Clinical, Cosmetic and Investigational Dermatology最新文献

{"title":"Pseudoxanthoma Elasticum-Like Papillary Dermal Elastolysis (PXE-PDE) in an Elderly Female: A Rare Diagnostic Entity.","authors":"Naif Alshehri, Reem Alsergani, Abdulrahman Alqerafi, Sadan Mohammed Al Harbi, Ahmed Alhumidi, Mohammed A Alfada","doi":"10.2147/CCID.S548675","DOIUrl":"https://doi.org/10.2147/CCID.S548675","url":null,"abstract":"<p><p>Pseudoxanthoma elasticum-like papillary dermal elastolysis (PXE-PDE) is a rare, acquired skin disorder characterized by multiple small papules arising from degeneration of elastic fibers in the dermis. It is often underrecognized due to its benign nature and resemblance to harmless age-related skin changes and may be misdiagnosed as conditions such as pseudoxanthoma elasticum (PXE). A 70-year-old woman presented with asymptomatic skin lesions on her neck, which had gradually increased over 2-3 years. On physical examination, multiple confluent yellowish-white papules with a cobblestone appearance were observed on the neck. Skin biopsy confirmed the diagnosis of PXE-PDE, showing loss of elastic tissue in the superficial dermis. The patient underwent two sessions of CO₂ laser treatment, with approximately 40% improvement noted by both the physician and the patient. This case underscores the importance of clinicopathologic correlation in diagnosing PXE-PDE and distinguishing it from PXE and other mimickers. CO₂ laser therapy may be considered as a cosmetic treatment option in selected patients.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"18 ","pages":"2451-2455"},"PeriodicalIF":2.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Genetically Elevated Omega-3 Polyunsaturated Fatty Acids and Skin Disease Risk: A Mendelian Randomization Study.","authors":"Jia Min Chen, Yan Wang, Yan Shi","doi":"10.2147/CCID.S524519","DOIUrl":"https://doi.org/10.2147/CCID.S524519","url":null,"abstract":"<p><strong>Background: </strong>Omega-3 polyunsaturated fatty acids (PUFAs) are potential targets for the treatment of skin diseases due to their anti-inflammatory and immunomodulatory effects. By leveraging a genetic approach known as Mendelian randomization (MR), we sought to determine the causal impact of PUFAs on the likelihood of developing skin diseases among individuals of European ancestry.</p><p><strong>Methods: </strong>We integrated GWAS data from the CHARGE consortium and UK Biobank to identify genetic instruments for omega-3 PUFAs and desaturase activity, using two-sample MR to assess their associations with six skin diseases.</p><p><strong>Results: </strong>Elevated levels of omega-3 fatty acids were found to substantially lower the probability of experiencing atopic dermatitis (0.92, [0.85,0.98]), while increased DPA levels correlated with a substantial increase in the probability of squamous cell carcinoma occurrence (2.25, [1.29,3.92]). Increased DHA levels were also associated with a reduced risk of atopic dermatitis (0.90, [0.84,0.96]) but increased the risk of solar dermatitis (1.38, [1.09,1.73]). In addition, tissue-type specific MR analysis revealed that elevated FADS1 expression in fibroblasts significantly inhibited atopic dermatitis development (β = -0.181, [-0.276,-0.0853]), while elevated FADS2 expression in non-sun-exposed skin tissues was associated with a reduced risk of squamous cell carcinoma (β = -0.562, [-0.833,-0.029]). Conversely, heightened FADS2 expression was strongly linked to a greater likelihood of developing atopic dermatitis in both sun-exposed and sun-protected skin areas (β = 0.107, [0.0348,0.179]; β = 0.192, [0.114,0.0270], respectively).</p><p><strong>Conclusion: </strong> This study reveals the causal role of omega-3 PUFAs and FADS expression in specific tissues and blood in skin diseases. These findings underscore the potential of PUFA biosynthesis pathways as therapeutic targets for skin disease interventions.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"18 ","pages":"2463-2474"},"PeriodicalIF":2.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12484113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety and Clinical Efficacy of Kūlaris^TM, an Herbal Supplement for Mild to Severe Acne Vulgaris - A 12-Week Randomized, Double Blind, Placebo-Controlled Study.","authors":"Heggar Venkataramana Sudeep, Divya Chandradhara, Kodimule Shyamprasad","doi":"10.2147/CCID.S539004","DOIUrl":"https://doi.org/10.2147/CCID.S539004","url":null,"abstract":"<p><strong>Purpose: </strong>There is a growing interest in nutraceutical supplements for ameliorating skin disorders owing to their multifaceted role and tolerability. In this study, we investigated the safety and efficacy of a unique herbal formulation (Kūlaris^TM) containing <i>Commiphora wightii</i> (guggul) and <i>Amorphophallus konjac</i> tuber extracts for the treatment of mild-to-severe acne.</p><p><strong>Patients and methods: </strong>In a randomized, placebo-controlled, parallel-group study design, 100 male and female subjects (18-40 years) with mild to severe acne were allocated (1:1 ratio) to receive 450 mg capsules of either Kūlaris or placebo once daily for 12 weeks.</p><p><strong>Results: </strong>Kūlaris treatment markedly reduced acne severity, with the majority of subjects achieving success in the Investigator Global Assessment (IGA) score (odds ratio = 7.88, <i>p</i><0.0001) compared with placebo. Kūlaris administration significantly reduced the inflammatory and non-inflammatory acne lesion counts (<i>p</i><0.0001 vs placebo). The Cardiff Acne Disability Index (CADI) score was reduced significantly in the Kūlaris group after 6 (<i>p</i><0.0001) and 12 weeks (<i>p</i><0.001) from baseline, indicating an improvement in quality of life. However, the subjective assessment showed no significant changes in general skin health measures, such as skin quality, color, wrinkle reduction, and skin hydration, in either group. Kūlaris was well tolerated among study participants, with no reported adverse effects or observable clinical signs throughout the intervention period.</p><p><strong>Conclusion: </strong>This study provides compelling evidence to support the potential use of Kūlaris as a therapeutic supplement or an adjunctive therapy to treatment of acne vulgaris.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"18 ","pages":"2475-2488"},"PeriodicalIF":2.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12484112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Validation of Hub Genes in Hidradenitis Suppurativa.","authors":"Yi Ning Zhai, Heng Yue Cheng, Jun Ma, Cheng Cheng Feng, Qian Zhang, Wen Bo Bu, Wen Jin Miao, Hui Shen, Chen Ji","doi":"10.2147/CCID.S537660","DOIUrl":"10.2147/CCID.S537660","url":null,"abstract":"<p><strong>Background: </strong>Hidradenitis suppurativa (HS) is a severe, chronic inflammatory disease characterized by inflammatory nodules, progressive sinus tracts, and fistulas. Currently, its pathogenesis remain incompletely understood, and while diverse treatments are available, these have suboptimal efficacy. Herein, we analyzed the relevant genes and pathways in HS using bioinformatics to provide directions for the development of novel treatment.</p><p><strong>Methods: </strong>Two HS datasets were obtained, and differentially expressed genes associated with HS were identified. Enrichment analysis was performed, and a protein-protein interaction network of differential genes was produced. Genes were analyzed using the CytoHubba plugin to obtain the hub genes. For validation, we analyzed the differentially expressed genes between the affected skin tissues of patients with HS and normal human skin tissues.</p><p><strong>Results: </strong>Overall, 180 differential genes associated with HS were identified. Differentially expressed genes were mainly enriched in leukocyte migration, serine hydrolase activity, and serine-type peptidase activity. In total, 69 transcription factors, 115 microRNAs, and 41 drugs associated with hub genes were identified. The expression of <i>FCGR2A</i> and <i>IL2RG</i> was significantly upregulated in the HS disease group compared with that in the normal group.</p><p><strong>Conclusion: </strong>Ten hub genes were identified. Among these, <i>FCGR2A</i> is responsible for the phagocytosis and clearance of immune complexes, the dysregulation of which may represent an important link in the pathogenesis of HS. The PI3K-Akt-mTOR signaling pathway, regulated by the <i>IL2RG</i> gene, showed a close relationship with HS severity and the mechanism of scarring, presenting a potential drug target. The miR-3689 targeting the gene FCGR2A might also be potential biomarkers.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"18 ","pages":"2403-2422"},"PeriodicalIF":2.2,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12478222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skin Eczema Limited to Transplanted Hand.","authors":"Piotr Nockowski, Iwona Chlebicka, Aleksandra Stefaniak, Magdalena Krajewska, Dorota Kaminska, Jacek C Szepietowski","doi":"10.2147/CCID.S549774","DOIUrl":"https://doi.org/10.2147/CCID.S549774","url":null,"abstract":"<p><p>Hand transplantation is a vascularized type of composite tissue allotransplantation (CTA), involving the transfer of heterogeneous tissues such as skin, fat, bones, muscles, and nerves. Even with modern immunosuppression protocols, rejection remains a major obstacle in this field. We report the first documented case of eczema limited to the skin of a transplanted hand in a 38-year-old male born without a left hand. The patient underwent hand allotransplantation in December 2016 from a deceased donor with complete HLA mismatch and remained on stable triple immunosuppressive therapy. Six years post-transplantation, erythematous macules, papules, scaling, and increased keratosis appeared exclusively on the dorsal and palmar aspects of the grafted hand, involving approximately 80% of its skin surface, without lesions elsewhere. Histopathological examination confirmed subacute eczema, with no signs of acute or chronic rejection. Topical mometasone furoate ointment combined with an increased dose of oral prednisone (from 5 mg/day to 10 mg/day) resulted in marked improvement within two weeks, followed by complete resolution after several weeks of regular emollient use. No recurrence was observed during six months of follow-up. This case highlights the importance of differentiating between rejection and other dermatological conditions, such as eczema, in limb transplant recipients. Because eczema can closely mimic rejection, it may complicate post-transplant care and lead to unnecessary changes in immunosuppressive therapy if misdiagnosed. Accurate diagnosis is essential for appropriate management and graft survival.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"18 ","pages":"2457-2462"},"PeriodicalIF":2.2,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12479371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Sedentary Lifestyle, Physical Activity, and Skin Cancer Among Outpatient US Adults: Evidence from NHANES.","authors":"Ze-Qun Qiu, Shan-Yuan Ye, Run-Dong Zhang, Meng Cao, Yan Wang","doi":"10.2147/CCID.S545546","DOIUrl":"10.2147/CCID.S545546","url":null,"abstract":"<p><strong>Background: </strong>Sedentary behavior has been identified as a potential risk factor for various malignancies, whereas physical activity serves as a protective factor for certain cancers. The aim of this study was to explore the associations between these two factors and the prevalence of melanoma and non-melanoma skin cancers (NMSCs).</p><p><strong>Methods: </strong>We collected cross-sectional data from 26,731 participants in the NHANES from 2007 to 2016. Multivariate logistic regression and subgroup analyses were used to assess the correlations between sedentary behavior, physical activity, and the prevalence of melanoma and NMSCs.</p><p><strong>Results: </strong>Multivariable logistic regression indicated that sedentary behavior was not significantly associated with melanoma or NMSCs. The type and score of physical activity were not related to melanoma (p > 0.05). However, moderate-intensity physical activity (including both work and recreation activities) and physical activity scores in the 2001-4000 range were positively correlated with NMSCs. Subgroup analysis and interaction tests showed no significant differences in NMSC prevalence between participants with a physical activity score ranging from 2001-4000, suggesting that gender, Poverty Income Ratio (PIR), and education level were not significantly related to this positive correlation (p < 0.05). However, after adjusting for UV radiation as a covariate, the logistic regression results of the NMSCs were no longer significant.</p><p><strong>Conclusion: </strong>Our study results indicate that, for individuals aged 20 and above in the US, SB is not associated with the prevalence of melanoma or NMSCs. There is no significant correlation between physical activity and the prevalence of melanoma, but a positive correlation exists with NMSCs'. However, this association was attenuated and lost statistical significance after adjustment for sunburn history as a proxy for UV exposure. We need more prospective and well-designed studies to validate our findings to promote early prevention and lifestyle interventions for high-risk populations of NMSCs.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"18 ","pages":"2439-2450"},"PeriodicalIF":2.2,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Single-Cell Triaptosis Regulatory Pattern in the Immune Microenvironment of Keloids.","authors":"Jiaheng Xie, Songyun Zhao, Dan Wu, Yeqi Feng, Chenfeng Ma, Wei Yan, Ming Wang","doi":"10.2147/CCID.S536776","DOIUrl":"10.2147/CCID.S536776","url":null,"abstract":"<p><strong>Introduction: </strong>Keloids are a complex pathological condition of the skin characterized by abnormal proliferation of fibrous tissue and excessive accumulation of extracellular matrix, typically following inflammation after skin injury. Understanding the regulatory mechanisms of immune cells involved in keloid formation is essential for the development of effective treatments.</p><p><strong>Methods: </strong>This study integrated publicly available single-cell RNA sequencing (scRNA-seq) data with our own keloid scRNA-seq samples to investigate the role of triaptosis in shaping the immune microenvironment of keloids. We analyzed the composition and functional status of fibroblast and immune cell subpopulations.</p><p><strong>Results: </strong>Immune cells in keloids, especially CD8⁺ T cells and macrophages, showed significant heterogeneity under the influence of triaptosis regulatory patterns. These triaptosis-associated immune cell clusters exhibited distinct signaling interference compared to mesenchymal fibroblasts and contributed to keloid development. Furthermore, ELMO2 was identified as a key gene with a potential causal relationship to keloids using Summary-data-based Mendelian Randomization and validated through immunofluorescence staining.</p><p><strong>Conclusion: </strong>Our findings reveal the complexity of cell-cell interactions in the keloid immune microenvironment and highlight triaptosis as a potential regulatory mechanism in keloid pathogenesis. The identification of ELMO2 as a key factor offers a promising therapeutic target. This study lays a foundation for developing novel therapeutic strategies and encourages future investigations into the clinical application of triaptosis-related interventions for keloid treatment.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"18 ","pages":"2423-2437"},"PeriodicalIF":2.2,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145190918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Secondary Perianal Paget's Disease Originating From Adenocarcinoma of the Anorectal Junction: A Case Report.","authors":"Jun He, Rong-Chao He, Jian-Zhong Peng, Hou-Dong Wang, Tao Wang, Si-Jia Lu, Xiu-Feng Zhang, Zhong Shen","doi":"10.2147/CCID.S546040","DOIUrl":"10.2147/CCID.S546040","url":null,"abstract":"<p><p>Secondary extramammary Paget's disease (EMPD), originating from the cutaneous dissemination of malignant cells, represents a rare subtype prone to misdiagnosis. This study presents a case of secondary perianal Paget's disease (PPD) associated with adenocarcinoma at the anorectal junction. Preoperative full-thickness mapping biopsy was utilized for the assessment of lesion margins. The patient demonstrated favorable postoperative recovery following a multimodal therapeutic regimen.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"18 ","pages":"2395-2401"},"PeriodicalIF":2.2,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12459376/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145147941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guselkumab In A Subset Of Multifailure Patients With Hidradenitis Suppurativa: Real-Word Evidence from Federico II.","authors":"Fabrizio Martora, Nello Tommasino, Luigi Coronella, Luca Potestio, Teresa Battista, Matteo Megna","doi":"10.2147/CCID.S544180","DOIUrl":"10.2147/CCID.S544180","url":null,"abstract":"<p><strong>Introduction: </strong>Hidradenitis suppurativa (HS) is a chronic, debilitating inflammatory skin disorder characterized by painful nodules and abscesses, leading to substantial impairment in quality of life. Treatment remains challenging, particularly for patients with prior treatment failure or contraindications to anti-TNFα and anti-IL-17 therapies.</p><p><strong>Methods: </strong>This prospective, real-life study assessed the efficacy and safety of guselkumab, an anti-IL-23 monoclonal antibody, in 10 adult patients with moderate-to-severe HS who had previously failed or were contraindicated for anti-TNFα and/or anti-IL-17 therapy. Guselkumab was administered using the psoriasis-approved dosing regimen. Patients were evaluated at baseline (week 0), week 16, week 32 and week 48 using the International Hidradenitis Suppurativa Severity Score System (IHS4), Dermatology Life Quality Index (DLQI), and visual analog scale (VAS) for pain. The primary endpoint was achievement of Hidradenitis Suppurativa Clinical Response (HiSCR) at week 16.</p><p><strong>Results: </strong>At week 16, 40% of patients achieved HiSCR, increasing to 50% by week 48. Mean IHS4 scores decreased from 14.3 at baseline to 9.3 at week 16. This was accompanied by an average 10-point reduction in DLQI and a 4-point reduction in VAS pain scores. Despite these improvements, overall quality of life remained moderately impaired. One patient discontinued guselkumab due to loss of efficacy.</p><p><strong>Discussion: </strong>The results support previous findings from smaller studies and case reports, indicating that guselkumab may offer clinical benefit in select HS populations, particularly those with comorbid psoriasis or Crohn's disease. Although limited by a small sample size, this study contributes to the growing real-world evidence supporting guselkumab as a potential treatment option in multi-refractory HS. Larger, placebo-controlled trials are needed to further define its role in treatment algorithms.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"18 ","pages":"2387-2393"},"PeriodicalIF":2.2,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12456752/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145136601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary Histological Evidence of Epidermal and DEJ Remodeling with Microneedling-Assisted Topical Exosome Therapy: A Single-Subject Case Report.","authors":"Young Seob Lee","doi":"10.2147/CCID.S542022","DOIUrl":"10.2147/CCID.S542022","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to evaluate the histological effects of microneedling-assisted topical exosome therapy on the dermoepidermal junction (DEJ) and surrounding epidermal structures in aging skin.</p><p><strong>Patients and methods: </strong>In this single-subject case report, a healthy 63-year-old male with no known dermatologic or systemic conditions underwent a single session of 0.3 mm microneedling immediately followed by topical application of an exosome-based formulation (E-50 Skin Booster, PrimaCure Co., Ltd., Korea). A 3 mm punch biopsy was collected from the postauricular area at baseline, and a follow-up biopsy was obtained eight weeks post-treatment from an adjacent site. Hematoxylin and eosin (H&E) staining was performed, and histomorphometric parameters were analyzed using high-resolution imaging and AI-assisted quantitative tools.</p><p><strong>Results: </strong>Histological analysis revealed significant remodeling of the DEJ, with a 17.08% increase in rete ridge count (<i>p</i> = 0.02) and a 7.75% increase in ridge amplitude (<i>p</i> = 0.04). Mean epidermal thickness increased by 52.33% (<i>p</i> = 0.01), and basal keratinocyte density rose by 60.90% (<i>p</i> = 0.01), indicating enhanced regenerative activity. Collagen fiber density in the upper dermis improved by 5.77% (<i>p</i> = 0.05). No adverse effects were reported, and post-treatment tissue showed no signs of inflammation or fibrosis.</p><p><strong>Conclusion: </strong>Microneedling-assisted topical exosome therapy produced notable structural improvements at the epidermal and dermoepidermal levels in aging skin. Restoration of rete ridges, enhanced keratinocyte proliferation, and increased collagen density suggest a promising role for this combined approach in skin rejuvenation. These findings are specific to microneedling-assisted topical delivery and should not be interpreted as supporting the use of injectable exosomes, which were not evaluated in this study.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"18 ","pages":"2377-2385"},"PeriodicalIF":2.2,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145130201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}