International Journal of Advances in Medical Biotechnology - IJAMB最新文献_第2页

Bacterial cellulose/calcium alginate hydrogels in wound’s cicatricial process of diabetic foot implementation: case report 细菌纤维素/海藻酸钙水凝胶在糖尿病足创面瘢痕过程中的应用:1例报告

International Journal of Advances in Medical Biotechnology - IJAMB Pub Date : 2021-10-01 DOI: 10.52466/ijamb.v4i2.100

Gabriela Arruda Da Silva, E. M. Zanchin, Andressa Mendes Da Silva, Matheus Bertanha, Antonio Eduardo de Aquino Junior, F. Carbinatto, H. G. de Oliveira Barud, H. Barud

引用次数: 0

Innovation projects at the University-Pharmaceutical Industry interface: challenges and opportunities 大学-制药行业界面的创新项目:挑战与机遇

International Journal of Advances in Medical Biotechnology - IJAMB Pub Date : 2021-09-01 DOI: 10.52466/ijamb.v4i2.94

Leonardo Miziara Barboza Ferreira, Lincoln Miziara Barboza Ferreira, Jamily Angela Sant Anna Carvalho, Paulo Yun Cha, Ana Beatriz Figueiredo Oliveira

{"title":"Innovation projects at the University-Pharmaceutical Industry interface: challenges and opportunities","authors":"Leonardo Miziara Barboza Ferreira, Lincoln Miziara Barboza Ferreira, Jamily Angela Sant Anna Carvalho, Paulo Yun Cha, Ana Beatriz Figueiredo Oliveira","doi":"10.52466/ijamb.v4i2.94","DOIUrl":"https://doi.org/10.52466/ijamb.v4i2.94","url":null,"abstract":"COVID-19 pandemic accelerated the digital transformation of pharmaceutical R&D. This paper aimed to diagnose some key aspects of University-Pharmaceutical Industry (U-Pharma) partnerships in Brazil that should be addressed in digital platforms to enable the creation of innovative solutions to COVID-19. A qualitative research was applied through an online guiding questionnaire to identify the main points that are considered as opportunities or challenges by the parties involved in the University-Pharmaceutical Industry interface. The responses were processed using content analysis to raise common themes. Further, in-depth interviews were performed to evaluate the subjective perception of these themes. The results of the questionnaire showed the topics bureaucracy, partnership, and agility as the most relevant. The in-depth interviews showed the subjective perception of these themes. The joint analysis of the results showed that in addition to problems related to project management processes, issues that involve the human dimension, such as pro-executive behavior and communication skills, are also extremely relevant to the success of U-Pharma projects. The development of digital solutions for Knowledge Management of innovative projects of U-Pharma collaborations should take into consideration both the project and human dimensions. It is recommended that Project and People Management should be integrated into digital platforms.","PeriodicalId":103125,"journal":{"name":"International Journal of Advances in Medical Biotechnology - IJAMB","volume":"2010 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114792949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous alterations in ovaries and bone as a result of Polycystic Ovary Syndrome 多囊卵巢综合征引起的卵巢和骨骼的同时改变

International Journal of Advances in Medical Biotechnology - IJAMB Pub Date : 2021-03-18 DOI: 10.25061/IJAMB.V3I2.81

A. Bonfá, E. D. Alves, V. Fabrício, K. Nonaka, J. Anselmo-Franci, J. Achcar, L. Montrezor

{"title":"Simultaneous alterations in ovaries and bone as a result of Polycystic Ovary Syndrome","authors":"A. Bonfá, E. D. Alves, V. Fabrício, K. Nonaka, J. Anselmo-Franci, J. Achcar, L. Montrezor","doi":"10.25061/IJAMB.V3I2.81","DOIUrl":"https://doi.org/10.25061/IJAMB.V3I2.81","url":null,"abstract":"Polycystic ovary syndrome (PCOS) is one of the most widely recognized endocrine disorders affecting reproductive-age women. The etiopathogenesis and mechanisms of this syndrome remain unclear. Diagnosis requires two of the following: polycystic ovaries, oligo- or anovulation, and hyperandrogenism. Most women with PCOS display conditions such as metabolic abnormalities, diabetes, obesity, cardiovascular disease, and/or bone dysfunction. Considering the ethical limitations of human studies, animal and cell culture models that reflect some features of PCOS are important for investigation of this syndrome. The aim of the present work was to study some of the endocrine relationships between ovaries and bone tissue in a polycystic ovary syndrome animal model. The study was performed using an estradiol valerate PCOS-induced rat model (n = 30) and bone mesenchymal stem cell cultured from bone marrow of those animals. It was hypothesized that changes of the endocrine relationship between ovaries and bones could be observed in from in vivo animal model and in vitro cell culture assays. The ovarian morphological and endocrine changes seem to be correlated with endocrine, biophysical, and biomechanical changes in bone properties. Mesenchymal stem cells obtained from PCOS-induced rats, cultured for up to 21 days and differentiated into osteoblasts, presented lower viability and reduced mineralization of the extracellular matrix. Taken together, these results indicate important endocrine and structural effects of PCOS in ovaries and bones, contributing to part of the understanding of the pathophysiological mechanisms of PCOS.","PeriodicalId":103125,"journal":{"name":"International Journal of Advances in Medical Biotechnology - IJAMB","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122088997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Green synthesis of hydroxyapatite nanoparticles for biomedical application: a brief review 绿色合成羟基磷灰石纳米颗粒的生物医学应用:综述

International Journal of Advances in Medical Biotechnology - IJAMB Pub Date : 2021-03-02 DOI: 10.52466/ijamb.v4i2.96

Andrea Elizabeth Melgar, Ana Paula Fagundes, D. L. P. Macuvele, N. Padoin, C. Soares, H. Riella

{"title":"Green synthesis of hydroxyapatite nanoparticles for biomedical application: a brief review","authors":"Andrea Elizabeth Melgar, Ana Paula Fagundes, D. L. P. Macuvele, N. Padoin, C. Soares, H. Riella","doi":"10.52466/ijamb.v4i2.96","DOIUrl":"https://doi.org/10.52466/ijamb.v4i2.96","url":null,"abstract":"Hydroxyapatite [Ca10(PO4)6(OH)2] (HAp) is a calcium phosphate mineral of great interest in biomaterials development because it is the main component of the mineral phase of bones and teeth’s of animals and humans. HAp plays a key role in the biomedical field due to its non-toxicity, anti-inflammatory property, osteointegrity, biocompatibility, and other properties. Some of its applications in tissue engineering involve bone repair, bone augmentation, coating of implants, and fillers for bones and teeth. Besides, HAp can function as a drug delivery system due to its porous nature, which gives the nanoparticles a larger surface area. However, the use of organic templates in order to obtain HAp nanoparticles with a specific shape, morphology, size, and textural properties makes it impractical from an ecological and cost-effective standpoint. Throughout the last decade, several attempts to improve synthetic procedures have been under development. Recently, special attention has been given to the application of plant extracts as substitutes for organic templates, though they are not extensively reported in literature. Therefore, this brief review aims to examine the existing routes for synthesis of hydroxyapatite nanoparticles. Then the focus shifts to an overview of greener procedures and an outlook of their benefits in biomedical applications. \u0000 ","PeriodicalId":103125,"journal":{"name":"International Journal of Advances in Medical Biotechnology - IJAMB","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116167547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silk: history, obtaining, structure and properties of an old material in the development of new technologies 丝绸:新技术发展中一种旧材料的历史、获取、结构和性能

International Journal of Advances in Medical Biotechnology - IJAMB Pub Date : 2021-03-01 DOI: 10.52466/ijamb.v4i1.88

Roberta Silva Pugina, José Maurício Almeida Caiut

引用次数: 0

Study of the interaction of ionizing radiation in polyurethane polymer films as biomaterial 电离辐射在生物材料聚氨酯聚合物薄膜中的相互作用研究

International Journal of Advances in Medical Biotechnology - IJAMB Pub Date : 2021-03-01 DOI: 10.52466/ijamb.v4i1.97

M. G. Massei, J. Matos, L. Machado, M. Maluf, M. Mathor

{"title":"Study of the interaction of ionizing radiation in polyurethane polymer films as biomaterial","authors":"M. G. Massei, J. Matos, L. Machado, M. Maluf, M. Mathor","doi":"10.52466/ijamb.v4i1.97","DOIUrl":"https://doi.org/10.52466/ijamb.v4i1.97","url":null,"abstract":"New materials are being studied and widely applied in the health area, highlighting biocompatible polymers as the most versatile. Among these polymers, we developed the methodology for the manufacture of Thermoplastic Polyurethane films for application as Biomaterials. The proposed sterilization by ionizing radiation requires the study and characterization of the material to evaluate possible losses or modifications, due to the influence that the radiation can cause in the polymer chains, losing the characteristics for the purpose used. Therefore, the present work evaluates, through chemical and physical-chemical characterization, the possible extension of the changes caused by the radiation in the polyurethane film. The material is produced in an environment with controlled temperature and humidity and subjected to increasing doses of gamma (15, 25 and 50 kGy), ethylene oxide and plasma as comparative techniques. The techniques DSC (Differential Scanning Calorimetry), TGA (Thermogravimetry), FTIR-ATR (Fourier Transform Infrared Spectrometry), SEM (Scanning electron microscopy) and OCT (Optical coherence tomography), have proved that the material, after applied the sterilization techniques, maintains its physical-chemical characteristics and does not suffer any modifications after the treatment.","PeriodicalId":103125,"journal":{"name":"International Journal of Advances in Medical Biotechnology - IJAMB","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124143747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A study on corrosion resistance of ISO 5832-1 austenitic stainless steel used as orthopedic implant 骨科植入物用ISO 5832-1奥氏体不锈钢耐腐蚀性能的研究

International Journal of Advances in Medical Biotechnology - IJAMB Pub Date : 2020-10-01 DOI: 10.25061/IJAMB.V3I2.83

Lilian N. M. Braguin, Caio V. Juvencio da Silva, Larissa O Berbel, I. Costa, M. Saiki

{"title":"A study on corrosion resistance of ISO 5832-1 austenitic stainless steel used as orthopedic implant","authors":"Lilian N. M. Braguin, Caio V. Juvencio da Silva, Larissa O Berbel, I. Costa, M. Saiki","doi":"10.25061/IJAMB.V3I2.83","DOIUrl":"https://doi.org/10.25061/IJAMB.V3I2.83","url":null,"abstract":"The ISO 5832-1 austenitic stainless steel used as biomaterial is largely applied in the area of orthopedics, especially in the manufacture of implants, such as temporary or permanent replacement of bone structures. The objective of this study was to evaluate the localized corrosion resistance of the ISO 5832-1 stainless steel used in orthopedic implants by electrochemical tests in two different solutions. The results of this study are of great interest to evaluate the corrosion of metallic implants that can result in the release of corrosion products into bodily fluids causing possible adverse biological reactions. The determination of the chemical elements in the composition of the ISO 5832-1 stainless steel was performed by neutron activation analysis (NAA). The samples for electrochemical tests were grinded with silicon carbide paper up to #4000 finishing, followed by mechanical polishing with diamond paste. The open circuit potential measurements and anodic polarization curves were obtained in solution of 0.90 wt. % of NaCl and of simulated body fluid (SBF). The results indicated that the ISO 5832-1 stainless steel presented a high resistance to crevice corrosion in simulated body fluid solution but high susceptibility to this form of corrosion in the chloride solution. \u0000 ","PeriodicalId":103125,"journal":{"name":"International Journal of Advances in Medical Biotechnology - IJAMB","volume":"174 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126964777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

PLA membranes loaded with dba analogs. Controlled release study during hydrolitic degradation PLA膜装载dba类似物。水解降解过程的控释研究

International Journal of Advances in Medical Biotechnology - IJAMB Pub Date : 2020-03-01 DOI: 10.52466/ijamb.v4i1.73

GA. Alcántara Blanco, N. Urdaneta, M. Sabino

{"title":"PLA membranes loaded with dba analogs. Controlled release study during hydrolitic degradation","authors":"GA. Alcántara Blanco, N. Urdaneta, M. Sabino","doi":"10.52466/ijamb.v4i1.73","DOIUrl":"https://doi.org/10.52466/ijamb.v4i1.73","url":null,"abstract":"In this research two dibenzylideneacetone (DBA) analogs compounds: (1E,4E)-1-(4-(dimethylamino)phenyl)-5-(4-methoxyphenyl)penta-1,4-dien-3-one (DBA-1) and (1E,4E)-1-(4-methoxyphenyl)-5-(4-nitrophenyl)penta-1,4-dien-3-one (DBA-2) were encapsulated in poly(lactic acid) (PLA) membranes. These DBA analogs can have several applications such as in the development of controlled drug release systems and tissue engineering. The membranes were elaborated by solvent casting. It was found that these fluorescent compounds have a small percentage of hemolysis in human blood red cells at concentrations between 200-500 µg/mL. Therefore, they can be considered not-toxic at these concentrations. The hydrolytic degradation of PLA membranes loaded with the DBA analogs was studied at a temperature of 37 °C under solutions at acid, neutral, and basic pH conditions for a maximum time of six weeks. The hydrolysis was monitored by measuring the loss of mass of the membranes, changes in pH environments, variations in the molecular weight of PLA matrix, and changes in surface morphology observed through Scanning the Electron Microscopy (SEM) technique. Applying UV-visible spectrophotometry, the amount released from the DBA analogs in the PLA membranes was determined during the degradation time, and finally, the release profile was obtained. It was observed employing SEM that the membranes presented a major degradation under basic pH conditions, with a higher percentage of release in an acid medium for both analogues of DBA studied","PeriodicalId":103125,"journal":{"name":"International Journal of Advances in Medical Biotechnology - IJAMB","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128341585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of decellularization of porcine pericardium 猪心包脱细胞的评价

International Journal of Advances in Medical Biotechnology - IJAMB Pub Date : 2020-03-01 DOI: 10.52466/ijamb.v4i1.87

KM. Souza, ALJ. Delgado, A. Santos, ML. Cabrera, M. Miglino

{"title":"Evaluation of decellularization of porcine pericardium","authors":"KM. Souza, ALJ. Delgado, A. Santos, ML. Cabrera, M. Miglino","doi":"10.52466/ijamb.v4i1.87","DOIUrl":"https://doi.org/10.52466/ijamb.v4i1.87","url":null,"abstract":"The porcine pericardium has been used for its great potential as a biological scaffold, produced from the extracellular matrix (ECM) and used mainly in surgeries reconstructive, tissue repair and surgical procedures for corneal reconstruction. The adequate preservation and biocompatibility of the pericardial ECM structure during the decellularization process is fundamental, the biggest challenge being the total removal of cellular material without damage to the structure. All agents used in decellularization change the composition and cause some damage to the ultrastructure. Sodium Dodecil Sulfate (SDS) is the most effective for removing cell residue from tissue compared to other detergents, which is also the most used for the decellularization process. This work aimed to test 3 different concentrations of SDS, in order to assess the concentration (0.1, 0.5 and 1%) that best preserves the structure of the ECM pericardial. In addition, we listed the type of daily wash to make the process more effective (only distilled water or PBS 1x), in order to assess the concentration capable of decellularizing the tissue and better preserving the pericardial ECM. The concentration of SDS at 1%, when compared to the lowest concentrations of 0.1 and 0.5%, was more effective in the decellularization process, however it did not obtain good results in the preservation of the ECM. Regarding daily washing, there was no difference in the frequency assessed in the experimental groups.","PeriodicalId":103125,"journal":{"name":"International Journal of Advances in Medical Biotechnology - IJAMB","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126292363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of calcium phosphates incorporation on structural, thermal and drug-delivery properties of collagen:chitosan scaffolds 磷酸钙掺入对胶原-壳聚糖支架结构、热及给药性能的影响

International Journal of Advances in Medical Biotechnology - IJAMB Pub Date : 2020-02-10 DOI: 10.25061/2595-3931/ijamb/2019.v2i2.32

M. Bertolo, V. Martins, A. Plepis

{"title":"Effects of calcium phosphates incorporation on structural, thermal and drug-delivery properties of collagen:chitosan scaffolds","authors":"M. Bertolo, V. Martins, A. Plepis","doi":"10.25061/2595-3931/ijamb/2019.v2i2.32","DOIUrl":"https://doi.org/10.25061/2595-3931/ijamb/2019.v2i2.32","url":null,"abstract":"In this study, we evaluated how different procedures of calcium phosphate synthesis and its incorporation in collagen:chitosan scaffolds could affect their structural and thermal properties, aiming the obtention of homogeneous scaffolds which can act as drug delivery vehicles in bone tissue engineering. Therefore, three different scaffold preparation procedures were developed, changing the order of addition of the components: in CC-CNPM1 and CC-CNPM2, calcium phosphate synthesis was performed in situ in the chitosan gel (1%, w/w) followed by mixture with collagen (1%, w/w), with changes in the reagents used for calcium phosphate formation; in CC-CNPM3 procedure, calcium phosphate was synthesized ex situ and then incorporated into the collagen gel, in which chitosan in powder was mixed. In all procedures, 5% (in dry mass) of ciprofloxacin was incorporated. FTIR analysis confirmed the presence of calcium phosphate in all scaffolds. DSC curves showed that collagen denaturation temperature (Td) increased with calcium incorporation. SEM photomicrographs of scaffolds cross-section revealed porous scaffolds with calcium phosphate grains internally distributed in the polymeric matrix. XRD diffractograms indicated that the calcium phosphates obtained are hydroxyapatite. The pore size distribution was more homogeneous for CC-CNPM3, which also stood out for its smaller porosity and lower absorption in PBS. These results indicate that the in situ or ex situ phosphate incorporation in the scaffolds had a great influence on its structural properties, which also had consequences for ciprofloxacin release. CC-CNPM3 released a smaller amount of antibiotic (30%), but its release profile was better described by all the tested models.","PeriodicalId":103125,"journal":{"name":"International Journal of Advances in Medical Biotechnology - IJAMB","volume":"210 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114332378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4