Molecular prevalence of extended spectrum beta lactamase producing Escherichia coli in dressed broiler chickens in Sokoto metropolis

Mukhtar Kabir , Muhammad Sanusi Yahaya , Abdulbariu Ogirima Uhuami , Yusuf Yakubu , Abdurrahman Hassan Jibril
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Abstract

Chickens are a key source of meat in Sokoto, Nigeria. However, excessive antibiotic use in poultry farming has led to antimicrobial resistance (AMR), particularly in Escherichia coli, which can acquire Extended Spectrum Beta-Lactamase (ESBL) resistance genes. This study investigates the molecular prevalence of ESBL-producing E. coli in dressed broiler chickens sold in Sokoto metropolis, Nigeria. A cross-sectional study design was used to collect 165 carcass rinsate samples from broiler chickens using systematic random sampling. E. coli was isolated through culture on MacConkey and Eosin Methylene Blue agar, followed by biochemical characterization using IMViC tests. Molecular confirmation of E. coli was performed by PCR targeting the uidA gene. Confirmed E.coli were subjected phenotypic screening for the production of ESBL using the Double Disk Synergy Test (DDST). Isolates that were phenotypically ESBL producers were subjected to PCR detection of blaTEM, blaSHV, and blaCTX-M resistance genes. Phenotypic screening for ESBL production found 10 cephalosporin-resistant isolates, all of which tested positive using the DDST. Molecular analysis revealed ESBL genes in 33.3 % (9/27) of isolates, with blaTEM detected in 8 (29.6 %), and blaSHV and blaCTX-M in 3 (11.1 %) respectively. One isolate harbored all three genes, three harbored two, and five contained only one type of ESBL gene. The identification of ESBL producing E. coli in poultry that is ready for the market emphasizes the urgent need for molecular surveillance, more stringent laws governing the use of antibiotics in animal husbandry, and greater public education in order to reduce the risks of AMR in food systems.
索科托市肉鸡产广谱β -内酰胺酶大肠杆菌分子流行病学研究
鸡是尼日利亚索科托的主要肉类来源。然而,家禽养殖中过度使用抗生素导致了抗菌素耐药性(AMR),特别是大肠杆菌,它可以获得广谱β -内酰胺酶(ESBL)抗性基因。本研究调查了产esbl大肠杆菌在尼日利亚索科托市出售的肉鸡中的分子流行率。采用横断面试验设计,采用系统随机抽样的方法,采集了165份肉鸡胴体冲洗液样本。通过MacConkey和Eosin亚甲基蓝琼脂培养分离大肠杆菌,并进行IMViC生化鉴定。以uidA基因为靶点,采用PCR方法对大肠杆菌进行分子鉴定。用双盘协同试验(DDST)对确认的大肠杆菌进行表型筛选,以产生ESBL。对表型上为ESBL产生者的分离株进行了blaTEM、blaSHV和blaCTX-M抗性基因的PCR检测。ESBL产生的表型筛选发现10株头孢菌素耐药菌株,使用DDST检测均为阳性。分子分析显示,33.3% %(9/27)分离株中检出ESBL基因,其中8株(29.6 %)检出blaTEM基因,3株(11.1 %)检出blaSHV基因和blaCTX-M基因。一株含有所有三种基因,三株含有两种基因,五株只含有一种ESBL基因。在准备上市的家禽中发现产生大肠杆菌的ESBL,强调迫切需要进行分子监测,制定更严格的法律来管理畜牧业中抗生素的使用,并加强公众教育,以减少食品系统中抗生素耐药性的风险。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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