The Microbe最新文献

{"title":"Investigation of the effect of probiotics and specific bacteriophages on the biofilm formed by Streptococcus mutans","authors":"Mohammad Mehdi Soltan Dallal ,&nbsp;Mohammad Reza Afradi ,&nbsp;Zahra Rajabi ,&nbsp;Mahdieh Sepehri","doi":"10.1016/j.microb.2025.100250","DOIUrl":"10.1016/j.microb.2025.100250","url":null,"abstract":"<div><div>This study investigates the effect of probiotics and specific bacteriophage on the biofilm formed by <em>Streptococcus mutans</em>, a bacterium strongly associated with tooth decay. Dental plaque and sewage were used to isolate <em>S</em>. <em>mutans</em> and bacteriophages, respectively. Three probiotic lactobacilli (<em>L. plantarum</em>, <em>L. acidiphilus</em>, and <em>L. casei</em>) previously isolated in our studies were used.</div><div><em>S. mutans was</em> confirmed by biochemical and molecular tests. PCR was used to detect the gtfB and gtfC genes. Bacteriophage morphology was studied using phages extracted through the double-layer agar method and TEM. The effect of probiotics and lytic bacteriophage on the formation of <em>S</em>. <em>mutans</em> biofilm was investigated by the colorimetric method with crystal violet and the real-time PCR method. Out of 60 dental plaque samples, 21 (35 %) were identified as <em>S. mutans</em>. All of them contain <em>the gtfD</em> gene, and the frequency of other genes was as follows: <em>gtfB</em> 38 (63.33 %), <em>gtfC</em> 33 (55 %). <em>S. mutans</em> had strong power to form biofilm. The impact of Lactobacilli cell-free supernatant and bacteriophage on the growth and biofilm formation of isolated <em>S. mutans</em> resulted in a significant reduction in biofilm formation and gene expression levels. The inhibitory effect of bacteriophage was the most effective. The study's results indicate that bacteriotherapy, which involves the use of probiotics and specific bacteriophages against <em>S. mutans</em>, could be a promising approach to combat and reduce the formation of biofilms by <em>S. mutans</em>, as well as to decrease dental plaque and dental disease</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"6 ","pages":"Article 100250"},"PeriodicalIF":0.0,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Examining alternative approaches to antibiotic utilisation: A critical evaluation of phage therapy and antimicrobial peptides combination as potential alternatives","authors":"Chibuzo Vincent Alisigwe ,&nbsp;Chibuzor Stanley Ikpa ,&nbsp;Uchenna Joseph Otuonye","doi":"10.1016/j.microb.2025.100254","DOIUrl":"10.1016/j.microb.2025.100254","url":null,"abstract":"<div><div>Antimicrobial peptides (AMPs) and bacteriophages, though long recognised, have gained significant attention in recent antimicrobial research due to the rapid evolution of microbial resistance to existing antibiotics. This publication comprehensively examines the current literature on the potential of AMPs and bacteriophages to treat infections caused by antimicrobial-resistant bacteria, either as monotherapies, in combination with existing antibiotics, or in synergy with each other. Bacteriophages kill bacteria by injecting their genomes (DNA or RNA), leading to bacterial cell lysis, while AMPs can either disrupt bacterial cell membranes or interfere with their intracellular targets after penetrating the cell, causing cell death. Our review highlights that both AMPs and bacteriophages have been successfully employed in vitro, in clinical trials, and in patient treatments to eliminate bacterial isolates. Notably, studies combining AMPs and bacteriophages, though not yet widespread, have demonstrated remarkable efficacy. For example, one study investigated the combined use of Lys11, an endolysin derived from phage ϕ11, and the AMP R8K against <em>Staphylococcus aureus</em>. The results indicated that pre-treatment with R8K significantly increased the susceptibility of <em>S. aureus</em> to Lys11-mediated lysis, even at low concentrations. While the precise mechanisms underlying the synergistic action of AMPs and bacteriophages remain unclear, our findings underscore the need for further research in this promising domain. Regarding safety, numerous studies affirm the general safety of bacteriophages due to their specificity for bacterial cells. However, concerns about phage evolution potentially eliciting human immune responses necessitate additional investigation to enhance phage therapy. The issue of safety also extends to the recommended purification of AMPs when used in conjunction with bacteriophages to prevent adverse immune reactions in patients. In conclusion, this review advocates for intensified research into the combined use of AMPs and bacteriophages, emphasising their potential to revolutionise the treatment of antimicrobial-resistant infections.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"6 ","pages":"Article 100254"},"PeriodicalIF":0.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of microbial diversity and antimicrobial resistance in raw camel milk: Genomic and phenotypic analysis of Kharai and Kutchi breeds of Gujarat, India","authors":"Hetvi J. Adhyaru,&nbsp;Devangi B. Mangroliya,&nbsp;Jayesh H. Kabariya,&nbsp;Vimal M. Ramani","doi":"10.1016/j.microb.2025.100245","DOIUrl":"10.1016/j.microb.2025.100245","url":null,"abstract":"<div><div>Camel milk is increasingly recognized for its nutritional and therapeutic properties, especially in regions like India, which ranks seventh globally in camel population. This study focused on analyzing the microbiome and antimicrobial resistome in raw camel milk from two unique breeds, Kharai and Kutchi, found in Gujarat's Kutch district. Both breeds exhibit unique microbial compositions, with the Kharai breed having a more diverse bacterial community. Through phenotypic antimicrobial resistance analysis using the disc diffusion method, a significant number of multidrug-resistant (MDR) and extensively drug-resistant (XDR) bacterial isolates were identified. The genotypic analysis, performed via nanopore metagenomic sequencing, revealed the presence of various antimicrobial resistance genes (ARGs). In the Kharai breed 58 ARGs were detected, majority of them conferring resistance to fluoroquinolones, tetracycline, macrolides, and colistin. In contrast, 13 ARGs were detected in the Kutchi breed, with a focus on resistance to fluoroquinolones and spectinomycin. In addition to pathogenic bacteria, beneficial lactic acid bacteria such as <em>Lactobacillus</em> and <em>Enterococcus</em> were identified in the Kharai breed milk, contributing to its probiotic potential. However, the diversity of pathogenic bacteria was very low as compared to bovine milk, including <em>Acinetobacter</em>, <em>Staphylococcus</em>, <em>Klebsiella</em>, and <em>Salmonella</em>, in both breeds. Importantly, the study found that camel milk harboured fewer ARGs related to last-resort antibiotics, such as β-lactams, compared to bovine milk. This suggests that camel milk poses a lower risk compared to other raw milk sources with respect to spread of antimicrobial resistance (AMR). The findings underline the need for monitoring camel milk to safeguard public health. This comprehensive assessment of both genotypic and phenotypic AMR profiles in raw camel milk provides crucial insights into its microbial diversity and spread of antimicrobial resistance.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"6 ","pages":"Article 100245"},"PeriodicalIF":0.0,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of fecal bacteriome of diarrhoeic and non-diarrhoeic calves revealed diversified community structures","authors":"Zebunnahar Yasmin ,&nbsp;Mohammad Alamgir Hossain ,&nbsp;Sharmin Chowdhury ,&nbsp;Md. Habib Ullah Masum ,&nbsp;Md. Shaminur Rahman ,&nbsp;M. Nazmul Hoque ,&nbsp;AMAM. Zonaed Siddiki","doi":"10.1016/j.microb.2025.100251","DOIUrl":"10.1016/j.microb.2025.100251","url":null,"abstract":"<div><div>The maintenance of healthy gut microbiota is of utmost significance when it comes to combating gastrointestinal disorders like diarrhoea. This study was designed to unveil the gut bacteriome signature and diversity by analyzing five diarrhoeic (DF) and five non-diarrhoeic feces (NDF) samples obtained from 10 individual calves through 16S rRNA (V4 region) gene-based amplicon sequencing. Our findings revealed substantial taxonomic variability between DF and NDF (p = 0.0127), indicated by their higher degree of shared microbiota. Among the identified genera, <em>Gallibacterium</em> and <em>Veillonella</em> were the major bacterial genera detected in the gut of calves, with marked discrepancies in their relative abundances in DF and NDF. We successfully identified 44 genera, including <em>Sedimentibacter</em>, <em>Lonepinella</em>, <em>Sulfurospirillum</em>, <em>Haemophilus</em>, and <em>Enterobacter</em>. which are linked to calf diarrhoea. The DF and NDF included 264 distinct bacterial species, of which 43.56 % were shared between sample categories, and 38.63 % and 17.80 % were found solely in DF and NDF, respectively. Moreover, <em>Gallibacterium salpingitidis</em> (43.37 %) was the most prevalent species in DF samples, followed by <em>Gallibacterium anatis</em> (17.56 %). In contrast, <em>Veillonella magna</em> (19.21 %) had the highest prevalence in NDF samples, followed by <em>Bacteroides</em> sp. (18.00 %). The findings suggested that diarrhoea affects the gut bacteriome in calves, with evidence for the existence of both unique and shared bacteriomes with pathogenic implications in the gut of calves. We expect that upcoming innovations will pave the way for personalized, microbiota-centered therapies, presenting hopeful approaches to influence microbial populations for better health results effectively.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"6 ","pages":"Article 100251"},"PeriodicalIF":0.0,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbapenem-Resistant Klebsiella pneumoniae: A comprehensive review of phenotypic and genotypic methods for detection","authors":"Darya Mohammadpour ,&nbsp;Mohammad Yousef Memar ,&nbsp;Hamed Ebrahimzadeh Leylabadlo ,&nbsp;Anahita Ghotaslou ,&nbsp;Reza Ghotaslou","doi":"10.1016/j.microb.2025.100246","DOIUrl":"10.1016/j.microb.2025.100246","url":null,"abstract":"<div><div><em>Klebsiella pneumoniae (K. pneumoniae)</em> is a non-motile Gram-negative bacterium in the <em>Enterobacteriaceae</em> family. The rise of antibiotic resistance, especially carbapenem-resistant <em>K. pneumoniae</em> (CRKP), poses a serious public health threat due to its multidrug-resistant nature and high mortality rates. This study aims to provide an overview of phenotypic and genotypic methods for identifying CRKP. The study found that Carba NP, MCNP, and eCIM are highly sensitive phenotypic tests, while Carba NP, MCNP, eCIM, and mCIM are the most specific. Among genotypic methods, Multiplex PCR, LAMP, and q-PCR are the most sensitive, and Multiplex PCR, PCR, and q-PCR are the most specific. Conventional methods' limitations have delayed timely pathogen analysis, emphasizing the need for quick and accurate CRKP identification in clinical settings for detection and infection control.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"6 ","pages":"Article 100246"},"PeriodicalIF":0.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Essential oils used in the poultry industry: Would it be an effective green alternative against Salmonella spp. dissemination and antimicrobial resistance?","authors":"Heitor Leocádio de Souza Rodrigues,&nbsp;Isis Mari Miyashiro Kolososki,&nbsp;Valdinete Pereira Benevides,&nbsp;Mauro M.S. Saraiva,&nbsp;Angelo Berchieri Junior","doi":"10.1016/j.microb.2025.100248","DOIUrl":"10.1016/j.microb.2025.100248","url":null,"abstract":"<div><div>Due to the increasing emergence of antimicrobial-resistant strains of <em>Salmonella</em> spp. isolated from poultry, and the global trend towards reducing antimicrobial use in food-producing animals, alternatives to these drugs are being sought. Among these alternatives, the antimicrobial action of essential oils (EOs) stands out. These compounds are plant-derived compounds with antimicrobial effectiveness against Gram-positive and Gram-negative bacteria, including strains of <em>Salmonella</em>. EOs are employed in the food industry due to their aromatic and antimicrobial properties and their role as natural preservatives as well. However, studies have been approaching their weight gain and performance instead of focusing on the applicability of these compounds in poultry challenged with avian salmonellae or other bacterial diseases. Therefore, the present study aimed to review the pros and cons of using EOs against <em>Salmonella</em> spp. in poultry. Although there are reports of the antimicrobial effectiveness of EOs against <em>Salmonella</em> spp. both <em>in vitro</em> and <em>in vivo</em>, their use for this purpose has not been deeply studied. Until then, optimal concentrations for controlling <em>Salmonella</em> shedding or toxic concentrations for poultry have not been established. On the other hand, it is known that these products can exhibit synergistic effects with other antimicrobials. Thus, investigations related to the pharmacokinetics, mechanisms of action, and adverse effects of EOs in the poultry's body are important to elucidate the treatment with these alternative antimicrobials, as well as to understand their interactions with both pathogenic bacteria and the bacteria that naturally compose the poultry's microbiota.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"6 ","pages":"Article 100248"},"PeriodicalIF":0.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial susceptibility patterns of pathogens isolated from clinical specimens at a tertiary care hospital in Bangladesh, 2020–2023","authors":"Mohammad Kamruzzaman ,&nbsp;Fahim Alam Nobel ,&nbsp;Saiful Islam ,&nbsp;Amiyo Haider ,&nbsp;Mohammad Nasir Uddin ,&nbsp;Mohammod Johirul Islam","doi":"10.1016/j.microb.2025.100244","DOIUrl":"10.1016/j.microb.2025.100244","url":null,"abstract":"<div><div>Antimicrobial resistance has emerged as a critical issue in the medical sector globally, including in developing countries such as Bangladesh. The objective of the study was to determine the profile of bacteria and antibiotic resistance patterns of various clinical specimens at a tertiary care hospital in Mymensingh, Bangladesh from January 2020 to December 2023. In this study, a total of 2068 samples were collected from various clinical specimens, such as ear, wound, throat, urine, and pus, following standardized collection procedures. The isolates were characterized through colony morphology, gram staining, and biochemical assays. Their antibiotic susceptibility patterns were determined using the Kirby-Bauer disk diffusion method. Out of 2068 isolates, 644 (31.1 %) were gram-positive and 1424 (68.9 %) were gram-negative bacteria. Isolates belonged to males (55.3 %) and females (44.7 %). The most common gram-negative bacterial isolates were <em>Pseudomonas</em> spp. (33.9 %) and <em>Escherichia coli</em> (23.4 %), whereas the most common isolates of gram-positive bacteria were <em>Staphylococcus</em> spp. (15.8 %) and <em>Streptococcus</em> spp. (12.2 %). Among gram-positive bacteria, resistance was highest to Penicillin-G (92 %) and Colistin (90.8 %). Gram-negative bacteria showed the highest resistance to Colistin (83.3 %) and Cefuroxime (82.1 %). The majority of the isolates were resistant to multiple antibiotics (≥3), with 83.4 % being multidrug-resistance (MDR), 8.7 % extensively drug-resistance (XDR), and 0.6 % pan-drug resistance (PDR). Given the rising antimicrobial resistance in Bangladesh, regular surveillance to monitor bacterial patterns and antibiotic sensitivity is essential for developing local antibiograms, which can guide physicians in effectively treating infections.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"6 ","pages":"Article 100244"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic investigation of an outbreak of febrile illness with rashes in the eastern Uttar Pradesh, India during March-May 2023","authors":"Sthita Pragnya Behera ,&nbsp;Nalini Mishra ,&nbsp;Aishwarya Shukla,&nbsp;Moni Kumari,&nbsp;Sonal Rajput,&nbsp;Imbisat Fatma,&nbsp;Ashutosh Tiwari,&nbsp;Rajeev Singh,&nbsp;Rajni Kant,&nbsp;Gaurav Raj Dwivedi","doi":"10.1016/j.microb.2025.100243","DOIUrl":"10.1016/j.microb.2025.100243","url":null,"abstract":"<div><div>Various infectious diseases cause febrile illness with rash. Among these, measles and varicella zoster virus (VZV) infections are ubiquitous viral infections with classic symptoms of fever and rashes. During March-May 2023, an outbreak of fever with rashes was reported from five villages in the Gorakhpur district of Uttar Pradesh. Clinical specimens were collected from suspected patients (n = 42) and tested for Anti-measles IgM and Anti-VZV IgM. In addition to this, DNA from VZV-positive samples were amplified for ORF22, ORF38, ORF54, and glycoprotein B genes, to know the prevalent genotype. Of the total number of cases, thirty-three (78 %) cases were confirmed to be infected with VZV, detected either by PCR (27,81 %) or IgM ELISA (6,19 %), and five (11.9 %) co-infected cases were established through the presence of IgM antibodies for both (VZV and measles virus). Amplification of ORF38 and ORF54 genes of all VZV-infected patients were found to be positive for both the markers (<em>BglI</em>+ and <em>PstI</em>+). Further genetic analysis of ORF22 and glycoprotein B gene revealed clade 4/5 &amp; clade 1/3 genotypes of VZV are responsible for the outbreak. These findings will be useful for future surveillance and formulation of policies to the immunization for VZV and measles.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"6 ","pages":"Article 100243"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leishmaniasis: A multifaceted approach to diagnosis, maladies, drug repurposing and way forward","authors":"Naureenbanu Makarani ,&nbsp;Krupanshi Bharadava ,&nbsp;Aviral Kaushik ,&nbsp;Ayush Dave ,&nbsp;Ajit K. Gangawane ,&nbsp;Radhey Shyam Kaushal","doi":"10.1016/j.microb.2025.100239","DOIUrl":"10.1016/j.microb.2025.100239","url":null,"abstract":"<div><div>More than 20 species of the protozoan parasite Leishmania cause leishmaniasis, a disease spread by sand flies. The WHO lists leishmaniasis as one of the most ubiquitous neglected tropical illnesses. As per the reports, more than 1.3 million cases worldwide result in 20,000–30,000 fatalities every year. Although the global burden of disease is not rising, there remains a chance that it could spread. Leishmaniasis is predominantly detected in Brazil, Ethiopia, India, Kenya, Somalia, South Sudan, and Sudan, with transmission occurring through bites from infected phlebotomine sand flies to humans and other hosts. Pathogenesis of leishmaniasis based on the pathogen or host mechanism. The diagnosis of leishmaniasis is distinguished by clinical pleomorphism confirmation and also done by parasitological methods which are highly specific and sensitive. Despite the favoring of drugs such as Amphotericin B, sodium stibogluconate IV and meglumine IM (antimonial), miltefosine, paromomycin, and pentamidine, specific treatments, medications, and vaccination are still lacking for this neglected disease. Consequently, the drug repurposing-based approach has been adopted to fill this gap. The control measures vary from region to region. This review article describes the epidemiology, clinical aspects featuring the diagnostic and therapeutic techniques along with the benefits and challenges of repurposing drugs. Drugs of various categories have been repurposed to treat leishmaniasis, for instance, Clotrimazole, Nystatin (antifungal); Escitalopram, Imipramine (antidepressant); Paromomycin, Pentamidine (antibiotics); Sunitinib, Lapatinib (anticancer) and many more. The study focuses on the necessity of thorough validation, pharmacological constraints, and regulatory requirements by utilizing advances in computational biology and screening, which can be used as a promising treatment.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"6 ","pages":"Article 100239"},"PeriodicalIF":0.0,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolites profiling based antioxidant, anti-cancer cell proliferation activity and molecular docking and stimulation studies of Parthenium hysterophorus L.","authors":"Vellaiyan Sathish ,&nbsp;Ekambaran Gayathiri ,&nbsp;Thangaraj Pratheep ,&nbsp;Palanisamy Prakash ,&nbsp;Subramaniam Umavathi","doi":"10.1016/j.microb.2025.100238","DOIUrl":"10.1016/j.microb.2025.100238","url":null,"abstract":"<div><div>Medicinal plants (MPs) are gaining significance owing to their wealth of bioactive phytochemicals<em>. P. hysterophorus</em> L. is a belonging to the Asteraceae family. The wide range of bioactive substances has led to a greater need for medicinal plants for the treatment of different ailments, including inflammatory conditions, diabetes, and cancer. The plant antioxidant activity in the DPPH and ABTs assays, along with encouraging as assessed by the anti proliferative assay CADD based studies. Moreover, in this study, we highlighted the importance of in silico analysis, which evaluates the anticancer effectiveness of the experiment examines its pharmacokinetics based on molecular docking and dynamics. Phytocompunds derived from <em>P. hysterophorus</em> L were subjected to molecular docking (EGFR and cytochrome p50) complex in cancer. The docking binding scores, with the most favorable score recorded at -9.2 kcal/mol. Molecular stimulation stability of the ligand-protein complexes was supported throughout the simulation. Based on this investigation, these ligands showed promising anticancer activity. Moreover, a thorough examination of laboratory and computational data indicated that the leaf has the potential to be a strong medicinal substance and might be used as a successful treatment in the future.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"6 ","pages":"Article 100238"},"PeriodicalIF":0.0,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}