TRIB3 is a biomarker of poor prognosis in laryngeal squamous cell carcinoma and may affect tumor development through PI3K / AKT / mTOR pathway

IF 2.2 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

Clinics Pub Date : 2025-01-01 DOI:10.1016/j.clinsp.2025.100576

Runsheng Yuan, Zhongqiang Cheng, Xiaodong Zhan

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引用次数: 0

Abstract

Objective

TRIB3 has been confirmed to participate in and regulate biological metabolic activities in head and neck tumors such as nasopharyngeal carcinoma and oropharyngeal carcinoma, so the purpose of this study was to explore whether there is a correlation between TRIB3 and Laryngeal Squamous Cell Carcinoma (LSCC) and to preliminarily explore the biological characteristics of TRIB3 in LSCC.

Methods

TRIB3 expression in the LSCC was analyzed based on The Cancer Genome Atlas (TCGA) database. CCK-8 assay, Colony Formation Assay, wound healing assay, and Transwell assay were performed to investigate the roles of TRIB3 in the proliferation, invasion and metastasis of LSCC. The Protein-Protein Interaction (PPI) network of TRIB3 was analyzed based on the STRING database. Western blotting and qRT-PCR were employed to detect the protein and mRNA expression of TRIB3.

Results

TRIB3 over-expressed in the LSCC, which was related to the poor prognosis of LSCC. Patients with methylation related to high TRIB3 expression had a poorer prognosis. Knock-down of TRIB3 expression suppresses the growth, invasion and migration of LSCCs via PI3K / AKT / mTOR. TIME analysis, surface checkpoint analysis, and prediction model indicated that TRIB3 related risk model displayed a poor prognosis.

Conclusion

This study investigates the role of TRIB3 in the biological characteristics of LSCC, and preliminarily concludes that TRIB3 may be a potentially promising prognostic biomarker for LSCC. TRIB3 may facilitate the growing, invasive and migrating LSCC, leading to a poor prognosis.

查看原文本刊更多论文

TRIB3是喉鳞癌预后不良的生物标志物，可能通过PI3K / AKT / mTOR通路影响肿瘤的发展。

目的：TRIB3已被证实参与和调节鼻咽癌、口咽癌等头颈部肿瘤的生物代谢活动，因此本研究的目的是探讨TRIB3与喉鳞癌（喉鳞癌）之间是否存在相关性，并初步探讨TRIB3在喉鳞癌中的生物学特性。方法：基于癌症基因组图谱（TCGA）数据库分析TRIB3在LSCC中的表达。采用CCK-8法、菌落形成法、创面愈合法和Transwell法研究TRIB3在LSCC增殖、侵袭和转移中的作用。基于STRING数据库对TRIB3蛋白-蛋白相互作用（PPI）网络进行分析。采用Western blotting和qRT-PCR检测TRIB3蛋白和mRNA的表达。结果：TRIB3在LSCC中过表达，与LSCC预后不良有关。与TRIB3高表达相关的甲基化患者预后较差。敲低TRIB3表达可通过PI3K / AKT / mTOR抑制LSCCs的生长、侵袭和迁移。时间分析、表面检查点分析和预测模型显示TRIB3相关风险模型预后较差。结论：本研究探讨了TRIB3在LSCC生物学特性中的作用，初步认为TRIB3可能是一种有潜力的LSCC预后生物标志物。TRIB3可能促进LSCC的生长、侵袭和迁移，导致预后不良。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinics 医学-医学：内科

CiteScore

4.10

自引率

3.70%

发文量

129

审稿时长

52 days

期刊介绍： CLINICS is an electronic journal that publishes peer-reviewed articles in continuous flow, of interest to clinicians and researchers in the medical sciences. CLINICS complies with the policies of funding agencies which request or require deposition of the published articles that they fund into publicly available databases. CLINICS supports the position of the International Committee of Medical Journal Editors (ICMJE) on trial registration.