Clinical, Cosmetic and Investigational Dermatology最新文献

{"title":"Beyond Lifting: Superficial-Targeted Monopolar Radiofrequency for Erythematotelangiectatic Rosacea.","authors":"Won-Serk Kim","doi":"10.2147/CCID.S639027","DOIUrl":"10.2147/CCID.S639027","url":null,"abstract":"<p><p>Noninvasive monopolar radiofrequency (NMRF) is an established modality for skin tightening, but its role in inflammatory dermatoses such as rosacea remains poorly defined. We report a man in his forties with an erythematotelangiectatic rosacea (ETR) phenotype, characterized by persistent centrofacial erythema, flushing, and xerosis, who underwent two sessions of multifrequency NMRF (XERF; Cynosure Lutronic, Goyang, Korea) at 4-week intervals using an off-label, superficial-targeted protocol (shallow setting, intensity level 5; 6.78 MHz; approximately 250 pulses; approximately 23,000 J cumulative energy per session; fragmented-pulse mode with controlled off-time). One month after the second session, facial erythema was visibly reduced and skin tone appeared more uniform. Antera 3D (Miravex, Dublin, Ireland) multispectral imaging showed a 24.3% decrease in average hemoglobin level (2.016 to 1.525) and a 36.9% decrease in hemoglobin variation (0.255 to 0.161). Surface scaling and xerosis also improved clinically. This single case suggests that superficial-targeted NMRF may reduce erythema and improve barrier-related symptoms in ETR through combined vascular, anti-inflammatory, and epidermal barrier mechanisms, beyond its established tissue-tightening effect, although these findings are exploratory and controlled studies incorporating standardized imaging, transepidermal water loss, histology, and inflammatory mediator assessment are needed to test this hypothesis.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"19 ","pages":"639027"},"PeriodicalIF":2.5,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13614337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149058876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Robustness Analyses Substantially Narrow in silico Network-Toxicology Signals Linking Selected Pharmaceuticals and Caffeine to Vitiligo.","authors":"Mahir Dığış, Kısmet Kaya, Betul Demir","doi":"10.2147/CCID.S639978","DOIUrl":"10.2147/CCID.S639978","url":null,"abstract":"<p><strong>Background: </strong>Network-toxicology and molecular-docking pipelines are widely used to link environmental chemicals to disease targets, but their outputs are rarely subjected to external validation, background-aware significance testing, or promiscuity controls.</p><p><strong>Methods: </strong>This fully computational (in silico) study applied a conventional network-toxicology and molecular-docking pipeline to vitiligo and eleven compounds-caffeine, four antibiotics, two angiotensin-receptor blockers, two statins, a proton-pump inhibitor and an antidepressant-adopted verbatim from a published study, holding chemical input constant. The output was stress-tested by core-tier threshold sensitivity, external cross-platform validation in two independent cohorts with the receiver-operating-characteristic (ROC) direction fixed in advance, background-restricted overlap-significance testing, and caffeine-exclusion sensitivity analysis.</p><p><strong>Results: </strong>The 102-gene target set did not intersect the high-confidence vitiligo core tier (overlap = 0, stable above 0.170) and gave a 22-gene extended-tier intersection, in which HIF1A was the highest-degree node and five key differentially expressed genes discriminated lesional from non-lesional skin at areas under the ROC curve (AUC) of 0.73-0.87. Every check degraded this. The overlap was enriched against a protein-coding background (fold 2.49, p = 5.1×10^-5) but not against the 5601-gene mineable universe (fold 1.29, p = 0.12). External discrimination did not replicate: with the direction fixed, seven of ten gene-cohort estimates fell below 0.50, none with a lower confidence bound above 0.50; HIF1A fell to 0.32 and 0.45, and EDNRA, apparently validating at 0.96 under automatic direction selection, reversed reproducibly to 0.04 (95% CI 0.00-0.24). Caffeine exclusion left two genes. No gene cleared all four checks.</p><p><strong>Conclusion: </strong>Validation and robustness checks converted an apparently supported mechanistic network into a result with no surviving gene-level candidate. The most consequential was a library default: within-cohort ROC direction selection turned a contradicted gene into the apparently strongest validation. Such checks should be standard, not optional. No causal or exposure-related association was demonstrated.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"19 ","pages":"639978"},"PeriodicalIF":2.5,"publicationDate":"2026-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13604017/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association and Mechanistic Insights Linking Bisphenol A to Psoriasis: Integrative Analysis of Bulk Transcriptomics, Network Toxicology, Mendelian Randomization and Single‑cell RNA Sequencing.","authors":"Lezhen Xu, Linxue Li, Wen Shi, Julin Gu","doi":"10.2147/CCID.S620116","DOIUrl":"10.2147/CCID.S620116","url":null,"abstract":"<p><strong>Purpose: </strong>Psoriasis is a chronic immune-mediated skin disease with complex etiology, and environmental factors such as bisphenol A (BPA) exposure are suspected pathogenic factors. This study aimed to identify potential diagnostic biomarkers linking BPA exposure to psoriasis and to explore the latent interaction and mechanisms between BPA and candidate biomarkers.</p><p><strong>Patients and methods: </strong>Psoriasis-related transcriptomic datasets (GSE14905, GSE13355, GSE183047) were downloaded from the Gene Expression Omnibus (GEO) database, and psoriasis-related genome-wide association study (GWAS) data were obtained from the GWAS Catalog. Target genes of psoriasis and BPA were retrieved from multiple public databases. A multi-omics integrative strategy was adopted in this study, combining bulk transcriptomics, network toxicology, Mendelian randomization, and single-cell RNA sequencing (scRNA-seq). Computational molecular binding prediction and in vivo animal experiments were further performed for biomarker validation.</p><p><strong>Results: </strong>PSMD1 and BECN1 were identified as potential biomarkers linking BPA exposure to psoriasis, showing significant upregulation in the psoriasis group and high diagnostic accuracy. Gene Set Enrichment Analysis (GSEA) revealed the involvement of these molecules in several key pathways, such as proteasome and oxidative phosphorylation. Immune infiltration analysis reported a positive correlation between these biomarkers and M1-type macrophage cell frequency, while stable binding capacity was predicted between biomarkers and BPA. scRNA-seq identified dendritic cells (DCs) as key immune cells, characterized by increased cell-cell communication in the psoriasis group. Moreover, pseudo-time trajectory analysis showed that PSMD1 exhibited elevated expression at the terminal stage of DC differentiation, whereas BECN1 displayed increased expression at both the early and terminal stages. In vivo experiments confirmed a dose-dependent effect of BPA exposure on the severity of psoriasis-like inflammation, followed by an increased expression of both PSMD1 and BECN1.</p><p><strong>Conclusion: </strong>This study identifies PSMD1 and BECN1 as potential diagnostic biomarkers associated with BPA exposure and psoriasis, offers predictive insights into their possible roles in related pathways and immune regulation, and sheds light on molecular-cellular mechanisms linking BPA exposure to psoriasis pathogenesis.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"19 ","pages":"620116"},"PeriodicalIF":2.5,"publicationDate":"2026-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A First-Principles Biomechanical Analysis of Graft Popping in Sharp Implanter Hair Transplantation.","authors":"Jae Hyun Park","doi":"10.2147/CCID.S634017","DOIUrl":"10.2147/CCID.S634017","url":null,"abstract":"<p><strong>Objective: </strong>To develop a first-principles biomechanical framework that may explain graft popping in sharp implanter hair transplantation and to apply it to two high-risk geometries-the anterior hairline and the parietal whorl.</p><p><strong>Methods: </strong>This theoretical analysis used simplified first-order geometric approximations of force-vector decomposition, bevel-tip geometry, scalp elasticity, and recipient-site pressure dynamics; no ex vivo or clinical measurements were performed. The model was applied to acute insertion angles and radial whorl implantation.</p><p><strong>Results: </strong>Bevel penetration is estimated to generate a lateral force of <i>F</i> _lateral = ½ sin(2<i>α</i>) · <i>F</i> _axial, approximately 20-25% of the axial force at bevel angles of 12-15°. As insertion angle decreases toward the hairline, the normalized in-plane push-out force index rises from approximately 25% at perpendicular insertion to 72% at 60° and 90-103% at 15°, depending on the sign of the bevel contribution. At the whorl, the angular divergence between adjacent follicular exit vectors follows Δφ = <i>d</i>/<i>r</i>, which would favor centrifugal implantation along the natural spiral progression. Curved finger-driven trajectories, oscillating rotation, and excessive tumescence may further increase lateral or ejection forces.</p><p><strong>Conclusion: </strong>Within this model, established preventive maneuvers for graft popping emerge as mechanical consequences of force direction, incision geometry, tissue elasticity, and recipient-site pressure coupling. The framework provides testable hypotheses for ex vivo, finite-element, and clinical validation rather than definitive clinical recommendations.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"19 ","pages":"634017"},"PeriodicalIF":2.5,"publicationDate":"2026-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete Skin Clearance is Associated with HADS-Defined Absence of Anxiety and Depressive Symptoms After IL-17A Inhibitor Treatment in Moderate-to-Severe Psoriasis: Evidence from the SPEECH Cohort.","authors":"Zhenquan Xuan, Yingbao Zhen, Didaer Muheyati, Dawei Huang, Lian Cui, Zeyu Chen, Min Dai, Yuxiong Jiang, Yangfeng Ding, Qian Yu, Yuling Shi","doi":"10.2147/CCID.S633054","DOIUrl":"https://doi.org/10.2147/CCID.S633054","url":null,"abstract":"<p><strong>Background: </strong>Psoriasis is a chronic immune-mediated disease associated with anxiety, depressive symptoms, and impaired quality of life, potentially involving interacting psychosocial and inflammatory pathways. During biologic treatment, psychological symptoms may improve alongside skin disease, but whether complete skin clearance (CSC) is associated with Hospital Anxiety and Depression Scale (HADS)-defined absence of anxiety and depressive symptoms remains unclear.</p><p><strong>Objective: </strong>To examine whether CSC, defined as PASI100, was associated with HADS-defined absence of anxiety and depressive symptoms after 12-week IL-17A inhibitor treatment in moderate-to-severe psoriasis.</p><p><strong>Methods: </strong>Patients receiving IL-17A inhibitors were identified from the Shanghai Psoriasis Effectiveness Evaluation CoHort (SPEECH). Eligible patients had complete baseline data, week-12 Psoriasis area and severity index (PASI) and HADS assessments. PASI responses represented percentage improvement from baseline and were categorized as PASI<75, PASI75-<100, or PASI100. The primary outcomes were week-12 HADS-A = 0 and HADS-D = 0, and the secondary outcomes were week-12 HADS-A and HADS-D as continuous outcomes. Logistic and linear regression, subgroup, and sensitivity analyses were performed.</p><p><strong>Results: </strong>Among 233 patients, 32 (13.7%) achieved PASI<75, 99 (42.5%) PASI75-<100, and 102 (43.8%) PASI100. Baseline HADS-A and HADS-D were comparable across groups. Compared with PASI100, PASI75-<100 was associated with lower odds of HADS-A = 0 (adjusted OR [aOR], 0.40; 95% confidence interval [CI], 0.20-0.78) and HADS-D = 0 (aOR, 0.48; 95% CI, 0.25-0.95), and higher week-12 HADS-A (adjusted β, 1.10; 95% CI, 0.34-1.86) and HADS-D (adjusted β, 0.95; 95% CI, 0.18-1.72). For PASI<75 versus PASI100, the corresponding aORs for HADS-A = 0 and HADS-D = 0 were 0.22 (95% CI, 0.08-0.62) and 0.27 (95% CI, 0.09-0.81), respectively. Subgroup and sensitivity analyses yielded consistent findings.</p><p><strong>Conclusion: </strong>CSC was associated with a higher likelihood of HADS-defined absence of anxiety and depressive symptoms in the treatment of moderate-to-severe psoriasis.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"19 ","pages":"633054"},"PeriodicalIF":2.5,"publicationDate":"2026-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Underperformance in Skin Cancer Practices Despite Good Knowledge and Attitudes Among Primary Care Physicians.","authors":"Abdulrahman Alqerafi, Naif Ahmed Alshehri, Khalid M Alghamdi","doi":"10.2147/CCID.S623206","DOIUrl":"10.2147/CCID.S623206","url":null,"abstract":"<p><strong>Background: </strong>Primary care physicians play an important role in the early recognition, prevention, and referral of suspicious skin lesions. However, data on their preparedness to address skin cancer in Saudi Arabia remain limited.</p><p><strong>Methods: </strong>In this nationwide cross-sectional study, primary care physicians completed an anonymous online questionnaire assessing knowledge, attitudes, practices, and perceived barriers. Knowledge was assessed using 27 items scored as correct or incorrect (range 0-27), with adequate knowledge defined a priori as ≥17/27 (≥60%). Attitudes were assessed using seven statements rated on a three-point agreement scale, scored 0-2 each (range 0-14), with positive attitudes defined as ≥10/14 (≥70%). Practice was summarised using a 0-5 index representing the number of recommended skin cancer-related behaviours performed, with proper practice defined as ≥3/5. Perceived barriers were assessed as a separate domain using the same three-point agreement format. Multivariable logistic regression was used to identify factors associated with proper practice.</p><p><strong>Results: </strong>A total of 159 primary care physicians were included. Mean knowledge and attitude scores were 20.9 ± 3.2 and 11.2 ± 1.9, respectively, and 90.6% and 89.3% met thresholds for adequate knowledge and positive attitudes. In contrast, the mean practice index was 2.0 ± 1.4, and only 34.6% met the definition of proper practice. Only 24.5% had performed a full-body skin examination for skin cancer screening, 23.3% routinely counseled patients on sun protection, 20.8% used diagnostic tools for suspicious lesions, and 32.7% had attended skin cancer-related continuing medical education. Training attendance and more years in practice were independently associated with proper practice. The most frequently reported barriers were lack of access to diagnostic tools (74.2%) and absence of clear clinical protocols for skin cancer screening or management (69.2%).</p><p><strong>Conclusion: </strong>Primary care physicians showed good knowledge and positive attitudes regarding skin cancer, but practice remained suboptimal. Improving training, clinical guidance, and access to diagnostic tools may strengthen skin cancer prevention and early detection in primary care.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"19 ","pages":"623206"},"PeriodicalIF":2.5,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13594507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149023945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coexistence of Erythrodermic Psoriasis and Bullous Pemphigoid: A Case Report.","authors":"Menglin Li, Tao Guo, Junling Zhang","doi":"10.2147/CCID.S630989","DOIUrl":"10.2147/CCID.S630989","url":null,"abstract":"<p><strong>Background: </strong>Erythrodermic psoriasis (EP) represents a severe inflammatory subtype of psoriasis. Bullous pemphigoid (BP), the most common autoimmune subepidermal blistering disorder in clinical practice, predominantly affects elderly populations. Comorbidity of these two diseases is uncommon, and BP secondary to EP in young-adult patients is particularly rare.</p><p><strong>Case presentation: </strong>A 33-year-old male with a 14-year relapsing-remitting history of psoriasis was admitted for erythrodermic psoriasis flare. Tense soybean-sized bullae with clear fluid appeared on bilateral lower extremities on the 5th hospital day. Skin biopsy demonstrated subepidermal blister formation with psoriasiform epidermal alterations. Direct immunofluorescence revealed basement-membrane-zone (BMZ) IgG (±) and C3 (+). Serum serology revealed markedly elevated levels of both anti-BP180 and anti-BP230 antibodies, whereas anti-desmoglein-1 and anti-desmoglein-3 antibodies were negative, supporting the diagnosis of coexisting EP and BP. The patient was treated with oral acitretin combined with high-potency topical halometasone cream, no new bullae occurred subsequently. Both psoriatic erythrodermic lesions and bullous lesions achieved marked improvement over the 6-month follow-up period.</p><p><strong>Conclusion: </strong>This rare case of BP secondary to EP in a young adult underscores that clinicians should maintain high suspicion for BP when tense bullae develop de novo in patients with EP. Timely performance of histopathology, perilesional direct immunofluorescence, and pemphigoid-targeted serological testing is critical for establishing a definitive diagnosis.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"19 ","pages":"630989"},"PeriodicalIF":2.5,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13592363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149016106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-Dose Corticosteroid Combined with Hydroxychloroquine for the Treatment of Generalized Annular Atrophic Lichen Planus: A Case Report.","authors":"Jinhui Xu, Li Chai","doi":"10.2147/CCID.S625316","DOIUrl":"10.2147/CCID.S625316","url":null,"abstract":"<p><strong>Purpose: </strong>Annular atrophic lichen planus (AALP) is a rare subtype of lichen planus. It is distinguished from other variants by the reduction of elastic fibers in the superficial dermis and the presence of annular skin lesions, which are characteristic features of this variant. AALP often exhibits a chronic course and poor response to treatment. The rare case presented here may provide valuable insights for dermatologists.</p><p><strong>Materials and methods: </strong>A case of a 57-year-old male diagnosed with generalized annular atrophic lichen planus is reported. Based on the clinical features and histopathological examination, a final diagnosis of generalized annular atrophic lichen planus was made. No significant abnormalities were found in the patient's baseline condition. Following the diagnosis of AALP, the patient was subjected to combination oral therapy with low-dose methylprednisolone (16 mg/day) and hydroxychloroquine (0.4 g/day).</p><p><strong>Results: </strong>A rapid resolution of the skin rash was observed two weeks after the initiation of treatment. During the clinical follow-up period of six months, no significant recurrence of the rash was noted.</p><p><strong>Conclusion: </strong>This case suggests that oral corticosteroids combined with hydroxychloroquine may be an effective treatment option for generalized annular atrophic lichen planus.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"19 ","pages":"625316"},"PeriodicalIF":2.5,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13594522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149023964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cxcl9 as a Marker of Adipose Tissue Changes Caused by Bariatric Surgery and Psoriasis.","authors":"Zheng Zhou, Ling Kuang","doi":"10.2147/CCID.S640352","DOIUrl":"10.2147/CCID.S640352","url":null,"abstract":"<p><strong>Background: </strong>Bariatric surgery has been shown to improve psoriasis, but the underlying molecular mechanisms remain unclear. This study aimed to investigate shared molecular mechanisms between bariatric surgery-induced adipose changes and psoriasis using bioinformatics approaches.</p><p><strong>Methods: </strong>Transcriptomic data from adipose tissue before and after bariatric surgery and from psoriatic lesions were obtained from the GEO database. Common differentially expressed genes (co-DEGs) were identified and subjected to GO/KEGG enrichment analysis, protein-protein interaction network construction, and machine learning-based hub gene selection. Diagnostic performance and immune infiltration characteristics were evaluated.</p><p><strong>Results: </strong>Co-DEGs were significantly enriched in the IL-17 signaling pathway, defense response to bacterium, response to lipopolysaccharide, cytolysis, and collagen-containing extracellular matrix. CXCL9 was identified as a key hub gene via multiple machine learning algorithms, showing good diagnostic performance in external validation (AUC > 0.85). Immune infiltration analysis revealed that CXCL9 expression was positively correlated with M1 macrophage and CD8⁺ T cell abundance, and negatively correlated with M2 macrophages.</p><p><strong>Conclusion: </strong>CXCL9, through its involvement in the IL-17 signaling pathway and lipopolysaccharide response, may serve as a critical bridge linking adipose tissue inflammation and psoriatic skin immunity, providing a novel molecular target for the improvement of psoriasis following bariatric surgery.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"19 ","pages":"640352"},"PeriodicalIF":2.5,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13594523/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149023910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative Transcriptomic and Machine Learning Analysis Identifies JAG1 and NR2F2 in Keloid.","authors":"Lingyi Yang, Wenshen Mo, Wendi Cao, Lin Mi, Xiaowei Wu","doi":"10.2147/CCID.S625100","DOIUrl":"10.2147/CCID.S625100","url":null,"abstract":"<p><strong>Objective: </strong>To identify key genes associated with keloid through integrative analysis of multiple transcriptomic datasets, and to explore their potential regulatory roles in disease development with experimental validation.</p><p><strong>Methods: </strong>Transcriptomic data of keloid were retrieved from the Gene Expression Omnibus (GEO) database. Batch effects were corrected using SVA and ComBat. Differentially expressed genes (DEGs) were identified using the <i>limma</i> package. Weighted gene co-expression network analysis (WGCNA) was performed to detect key modules, and intersected with DEGs to obtain candidate genes. Multiple machine learning algorithms were applied to identify key feature genes, which were further validated in independent external datasets. In parallel, wound tissues from a silicone-splinted murine excisional wound model (which recapitulates mechanical tension of human skin injury) and matched normal skin tissues were collected for histological assessment using haematoxylin-eosin (H&E) and Masson staining, and gene expression levels were validated by quantitative real-time PCR (qRT-PCR).</p><p><strong>Results: </strong>A total of 82 DEGs were identified. WGCNA revealed the MEpink module as the key module, and intersection analysis yielded 61 candidate genes, which were primarily enriched in inflammation- and fibrosis-related pathways. Protein-protein interaction network analysis indicated that JAG1 and NR2F2 occupied central positions within the network. Machine learning identified six key feature genes, among which NR2F2 and IL15 showed relatively strong discriminatory performance. Histological analysis demonstrated increased collagen deposition and disorganized tissue architecture in keloid samples. qRT-PCR confirmed that JAG1 and NR2F2 were upregulated in keloid tissues, consistent with transcriptomic findings.</p><p><strong>Conclusion: </strong>JAG1 and NR2F2 participate in inflammation- and fibrosis-related signaling cascades to drive keloid pathogenesis, and possess promising value for the molecular subtyping of keloid lesions. Collectively, our results lay a solid foundation for future mechanistic research on the molecular regulatory networks governing keloid development.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"19 ","pages":"625100"},"PeriodicalIF":2.5,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13592341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149016103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}