Combination of curcumin or chitosan with photodynamic therapy as an effective alternative therapy for overcoming wound infection associated with multidrug-resistant Acinetobacter baumannii

The Microbe Pub Date : 2025-05-24 DOI:10.1016/j.microb.2025.100375

Manta Yonpiam , Dhammika Leshan Wannigama , Aye Mya Sithu Shein , Tingting Liao , Cameron Hurst , Peter N. Monk , Mohan Amarasiri , Phatthranit Phattharapornjaroen , Puey Ounjai , Thammakorn Saethang , Vishnu Nayak Badavath , Sirirat Luk-in , Sumanee Nilgate , Ubolrat Rirerm , Sukrit Srisakul , Natharin Ngamwongsatit , Asada Leelahavanichkul , Naveen Kumar Devanga Ragupathi , Talerngsak Kanjanabuch , Phurin Narkban , Tanittha Chatsuwan

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Abstract

The increasing prevalence of multidrug-resistant Acinetobacter baumannii as an opportunistic pathogen in wound infections raises significant concerns due to its antibiotic resistance and biofilm-mediated antibiotic tolerance. This underscores the urgent need to explore an alternative approach to effectively managing wound infections caused by MDR A. baumannii. In this study, combining subinhibitory concentrations of curcumin (25 µg/ml) or chitosan (156 µg/ml) with photodynamic therapy-PDT (10 J/cm²) resulted in a significant reduction of planktonic viabilities and biofilm biovolume of MDR A. baumannii clinical isolates in vitro. In murine A. baumannii-associated wound infection, a combination of curcumin (25 µg/ml) or chitosan (156 µg/ml) with PDT (10 J/cm²) resulted in complete bacterial eradication with wound healing at Day 15 of post-treatments with these combination therapies. Our study demonstrated that combining curcumin or chitosan with PDT at their subinhibitory concentrations represents a promising alternative strategy as localized therapy for effectively managing A. baumannii-associated wound infection.

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姜黄素或壳聚糖联合光动力治疗是克服多药耐药鲍曼不动杆菌引起的伤口感染的有效替代疗法

多药耐药鲍曼不动杆菌作为伤口感染的机会致病菌日益流行，由于其抗生素耐药性和生物膜介导的抗生素耐受性，引起了人们的极大关注。这强调了迫切需要探索一种有效管理由耐多药鲍曼杆菌引起的伤口感染的替代方法。在本研究中，将姜黄素（25 µg/ml）或壳聚糖（156 µg/ml）的亚抑制浓度与光动力疗法- pdt（10 J/cm2）联合使用，可显著降低MDR鲍曼不动杆菌临床分离株的体外浮游活力和生物膜生物体积。在小鼠鲍曼不雅杆菌相关伤口感染中，姜黄素（25 µg/ml）或壳聚糖（156 µg/ml）与PDT（10 J/cm2）联合治疗后15天，细菌完全根除，伤口愈合。我们的研究表明，将姜黄素或壳聚糖与亚抑制浓度的PDT联合使用是一种有希望的替代策略，可以有效地治疗鲍曼杆菌相关伤口感染。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Microbe

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