The Microbe最新文献

{"title":"Moderately halophilic bacterium Halomonas alkalicola strain Ext as a platform for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer production with fruit peels residues as sole carbon source","authors":"Martin N. Muigano ,&nbsp;Justus M. Onguso ,&nbsp;Sylvester E. Anami ,&nbsp;Godfrey O. Mauti","doi":"10.1016/j.microb.2024.100153","DOIUrl":"10.1016/j.microb.2024.100153","url":null,"abstract":"<div><p>Polyhydroxyalkanoates (PHAs) are synthesized by a variety of microorganisms as intracellular storage granules under imbalanced nutrition and excess carbon. Carbon sources are key contributors to the high cost of PHA production, hence the need for exploration of cheap and sustainable raw materials for bacteria fermentation. In the present study, <em>Halomonas alkalicola</em> strain Ext isolated from a hypersaline lake in Kenya was assessed for its ability to utilize fruit peels hydrolysates (FPH) as sole carbon sources for PHA production. Sugars were extracted from dried peels of banana, mango, orange, and pineapple fruits through mechanical pretreatment and dilute acid hydrolysis. Fruit peels pretreated with 3 % H₂SO₄ at 121℃ were utilized for shake flask fermentation to produce PHAs by <em>Halomonas alkalicola</em> Ext. At optimal C:N ratios of between 20:1 and 30:1, the bacterium could produce up to 0.45±0.03, 0.394±0.12, 0.39±0.05, and 0.28±0.0 g/L of PHAs from hydrolysates of orange peels, mango peels, banana peels, and pineapple peels, respectively. A maximum PHA content of 16.92 % was achieved on orange peels hydrolysates with 4 % substrate loading. Monomer analysis with gas chromatography-mass spectrometry (GC-MS) revealed that the bacterium produced a copolymer Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) with 3-hydroxyvalerate (3HV) contents of 5.77 %, 6.08 %, 6.79 %, 6.845 %, for orange peels, pineapple peels, mango peels and banana peels substrates, respectively. The findings of this study suggest that fruit peels waste is a potential feedstock for sustainable production of PHAs by <em>Halomonas alkalicola</em>.</p></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"4 ","pages":"Article 100153"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2950194624001201/pdfft?md5=9e041df565ae426eb6b2438c12068fde&pid=1-s2.0-S2950194624001201-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142129628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic analysis of the degradation gene clusters and the parABS system in Afipia sp. strain DD3 capable of utilizing 2,4-dichlorophenoxyacetic acid","authors":"Yoriko Sakai","doi":"10.1016/j.microb.2024.100149","DOIUrl":"10.1016/j.microb.2024.100149","url":null,"abstract":"<div><p>Here, I report the complete genome of the soil bacterium <em>Afipia</em> strain DD3, the first representative of its genus to utilize the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) as its sole carbon source. The compact 2,4-D degradation gene cluster of strain DD3 is a set of <em>cadABKC</em> and <em>tfdBFRDEC</em> gene clusters on its chromosome. No <em>tfdA</em> homologs were detected in the genome. The <em>parAB</em> genes responsible for chromosome segregation were found to be close to a candidate chromosomal replication initiation region (<em>oriC</em>) in the chromosome of strain DD3, which is located approximately 180 kb away from <em>dnaA</em>. In 14 strains from five Alphaproteobacterial orders, including strain DD3, <em>parAB</em> was found to be clustered with the genes <em>mnmEG</em> and <em>rsmG</em> coding for tRNA- and rRNA-modifying enzymes. Binding sequences for ParB were found in the promoters of or within these genes, suggesting that <em>parAB</em> genes in Alphaproteobacteria are involved in regulating RNA metabolism during chromosome segregation.</p></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"4 ","pages":"Article 100149"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S295019462400116X/pdfft?md5=f8f9e5a5295e105fa82412e2699a900b&pid=1-s2.0-S295019462400116X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142129627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential and prospects of reductases in azo dye degradation: a review","authors":"Tadele Assefa Aragaw","doi":"10.1016/j.microb.2024.100162","DOIUrl":"10.1016/j.microb.2024.100162","url":null,"abstract":"<div><p>Synthetic dyes, particularly azo dyes, pose environmental and health risks because of their toxicity and persistence. To reduce these negative effects, it is necessary to degrade and detoxify these dyes into simple, non-toxic substances. Enzymes from various organisms play crucial roles in dye degradation and detoxification, making biological treatments cost-effective and environmentally friendly. Reductases are key players in breaking the azo bonds in azo dyes under limited oxygen conditions. This review emphasizes the importance of reductases in azo dye degradation and the exploration of various organisms that produce these enzymes. Azorereductases and NADH-DCIP reductases have been extensively investigated and have been shown to degrade azo dyes efficiently. These enzymes play key roles in azo dye degradation and detoxification by catalyzing the cleavage of azo linkages in the presence of redox coenzymes, such as NADH, NADPH, and FADH₂. However, despite their effectiveness, different conditions, such as pH, temperature, dye concentration, and incubation time, affect enzyme activity during degradation; thus, optimization is required for efficient decolorization. Further studies are necessary to identify new species and strains, isolate and characterize enzymes, and determine the possible pathways for degrading azo dyes.</p></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"4 ","pages":"Article 100162"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2950194624001298/pdfft?md5=c815cd66fb308eb526abe876de23ba62&pid=1-s2.0-S2950194624001298-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142162910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico analysis of drug-resistant genes of Staphylococcus aureus","authors":"Nafyad Ibrahim Batu ,&nbsp;Bayissa Chala Legissa,&nbsp;Geleta Dugassa Barka","doi":"10.1016/j.microb.2024.100165","DOIUrl":"10.1016/j.microb.2024.100165","url":null,"abstract":"<div><p>Drug-resistant strains of pathogenic bacteria are recently considered a devastating health problem. <em>Staphylococcus aureus</em> is a bacterium that commonly lives on the skin and in the nasal passages of approximately one-third of the population without causing any harm. One of the major concerns with <em>Staphylococcus aureus</em> is its ability to develop antibiotic resistance. Drug-resistant genes play an important role in determining the characteristics of bacteria mechanisms of drug resistance. The identification of promoters in a gene is an important part of the recognition of a gene's complete structure. An in silico study was done to analyze the promoter regions and transcription factors with the aid of the transcription start site, regulatory elements, and motifs of genes. Most of the predicted transcription start sites (75 %) were found above 500 bp value either upstream or downstream from the start codon of the gene being expressed. Motif I was identified as the most common motif for the predicted genes, which serves as binding sites to regulate the expression of the genes. A variety of regulatory elements including enhancers, insulators, transcription factors, and cellular proteins are involved in the regulation of gene expression. In general, in silico analysis of drug-resistant genes of <em>Staphylococcus aureus</em> could provide valuable insights about the transcription factor binding, and gene regulatory elements in the promoter regions that can lead to promising research targets in drug designing and development. Therefore, further studies should be made to study resistance-related proteins, the protein-protein interaction networks, the pathways involved in mechanisms underlying resistance, and facilitating the design of target-specific drugs.</p></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"4 ","pages":"Article 100165"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2950194624001328/pdfft?md5=dda3e2b3bc63f06d14233e0a3911b751&pid=1-s2.0-S2950194624001328-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142229054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational exploration of bioactive compounds from Albizia procera: Molecular docking, dynamics, and pharmacokinetics for AchE and BchE inhibition in Alzheimer's disease treatment","authors":"Ekambaram Gayathiri ,&nbsp;Palanisamy Prakash ,&nbsp;Thangaraj Pratheep ,&nbsp;Somdatta Y. Chaudhari ,&nbsp;Subramanian Deepika Priyadharshini","doi":"10.1016/j.microb.2024.100150","DOIUrl":"10.1016/j.microb.2024.100150","url":null,"abstract":"<div><p>Current treatments for Alzheimer's disease (AD) are inadequate and primarily target inhibition of butyrylcholinesterase (BchE) and acetylcholinesterase (AChE) enzymes. This study focused on identifying potential anti-AD compounds from the leaf extract of <em>Albizia procera</em> using gas chromatography-tandem mass spectrometry (GC-MS) analysis and computational approaches. Ethanol seed extract analysis revealed 10 phytoconstituents, which were evaluated for their binding affinities using Autodock Vina and Desmond software against AD-related targets (PDB IDs: 4PQE, 2WJO, and 2POA). Five compounds, along with the control drug, showed significant docking energies. Subsequent in silico ADMET analysis assessed parameters such as intestinal absorption, blood-brain barrier permeability, gastrointestinal absorption, carcinogenicity, and acute oral toxicity. The results extract showed notable affinity for BchE and acetylcholinesterase targets. Molecular dynamics (MD) simulations supported the involvement of hydrogen bonding with ASP207 and GLY75 residues in binding these ligands, demonstrating that disrupting these forces causes destabilization. A ligand plot study also presented a two-dimensional plot of these interactions. The docking results indicate that 7-Oxabicyclo [4.1.0] heptan-2-one could be a promising agent for the treatment of AD because of its higher stability with respect to the two best ligands among all compounds tested. Therefore, from this study, it can be concluded that the compounds present in the leaf extract of A. procera have potential as targets linked to AD and may be helpful for the development of new drugs. Therefore, these data draw a focus on non-synthetic compounds during drug discovery and could possibly provide approaches for exploring existing therapies for AD.</p></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"4 ","pages":"Article 100150"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2950194624001171/pdfft?md5=d1803bfca1bebcd35a9bcff524a109cb&pid=1-s2.0-S2950194624001171-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142097956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of Staphylococcus aureus supernatants in different culture media to mimic chronic wound conditions in vitro","authors":"Lisa-Marie Sittek ,&nbsp;Jana Hunold ,&nbsp;Frank Eugen Runkel ,&nbsp;Peggy Schlupp","doi":"10.1016/j.microb.2024.100161","DOIUrl":"10.1016/j.microb.2024.100161","url":null,"abstract":"<div><p><em>Staphylococcus aureus</em> is a common pathogen found in chronic wounds and it produces virulence factors that inhibit wound healing. There is no standard infection model, so culture supernatants based on different media are used to study the properties of this pathogen. To develop an <em>in vitro</em> infection model, we therefore compared the influence of different culture media on the virulence factors of <em>S. aureus</em> supernatants and their toxicity toward keratinocytes. Supernatants were prepared in brain heart infusion (BHI) broth, lysogeny broth (LB), nutrient broth (NB), and tryptic soy broth (TSB). We assessed bacterial growth, the protein content of the supernatants, and a range of biological activities. SDS-PAGE revealed distinct protein bands consistent with <em>S. aureus</em> hyaluronidase and hemolytic toxins. Hyaluronidase was present in all supernatants and the total activity was similar in BHI, LB, and TSB, but significantly lower in NB. The analysis of hemolytic activity on blood agar and sheep erythrocytes revealed that TSB supernatants showed the highest hemolytic activity closely followed by BHI. Finally, cell viability assays using HaCaT keratinocytes revealed concentration-dependent toxicity for all supernatants, particularly TSB. Our work therefore shows that the culture medium can affect the virulence factors in <em>S. aureus</em> supernatants and also influences their cytotoxicity. These aspects should be considered when using culture supernatants for <em>in vitro</em> studies, and the quantification of virulence factors may improve the relevance and reproducibility of such studies.</p></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"4 ","pages":"Article 100161"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2950194624001286/pdfft?md5=452a132e8d87378b168486daab8f3dce&pid=1-s2.0-S2950194624001286-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142162995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic insights and Taguchi-based optimization of culture conditions for enhanced alkaline protease production in Streptomyces barkulensis RC1831","authors":"Pratyush Kumar Behera ,&nbsp;Zahra Parwez ,&nbsp;Seemon Giri ,&nbsp;Subhransu Sekhar Behera ,&nbsp;Suchismita Nivedita ,&nbsp;Ananta Narayana Panda ,&nbsp;Himadri Tanaya Behera ,&nbsp;Lopamudra Ray","doi":"10.1016/j.microb.2024.100156","DOIUrl":"10.1016/j.microb.2024.100156","url":null,"abstract":"<div><p>This study presents a genome analysis identifying various alkaline protease genes and culture condition optimization to enhance the production of the enzyme in <em>Streptomyces barkulensis</em> RC1831. The analysis of genes using bioinformatics tools such as RAST, KEGG, KAAS, and BLASTx revealed the presence of 10 genes that are responsible for alkaline protease production belonging to different families. Multiple sequence alignment showed a high sequence identity with M24 family serine protease followed by subtilisin-like serine protease. Optimal conditions for enhanced protease production were determined to be at 37°C, pH 11, casein1 % (W/V), dextrose 0.5 % (W/V), urea 0.5 % (W/V), tryptophan 1 % (W/V), 1 mM Mn⁺², 1 % (V/V) Tween-80 in LB medium and an incubation time of 72 hours. Out of the 10 alkaline protease genes, 2 genes expressed significantly according to the activity observed during optimization processes i.e., M24 family, Mn⁺² dependent metalloprotease and subtilisin-like serine, ca⁺² dependent metalloprotease. Furthermore, the AP enzyme was remarkably stable in the presence of various cofactors (Metal ions) and surfactants, indicating its potential for industrial applications under diverse conditions.</p></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"4 ","pages":"Article 100156"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2950194624001237/pdfft?md5=f6fc5ce4e3a7ab86bcbaed9fc4b9ef1b&pid=1-s2.0-S2950194624001237-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142121939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of microbial safety of fresh vegetables through Caenorhabditis elegans model","authors":"Rangasamy Mohanapriya ,&nbsp;Vaikuntavasan Paranidharan ,&nbsp;Subburamu Karthikeyan ,&nbsp;Dananjeyan Balachandar","doi":"10.1016/j.microb.2024.100155","DOIUrl":"10.1016/j.microb.2024.100155","url":null,"abstract":"<div><p><em>Caenorhabditis elegans</em> is widely used as a model to predict the virulence of animal-origin human pathogens, as <em>C. elegans</em> and humans share similar intestinal epithelial cells. A study was undertaken to assess the feasibility of <em>C. elegans</em> for surveillance of foodborne pathogens in fresh vegetables. First, the virulent strains of four foodborne pathogens viz., <em>Pseudomonas aeruginosa</em> (PAO1), <em>Escherichia coli</em> (O157), <em>Salmonella enterica</em> (CI1), and <em>Staphylococcus aureus</em> (ATCC25923) were fed to the worm as sole diet and compared the survival, phenome traits, and gut integrity with standard <em>E. coli</em> OP50. Secondly, the vegetable surface microbiota (tomato) was enriched with gradient concentrations of PAO1, and the worm's survival and gut distension and leakage were assessed. Thirdly, the total microbiota of fresh vegetables (tomato, brinjal, and lablab) were evaluated for the worm's survival. The gut epithelial cell damage and leakage were monitored by blue dye-based smurf assay. Foodborne pathogens reduced the median lifespan (LT₅₀) of the worm drastically, with PAO1 had 2.5 days, O157 (6 days), CI1 (8 days), ATCC25923 (6 days), while the standard diet OP50 had 16 days. The phenome traits of worms, viz., pharyngeal pumping, defecation cycle, body bends, head thrashes, touch stimulus, paralysis, gut leakage, and reproduction, were significantly negatively affected due to pathogen feeding. The gut epithelial integrity was severely affected, and blue dye leakage was the highest in O157 (73 %), followed by PAO1 (55 %), CI1 (40 %) and ATCC25923 (38 %), while the gut epithelial cells were intact in OP50 fed worms. The pathogen enrichment in tomato surface microbiota diet showed that LT₅₀ of the worms was reduced from 8 days to 2.5 days and 10 to 8 days with increasing gradient of PAO1 in fresh tomato microbiota and 24-hours pre-enriched tomato microbiota, respectively. Smurf test also confirmed that an increase in PAO1 proportion in total microbiota distended the gut with high dye leakage. The total microbiota extracted from tomato, brinjal, and lablab did not affect the worm's health and had an extended median life span (11 – 13 days) when they were devoid of any foodborne pathogens. These findings provide a novel and straightforward approach for assessing foodborne pathogens using animal models.</p></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"4 ","pages":"Article 100155"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2950194624001225/pdfft?md5=b078c09af26a4abadc4c5961d7748b18&pid=1-s2.0-S2950194624001225-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142097955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome microsatellite signature analysis in Adenoviridae family of viruses","authors":"Md. Aminur Islam ,&nbsp;Md Gulam Jilani ,&nbsp;Mehboob Hoque ,&nbsp;Safdar Ali","doi":"10.1016/j.microb.2024.100157","DOIUrl":"10.1016/j.microb.2024.100157","url":null,"abstract":"<div><p>Simple sequence repeats (SSRs) are present across both coding &amp; non-coding regions of the genomes of all organisms with diversity in incidence, complexity, and repetition. The present study is focused on <em>Adenoviridae</em> which are non-enveloped viruses consisting of linear double-stranded DNA genome. The members of <em>Adenoviridae</em> are known to cause respiratory illnesses like the common cold (cough, runny nose, mild fever), pneumonia (occasionally), keratoconjunctivitis (infection in the eye known as pink eye), croup and cystitis (inflammation of the bladder). Our investigation aims to extract and analyse SSRs from 68 <em>Adenoviridae</em> genomes. Virus genome sequences were retrieved from NCBI and SSRs extracted from MISA. ETE3 and iTOL were used for the phylogenetic tree construction, annotation and visualization. The genome length of <em>Adenoviridae</em> genomes ranged from 26163 bp to 45667 bp while GC content varied from 33.6 % to 66.9 %. Genome wide analysis revealed a total incidence of 9861 SSRs and 793 cSSRs. The minimum and maximum range of SSR incidence is 112 (A67) to 203 (A61) respectively. The most prevalent mono, di and tri-SSR motif is “A”, “GC/CG” and “GAG/CTC” comprised of 1177, 1859 and 201 occurrences respectively. About 78 % SSRs are present in the coding region in the studied genomes. In terms of protein specific distribution, DNA polymerase enzyme had the highest incidence of 485 SSRs. The presence of mono-SSRs in the A/T region is a marker for host determination and divergence. The average mono-SSRs present in A/T region is 67.14 % and it ranged from 16.22 % to 99.11 %. The high prevalence of mono-SSRs in the A/T region was associated with human and related species as hosts. Further, the clustering of viruses as per their hosts was observed in the phylogenetic tree suggesting the role of host in viral evolution. The presence of unique and conserved cSSRs as genome markers has also been highlighted.</p></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"4 ","pages":"Article 100157"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2950194624001249/pdfft?md5=a46dc16ec6f9b0f24556a43f32d66ced&pid=1-s2.0-S2950194624001249-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142129629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implications and adaptive responses in Avena sativa towards rhizospheric bacteria under drought stress","authors":"Sandeep Kumar Gupta,&nbsp;Riddha Dey,&nbsp;Seema Devi,&nbsp;Richa Raghuwanshi","doi":"10.1016/j.microb.2024.100159","DOIUrl":"10.1016/j.microb.2024.100159","url":null,"abstract":"<div><p>Water deficiency is one of the major abiotic stress conditions that decrease the yields of crops by affecting various metabolic processes in plants. The current state of food security has been alarmingly affected by these circumstances. Consequently, biological approaches to the issue can be implemented while taking into account their positive effects on the environment. The goal of the current work was to obtain rhizospheric bacterial strains possessing stress tolerance abilities, suitable to use as potent plant growth regulator under drought. For this purpose, bacterial strains were isolated from rhizospheric zone of different drought exposed plants and their putative plant growth-promoting attributes were analyzed by morphological and biochemical studies. Among the 30 isolates obtained, the best three drought tolerant and plant growth-promoting strains were selected and identified by 16S rRNA gene sequencing as <em>Enterobacter cloacae</em>-BHUSR1 (MG913373), <em>Serratia marcescens</em>-BHUSR2 (MG913374) and <em>Klebsiella aerogenes-</em>BHUSR3 (MG913375). These bacterial strains were good EPS, IAA, ammonia and siderophores producers as well as nutrient (K, Zn, P) solubilizers. The selected strains were used for biopriming <em>Avena sativa</em> seeds which were further exposed to water stress (5 days) in a completely randomized design to check the bacterial effect on plant growth. Water stress caused a significant alteration in growth, protein and chlorophyll content of <em>Avena sativa</em>. However, biopriming improved the physiological conditions of the oat plants by increasing the osmolytes (proline, phenol and sugar) under water deficient conditions. This technique was even helpful in augmenting the plants antioxidant levels, as high catalase activity was observed in oat after biopriming. It can be concluded from the study that bacterial seed biopriming can be a helpful tool in overcoming drought effects, as it regulates the plants physiological response towards stress with <em>Klebsiella aerogenes</em>-BHUSR3 being an efficient plant growth promoter in water deficient soils.</p></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"4 ","pages":"Article 100159"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2950194624001262/pdfft?md5=a7edbb777d87075b5099cac45d2308bc&pid=1-s2.0-S2950194624001262-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142162996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}