Hsa_circ_0000105 promotes nasopharyngeal carcinoma malignancy by miR-541-3p/S100A11 axis

IF 2.2 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

Clinics Pub Date : 2025-01-01 DOI:10.1016/j.clinsp.2025.100577

LuXing Peng , Jian Qin , DeFeng Qing , XinJun Huang , XiuRong Huang

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引用次数: 0

Abstract

Objective

This study was to investigate whether hsa_circ_0000105 is involved in the process of regulating Nasopharyngeal Carcinoma (NPC) biological behaviors and to reveal the molecular mechanism.

Methods

NPC tissues and normal tissues were collected, and NPC cell lines and normal control cell lines were obtained. hsa_circ_0000105/miR-541-3p/S100A11 was evaluated by RT-qPCR or Western blot. CCK-8 assay, EdU assay, Transwell assay, wound healing assay, flow cytometry, and Western blot were employed to evaluate the biological behaviors of NPC cells. Dual luciferase reporter assay and RIP assay were applied to evaluate the direct targeting relationship of hsa_circ_0000105/miR-541-3p/S100A11. A tumor xenotransplantation assay was implemented to evaluate the effect of hsa_circ_0000105 on NPC tumors.

Results

hsa_circ_0000105 and S100A11 were highly expressed in NPC, while miR-541-3p was lowly expressed. hsa_circ_0000105 competitively adsorbed miR-541-3p and mediated S100A11 expression. Silencing or upregulation of hsa_circ_0000105 restricts and induces malignant behavior in HNE2 cells, respectively. The impact of hsa_circ_0000105 silencing on malignant behaviors of HNE2 cells was blocked by miR-541-3p downregulation, while that of hsa_circ_0000105 upregulation was attenuated by S100A11 inhibition.

Conclusion

hsa_circ_0000105 acts as a carcinogenic factor in NPC and promotes NPC malignancy by miR-541-3p/S100A11 axis.

查看原文本刊更多论文

Hsa_circ_0000105通过miR-541-3p/S100A11轴促进鼻咽癌恶性。

目的：探讨hsa_circ_0000105是否参与鼻咽癌（NPC）生物学行为的调控过程，并揭示其分子机制。方法：收集鼻咽癌组织和正常组织，获得鼻咽癌细胞系和正常对照细胞系。RT-qPCR或Western blot检测hsa_circ_0000105/miR-541-3p/S100A11。采用CCK-8法、EdU法、Transwell法、创面愈合法、流式细胞术、Western blot法评价鼻咽癌细胞的生物学行为。采用双荧光素酶报告基因法和RIP法评估hsa_circ_0000105/miR-541-3p/S100A11的直接靶向关系。采用肿瘤异种移植试验评价hsa_circ_0000105对鼻咽癌肿瘤的影响。结果：hsa_circ_0000105和S100A11在NPC中高表达，miR-541-3p低表达。hsa_circ_0000105竞争性吸附miR-541-3p并介导S100A11表达。沉默或上调hsa_circ_0000105分别限制和诱导HNE2细胞的恶性行为。miR-541-3p下调可阻断hsa_circ_0000105沉默对HNE2细胞恶性行为的影响，而抑制S100A11可减弱hsa_circ_0000105上调的影响。结论：hsa_circ_0000105作为鼻咽癌的致癌因子，通过miR-541-3p/S100A11轴促进鼻咽癌恶性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinics 医学-医学：内科

CiteScore

4.10

自引率

3.70%

发文量

129

审稿时长

52 days

期刊介绍： CLINICS is an electronic journal that publishes peer-reviewed articles in continuous flow, of interest to clinicians and researchers in the medical sciences. CLINICS complies with the policies of funding agencies which request or require deposition of the published articles that they fund into publicly available databases. CLINICS supports the position of the International Committee of Medical Journal Editors (ICMJE) on trial registration.