of EEC. This study aimed to identify the potential prognostic biomarkers associated with EEC regulated by estradiol.</div></div><div><h3>Methods</h3><div>RNA expression profiles of EEC were obtained from The Cancer Genome Atlas database (<em>n</em> = 408) and the original sequencing, which was performed on endometrial cancer Ishikawa cells treated with 250 nM estradiol (<em>n</em> = 3), 50 nM estradiol (<em>n</em> = 3) or control (<em>n</em> = 3). The TargetScan database was used to predict the target genes of prognosis-related differentially expressed miRNAs (DEMs). Subsequently, functional enrichment analysis and topological analysis were performed on the overlaps of target genes and differentially expressed mRNAs (DEGs). Kaplan-Meier analysis was used to predict prognosis‐related target genes to identify prognostic biomarkers and cell population landscapes, and gene expression analysis was performed to locate prognosis-related DEGs based on single-cell transcriptomic sequencing data from the NCBI Sequence Read Archive database.</div></div><div><h3>Results</h3><div>Four estradiol-related DEGs were associated with prognosis, and 235 overlapping target DEGs were screened and incorporated into the functional enrichment analysis and protein-protein interaction network visualization studies. Additionally, SACS and GPR157 were identified as potential biomarkers for EEC prognosis through survival analyses. Furthermore, single-cell transcriptome data were analyzed to show changes in gene expression levels in specific cell types.</div></div><div><h3>Conclusions</h3><div>This study demonstrates that miR-142–5p–SACS and miR-30a-5p–GPR157, which are regulated by estradiol, may hold promise as diagnostic and prognostic biomarkers and novel therapeutic targets for EEC.</div></div>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"80 ","pages":"Article 100672"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Personalized nutrition and precision medicine in perimenopausal women: A minireview of genetic polymorphisms COMT, FUT2, and MTHFR","authors":"Pedro Andrade ,&nbsp;Aline Boveto Santamarina ,&nbsp;Jéssica Alves de Freitas ,&nbsp;Annete Bressan Rente Ferreira Marum ,&nbsp;Ana Flávia Marçal Pessoa","doi":"10.1016/j.clinsp.2024.100549","DOIUrl":"10.1016/j.clinsp.2024.100549","url":null,"abstract":"<div><div>This mini-review explores the potential of precision medicine and personalized nutrition in addressing health challenges faced by perimenopausal women, focusing on the role of genetic polymorphisms in key metabolic pathways. Specifically focus on the single nucleotide polymorphisms (SNPs) in the COMT, FUT2, and MTHFR genes, which influence neurotransmitter metabolism, gut microbiota composition, and folate homeostasis, respectively. These polymorphisms are critical in modulating hormonal fluctuations, metabolic imbalances, and nutrient absorption during perimenopause. The review highlights the impact of COMT rs4680 on stress response and mood disorders, FUT2 rs602662 and rs601338 on vitamin B12 absorption and cortisol metabolism, and MTHFR rs1801133 and rs1801131 on homocysteine levels and cardiovascular risk. Furthermore, the integration of machine learning in precision medicine is discussed, offering insights into how genetic data can optimize personalized interventions. This approach enables targeted nutritional and therapeutic strategies to mitigate the metabolic and psychological effects of perimenopause. Overall, this review underscores the importance of incorporating genetic testing into preventive care for perimenopausal women to enhance quality of life and promote healthy aging.</div></div>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"80 ","pages":"Article 100549"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of Licochalcone A on proliferation, invasion, and drug resistance of glioma cells by regulating TLR4/NF-κB signaling pathway","authors":"Baigui Zhou ,&nbsp;Kun Mu ,&nbsp;Xuzhou Yu ,&nbsp;Xu Chen ,&nbsp;Xiaoying Shi","doi":"10.1016/j.clinsp.2024.100542","DOIUrl":"10.1016/j.clinsp.2024.100542","url":null,"abstract":"<div><h3>Objective</h3><div>Based on Toll Like Receptor 4 (TLR4)/Nuclear Factor-κB (NF-κB) Exploring the effects of Licochalcone A (LCA) on the proliferation, invasion, and drug resistance of glioma cells through signaling pathways.</div></div><div><h3>Methods</h3><div>Cultivate human glioma cell line U251 in vitro, induce drug-resistant cell line U251/TMZ with Temozolomide (TMZ), and validate the results. Different concentrations of licorice chalcone A were used to treat U251 cells and U251/TMZ cells, and were named as control group, low-dose group, medium-dose group, and high-dose group, respectively. CCK-8 assay, cell adhesion assay, and Transwell assay were used to detect cell survival rate, cell adhesion rate, number of migrating cells, and number of invading cells, respectively.</div></div><div><h3>Results</h3><div>The cell survival rate, cell adhesion rate, number of migrating and invading cells in the high-dose group were lower than those in the medium-dose group and lower than those in the control group. High-dose group TLR4, NF-κB mRNA and protein levels were lower than those in the medium dose group and lower than those in the control group (<em>p</em> &lt; 0.05). Compared with the si-NC group, the si-TLR4 group showed a decrease in cell survival rate and adhesion rate, as well as a decrease in the number of migrating and invading cells, the levels of CyclinD1 and N-cadherin proteins decreased, while the levels of E-cadherin protein increased (<em>p</em> &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>LCA could inhibit the proliferation and metastasis of glioma cells and reverse drug resistance, possibly by inhibiting the TLR4/NF-κB signaling pathway.</div></div>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"80 ","pages":"Article 100542"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eugenol alleviates renal ischemia-reperfusion injury induced-endoplasmic reticulum stress via activating Sestrin2","authors":"Jingwei Liu,&nbsp;Xujie Sun,&nbsp;Junfeng Liang,&nbsp;Shiqiang Song","doi":"10.1016/j.clinsp.2025.100627","DOIUrl":"10.1016/j.clinsp.2025.100627","url":null,"abstract":"<div><h3>Introduction</h3><div>Renal Ischemia-Reperfusion Injury (RIRI) often arises due to heightened oxidative stress, rendering it a central focus of research. Sestrin2 plays a pivotal role in regulating oxidative stress; nevertheless, its impact on the renoprotective properties of Eugenol (EU) during RIRI warrants further investigation.</div></div><div><h3>Methods</h3><div>Mice and TCMK-1 cells were categorically assigned into six groups: Sham/Control, Ischemia-Reperfusion (IR)/HR (Hypoxia-Reoxygenation), IR/HR+EU, Sham/Control+Sestrin2-KO, IR/HR+Sestrin2-KO, and IR/HR+EU+Sestrin2-KO. The effects of EU and the involvement of Sestrin2 in RIRI/HR were evaluated using Urea Nitrogen (BUN), Creatinine (Scr), Superoxide Dismutase (SOD), Glutathione (GSH), Catalase (CAT), and Malondialdehyde (MDA) assay kits; western blotting; cell viability assays; HE-staining; and Reactive Oxygen Species (ROS) detection.</div></div><div><h3>Results</h3><div>Following RIRI/HR, a marked deterioration in kidney function and a significant surge in oxidative stress levels were observed. However, EU treatment ameliorated renal injury and inhibited oxidative stress. Additionally, EU upregulated Sestrin2 expression, and the renoprotective effects of EU were reversed upon Sestrin2 knockdown.</div></div><div><h3>Conclusion</h3><div>The present study posits that EU effectively mitigates RIRI/HRI (Hypoxia-Reoxygenation Injury), and its mechanism of renal protection potentially involves the upregulation of Sestrin2, coupled with the inhibition of oxidative and Endoplasmic Reticulum Stress (ERS).</div></div>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"80 ","pages":"Article 100627"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143704163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kynurenine promotes the immune escape of colorectal cancer cells via NAT10-mediated ac4C acetylation of PD-L1","authors":"Zaibiao Wang ,&nbsp;Manman Yin ,&nbsp;Ruhang Zhou ,&nbsp;Ming Li ,&nbsp;Jie Peng ,&nbsp;Zhengguang Wang","doi":"10.1016/j.clinsp.2025.100658","DOIUrl":"10.1016/j.clinsp.2025.100658","url":null,"abstract":"<div><h3>Background</h3><div>This study aimed to investigate the role of kynurenine in Colorectal Cancer (CRC) and the underlying mechanism.</div></div><div><h3>Methods</h3><div>Enzyme-linked immunosorbent assay was employed to assess the kynurenine concentration. Flow cytometry was utilized to analyze the percentages of CD3+CD4+ and CD3+CD8+ <em>T</em>-cells. Immunofluorescence was used to measure the expression of Programmed Death-Ligand 1 (PD-L1). RNA modification levels in CRC cells were analyzed using a dot blot assay. The interaction between NAT10 and PD-L1 was assessed via RNA immunoprecipitation, dual-luciferase reporter, and immunofluorescence assays. A xenograft tumor rat model was established.</div></div><div><h3>Results</h3><div>Results indicated that kynurenine suppressed T-cell activation and promoted immune escape. Besides, kynurenine promoted N-Acetyltransferase 10 (NAT10)-mediated N4-acetylcytidine (ac⁴C) modification. Moreover, NAT10 inhibition improved T-cell activation and suppressed immune escape. Mechanically, NAT10 is bound with the mRNA of PD-L1. Rescue experiments showed that PD-L1 inhibitor treatment reversed the suppressed T-cell activation and the promoted immune escape induced by NAT10 overexpression. In vivo, studies indicated that NAT10 deficiency reversed the promoted tumor growth induced by kynurenine treatment.</div></div><div><h3>Conclusion</h3><div>In conclusion, kynurenine promoted the immune escape of CRC cells via NAT10-mediated ac⁴C acetylation of PD-L1.</div></div>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"80 ","pages":"Article 100658"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TeleHCV: A single-visit protocol and minimal passive remote monitoring are sufficient to achieve high SVR with a sofosbuvir-velpatasvir regimen","authors":"Jerônimo De Conto Oliveira ,&nbsp;Fernando Comunello Schacher ,&nbsp;Marisa Boff Costa ,&nbsp;Maurício Godinho Kolling ,&nbsp;Raquel Boff Costa ,&nbsp;Henrique Cabral Scherer ,&nbsp;Paula Martins Fernandes ,&nbsp;Natan Katz ,&nbsp;Marcelo Rodrigues Gonçalves ,&nbsp;Dimitris Varvaki Rados ,&nbsp;Mário Reis Álvares-da-Silva","doi":"10.1016/j.clinsp.2025.100643","DOIUrl":"10.1016/j.clinsp.2025.100643","url":null,"abstract":"<div><h3>Aim</h3><div>Chronic Hepatitis-C Virus (HCV) treatment has evolved significantly in recent years with Direct-Acting Antivirals (DAAs). The traditional care cascade includes several steps that limit its impact, and simplification protocols have emerged. Therefore, the authors explore a simplified treatment strategy for HCV in a healthcare system with limited access to specialized care.</div></div><div><h3>Subject and methods</h3><div>Chronic HCV, DAA-naïve patients waiting for in-person specialized care were invited to a single-arm non-inferiority trial to evaluate a simplified treatment protocol with a single face-to-face appointment and minimal monitoring of antiviral therapy. The unique visit consisted of an HCV presentation followed by individual medical consultation, blood tests, and delivery of sofosbuvir-velpatasvir pills for a 12-week treatment. Patients were remotely monitored without scheduled on-treatment appointments or phone calls. After treatment, teleconsultation using video was offered. The primary outcome was Sustained Virological Response (SVR) 12-weeks post-treatment. It was analyzed with Intention-To-Treat (ITT) and Per-Protocol (PP) approaches. ClinicalTrials.gov: NCT04039698.</div></div><div><h3>Results</h3><div>The authors included 144 patients, of which 54.2 % were male, mean age was 52 years. Most individuals (84.7 %) had an APRI score &lt; 1. All patients received at least one dose of DAA, 139 completed antiviral therapy, and 131 had SVR evaluation. The ITT SVR rate was 90.3 % (130/144 patients; 95 % CI 84.2 %‒94.6 %), and the PP SVR was 99.2 % (130/131 patients; 95 % CI 95.8 %‒100 %). Eighty-three adverse events were reported, and 93 % were handled with remote care.</div></div><div><h3>Conclusion</h3><div>This simplified strategy achieved a high SVR rate in a population with restricted access to specialized care. Telehealth tools and minimal monitoring are promising components for policies aimed at HCV elimination.</div></div>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"80 ","pages":"Article 100643"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of the quarantine period due to the pandemic on the severity of Parkinson's disease and the quality of life of Parkinson's patients","authors":"Fatemeh Rafizadeh ,&nbsp;Masoud Saadat Fakhr ,&nbsp;Elham Esmaeili ,&nbsp;Zahra Amini ,&nbsp;Koosha Amiri ,&nbsp;Delyar Mardmomen ,&nbsp;Hadi Aqajani ,&nbsp;Rozita Hajibagher Naeeni","doi":"10.1016/j.clinsp.2025.100605","DOIUrl":"10.1016/j.clinsp.2025.100605","url":null,"abstract":"<div><h3>Objective</h3><div>The COVID-19 pandemic has a significant impact on the physical and mental health of people around the world. Parkinson's patients need to visit hospitals regularly to evaluate and adjust the dosage of drugs. Studies have shown that anxiety, exacerbated by existing conditions ‒ including the challenges posed by the COVID-19 pandemic ‒ can aggravate the symptoms of Parkinson's disease. This study aims to assess the impact of the quarantine period on the severity of movement symptoms, quality of life, non-motor symptoms, and the relationship with sex, education level, and age in patients with Parkinson's disease.</div></div><div><h3>Methods</h3><div>This descriptive cross-sectional study includes 200 patients with Parkinson's disease who were treated in Bu Ali Hospital from April 2019 to the end of 2021. Primary data was collected using the patients' files, which included information on age, gender, education level, medications and dosage, duration of Parkinson's disease, the severity of the disease was evaluated based on the Hohen and Yehr (H&amp;Y) criteria, and Quality of Life (QOL) and the impact of the quarantine period on their illness and quality of life has been collected through the Parkinson's Disease Questionnaire (PDQ-39) questionnaire. The collected data were analyzed using SPSS software, descriptive statistics, <em>t</em>-test, and analysis of variance.</div></div><div><h3>Results</h3><div>The quarantine period has a significant effect on the severity of Parkinson's disease and quality of life. The mean severity of the disease increased from 2.85 before quarantine to 3.30 during quarantine (<em>p</em> &lt; 0.05), indicating an increase in motor symptoms. Similarly, quality of life scores in all dimensions decreased from 62.8 before quarantine to 48.2 during quarantine (<em>p</em> &lt; 0.05), indicating a decrease in quality of life. Pearson's correlation test was used to investigate the relationship between age and the change in patients’ quality of life and the change in disease severity (<em>p</em> &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>The quarantine period due to the COVID-19 epidemic has had a significant impact on the severity of Parkinson's disease and the quality of life of patients. The findings of this study indicate the need to develop strategies to provide better health care, social support, and physical activity for patients with Parkinson's disease during quarantine and the pandemic.</div></div>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"80 ","pages":"Article 100605"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Malignant risk of thyroid nodules with isolated macrocalcifications – A study based on surgery results","authors":"Xi-Yue Yang ,&nbsp;Li-Fang Huang ,&nbsp;Yue-Jian Han ,&nbsp;Xiao-Xin Cen","doi":"10.1016/j.clinsp.2025.100657","DOIUrl":"10.1016/j.clinsp.2025.100657","url":null,"abstract":"<div><h3>Objective</h3><div>To determine the malignancy risk of thyroid nodules with Isolated Macrocalcifications (IMC) based on surgical results and evaluate the postoperative risk of malignant nodules with IMC.</div></div><div><h3>Methods</h3><div>A total of 46 thyroid nodules with IMC were enrolled from 3680 consecutive patients who underwent thyroidectomy between August 2018 and September 2023. The malignancy risk of IMC nodules, postoperative risk of malignant nodules, and whether the ultrasonic features of IMC (smooth, lobulated, or focal disruption of the anterior margin) were associated with malignancy were investigated. The nodules were further divided into three groups (group A, maximum diameter &lt; 10 mm; group B, maximum diameter of 10‒14 mm and group C, maximum diameter ≥ 15 mm). Differences in malignancy and Lymph Node Metastasis (LNM) risks were also evaluated among the three groups.</div></div><div><h3>Results</h3><div>The malignancy risk of the IMC nodules was 30.43% (14/46). Four patients developed LNM. Eight nodules were staged as T1aN0M0 and low-risk, whereas six nodules were staged as T1bN1aM0 and intermediate-risk. Focal disruption of the anterior margin of IMC was significantly associated with malignancy. Malignant and LNM risk showed no differences among nodules with different sizes.</div></div><div><h3>Conclusions</h3><div>IMC nodules with different sizes had a lower intermediate risk of malignancy and exhibited the same aggressive behavior. The cutoff value of these nodules for further Fine Needle Aspiration (FNA) warranted further investigation. Interruption of IMC was more often seen in malignant nodules, and more attention should be paid to these nodules.</div></div>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"80 ","pages":"Article 100657"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143869823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Olanzapine as a prophylactic antiemetic for preventing postoperative nausea and vomiting after general anesthesia: A systematic review and meta-analysis","authors":"Thiago Ramos Grigio ,&nbsp;Hans Timmerman ,&nbsp;Angela Maria Sousa ,&nbsp;André Paul Wolff","doi":"10.1016/j.clinsp.2025.100645","DOIUrl":"10.1016/j.clinsp.2025.100645","url":null,"abstract":"","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"80 ","pages":"Article 100645"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The educational impact of medical students’ participation in a short-term health expedition: The Iguape one health experience","authors":"Marcelo Arruda Candido ,&nbsp;Vinicius Venturini ,&nbsp;Matheus Polly ,&nbsp;Matheus Belloni Torsani ,&nbsp;Alexandre Sizilio ,&nbsp;João Mitsuji Sakô ,&nbsp;Wen-Jan Tuan ,&nbsp;Robert Patrick Lennon ,&nbsp;Anna Sara Shafferman Levin ,&nbsp;Iolanda de Fátima Lopes Calvo Tibério","doi":"10.1016/j.clinsp.2025.100601","DOIUrl":"10.1016/j.clinsp.2025.100601","url":null,"abstract":"<div><h3>Objectives</h3><div>The primary goal of this study is to evaluate the educational effects of medical students' participation in a health expedition project. Specific objectives include assessing students' perceptions of the educational value of the program, measuring gains in clinical knowledge and skills, evaluating behavioral changes, professional orientation, and students' willingness to work within the public health system (SUS) after graduation.</div></div><div><h3>Methods</h3><div>A quantitative methodology was employed, following Kirkpatrick's four-level evaluation model. Questionnaires were administered before and after participation in the expedition to assess students' reactions, learning, and empathy. The sample comprised all 15 students who participated in the expedition, with data analysis performed using R software.</div></div><div><h3>Results</h3><div>The analysis highlighted that the expedition experience significantly impacted student's career trajectories. Approximately 80 % of participants reported that the expedition positively influenced their career decisions, and 60 % expressed interest in working within the SUS. These findings suggest that while expeditions may not yield quantifiable gains in clinical competence in the short term, they can be catalysts in forming physicians oriented towards public service and social sensitivity.</div></div><div><h3>Conclusions</h3><div>Health expeditions like the one conducted may not offer significant improvements in measurable clinical competencies in the short term but have a substantial effect on students' career decisions, aligning with public health needs and reinforcing commitment to service in vulnerable contexts. This influence suggests that practical experiences in community settings play a crucial role in shaping medical students' professional aspirations, emphasizing the importance of such programs in medical education aimed at social responsibility and health equity.</div></div>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"80 ","pages":"Article 100601"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}