Pharmacological Research - Natural Products最新文献

{"title":"Erratum to “Evaluation of the effect of milk casein on the pharmacokinetics of rutin” [Pharmacol. Res. Nat. Prod. 5 (2024) 100130]","authors":"Ravindra Semwal , Ankit Kumar , Ruchi Badoni Semwal , Ashutosh Chauhan , Sunil Kumar Joshi , Kumud Upadhyaya , Deepak Kumar Semwal","doi":"10.1016/j.prenap.2024.100131","DOIUrl":"10.1016/j.prenap.2024.100131","url":null,"abstract":"","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"6 ","pages":"Article 100131"},"PeriodicalIF":0.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143156373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of anti-motility and anti-secretory effects of Annona muricata leaves extract in castor oil-induced diarrhoeic rats","authors":"Ndidiamaka H. Okorie ,&nbsp;Cyril C. Adonu ,&nbsp;Charles O. Nnadi","doi":"10.1016/j.prenap.2025.100167","DOIUrl":"10.1016/j.prenap.2025.100167","url":null,"abstract":"<div><div>Diarrhoea is a debilitating gastrointestinal disorder commonly affecting all age groups in the developing world due to lifestyle, hygiene and environmental factors. Most drugs used in the treatment of this disease have side effects and are out of reach in many rural areas. Hence a need to search for more tolerable, affordable and effective anti-diarrhoeal drugs from medicinal plants. The study evaluated the anti-motility and anti-secretory properties and phytochemical constituents of the leaves of <em>Annona muricata</em>. The dried coarse leaves were cold-macerated in methanol (95%v/v) and the resulting extract partitioned successively in <em>n</em>-hexane, ethyl acetate, <em>n</em>-butanol and water to afford their respective fractions using the solvent partition method. Phytochemical screening was conducted using standard methods while acute toxicity of the extract was carried out by the method of Lorke. The anti-motility and antisecretory studies were evaluated using the castor oil-induced diarrhea model in experimental rats. The extraction of the leaves and fractionation of methanol extract yielded 3.5%w/w of the extract and 0.46, 1.45, 0.62 and 0.62%w/w of the <em>n</em>-hexane, ethyl acetate <em>n</em>-butanol and aqueous partitions respectively. The acute toxicity study indicated that there were no unusual behavioural changes and mortality at an administered dose of 5000<!--> <!-->mg of extract per kg of mice. The extract (200 and 400<!--> <!-->mg/kg) exhibited non-dose-dependent anti-diarrheal activity. The extract and its ethyl acetate fraction (200 and 400<!--> <!-->mg/kg) elicited a significant anti-motility activity (p &lt; 0.05) compared to untreated. Pre-treatment of rats with ethyl acetate fraction (400<!--> <!-->mg/kg) significantly inhibited the action of carbachol on gastric emptying (82.0 ± 5.0% vs. 28.1 ± 8.2%),) and gastrointestinal transit (83.5 ± 7.6%, vs 30.10 ± 5.2%) but with no significant effect on the actions of serotonin and metoclopramide. The extract and ethyl acetate fraction of <em>A. muricata</em> demonstrated a significant anti-motility activity which could be mediated by the anticholinergic effect of its phytoconstituents.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"6 ","pages":"Article 100167"},"PeriodicalIF":0.0,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143156376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CHEMICAL COMPOSITION AND PHARMACOLOGICAL ACTIVITIES OF FICUS RACEMOSA FRUIT METHANOLIC EXTRACT","authors":"Harshvardhan Pant ,&nbsp;Sandeep Negi ,&nbsp;Kumud Saklani ,&nbsp;Subhash Chandra ,&nbsp;Lucia Raquel de Lima ,&nbsp;Henrique Douglas Melo Coutinho","doi":"10.1016/j.prenap.2025.100163","DOIUrl":"10.1016/j.prenap.2025.100163","url":null,"abstract":"<div><h3>Background</h3><div><em>Ficus racemosa</em> Linn is a well-known medicinal plant in various traditional systems of medicine and is used to treat kidney stones, biliary disorders, jaundice, diarrhea, inflammatory conditions, liver disorders, hemorrhoids, respiratory and urinary diseases. We investigated the <em>in vitro and in vivo</em> antidiabetic activity, antimicrobial activity, and nutritional profile of the fruit methanolic extract of <em>F. racemosa</em>.</div></div><div><h3>Methods</h3><div>Applying the alpha (α)-amylase and alpha (α)-glucosidase enzymes and disc diffusion methods. Strong antidiabetic effects were seen <em>in vitro</em> and <em>in vivo</em> in the methanolic extract of the fruit portion of <em>F. racemosa</em>.</div></div><div><h3>Results</h3><div>The methanolic extract reduced blood glucose levels to the greatest extent (31.20% <em>in vivo</em> and 68.10% <em>in vitro</em>). The methanolic extract demonstrated potent activity at 16<!--> <!-->mm and 15<!--> <!-->mm against <em>Staphylococcus</em> and <em>Bacillus ceresus</em> with the disc diffusion method.</div></div><div><h3>Conclusions</h3><div>The methanolic crude extracts exhibit strong antidiabetic and antimicrobial activity, especially against <em>Staphylococcus</em> and <em>Bacillus ceresus.</em> Nutrients represent their potent pharmacological applications. The presence of secondary metabolites (flavonoids, alkaloids, phenolics, saponins, and terpenoids) in the crude methanolic extract may cause its antidiabetic and antimicrobial properties.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"6 ","pages":"Article 100163"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143156806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepato-specific activity of andrographolide, a major constituent of Andrographis paniculata (Burm. f.) Wall. ex Nees for targeting liver diseases","authors":"Kallol Roy ,&nbsp;Pankaj Barman ,&nbsp;Saikat Haldar ,&nbsp;Jatin Kalita ,&nbsp;Rituraj Konwar","doi":"10.1016/j.prenap.2025.100155","DOIUrl":"10.1016/j.prenap.2025.100155","url":null,"abstract":"<div><h3>Background</h3><div>Liver diseases accounts for considerable morbidity and mortality worldwide and current standard therapies often suffer from various challenges including inadequate efficacy and detrimental side effects. <em>Andrographis paniculata</em> (Burm. f.) Wall. ex Nees is a seasonal plant endemic to Southeast Asia employed traditionally during in injury, respiratory infections, fever, headache, liver, and other gastrointestinal disorders. Andrographolide, a key active molecule of the plant exhibits broad range of pharmacological activities against various diseases.</div></div><div><h3>Purpose</h3><div>The aim of the review is to present all scientific evidences of traditional uses of <em>A. paniculata</em> and its important phytochemical constituent andrographolide on major liver diseases and other liver complications.</div></div><div><h3>Materials and methods</h3><div>We have collected the previous scientific reports by searching common databases like “Pubmed”, “Google scholar”, “Science direct”, “Research Gate” with primary search common keywords “andrographolide”, “<em>Andrographis paniculata</em>”, “traditional”, “Ethno* ”, “medicine”, “Liver” “Hepat* ” “Diseases” etc. to retrieve maximum possible literature reports. The initially collected reports were further screened for their inclusion based on peer-review status or other authenticity confirmable status of the report and actual relevance. In addition, books, thesis, reports retrieved through Google search engine from other open access sources were also included for further screening and inclusion.</div></div><div><h3>Results</h3><div>The medicinal plant <em>A. paniculata</em> used traditionally since time immemorial for various purposes including liver disorders. Andrographolide is generally tolerable and possibly does not induce significant toxicity. Andrographolide played important role in different pharmacodynamic process such as in oxidative disbalance, lipid peroxidation, inflammation, and disruption of immune response, which are involved in the initiation of liver diseases. It targets various signaling pathways including TRL4/NFKB and TGFβ1/smad2, and p38 MAPK/Nrf2/HO-1 to ameliorate liver diseases. Clinical investigations of <em>A. paniculata</em> and andrographolide alone or in combined form have showed significant potential for their use in liver diseases.</div></div><div><h3>Conclusions</h3><div>Based on all the reported effects of <em>A. paniculata</em> plant gained through traditional clinical studies along with specific pharmacological efficacy of its constituent andrographolide, it can be viewed that there is a considerable potential of this plant for development of new preventive and therapeutic applications against hepatic diseases.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"6 ","pages":"Article 100155"},"PeriodicalIF":0.0,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-cancer potential of hydroethanolic extracts of Kyllinga nemoralis: An in vitro and in-silico studies","authors":"Eunice E. Ampem Danso ,&nbsp;Justice Kumi ,&nbsp;Abigail Aning ,&nbsp;Sherif Hamidu ,&nbsp;Janet Ampofo ,&nbsp;Latif Adams ,&nbsp;Isaac Asiamah ,&nbsp;Francis Ackah Armah ,&nbsp;Alexander K. Nyarko ,&nbsp;Desmond Omane Acheampong","doi":"10.1016/j.prenap.2025.100161","DOIUrl":"10.1016/j.prenap.2025.100161","url":null,"abstract":"<div><h3>Introduction</h3><div>This study aims to investigate the antioxidant and anti-proliferative properties of <em>Kalinga nemoralis</em> to regulate the production and application of herbal preparations.</div></div><div><h3>Method</h3><div>The leaves, roots and the whole plant extracts of <em>Kyllinga nemoralis</em> (KNL, KNR and KNW) were evaluated for antioxidant capacity, flavonoid content (TFC), phenolic content (TPC), in vitro cytotoxicity using Folin-Ciocalteu, Aluminium Chloride colorimetric methods and DPPH (2, 2-diphenyl-1-picrylhydrazyl) and FRAP assays respectively. LC-MS chemical profile was obtained by Agilent HPLC system.</div><div>The results obtained showed that, KNL extracts shown the highest TFC (16421.33 ± 0.06 mg QE/g) followed by KNW (10531 ± 0.02 mg QE/g) and KNR extracts (4741 ± 0.04 mg QE/g) with TFC/TPC ratio of 65.03, 58.50 and 23.75 respectively. The EC₅₀ values of both DPPH and FRAP assays for the extracts ranged from 0.033 ± 0.07–4.10 ± 0.67 mg/mL. KNL and KNR had the strongest ferric reducing activity (EC₅₀ values of 0.88 ± 0.20 mg/mL and 1.52 ± 0.36 mg/mL) while KNW had the least DPPH radical scavenging activity (2.48 ± 0.35 µg/mL). The IC₅₀ value for each extract was greater than 1000 µg/mL, indicating minimal cytotoxicity against PNT2 cell line. KNR and KNW exhibited the strongest inhibitory activity against the PC3 cell line with an IC₅₀ values of 52.65 ± 1.11 and 53.20 ± 0.80 µg/mL respectively. KNL exhibited a good inhibitory activity against the cancer cell line with an IC₅₀ value of 68.54 ± 9.99. The selectivity indices of extracts were greater than 2. LC-MS profile showed four primary peaks representing combined plant constituents, with 12 recognized compounds and five undetermined compounds.</div></div><div><h3>Conclusion</h3><div><em>Kyllinga nemoralis</em> demonstrates antioxidant activity and antiproliferative effects against PC3 cell lines, attributed to its bioactive compounds. Among these, Flufenacet, Diazoxide, and N-(2-Hydroxyphenyl)piperazine exhibited the highest drug-likeness scores with significant bioactivity.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"6 ","pages":"Article 100161"},"PeriodicalIF":0.0,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143156375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical and pharmacological evaluation of Ajuga parviflora Benth","authors":"Pranjli Trivedi ,&nbsp;Shobha Singh ,&nbsp;Ashutosh Yadav ,&nbsp;Divyash Singh","doi":"10.1016/j.prenap.2025.100156","DOIUrl":"10.1016/j.prenap.2025.100156","url":null,"abstract":"<div><h3>Background</h3><div><em>A. Parviflora</em> Benth. commonly known as bugleweed belongs to <em>Lamiaceae</em> family, annual and short lived perennial herb. It is mainly found at higher altitude in hilly areas of Pakistan, China, Malaysia and India. Present study aimed to explore scientific analysis of phytochemical composition and bioactivity of whole plant parts of <em>A. parviflora</em> extract collected from hilly region of Uttarakhand with traditional ethanopharmacological knowledge.</div></div><div><h3>Methods</h3><div><em>A. parviflora</em> Benth. whole plant were collected and different solvent extracts were prepared in aqueous, methanol and hexane by soxhlet followed characterization by LC-MS/MS and determination of total phenolics, total Flavanoid content and antioxidant activity of methanolic extract by 1,1-Diphenyl-2-picrylhydrazyl assay. Extract underwent Cytotoxic and In-vivo Anti-inflammatory activity by Neutral Red Uptake assay and carrageenan induced rat paw edema respectively.</div></div><div><h3>Results</h3><div>Phenolics, flavanoids and tannins in methanolic extract of whole plant parts of <em>A. parviflora</em> were found to be 709.1 ± 0.34 mg GAE/g, 264.74 ± 0.12 mg Rutin E/g and 111.20 mg TAE/g respectively. Furthermore, methanolic extract exhibited moderate antioxidant activity by DPPH method with IC₅₀ values of 51.18 ± 0.56 µg/ml while cytotoxic activity against MCF-7 cell line exhibited remarkable with IC₅₀ values of 4.152 ± 0.52 µg/ml. In-vivo anti-inflammatory activity was also found to be significant. Reverse phase High Performance Liquid Chromatography analysis identified and quantified two compounds i. e. Rutin (1.48 %) and quercetin (1.82 %). Liquid Chromatography-Mass Spectrometry analysis identified seventeen secondary metabolites as major and minor phenolics, flavanoids of methanolic extract. Hence, our finding proves <em>A. parviflora</em> whole plant of methanolic extract might be used as potential antioxidant, anti-inflammatory and anticancer drugs.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"6 ","pages":"Article 100156"},"PeriodicalIF":0.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bergenia ciliata, a Himalayan medicinal herb with Chinese ethnobotanical roots, alleviates L-arginine-induced acute pancreatitis through modulation of inflammation and oxidative stress: Insights from in Silico and in Vivo studies","authors":"Tridip Jyoti Das ,&nbsp;Shaurya Tiwari ,&nbsp;Anjini Bellai ,&nbsp;Upasa Gowala ,&nbsp;Hui Tag ,&nbsp;Kunal Bhattacharya ,&nbsp;Pallabi Kalita Hui","doi":"10.1016/j.prenap.2025.100164","DOIUrl":"10.1016/j.prenap.2025.100164","url":null,"abstract":"<div><div>Acute pancreatitis (AP) is an inflammatory disorder of the exocrine pancreas, where oxidative stress and inflammatory cytokines play a key role in the induction and progression of the disease. The present study aims to examine the in-vivo effect of methanol extract of <em>Bergenia ciliata</em> rhizome (MEBCR) on L-arginine induced AP in mice and to identify novel drug candidates as alternative therapeutic options in the management of acute pancreatitis. Various markers for pancreatic function, inflammation, oxidative stress, and histological parameter were assessed. Our results indicate that mice treated with MEBCR was able to control the L-arginine-induced changes in pancreatic enzymes, oxidative stress markers (MDA, GSH and nitrite), pancreatic inflammatory markers (MPO, IL-1β, TNF-α and IL-6) as well as histological changes in the pancreatic and liver tissues. MEBCR dose dependently decreases the pro-inflammatory cytokines levels such as TNF-α, IL-6, and IL-1β. MEBCR improved the antioxidant defence by improving Nrf-2 and SOD-1 expression. When considered collectively, our findings indicate that MEBCR pretreatment inhibits AP. These outcomes may be related to the antioxidant activity of the antioxidant enzyme, which was brought about by the Nrf2/ARE signaling pathway being activated. Thus, up-regulating the expression of antioxidant enzymes and activating the endogenous antioxidant pathway Nrf2/ARE may represent viable treatment targets for MEBCR during AP. In our <em>in silico</em> analysis, chelidonine demonstrated favourable pharmacological properties and a notable binding affinity of −11.0 kcal/mol towards the Keap1 protein, and the chelidonine-Keap1 complex remained stable through 100 ns simulation with GROMACS without undergoing major fluctuations.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"6 ","pages":"Article 100164"},"PeriodicalIF":0.0,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143156374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute toxicity and antidiabetic potential of moroccan Lavandula mairei essential oil in induced type 1 and type 2 diabetes","authors":"Fatima Ez-zahra Ousaid ,&nbsp;Fouzia Hmimid ,&nbsp;Fatima Abdou-Allah ,&nbsp;Lamiaa Ait Si ,&nbsp;Meryem Souidek ,&nbsp;Fatima Azzahra Lahlou ,&nbsp;Imane Nait Irahal ,&nbsp;Ismail Guenaou ,&nbsp;Chaimae Hilali ,&nbsp;Mehdi Karkouri ,&nbsp;Mostafa Kabine ,&nbsp;Noureddine Bourhim","doi":"10.1016/j.prenap.2025.100162","DOIUrl":"10.1016/j.prenap.2025.100162","url":null,"abstract":"<div><div><em>Lavandula mairei</em> (<em>L. mairei</em>) is a species of plant from the <em>Lamiaceae</em> family that is endemic to Morocco and known for its aromatic and medicinal properties. The aim of the study was to evaluate toxicity and antidiabetic properties of <em>L. mairei</em> essential oil (EO). The toxicity study was conducted on mice by administration of a single oral dose, followed by observation for 14 days. Clinical signs and symptoms associated with each dose administered were closely monitored. Serum biochemical analyses of various biomarkers of hepatic and renal functions, lipid profile, as well as histological sections were examined. Moreover, the antidiabetic activity was evaluated <em>in vivo</em> in type 1 diabetic rats and, <em>in vitro</em> assays were carried out using enzyme suspensions prepared from the liver of type 2 diabetic rats. Additionally, we assessed the ability of the EO to inhibit the enzymatic activity of polyol pathway, α -amylase and α -glucosidase. The biochemical results indicated that the EO was unsafe at a dose of 1000 mg/kg body weight, while histological sections showed clear signs of hepatotoxicity and nephrotoxicity at both 1000 mg/kg and 500 mg/kg. The EO showed a significant antidiabetic potential with a decrease in glycaemia and significant inhibitory effects on some metabolic enzymes. <em>L. mairei</em> EO may be a promising natural remedy for the management of diabetes, but rigorous scientific studies are needed to validate its efficacy and safety.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"6 ","pages":"Article 100162"},"PeriodicalIF":0.0,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143156371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial activity of selected native Australian Terminalia spp. against gastrointestinal pathogens and potentiation of selected antibiotics","authors":"Muhammad Jawad Yousaf Zai ,&nbsp;Matthew James Cheesman ,&nbsp;Ian Edwin Cock","doi":"10.1016/j.prenap.2025.100158","DOIUrl":"10.1016/j.prenap.2025.100158","url":null,"abstract":"<div><div><em>Terminalia</em> species (Family: Combretaceae) have a wide variety of traditional therapeutic, including to treat bacterial infections. However, no previous studies have verified the antibacterial activity of <em>Terminalia petiolaris</em> A. Cunn. Ex Benth. or <em>Terminalia grandiflora</em> Benth. against gastrointestinal pathogens. Similarly, <em>Terminalia ferdinandiana</em> extracts have not yet been evaluated against many gastrointestinal bacteria. Herein, we quantify the minimum inhibitory concentrations (MIC) of <em>T. ferdinandiana</em> leaf and fruit extracts, as well as <em>T. petiolaris</em> and <em>T. grandiflora</em> leaf extracts against several bacterial gastrointestinal pathogens. Aqueous and methanolic <em>T. ferdinandiana</em> leaf extracts exhibited noteworthy inhibitory activity against multiple bacteria, whilst the corresponding fruit extracts displayed good activity against multiple Gram-negative pathogens, but were completely ineffective against <em>B. cereus</em>. The methanolic <em>T. petiolaris</em> extract showed low activity against all pathogens except <em>S. flexneri,</em> against which the activity was moderate. The methanolic <em>T. grandiflora</em> extract was ineffective against all of the bacteria tested. In contrast, all ethyl acetate extracts were effective inhibitors of bacterial growth, except the <em>T. ferdinandiana</em> leaf extract, which potently inhibited <em>A. faecalis</em> growth (MIC = 75 µg/mL). Combining the plant extracts with selected conventional antibiotics substantially enhanced the antibacterial activity of the mixture. Two synergistic combinations, as well as thirty-six additive, fifty-six indifferent, and twenty-four antagonistic combinations were identified. The safety of the extracts was evaluated by screening for toxicity towards human dermal fibroblast cells, with all extracts were classified as non-toxic. The growth inhibitory mechanism(s) of the extracts are discussed, with reference to their phytochemical composition.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"6 ","pages":"Article 100158"},"PeriodicalIF":0.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143156342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-nociceptive activity of Biochanin A- an important isoflavone from natural source","authors":"Shreya R. Savla ,&nbsp;Manisha J. Oza ,&nbsp;Ankit P. Laddha ,&nbsp;Yogesh A. Kulkarni","doi":"10.1016/j.prenap.2025.100157","DOIUrl":"10.1016/j.prenap.2025.100157","url":null,"abstract":"<div><h3>Introduction</h3><div>Biochanin A is an important isoflavone reported in plants such as <em>Trifolium pratense</em>. It has exhibited significant anti-inflammatory activity. The present work was designed to study its effects in animal models of nociception.</div></div><div><h3>Methods</h3><div>Albino <em>Wistar</em> rats were orally administered with Biochanin A, at dose levels of 10, 20, and 40 mg/kg. The anti-nociceptive activity was evaluated using hot plate, tail-immersion, and formalin-induced nociception models.</div></div><div><h3>Results</h3><div>In tail immersion test, maximum tail withdrawal response of animals treated with Biochanin A was observed at dose of 40 mg/kg at 3 h (p &lt; 0.001), which was significantly higher than control animals. In the hot plate test, Biochanin A treated rats showed significant improvement in response time, achieving a peak response at 45 min (p &lt; 0.001) when compared to control animals. Treatment with Biochanin A also showed a significantly decreased nociception in the formalin test (p &lt; 0.001) at doses of 20 and 40 mg/kg.</div></div><div><h3>Conclusion</h3><div>From the results, it can be concluded that Biochanin A has significant effect in management of central and peripheral pain.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"6 ","pages":"Article 100157"},"PeriodicalIF":0.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143201851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}