Pharmacological Research - Reports最新文献

{"title":"In human knee articular chondrocytic-spheroids, Orthogrit modulates TNF-α and IL-1β induced inflammation, oxidative stress, ECM catabolism, and improves locomotory behaviour in Caenorhabditis elegans","authors":"Acharya Balkrishna ,&nbsp;Vivek Gohel ,&nbsp;Nishit Pathak ,&nbsp;Meenu Tomer ,&nbsp;Rishabh Dev ,&nbsp;Anurag Varshney","doi":"10.1016/j.prerep.2025.100050","DOIUrl":"10.1016/j.prerep.2025.100050","url":null,"abstract":"<div><div>Osteoarthritis is a progressive chronic degenerative joint disease characterized by inflammation, pain, and articular cartilage deterioration. The current pharmacotherapies for osteoarthritis do not address the progression of osteoarthritis and are also associated with several adverse effects. Herbal medicines are emerging as potential source for the safe and effective treatment of osteoarthritis. Orthogrit, an herbo-mineral prescription medicine, has anti-oxidant, anti-inflammatory, and cartilage promoting properties that can potentially halt progression of osteoarthritis by its multifaceted bioactivities. The present study aimed to characterize the pharmacological effects of Orthogrit using 3D culture of human knee articular chondrocytes (NHAC-kn) and <em>Caenorhabditis elegans</em>. The chemical characterization of Orthogrit was performed by UHPLC analysis. The <em>in vitro</em> evaluation of Orthogrit was performed on TNF-α and IL-1β co-induced NHAC-kn spheroids; and <em>in vivo</em> analysis on LPS- exposed N2 (wild-type) strain of <em>C</em>. <em>elegans</em>. In chondrocytic spheroids, Orthogrit treatment reduced chondrotoxicity, ROS levels, and release of IL-6, PGE2, and CTXII, a marker of cartilage degradation. Treatment with Orthogrit normalized mitochondrial membrane potential; levels of aggrecan, and sulphated glycosaminoglycans; and reporter activity of IL-1β and NF-κB. In LPS-exposed <em>C</em>. <em>elegans</em>, Orthogrit treatment decreased nematode mortality, ROS levels and normalized locomotory behaviour (reversal and omega turns), SOD, Catalase and GSH levels. The mRNA expression analysis revealed that Orthogrit acts against progression of osteoarthritis by regulating inflammation mediators (<em>JAK2</em>, <em>COX2</em>), catabolic ECM enzymes (<em>MMP1</em>, <em>MMP3</em>, <em>ADAMTS-4</em>), redox homeostasis (<em>Nrf2</em>), p38 MAPK signalling (<em>PMK-1</em>, <em>SEK-1</em>) and apoptosis (<em>CED-3</em>). Taken together, Orthogrit is a potential therapeutic agent for management of osteoarthritis.</div></div>","PeriodicalId":101015,"journal":{"name":"Pharmacological Research - Reports","volume":"4 ","pages":"Article 100050"},"PeriodicalIF":0.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of paraquat-induced toxicity and the therapeutic potentials of Moringa oleifera and Moringa stenopetala leaf alkaloid extracts","authors":"Stephen Adeniyi Adefegha ,&nbsp;Opeyemi Babatunde Ogunsuyi ,&nbsp;Olayemi Philemon Aro ,&nbsp;Ganiyu Oboh","doi":"10.1016/j.prerep.2025.100049","DOIUrl":"10.1016/j.prerep.2025.100049","url":null,"abstract":"<div><div>Paraquat (PQ) exposure is a risk factor for Parkinson's disease (PD), motivating treatment investigations. The efficacy of Ayurveda moringa leaf alkaloids in treating Paraquat toxicity was investigated in <em>Drosophila</em> models. The study included 10 fruit fly groups, four groups of which received moringa leaf alkaloid samples alone, one group was Paraquat-induced, and others received both. The experiment was terminated on day 3, after which fly heads and bodies were homogenized. <em>Moringa oleifera</em> and <em>Moringa stenopetala</em> alkaloid extracts were tested on Paraquat-induced flies' survival, biochemical, and genetic markers of PQ-toxicity. Alkaloid extracts significantly improved paraquat-induced fly survivability. Both <em>Moringa</em> species exhibited a stronger inhibition of monoamine oxidase and acetylcholinesterase at 50 mg/ml. Both <em>Moringa</em> species increased tyrosine hydroxylase activity, with PQ+MSL (50 mg/ml) displaying a more pronounced ameliorative effect. In comparison with the Paraquat-induced group, <em>Moringa stenopetala</em> significantly increased both catalase and total thiol levels. At the genetic level, catalase and tyrosine hydroxylase mRNA levels were downregulated in paraquat-induced flies. <em>Moringa stenopetala</em> exhibited a higher upregulation of tyrosine hydroxylase mRNA expression compared to <em>Moringa oleifera</em>, while <em>Moringa oleifera</em> upregulated catalase mRNA expression more than <em>Moringa stenopetala</em>. In conclusion, alkaloid extracts from moringa leaves demonstrated neuroprotective effects against paraquat-induced neurotoxicity in fruit flies. However, <em>Moringa stenopetala</em> exhibited superior medicinal properties compared to <em>Moringa oleifera</em>, as observed from this study. These findings support the antioxidant potential of Moringa extracts in counteracting environmental toxicant-induced stress. Future studies using non-lethal, neurodegeneration-focused Paraquat exposure models may further elucidate their neuroprotective potential.</div></div>","PeriodicalId":101015,"journal":{"name":"Pharmacological Research - Reports","volume":"4 ","pages":"Article 100049"},"PeriodicalIF":0.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping research trends and hotspots of artificial intelligence, and machine learning in medicine and health within the Eastern Mediterranean region‎: A comprehensive bibliometric analysis","authors":"Arwa M. Al-Dekah","doi":"10.1016/j.prerep.2025.100048","DOIUrl":"10.1016/j.prerep.2025.100048","url":null,"abstract":"<div><h3>Background</h3><div>Artificial Intelligence (AI) and Machine Learning (ML) literature on medicine and healthcare within the Eastern Mediterranean Region (EMR) is proliferating, and the volumes of scientific data are getting increasingly complex to analyze. It is essential to comprehensively analyze the current state of research in this area. This study performs comprehensive bibliometric analysis to evaluate the productivity and impact of publications on AI and ML in medicine and health within the EMR and to anticipate future research directions in the field.</div></div><div><h3>Methods</h3><div>The literature between 1996 and 2024 was retrieved from Scopus and Web of Science (WoS) databases. Microsoft Excel, the R package \"bibliometrix\" and VOSviewer were employed to perform bibliometric analysis and to map the landscape research, identify key themes, and explore trends.</div></div><div><h3>Results</h3><div>A total of 2365 eligible publications were identified, showing an average annual growth rate of 22.92 % over the past two decades. Iran (37.1 %), Saudi Arabia (25.1 %) were the most productive countries in this field. The author with the most publications was Leili Tapak. The journal with the most publications was Scientific Reports, and the most active affiliation was Tehran University of Medical Sciences. The most frequent keywords were \"<em>machine learning</em>\", \"<em>COVID-19</em>\", and \"<em>artificial intelligence</em>\".</div></div><div><h3>Conclusion</h3><div>AI/ML research in the EMR is expanding rapidly, driven by a few high-output countries and concentrated around clinical and diagnostic themes. The findings underscore the need for enhanced intra-regional collaboration, strategic investment in AI infrastructure, and the inclusion of underrepresented countries to ensure equitable AI development across the region.</div></div>","PeriodicalId":101015,"journal":{"name":"Pharmacological Research - Reports","volume":"4 ","pages":"Article 100048"},"PeriodicalIF":0.0,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acetaminophen and acetaminophen-opioid combination prescribing trends among hospitalized children, adolescents, and young adults with cancer","authors":"Cheryl L. Mathis ,&nbsp;Huong D. Meeks ,&nbsp;Kevin M. Watt ,&nbsp;Luke D. Maese ,&nbsp;Jonathan E. Constance","doi":"10.1016/j.prerep.2025.100047","DOIUrl":"10.1016/j.prerep.2025.100047","url":null,"abstract":"<div><div>Acetaminophen (<strong>APAP</strong>) is a ubiquitous antipyretic and analgesic used in children in the United States (<strong>US</strong>), including those with cancer. The effects of US Food &amp; Drug Administration (<strong>FDA</strong>) guidance on APAP prescribing have been described for healthy adults and children; however, APAP use patterns in neonates, infants, children, adolescents, and young adults (<strong>CAYA</strong>) with cancer are unknown. Considering their increased risk of liver injury, APAP’s potential for causing hepatoxicity, and FDA guidance changes, this study examined the recent evolution of APAP use in CAYA with cancer. This retrospective, multi-center analysis extracted APAP prescribing data from the Pediatric Health Information System® (<strong>PHIS</strong>). Eligible patients were aged 0–26 years, had a cancer diagnosis per International Classification of Diseases (<strong>ICD</strong>) codes, and were prescribed a chemotherapeutic. APAP and APAP-opioid combination prescribing were assessed at hospital, regional, and national levels. APAP and APAP-opioid combination use changes were assessed using the non-parametric Mann-Kendall test. PHIS records for the complete years of 2004–2021 yielded 388,364 inpatient encounters for 50,779 unique patients. Of these, 87.3 % of patients received APAP. Although APAP-opioid combination use was infrequent overall, CAYA receiving APAP were more likely to receive APAP-opioid combination medications (N = 25,880, 13.4 %, p &lt; 0.001) compared to those not receiving APAP. Among specialty children’s hospitals, national APAP use was stable over the study period. Regionally, APAP use increased among Northeastern hospitals. APAP-opioid combination use decreased nationally with regional variation. In contrast to the steady decline in other regions, Southern APAP-opioid combination use was consistently elevated before declining in 2014.</div></div>","PeriodicalId":101015,"journal":{"name":"Pharmacological Research - Reports","volume":"4 ","pages":"Article 100047"},"PeriodicalIF":0.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alpha-lipoic acid alleviates testicular dysfunction induced by olanzapine in male rats, involvement of adiposity, oxidative stress, inflammation, and male hormonal factors","authors":"Samir AE Bashandy ,&nbsp;Fatma A. Morsy ,&nbsp;Yousef S. Bashandy ,&nbsp;Marawan A. Elbaset ,&nbsp;Bassim M.S.A. Mohamed","doi":"10.1016/j.prerep.2025.100043","DOIUrl":"10.1016/j.prerep.2025.100043","url":null,"abstract":"<div><div>Olanzapine, classified as an atypical antipsychotic pharmacological agent, has been linked with the onset of metabolic syndrome and reproductive dysfunction. The objective of this investigation was to examine the protective properties of alpha-lipoic acid (ALA) in counteracting the metabolic and reproductive disturbances induced by olanzapine in a rat model. Adult Wistar rats were allocated into four distinct groups: a control group, an olanzapine-treated group (10 mg/kg), and two groups receiving olanzapine in conjunction with alpha-lipoic acid (50 or 100 mg/kg). The treatment regimen was administered over a duration of 8 weeks. The group exposed to olanzapine exhibited statistically significant reductions in luteinizing hormone, testosterone levels, sperm motility, sperm count, and relative weights of reproductive organs, alongside an elevation in inflammatory markers (TNF-α and IL-6), oxidative stress (MDA, SOD and GSH), sperm abnormalities, body weight gain, and fat deposition when contrasted with control subjects. Notably, the concomitant administration of alpha-lipoic acid (at doses of 50 or 100 mg/kg) resulted in a partial, dose-dependent amelioration of the adverse effects induced by olanzapine. Specifically, alpha-lipoic acid (100 mg/kg) alleviated the harmful consequences of olanzapine on reproductive hormones, sperm quality metrics, organ weights, oxidative stress levels, and inflammatory markers. Furthermore, alpha-lipoic acid exhibited advantageous effects on body weight and fat accumulation. These observations indicate that alpha-lipoic acid may serve as a viable adjunctive therapeutic strategy to mitigate the metabolic and reproductive side effects associated with olanzapine administration. Additional investigations are necessary to clarify the underlying mechanisms of these protective effects and their potential clinical ramifications.</div></div>","PeriodicalId":101015,"journal":{"name":"Pharmacological Research - Reports","volume":"3 ","pages":"Article 100043"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potentiation of endocannabinoid signaling alleviates depressive-like behavior in diabetic mice","authors":"Anuradha Kesharwani ,&nbsp;Devidas Lahamge ,&nbsp;Shubhankar Kumar Singh ,&nbsp;Velayutham Ravichandiran ,&nbsp;Vipan Kumar Parihar","doi":"10.1016/j.prerep.2025.100037","DOIUrl":"10.1016/j.prerep.2025.100037","url":null,"abstract":"<div><div>People with poorly managed diabetes frequently feel repressed pleasure and are more prone to developing anxiety and depression. The molecular reasons causing the increased incidence of mood and cognitive impairments after hyperglycemia remain unknown. However, current research suggests that disturbances in the endocannabinoid (eCB) system play a critical role in the initiation and progression of diabetes-related mood and cognitive deficits. Our findings reveal that lower levels of two critical eCB, anandamide (AEA) and 2-arachidonoylglycerol (2-AG), in the medial prefrontal cortex (mPFC) are related to increased anxiety-like and depressive-like behavior in diabetic mice. Additionally, the brains of diabetic mice exhibit elevated levels of Fatty Acid Amide Hydrolase (FAAH), an important enzyme involved in the metabolism of AEA and 2AG, which contributes to the polarization of microglia and the promotion of chronic neuroinflammation. Furthermore, cannabidiol (CBD), a non-psychoactive component of cannabis, administered intraperitoneally for two weeks, improves mood and memory in diabetic mice via transforming the eCB system, as evidenced by higher levels of AEA and 2-AG. The cellular changes induced by CBD treatment in the mPFC included notable modulation of microglia towards a beneficial anti-inflammatory M2 phenotype and a reduction in microglial hypertrophy. CBD therapy resulted in several beneficial molecular alterations in the mPFC, such as a reduction in proinflammatory cytokine levels, an elevation in 2AG and AEA, and a suppression of HMGB1-TLR4 pathway activation. The administration of CBD did not influence the activity of the NAPE-PLD and DAGL-α enzymes, responsible for the synthesis of 2AG and AEA. This indicates that the therapeutic benefits of CBD are not dependent on the enhanced synthesis of AEA and 2AG. Additionally, the administration of CBD significantly reduces fasting blood glucose levels in diabetic mice, indicating that the positive effects of CBD on the brain function of diabetic mice are likely facilitated by enhanced glucose metabolism.</div></div>","PeriodicalId":101015,"journal":{"name":"Pharmacological Research - Reports","volume":"3 ","pages":"Article 100037"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical structures, extraction methods and health benefits of okra-derived polysaccharides (ODPs): emphasis on their antioxidant, antihyperlipidemic and antidiabetic actions","authors":"Ahmed Olatunde ,&nbsp;Abdulazeez Ridwanullah Eyitayo ,&nbsp;Abdulrashid Mohammed ,&nbsp;Suleiman Yusuf Alhaji ,&nbsp;Abubakar Ibrahim Bawa","doi":"10.1016/j.prerep.2025.100038","DOIUrl":"10.1016/j.prerep.2025.100038","url":null,"abstract":"<div><div>Okra-derived polysaccharides (ODPs) are among the most predominant and prominent complex compounds in okra (<em>Abelmoschus esculentus</em> L. Moench) and have caused increasing interest in the area of functional foods due to their wide applications. Although the literature on their pharmacological properties is still scanty, ODPs show structural diversity and related therapeutic actions, including alleviation of diabetes and related disorders including impaired lipid accumulation and cellular oxidation. In support of their therapeutic potential, these complex compounds confer low toxicity and relatively good bioavailability profiles. The extraction methods for these complex carbohydrates have a significant influence on their extraction yields, monosaccharide compositions, structural characteristics and pharmacological properties. In line with this, rhamnogalacturonan I, II and homogalacturonan were reported to be the most common types. The monomers constituting these complex polysaccharides from okra, including arabinose; galactose; galacturonic acid; glucose; glucuronic acid; mannose and rhamnose, were extracted by different techniques such as ultrasound-assisted, microwave-assisted, pressurized water and hot-water extractions. The present review comprehensively highlights the documented preclinical (<em>in vitro</em> and <em>in vivo</em>) studies on the ameliorative roles of ODPs against diabetes, oxidative stress and hyperlipidemia. However, clinical trials of ODPs against several disorders, including diabetes are yet to be documented. Therefore, these compounds are recommended for clinical investigations and more preclinical studies to further unveil their effectiveness and health-promoting functions against diabetes and related impairments such as cellular oxidation and defective lipid accumulations. Also, this will further establish these complex polysaccharides from okra as functional function agents with therapeutic properties.</div></div>","PeriodicalId":101015,"journal":{"name":"Pharmacological Research - Reports","volume":"3 ","pages":"Article 100038"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143888127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alzheimer's disease – An overview of nano herbal formulations","authors":"Vishal Shivaji Patil ,&nbsp;Swapnali Sachin Patil ,&nbsp;Snehal Rajendra Patil ,&nbsp;Akanksha Shahaji Pawar ,&nbsp;Amol Somnath Shete","doi":"10.1016/j.prerep.2025.100042","DOIUrl":"10.1016/j.prerep.2025.100042","url":null,"abstract":"<div><h3>Background</h3><div>The progressive neurological condition known as Alzheimer’s disease (AD) mainly affects elderly persons. Memory loss, cognitive decline, and the build-up of beta-amyloid (Aβ) plaques and neurofibrillary tangles in the brain are some of its distinguishing characteristics. The benefits of current treatment choices are limited, generally fail to effectively stop progression of the disease, and are typically linked to adverse effects that lower quality of life.</div></div><div><h3>Main body</h3><div>Herbal medicines are viable solutions for treating AD, according to recent research, especially when paired with nanotechnology. These formulations seek to overcome important issues with traditional treatments, such as poor permeability and low solubility across the blood-brain barrier (BBB). When used with herbal medicine, nanotechnology provides creative answers to these problems that may improve therapeutic delivery and efficacy. With an emphasis on their use in treatment of AD, this paper examines the relationship between herbal medicine and nanotechnology. It looks at the legal environment and intellectual property rights surrounding herbal nanoformulations, emphasizing need for more thorough research to fully comprehend their modes of action and prospective applications. Additionally, it compiles information on patents and ongoing studies, offering a summary of developments in area.</div></div><div><h3>Conclusion</h3><div>The therapy of Alzheimer's disease can be revolutionized by incorporating herbal treatments into nanostructured compositions. Such advances have the potential to transform treatment approaches by boosting therapeutic results and delivery systems. This review highlights the significance of ongoing research and development in this area, providing promise for improved outcomes and more efficient management of Alzheimer's disease patients.</div></div>","PeriodicalId":101015,"journal":{"name":"Pharmacological Research - Reports","volume":"3 ","pages":"Article 100042"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maresin 2 reduces nociceptive and anxious-like behaviors inhibiting IL-1β in spinal and prefrontal cortex of type 1-diabetic rats","authors":"Gabrielle Oliveira Guilherme ,&nbsp;Matheus Vinicius Ferreira ,&nbsp;Jaderson Pedro Bonfim da Costa ,&nbsp;Bruno Liebl ,&nbsp;Waldiceu Aparecido Verri ,&nbsp;Janaína Menezes Zanoveli ,&nbsp;Joice Maria da Cunha","doi":"10.1016/j.prerep.2025.100045","DOIUrl":"10.1016/j.prerep.2025.100045","url":null,"abstract":"<div><div>Neuropathic pain and anxiety, or depression are common and debilitating complications of diabetes mellitus (DM), with few effective therapeutic options available. Given the significant role of neuroinflammation in the development and persistence of these complications, in addition to the known antinociceptive effects in inflammatory pain models induced by Maresin 2 (MaR2), a specialized pro-resolving mediator, we aimed to explore its therapeutic potential in mitigating mechanical hypersensitivity and anxiety/depressive-like behaviors in chemically induced DM male <em>Wistar</em> rats. Following the induction of experimental type-1 diabetes <em>mellitus</em> (T1DM) using streptozotocin (60 mg/kg, i.p.), rats were treated with MaR2 (1, 3, or 10 ng/rat, i.p.) or vehicle (VEH), starting 14 days after T1DM induction and continuing until the fourth week. Mechanical allodynia was assessed using the electronic Von Frey test (VFT) one day before STZ administration (baseline) and at multiple time points during MaR2 treatment. In the fourth week after STZ induction, behavioral assessments were performed using the open-field test (OFT), elevated plus-maze (EPM), and modified forced swimming test (MFST). Furthermore, samples from the spinal cord (L4-L6), hippocampus, and prefrontal cortex (pFC) were analyzed to measure levels of the pro-inflammatory cytokine IL-1β. MaR2 treatment significantly reduced mechanical allodynia (at all tested doses) and anxiety-like behavior (only at a dose of 3 ng) in diabetic rats, normalizing IL-1β expression in the pFC and spinal cord. However, it did not reverse depressive-like behavior. These results highlight MaR2's potential as a therapeutic agent for pain and anxiety in experimental diabetes, likely via its anti-inflammatory effects.</div></div>","PeriodicalId":101015,"journal":{"name":"Pharmacological Research - Reports","volume":"3 ","pages":"Article 100045"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomes and their roles in major depressive disorder: An overview of the research progress and perspectives","authors":"Mei Tian ,&nbsp;Shuo Yi ,&nbsp;Zhao Li ,&nbsp;Linzhi Li ,&nbsp;Liwen Fang ,&nbsp;Qin Ru","doi":"10.1016/j.prerep.2025.100035","DOIUrl":"10.1016/j.prerep.2025.100035","url":null,"abstract":"<div><div>Major depressive disorder, a debilitating neurological disorder, remains enigmatic in its etiology, and current treatments are still inadequate. Therefore, unraveling its pathogenesis and discovering novel therapeutics is imperative. Exosomes are small extracellular nanoscale vesicles containing biomolecules, such as lipids, proteins, and nucleic acids, and have the ability to facilitate intercellular communication. Their potential as diagnostic indicators and therapeutic agents in conditions like autoimmune diseases, cardiovascular diseases, and cancer has been increasingly recognized. This review briefly introduces the relationship between exosomes and major depressive disorder, providing a systematic overview of how exosomal constituents contribute to the pathogenesis and progression. Furthermore, it underscores the significance of exosome research for advancing diagnostic and therapeutic approaches in major depressive disorder, offering a promising avenue for developing more effective clinical strategies.</div></div>","PeriodicalId":101015,"journal":{"name":"Pharmacological Research - Reports","volume":"3 ","pages":"Article 100035"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}