从伊朗土壤中分离的强效益生菌Mojavensis微囊化及其特性测定

Zahra Karimi Dastjerdi, Shokoofeh Ghazi
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摘要

益生菌是一种活的微生物,它存在于食物中,通过改善消化系统的微生物菌群,对宿主有有益的影响。细菌包封保护脆弱的细菌免受不利条件的影响,提高它们的稳定性。因此,本研究的目的是从德黑兰东北部土壤中分离出耐药的mojavensis益生菌,并用海藻酸钠包封以提高其稳定性。本研究在Jamshidiyeh公园随机抽取5个土壤样本。采用连续稀释法制备法和热处理法对土壤样品进行了分离。对培养菌落进行纯化和孢子染色后,进行过氧化氢酶和氧化酶试验,确定芽孢杆菌属,然后进行耐酸、耐盐、胆盐试验和耐抗生素试验,确定纯化菌种的益生菌。为了更准确地确定具有益生菌特征的优越属和种,进行了分子鉴定(测序16srRNA)。然后用海藻酸钠包封芽孢杆菌。对微胶囊的形成进行验证性分析,包括扫描电镜摄影和DLS分析,以检查微胶囊的形成、颗粒大小、微胶囊计数和稳定性。共分离到33株 g阳性芽孢杆菌,其中7株具有益生菌特性。最终,我们选择了具有所有益生菌特性的菌株(ZK)1作为指示菌株。16-srRNA测序结果显示,目标菌株属于芽孢杆菌mojavensis MK764986属和种,相似率为99.8 %。海藻酸钠微胶囊化结果证实,细胞直径在5到12微米之间,均匀分布。该研究结果表明,该国的原生土壤生态系统及其分离的芽孢杆菌菌株是生产和引入有价值的非致病性原生细菌的合适来源,是益生菌的新来源。在本研究中,我们发现包封的芽孢杆菌具有保存期长等优点,在证明其益生菌特性后,可以用于食品和药品中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microencapsulation of Bacillus Mojavensis as potent probiotic strain isolated from soil of Iran and determining characterization
Probiotics are living microbes whose presence in food has beneficial effects for the host by improving the digestive system's microbial flora. Bacterial encapsulation safeguards delicate bacteria from adverse conditions and enhances their stability. Hence, the aim of this research was to isolate resistant Bacillus mojavensis as a probiotic from North East Tehran soil and encapsulated using sodium alginate for increasing its stability. In this study, five soil samples from Jamshidiyeh Park were taken randomly. The soil samples were tested using the serial dilution preparation method and heat treatment to isolate the Bacillus genus exclusively. Following the purification of grown colonies and spore staining, catalase and oxidase tests were performed to confirm the genus of Bacillus sp., and then tests such as acid resistance, salt, bile salt test, and antibiotic resistance were performed to confirm the probiotics of the purified species. In order to determine more precisely the superior genus and species with probiotic characteristics, molecular identification (sequencing 16srRNA) was performed. Next, sodium alginate was used to encapsulate the Bacillus strain. Confirmatory analyzes of microcapsule formation, including photography with an SEM electron microscope and using DLS analysis, were performed to check the confirmation of microcapsule formation, particle size, microcapsule counting, and stability. Overall, we isolated 33 g-positive Bacillus sp., of which 7 strains showed probiotic properties. Ultimately, we selected the strain named (ZK)1 as the indicator strain, exhibiting all probiotic properties. With a 99.8 % similarity rate, 16-srRNA sequencing showed that the target strain belonged to the genus and species Bacillus mojavensis MK764986. The microencapsulation with sodium alginate results confirmed the cells were between 5 and 12 micrometers across and spread out evenly. The study's findings indicated that the country's native soil ecosystem and the Bacillus strains isolated from it serve as a suitable source for the production and introduction of valuable, non-pathogenic native bacteria, serving as a new source of probiotic bacteria. In this research, we found that encapsulated Bacillus sp. can be used in food and pharmaceutical products after proving their probiotic properties because of advantages such as high shelf life.
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