The Microbe最新文献_第2页

Antimicrobial susceptibility patterns of pathogens isolated from clinical specimens at a tertiary care hospital in Bangladesh, 2020–2023

The Microbe Pub Date : 2025-01-20 DOI: 10.1016/j.microb.2025.100244

Mohammad Kamruzzaman , Fahim Alam Nobel , Saiful Islam , Amiyo Haider , Mohammad Nasir Uddin , Mohammod Johirul Islam

{"title":"Antimicrobial susceptibility patterns of pathogens isolated from clinical specimens at a tertiary care hospital in Bangladesh, 2020–2023","authors":"Mohammad Kamruzzaman , Fahim Alam Nobel , Saiful Islam , Amiyo Haider , Mohammad Nasir Uddin , Mohammod Johirul Islam","doi":"10.1016/j.microb.2025.100244","DOIUrl":"10.1016/j.microb.2025.100244","url":null,"abstract":"<div><div>Antimicrobial resistance has emerged as a critical issue in the medical sector globally, including in developing countries such as Bangladesh. The objective of the study was to determine the profile of bacteria and antibiotic resistance patterns of various clinical specimens at a tertiary care hospital in Mymensingh, Bangladesh from January 2020 to December 2023. In this study, a total of 2068 samples were collected from various clinical specimens, such as ear, wound, throat, urine, and pus, following standardized collection procedures. The isolates were characterized through colony morphology, gram staining, and biochemical assays. Their antibiotic susceptibility patterns were determined using the Kirby-Bauer disk diffusion method. Out of 2068 isolates, 644 (31.1 %) were gram-positive and 1424 (68.9 %) were gram-negative bacteria. Isolates belonged to males (55.3 %) and females (44.7 %). The most common gram-negative bacterial isolates were <em>Pseudomonas</em> spp. (33.9 %) and <em>Escherichia coli</em> (23.4 %), whereas the most common isolates of gram-positive bacteria were <em>Staphylococcus</em> spp. (15.8 %) and <em>Streptococcus</em> spp. (12.2 %). Among gram-positive bacteria, resistance was highest to Penicillin-G (92 %) and Colistin (90.8 %). Gram-negative bacteria showed the highest resistance to Colistin (83.3 %) and Cefuroxime (82.1 %). The majority of the isolates were resistant to multiple antibiotics (≥3), with 83.4 % being multidrug-resistance (MDR), 8.7 % extensively drug-resistance (XDR), and 0.6 % pan-drug resistance (PDR). Given the rising antimicrobial resistance in Bangladesh, regular surveillance to monitor bacterial patterns and antibiotic sensitivity is essential for developing local antibiograms, which can guide physicians in effectively treating infections.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"6 ","pages":"Article 100244"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic investigation of an outbreak of febrile illness with rashes in the eastern Uttar Pradesh, India during March-May 2023

The Microbe Pub Date : 2025-01-20 DOI: 10.1016/j.microb.2025.100243

Sthita Pragnya Behera , Nalini Mishra , Aishwarya Shukla, Moni Kumari, Sonal Rajput, Imbisat Fatma, Ashutosh Tiwari, Rajeev Singh, Rajni Kant, Gaurav Raj Dwivedi

{"title":"Genomic investigation of an outbreak of febrile illness with rashes in the eastern Uttar Pradesh, India during March-May 2023","authors":"Sthita Pragnya Behera , Nalini Mishra , Aishwarya Shukla, Moni Kumari, Sonal Rajput, Imbisat Fatma, Ashutosh Tiwari, Rajeev Singh, Rajni Kant, Gaurav Raj Dwivedi","doi":"10.1016/j.microb.2025.100243","DOIUrl":"10.1016/j.microb.2025.100243","url":null,"abstract":"<div><div>Various infectious diseases cause febrile illness with rash. Among these, measles and varicella zoster virus (VZV) infections are ubiquitous viral infections with classic symptoms of fever and rashes. During March-May 2023, an outbreak of fever with rashes was reported from five villages in the Gorakhpur district of Uttar Pradesh. Clinical specimens were collected from suspected patients (n = 42) and tested for Anti-measles IgM and Anti-VZV IgM. In addition to this, DNA from VZV-positive samples were amplified for ORF22, ORF38, ORF54, and glycoprotein B genes, to know the prevalent genotype. Of the total number of cases, thirty-three (78 %) cases were confirmed to be infected with VZV, detected either by PCR (27,81 %) or IgM ELISA (6,19 %), and five (11.9 %) co-infected cases were established through the presence of IgM antibodies for both (VZV and measles virus). Amplification of ORF38 and ORF54 genes of all VZV-infected patients were found to be positive for both the markers (<em>BglI</em>+ and <em>PstI</em>+). Further genetic analysis of ORF22 and glycoprotein B gene revealed clade 4/5 & clade 1/3 genotypes of VZV are responsible for the outbreak. These findings will be useful for future surveillance and formulation of policies to the immunization for VZV and measles.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"6 ","pages":"Article 100243"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro exploration of the multi-functional dermatological benefits of a Lactobacillus-turmeric synbiotic

The Microbe Pub Date : 2025-01-17 DOI: 10.1016/j.microb.2025.100242

Jinal Bhola , Rama Bhadekar

{"title":"In vitro exploration of the multi-functional dermatological benefits of a Lactobacillus-turmeric synbiotic","authors":"Jinal Bhola , Rama Bhadekar","doi":"10.1016/j.microb.2025.100242","DOIUrl":"10.1016/j.microb.2025.100242","url":null,"abstract":"<div><div>Skin conditions such as acne, eczema, and dandruff affect a significant portion of the population, with conventional treatments often facing challenges like side effects, high cost, and resistance development. The need for safer, natural alternatives has driven research into probiotics and bioactive compounds. This study focuses on addressing these issues by developing an Synbiotic Bioactive Lysate (SBL), by combining <em>Lactobacillus</em> strains with turmeric extract. The antimicrobial efficacy of SBL was assessed against common skin and scalp pathogens like <em>Staphylococcus</em> sp., <em>Propionibacterium</em> sp., <em>Malassezia</em> sp., and Candida sp. using agar well diffusion methods. In addition, enzyme inhibition assays and anti-angiogenic activity were evaluated to determine SBL's potential for skin health improvement. Results showed that SBL exhibited superior antimicrobial activity, with inhibition zones 6.5 % larger than commercial market treatments. SBL also outperformed individual components, demonstrating a 142 % higher efficacy against <em>S. aureus</em> and a 138 % improvement against <em>P. acnes</em> compared to turmeric extract alone. The formulation inhibited trypsin, collagenase, and elastase by 60.81 %, 85.87 %, and 83.58 %, respectively, and inhibited lipid peroxidation by 53.05 %. In the angiogenesis inhibition assay, SBL significantly reduced blood vessel formation by 83.48 %, surpassing market standards and other test treatments. In conclusion, the SBL formulation presents a promising natural solution for dermatological conditions, offering strong antimicrobial, anti-inflammatory, and anti-angiogenic effects. The synergy between probiotics and turmeric extract enhances therapeutic outcomes, supporting the potential integration of SBL into skincare formulations as a safe and effective alternative to conventional treatments.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"6 ","pages":"Article 100242"},"PeriodicalIF":0.0,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leishmaniasis: A multifaceted approach to diagnosis, maladies, drug repurposing and way forward

The Microbe Pub Date : 2025-01-17 DOI: 10.1016/j.microb.2025.100239

Naureenbanu Makarani , Krupanshi Bharadava , Aviral Kaushik , Ayush Dave , Ajit K. Gangawane , Radhey Shyam Kaushal

{"title":"Leishmaniasis: A multifaceted approach to diagnosis, maladies, drug repurposing and way forward","authors":"Naureenbanu Makarani , Krupanshi Bharadava , Aviral Kaushik , Ayush Dave , Ajit K. Gangawane , Radhey Shyam Kaushal","doi":"10.1016/j.microb.2025.100239","DOIUrl":"10.1016/j.microb.2025.100239","url":null,"abstract":"<div><div>More than 20 species of the protozoan parasite Leishmania cause leishmaniasis, a disease spread by sand flies. The WHO lists leishmaniasis as one of the most ubiquitous neglected tropical illnesses. As per the reports, more than 1.3 million cases worldwide result in 20,000–30,000 fatalities every year. Although the global burden of disease is not rising, there remains a chance that it could spread. Leishmaniasis is predominantly detected in Brazil, Ethiopia, India, Kenya, Somalia, South Sudan, and Sudan, with transmission occurring through bites from infected phlebotomine sand flies to humans and other hosts. Pathogenesis of leishmaniasis based on the pathogen or host mechanism. The diagnosis of leishmaniasis is distinguished by clinical pleomorphism confirmation and also done by parasitological methods which are highly specific and sensitive. Despite the favoring of drugs such as Amphotericin B, sodium stibogluconate IV and meglumine IM (antimonial), miltefosine, paromomycin, and pentamidine, specific treatments, medications, and vaccination are still lacking for this neglected disease. Consequently, the drug repurposing-based approach has been adopted to fill this gap. The control measures vary from region to region. This review article describes the epidemiology, clinical aspects featuring the diagnostic and therapeutic techniques along with the benefits and challenges of repurposing drugs. Drugs of various categories have been repurposed to treat leishmaniasis, for instance, Clotrimazole, Nystatin (antifungal); Escitalopram, Imipramine (antidepressant); Paromomycin, Pentamidine (antibiotics); Sunitinib, Lapatinib (anticancer) and many more. The study focuses on the necessity of thorough validation, pharmacological constraints, and regulatory requirements by utilizing advances in computational biology and screening, which can be used as a promising treatment.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"6 ","pages":"Article 100239"},"PeriodicalIF":0.0,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolites profiling based antioxidant, anti-cancer cell proliferation activity and molecular docking and stimulation studies of Parthenium hysterophorus L.

The Microbe Pub Date : 2025-01-17 DOI: 10.1016/j.microb.2025.100238

Vellaiyan Sathish , Ekambaran Gayathiri , Thangaraj Pratheep , Palanisamy Prakash , Subramaniam Umavathi

{"title":"Metabolites profiling based antioxidant, anti-cancer cell proliferation activity and molecular docking and stimulation studies of Parthenium hysterophorus L.","authors":"Vellaiyan Sathish , Ekambaran Gayathiri , Thangaraj Pratheep , Palanisamy Prakash , Subramaniam Umavathi","doi":"10.1016/j.microb.2025.100238","DOIUrl":"10.1016/j.microb.2025.100238","url":null,"abstract":"<div><div>Medicinal plants (MPs) are gaining significance owing to their wealth of bioactive phytochemicals<em>. P. hysterophorus</em> L. is a belonging to the Asteraceae family. The wide range of bioactive substances has led to a greater need for medicinal plants for the treatment of different ailments, including inflammatory conditions, diabetes, and cancer. The plant antioxidant activity in the DPPH and ABTs assays, along with encouraging as assessed by the anti proliferative assay CADD based studies. Moreover, in this study, we highlighted the importance of in silico analysis, which evaluates the anticancer effectiveness of the experiment examines its pharmacokinetics based on molecular docking and dynamics. Phytocompunds derived from <em>P. hysterophorus</em> L were subjected to molecular docking (EGFR and cytochrome p50) complex in cancer. The docking binding scores, with the most favorable score recorded at -9.2 kcal/mol. Molecular stimulation stability of the ligand-protein complexes was supported throughout the simulation. Based on this investigation, these ligands showed promising anticancer activity. Moreover, a thorough examination of laboratory and computational data indicated that the leaf has the potential to be a strong medicinal substance and might be used as a successful treatment in the future.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"6 ","pages":"Article 100238"},"PeriodicalIF":0.0,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteome profiling of human lung epithelial cells unveils distinct patterns of protein expression in response to SARS-CoV-2 ORF3a

The Microbe Pub Date : 2025-01-16 DOI: 10.1016/j.microb.2025.100237

Apoorva , Astha Shukla , Atul Kumar , Saurabh Singh , Sunit K. Singh

{"title":"Proteome profiling of human lung epithelial cells unveils distinct patterns of protein expression in response to SARS-CoV-2 ORF3a","authors":"Apoorva , Astha Shukla , Atul Kumar , Saurabh Singh , Sunit K. Singh","doi":"10.1016/j.microb.2025.100237","DOIUrl":"10.1016/j.microb.2025.100237","url":null,"abstract":"<div><div>The COVID-19 pandemic caused by SARS-CoV-2 remains an ongoing global health concern. Understanding the roles of SARS-CoV-2 accessory proteins is essential for developing effective therapeutic strategies. We present a comprehensive proteomic analysis of human lung epithelial cells in response to SARS-CoV-2 ORF3a. Through, the high-resolution mass spectrometry analysis, we observed significant alterations in the cellular proteome of human lung epithelial cells transfected with SARS-CoV-2 ORF3a. We identified a total of 56 proteins with differential expression patterns. Among these, 17 proteins showed upregulation, while 39 proteins exhibited downregulation in response to SARS-CoV-ORF3a. The pathway enrichment analysis led to the identification of proteins like NEU1, CTSH, ABCE1, Nup155, and KLC1, having important roles in innate immunity, adaptive immunity, cytokine signaling, and antiviral mechanisms. Furthermore, the protein-protein interaction (PPI) network analysis revealed USP10, HELZ, and EXOSC4 as hub proteins. These proteins may play a role in modulating the host's cellular immune functions in response to SARS-CoV-2 ORF3a. Taken together, the present study demonstrates key proteins involved in ORF3a-mediated dampened immune response in human lung epithelial cells, which may facilitate viral replication. These findings may pave the way for understanding the molecular pathogenesis of SARS-CoV-2 and developing novel therapeutic interventions targeting viral accessory proteins.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"6 ","pages":"Article 100237"},"PeriodicalIF":0.0,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of multiplex PCR assay for detection of mycotoxigenic Fusarium species contamination in stored maize grains, India

The Microbe Pub Date : 2025-01-15 DOI: 10.1016/j.microb.2025.100240

Vishwambar Digambar Navale , Koteswara Rao Vamkudoth

{"title":"Development of multiplex PCR assay for detection of mycotoxigenic Fusarium species contamination in stored maize grains, India","authors":"Vishwambar Digambar Navale , Koteswara Rao Vamkudoth","doi":"10.1016/j.microb.2025.100240","DOIUrl":"10.1016/j.microb.2025.100240","url":null,"abstract":"<div><div>This investigation aimed to improve the detection of key mycotoxigenic <em>Fusarium</em> species to address agricultural biosecurity and food safety threats in stored maize grains in India. We developed a multiplex polymerase chain reaction (mPCR) assay for the selective detection of <em>Fusarium</em> species producing mycotoxin, fumonisin B<sub>1</sub> (FB<sub>1</sub>), zearalenone (ZEA), and deoxynivalenol (DON) using their biosynthetic pathway genes viz., FUM1, FUM13, PKS4, PKS13 and TRI13, TRI7, respectively. The mPCR assay demonstrated a limit of detection (LOD) ranging from 1 × 10<sup>3</sup> and 1 × 10<sup>2</sup> spores/g maize grain in both naturally contaminated and <em>Fusarium</em> spores spiked samples, respectively. Maize grain samples were also evaluated for the assessment of mycotoxigenic <em>Fusarium</em> species contamination by mPCR which resulted in A total of 53 maize grain samples (75 %) positive for mycotoxin chemotypes upon mPCR analysis. Among these, 40 sample tested positive for FB<sub>1</sub>, 39 for ZEA and 25 for DON. The levels of contamination ranged from 4 to 6456 µg/kg for FB<sub>1</sub>, 30–2192 µg/kg for ZEA and 5–1713 µg/kg for DON as determined by high-performance liquid chromatography (HPLC). Furthermore, the developed mPCR results exhibited a significant correlation (>95 %) with HPLC data confirming this assays reliability. This finding infers developed mPCR assay is an expeditious, cost-effective, sensitive and selective tool for detecting toxigenic <em>Fusarium</em> species. This method can play a crucial role in assessing food safety and public health, particularly in regions where maize contamination by mycotoxins is a significant concern.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"6 ","pages":"Article 100240"},"PeriodicalIF":0.0,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of atrazine by composite mycelium LMY01: Functions, preparation feasibility and effects on soil microbial communities

The Microbe Pub Date : 2025-01-15 DOI: 10.1016/j.microb.2025.100241

Shengchen Zhao, Jihong Wang

{"title":"Biodegradation of atrazine by composite mycelium LMY01: Functions, preparation feasibility and effects on soil microbial communities","authors":"Shengchen Zhao, Jihong Wang","doi":"10.1016/j.microb.2025.100241","DOIUrl":"10.1016/j.microb.2025.100241","url":null,"abstract":"<div><div>The utilisation of microorganisms for the remediation of environmental contamination represents an efficacious and non-polluting methodology. In this study, two strains of atrazine-degrading bacteria were cultivated in a composite mycelial sphere, designated LMY01, using a co-culture method. The optimisation of the degradation function using an artificial neural network demonstrated that the highest degradation rate of atrazine was 88.62 % within a five-day period. The metabolites of strain LM01 were observed to significantly promote the growth of strain MY01, with the OD600 of strain MY01 increasing by 126.29 % in comparison to the CK treatment following the addition of a sterile culture medium from strain LM01 that had been cultured for 72 h. The docking of the principal functional proteins of the strains demonstrated that proteins with higher binding energies to each other did not directly correlate with atrazine degradation, thus further validating the feasibility of the mycelial-seeking preparation. The results of the microbial diversity analysis indicated that the introduction of the strains resulted in an increase in the abundance of the <em>Actinobacteriota</em> and <em>Acidobacteriota phyla</em>, which may contribute to the degradation of atrazine.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"6 ","pages":"Article 100241"},"PeriodicalIF":0.0,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofilms: A cause for the development of cancer: A review

The Microbe Pub Date : 2025-01-13 DOI: 10.1016/j.microb.2025.100236

Avi Gupta , Debasmita Bhattacharya , Sumitha Elayaperumal , Yuvaraj Sivamani , Dibyajit Lahiri , Moupriya Nag

{"title":"Biofilms: A cause for the development of cancer: A review","authors":"Avi Gupta , Debasmita Bhattacharya , Sumitha Elayaperumal , Yuvaraj Sivamani , Dibyajit Lahiri , Moupriya Nag","doi":"10.1016/j.microb.2025.100236","DOIUrl":"10.1016/j.microb.2025.100236","url":null,"abstract":"<div><div>Cancer is one of the most complex, multifaceted diseases and continues to be the most potent challenge to human health in contemporary history. For centuries, genetic and environmental conditions have been recognized as major contributing factors to this disease, and increasing research has shown that microbial biofilms may play crucial roles in carcinogenesis. Biofilms have been associated with infectious diseases for several decades, but research currently in progress has proven that biofilms play a role in many forms of cancer and may, therefore, be a link between microbial colonization and tumorigenesis. Biofilms stimulate the immune system by releasing proinflammatory cytokines as well as reactive oxygen species. In addition, drug resistance and cancer have been widely attributed to the role of biofilm-forming bacteria. The presence of biofilms in the tumor reduces the degree of immunity and prevents the therapeutic effects of antibiotics and anti-inflammatory drugs. In addition, bacterial resistance to antibiotics can exacerbate issues related to treatment strategies. This need has led to the study of the role played by biofilms in constructing the tumor microenvironment so that novel therapies targeting cancer cells and microbial communities can be discovered. By elucidating the interlinkage between microbial communities and tumors, researchers may uncover new opportunities for early diagnosis, risk stratification, and intervention plans. This review explores the relationship between biofilms and cancer, outlines the mechanisms by which biofilms promote cancer, and discusses implications for cancer prevention and treatment.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"6 ","pages":"Article 100236"},"PeriodicalIF":0.0,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding antibiotic resistance in Pseudomonas aeruginosa: Embracing innovative therapies beyond conventional antibiotics

The Microbe Pub Date : 2025-01-05 DOI: 10.1016/j.microb.2025.100233

Nomita Gupta , Kanchan Chauhan , Garima Singh , Shobhi Chaudhary, Jitendra Singh Rathore

{"title":"Decoding antibiotic resistance in Pseudomonas aeruginosa: Embracing innovative therapies beyond conventional antibiotics","authors":"Nomita Gupta , Kanchan Chauhan , Garima Singh , Shobhi Chaudhary, Jitendra Singh Rathore","doi":"10.1016/j.microb.2025.100233","DOIUrl":"10.1016/j.microb.2025.100233","url":null,"abstract":"<div><div><em>Pseudomonas aeruginosa</em>, an important Gram-negative opportunistic pathogen, is accountable for numerous acute and chronic infections, resulting in mortality rates of up to 40 % and significant morbidity. This opportunistic bacterial pathogen targets individuals with conditions like cystic fibrosis, burn wounds, chronic obstructive pulmonary disorder (COPD), immunodeficiency, cancer, and those experiencing severe infections requiring ventilation, such as in the case of COVID-19. Treatment with antibiotics is currently the only means of preventing <em>P. aeruginosa</em> infections. The pathogens acquired and adaptive mechanisms have led to a rise in the prevalence of multidrug-resistance, which poses a significant challenge in healthcare environments. The bacterial pathogen uses many strategies such as the formation of biofilms, multidrug resistance, and antibiotic tolerance, to spread and maintain infection. The mechanisms of intrinsic and adaptive resistance utilized by this microbe to empower its pathogenesis are covered in great detail in this review. The different antibiotics that are employed in clinical settings to treat <em>P. aeruginosa</em> infections are also discussed. Furthermore, various therapeutic strategies to treat <em>P. aeruginosa</em> infections such as phage therapy and CRISPR-Cas9, antimicrobial peptides, immunomodulators, use of nanotechnology, vaccine development, and the revolutionary concept of drug repurposing are well discussed. Coherently, we draw attention to the research on alternative as well as innovative methods to control <em>P. aeruginosa</em> infections replacing the need of traditionally available antibiotics.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"6 ","pages":"Article 100233"},"PeriodicalIF":0.0,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0