Pharmacological Research - Natural Products最新文献

{"title":"Chemotypes and bioactivity of essential oils of Croton grewioides Baill","authors":"Caroline Alves Soares ,&nbsp;Itamara Bomfim Gois ,&nbsp;Luís Fernando de Andrade Nascimento ,&nbsp;Arie Fitzgerald Blank ,&nbsp;Roberta Pereira Miranda Fernandes","doi":"10.1016/j.prenap.2025.100374","DOIUrl":"10.1016/j.prenap.2025.100374","url":null,"abstract":"<div><div><em>Croton grewioides</em> Baill. is a medicinal and aromatic plant native to northeastern Brazil, recognized for its diverse biological activities, mainly attributed to its rich phytochemical profile. This review systematically explores the scientific production, extraction methods, chemical composition and biological properties of <em>C. grewioides</em> essential oils (CGOEs), highlighting their therapeutic and agricultural applications. Furthermore, the methodological quality of the prospective studies was evaluated. Twenty-four biological activities were attributed to CGOEs, with predominant pharmacological effects, such as antihepatotoxic, antidepressant, wound healing, anxiogenic, gastroprotective, antimalarial, and vasorelaxant. Estragole was the compound with the greatest predominance among the reviewed studies. Based on the results, we suggest that the species presents five chemotypes (estragole, methyl eugenol, eugenol, anethole, and (<em>E</em>)-anethole), and the compounds estragole and (<em>E</em>)-anethole can be considered chemical markers of this species. This comprehensive review provides essential information for future research with CGOEs in the agricultural, pharmaceutical, and cosmetics sectors, ensuring their authenticity and efficacy.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"9 ","pages":"Article 100374"},"PeriodicalIF":0.0,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering therapeutic potential of phytoconstituents for mitigating cervical cancer: A pharmacognostic approach","authors":"Meghaditya Roy ,&nbsp;Rasna Kumari ,&nbsp;Pooja ,&nbsp;Sahil ,&nbsp;Pooja Yadav ,&nbsp;Neha Jain ,&nbsp;Bapi Gorail ,&nbsp;Vidyullatha Peddireddy ,&nbsp;Tarun Kumar ,&nbsp;Sachin Kumar Singh ,&nbsp;Smriti Ojha ,&nbsp;Manisha Pandey","doi":"10.1016/j.prenap.2025.100355","DOIUrl":"10.1016/j.prenap.2025.100355","url":null,"abstract":"<div><div>Cervical cancer is the second most common cancer affecting women globally, with approximately 570,000 new cases and 311,000 deaths reported annually. The burden is disproportionately higher in low- and middle-income countries, accounting for nearly 90 % of related deaths. Persistent infection with high-risk types of Human Papillomavirus (HPV), particularly 13 oncogenic strains, is a well-established cause of cervical neoplasia. The incidence and mortality are closely tied to the level of healthcare development, with effective screening programs—such as Pap smears, HPV DNA testing, and visual inspection with acetic acid (VIA)—proven to significantly reduce disease burden by detecting precancerous changes early. Prevention through timely screening and vaccination remains key in combating cervical cancer. This review provides a comprehensive overview of the disease's pathophysiology, current treatment strategies and their limitations, the role of phyto-constituents (particularly flavonoids) in therapy, and a summary of recent patents and clinical trials related to cervical cancer.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"9 ","pages":"Article 100355"},"PeriodicalIF":0.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145159975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of antioxidant and immunostimulatory effects of Allium cepa L. skin extracts prepared by distinct extraction methods","authors":"Ji-Su Kim ,&nbsp;Doyoung Song ,&nbsp;Eun-Byeol Lee,&nbsp;Jieun Jung,&nbsp;Hwan-Hee Jang,&nbsp;In-Guk Hwang,&nbsp;Shin-Young Park,&nbsp;Sung-Hyen Lee","doi":"10.1016/j.prenap.2025.100356","DOIUrl":"10.1016/j.prenap.2025.100356","url":null,"abstract":"<div><div>The escalating global demand for sustainable strategies to reduce food waste has intensified interest in the valorization of <em>Allium cepa</em> L. (onion) by-products, particularly onion skins (OS), which are rich in bioactive compounds yet often discarded. Although previous studies have documented the antioxidant and anti-inflammatory properties of OS, systematic evaluations comparing multiple extraction methods under standardized conditions in both <em>in vitro</em> and <em>in vivo</em> models remain scarce. This study addresses that gap by providing the first integrated chemical, functional, and mechanistic comparison of OS extracts prepared by three distinct methods, hot water (HW), 30 % ethanol (30E), and 50 % ethanol (50E) with direct relevance to industrial scalability. UPLC-Q-TOF MS analysis identified quercetin derivatives as the predominant phytochemicals across all extracts. Ethanol-based extracts, particularly 30E, demonstrated superior radical scavenging activity and significantly enhanced nitric oxide production and cytokine secretion in RAW 264.7 macrophages compared with HW. In contrast, HW extract most effectively restored immune function in cyclophosphamide-induced immunosuppressed mice, as evidenced by increased serum IgG, T-cell and B-cell proliferation, COX-2 upregulation, and MAPK pathway activation. The 50E extract induced the highest phosphorylation of NF-κB. These method-specific functional profiles advance the global understanding of OS bioactivity and enable tailored applications in functional food and nutraceutical development. Beyond their mechanistic significance, the results highlight OS upcycling as a globally relevant, sustainable, and health-promoting strategy that supports circular bio-economy initiatives.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"8 ","pages":"Article 100356"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional and health beneficial properties of spinach (Spinacia oleracea L): A comprehensive review","authors":"Arun Kumar Balasubramaniam ,&nbsp;Ganesh Saste ,&nbsp;Azazahemad A. Kureshi ,&nbsp;Vallabh Mulay ,&nbsp;Lal Hingorani","doi":"10.1016/j.prenap.2025.100368","DOIUrl":"10.1016/j.prenap.2025.100368","url":null,"abstract":"<div><div>The goal of this comprehensive review is to synthesize the contemporary scientific knowledge of <em>Spinacia oleracea</em> L. (spinach) through systematic examination of its botanical, nutritional, and therapeutic properties. This nutritionally dense leafy green vegetable is characterized by multifaceted therapeutic properties. The nutritional composition is thoroughly analyzed, emphasizing its rich vitamin profile (particularly folate, vitamin K, and ascorbic acid), mineral content (iron, magnesium, and potassium), and phytochemical constituents, including flavonoids, carotenoids, and phenolic compounds, which serve as the primary bioactive agents responsible for health-promoting effects. The therapeutic potential is systematically evaluated through evidence-based analysis of demonstrated biological activities, including antioxidant capacity, anti-inflammatory mechanisms, anti-neoplastic properties, anti-diabetic effects, hypolipidemic activity, anti-macular degeneration potential, neuroprotective functions, anti-osteoporotic capabilities, hepatoprotective properties, and cardiovascular and gastrointestinal effects. Clinical investigations examining spinach consumption and spinach-derived phytochemicals are critically assessed to establish practical health applications and determine optimal therapeutic dosages. Through comprehensive database searches spanning from inception to 2024, this review provides an exhaustive analysis of spinach's agricultural significance and medicinal value while identifying promising research trajectories in nutraceutical development, biofortification strategies, and functional food applications. By integrating traditional ethnobotanical knowledge with contemporary scientific findings, this review delivers valuable insights for researchers, healthcare professionals, and consumers seeking to understand the nutritional and therapeutic benefits for evidence-based dietary interventions.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"8 ","pages":"Article 100368"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study on the nutritional profile, antioxidant potential, bioactive compounds, and therapeutic benefits of Piper chaba Hunt. and refined Piper nigrum L.","authors":"Samina Yesmin ,&nbsp;Chadni Lyzu ,&nbsp;Nusrat Jahan ,&nbsp;Mehedi Hasan ,&nbsp;Md. Zia Uddin Al Mamun ,&nbsp;Khondoker Shahin Ahmed ,&nbsp;Dipankar Chandra Roy ,&nbsp;Dipa Islam ,&nbsp;Hajara Akhter","doi":"10.1016/j.prenap.2025.100359","DOIUrl":"10.1016/j.prenap.2025.100359","url":null,"abstract":"<div><div>This study examines the proximate composition, mineral content, vitamin C, antioxidant activity, flavonoid content, and pharmacological properties of one of the locally traded species <em>Piper chaba</em> Hunt. with one of the most extensively traded species <em>Piper nigrum L</em>. While both species are integral to traditional medicine and culinary use, their comparative evaluation remains limited. Our findings reveal significant distinctions in their phytochemical profiles and biological properties, underlining their potential for various therapeutic applications. The proximate composition analysis shows that <em>P. chaba</em> has a higher carbohydrate content (75.746 %), contributing to its significant energy value, while processed <em>P. nigrum</em> is richer in protein, which may support gut health and growth. Mineral analysis reveals that refined <em>P. nigrum</em> is notably higher in iron (542.666 mg/kg) compared to <em>P. chaba</em> (210 mg/kg), with <em>P. nigrum</em> containing more potassium, calcium, and magnesium, which play vital roles in electrolyte balance and enzyme functions. Vitamin C content, measured by the iodometric method, shows that <em>P. chaba</em> contains almost double the vitamin C of <em>P. nigrum</em> (0.88 g/gm vs. 0.45 g/gm). Inhibition of oxidation activity, evaluated through DPPH scavenging assays, demonstrates potent activity in both extracts, with <em>P. chaba</em> showing 95.96 % scavenging at 800 µg/mL. Flavonoid and phenolic content analyses indicate significant levels of these compounds, with higher total phenolic content in <em>P. nigrum</em> (85.3 mg QE/g). HPLC-DAD analysis identifies key phytochemicals, including high levels of kaempferol (106.09 mg/100 g) in <em>P. chaba</em> and catechin hydrate (51.56 mg/100 g) in <em>P. nigrum</em> which are significant antioxidant component. Both plants exhibit moderate α-amylase inhibitory activity (43.55 ± o.51 % and 41.41 ± 0.51 %) and significant pancreatic lipase inhibition, with <em>P. chaba</em> showing stronger lipase inhibition (57.07 ± 0.3 %). These findings highlight their potential as natural sources for managing carbohydrate and fat digestion.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"8 ","pages":"Article 100359"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145010065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gomphrena celosioides Mart.: A review of traditional uses, phytochemistry and pharmacology","authors":"Ankita Rani ,&nbsp;Jiten Singh ,&nbsp;Sakir ,&nbsp;Ashok Jangra ,&nbsp;Meenam Bhatia ,&nbsp;Pai S. Raghavendra ,&nbsp;Dinesh Kumar","doi":"10.1016/j.prenap.2025.100366","DOIUrl":"10.1016/j.prenap.2025.100366","url":null,"abstract":"<div><div><em>Gomphrena celosioides</em> Mart. (Family: Amaranthaceae) is a herbaceous plant that is commonly found in India. It has a rich phytochemical profile and diverse ethnomedicinal applications, due to which scientific community showed interest in this plant. Since this plant has long been used in different traditions to treat conditions like fever, bronchial asthma, diabetes, hypertension, jaundice, oliguria, etc., it possesses a lot of potential in developing as a herbal medicine. Phytochemical studies found many bioactive compounds such as aurantiamide, aurantiamide acetate, 4-hydroxybenzoic acid, 4-hydroxy-3-methoxybenzoic acid, stigmasterol, sitosterol, etc. which might be responsible for its use as a medicine. Pharmacological studies have shown its antioxidant, antibacterial, anti-inflammatory, antimalarial, diabetes-fighting, and liver-protecting effects, which in turn back up many of its traditional claims. Although the data available in this plant appears to be reliable but there is a lack of extensive research on its functionality, mechanism of action and safety. This review aims to provide a comprehensive overview of the phytochemistry, pharmacological potential and traditional uses of <em>G. celosioides</em>. The focus will be on the value of the plant in drug discovery and the need for further research to confirm and apply its medicinal characteristics. Also, this information serves as a valuable resource for scientists and researchers who are going to explore this plant for further studies/investigations. By synthesizing existing research and identifying gaps in knowledge, it will act as the foundation for additional investigations that could unlock new medicinal applications of this plant and contribute to the field of natural product drug discovery.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"8 ","pages":"Article 100366"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting GABAA receptor-associated protein using bioactive phytochemicals from Semecarpus anacardium for treatment of epilepsy","authors":"Azar Zochedh ,&nbsp;Yoga Soundarya Mohan ,&nbsp;Kaliraj Chandran ,&nbsp;Mohana Priya ,&nbsp;Naveen Kumar Muthupandi ,&nbsp;Manojkumar Muthupandi ,&nbsp;Naveenkumar Suresh ,&nbsp;Thandavarayan Kathiresan ,&nbsp;Asath Bahadur Sultan","doi":"10.1016/j.prenap.2025.100370","DOIUrl":"10.1016/j.prenap.2025.100370","url":null,"abstract":"<div><div>Epilepsy is one of the most prevalent neurological illnesses, defined by a persistent susceptibility to epileptic seizures. Seizures cause aberrant impulses that disrupt electrical functioning in the brain, resulting in unannounced brain activity. Plant-derived medications have recently received increased attention due to their great efficacy with low toxicity and adverse effects, particularly in the treatment of neurodegenerative diseases. The work focusses on <em>in silico</em> molecular modeling to find novel and more potent antiepileptic phytocompounds from <em>Semecarpus anacardium</em> for the therapeutic management of epilepsy. Sixteen bioactive phytocompounds were identified using a data bank, and their frameworks were obtained from the PubChem directory. GABA_A receptor-associated protein (GABARAP) was utilized as a potent target for screening of antiepileptic agent. For further investigation, a very effective phytochemical based on binding energy was selected according to docking assessment. Molecular docking analysis identified amentoflavone (AME) as the most potent bioactive chemical against the GABARAP (-8.1 kcal/mol). A computational molecular dynamics inquiry confirmed the stability of the GABARAP+AME complex via normal mode analysis. The reactive spots and the structural steadiness of AME was assessed through density functional theory calculation. Further, to authorize drug-likeness belongings of top scored phytocompound AME, <em>in silico</em> pharmacokinetic and toxicity prediction was carried out. The intermolecular interaction measured by dynamic circumstances provided the path for the discovery of the bioactive chemical amentoflavone, which has effective anti-epileptic properties.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"8 ","pages":"Article 100370"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145059848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Underlining the mechanistic insights in managing the malaria through novel nano-therapeutics and herbal drug technology approach: An updated review","authors":"Abin V. Geevarghese ,&nbsp;Hariprasad Ranganathan","doi":"10.1016/j.prenap.2025.100352","DOIUrl":"10.1016/j.prenap.2025.100352","url":null,"abstract":"<div><div>Malaria, which is carried by <em>Plasmodium species</em>, affects billions of people worldwide. On the clinical level, it may vary from being merely asymptomatic to exceedingly severe or even fatal. A few treatment approaches, such as primaquine, lumefantrine, and chloroquine, are currently employed as treatments, as are combinations with artemisinin or its derivatives. There is an urgent demand for additional methods of treatment due to the increasing incidence of poor bioavailability, drug toxicity and malarial drug resistance. To address the current problems, additional research and suggestions based on nano therapeutics and herbal drug technology is need to be addressed. To achieve the this,recent nano technological approaches including Liposomes, Solid lipid nanoparticles (SLN), Nanostructured lipid carriers (NLC),Metalic nanoparticles,Emulsomes which is included in current investigations. This will lead to the development of anti-malarial chemotherapeutic medications for intracellular parasite vacuoles and erythrocytes that were infected with parasites.Due to the major toxic issues associated with anti-malarial medications nano vectored based vaccine design is also a recent development in the field of immunology for malaria. On considering the green nanotechnology, the silver nanoparticles of Neem and Asoka have anti-plasmodial effect will generate a better drug delivery approach for malaria in the upcoming era. Hollow mesoporous ferrite nanoparticles (HMFNs), glucose based gold nanoparticles, lumefantrine phospholipid complex are some the recent advances in the nanotechnological based developments in anti-malarial therapeutics.This review paper deeply analyses the various developments &amp;drawbacks in the area of nanotherapeutics and herbal drug technology for the treatment of malarial parasite.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"8 ","pages":"Article 100352"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144925361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crude Egyptian rosemary essential oil and its encapsulated form protect rats from deoxynivalenol-induced oxidative damage by modulating genes involved in the intrinsic and extrinsic apoptotic pathways","authors":"Mosaad A. Abdel-Wahhab ,&nbsp;Dina Mostafa Mohammed ,&nbsp;Aziza A. El-Nekeety ,&nbsp;Zeinab K. Hamza ,&nbsp;Khaled F. El-Masry ,&nbsp;Amr Farouk ,&nbsp;Sekena H. Abdel-Aziem ,&nbsp;Nabila S. Hassan","doi":"10.1016/j.prenap.2025.100364","DOIUrl":"10.1016/j.prenap.2025.100364","url":null,"abstract":"<div><div>Deoxynivalenol (DON), a mycotoxin, is extremely prevalent and causes severe oxidative damage. This study was conducted to synthesize and characterize Egyptian rosemary essential oil nanoemulsions (EREO-NEs), screen the bioactive compounds in EREO-NEs and the crude oil (EREO), and assess their protective efficacy against the oxidative damage of DON in rats. EREO-NEs were synthesized, characterized, and their chemical compositions evaluated by GC-MS. Six groups of male Sprague-Dawley rats were administered orally for 3 weeks, including the control group, the DON-treated group (5 mg/kg b.w.), and the other groups that received EREO (100 mg/kg b.w.) or EREO-NEs (500 µg/kg b.w.) alone or in combination with DON. Tissues and blood samples were taken for different assays. The synthesized EREO-NEs had an average size of 83.86 nm and a zeta potential of −15.9 mV. The GC-MS identified 14 and 9 compounds that represent 95.93 % and 89.56 % of EREO and EREO-NEs, respectively. DON disturbed liver and kidney function, serum cytokines, tumor indices, oxidative indicators, hepatic mRNA expression of intrinsic and extrinsic apoptotic pathway genes, and histological changes in the liver and kidney. Both EREO and EREO-NE mitigate these alterations, although EREO-NEs were more efficient. This study demonstrates that EREO-NEs are promising for the protection against DON via their antioxidant capacity and modulation of intrinsic and extrinsic apoptotic pathway genes.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"8 ","pages":"Article 100364"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145059846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mucuna atropurpurea seed extract attenuates inflammation by modulation of inflammatory cytokine response and improving the antioxidant system in experimentally-induced carrageenan in rats","authors":"Pratibha Mali ,&nbsp;Viresh Thamke ,&nbsp;Chetan Aware ,&nbsp;Suresh Suryawanshi ,&nbsp;Manali Rane ,&nbsp;Devashree Patil ,&nbsp;Balkrishna Shinde ,&nbsp;Jyoti Jadhav","doi":"10.1016/j.prenap.2025.100358","DOIUrl":"10.1016/j.prenap.2025.100358","url":null,"abstract":"<div><div>The present study used <em>Mucuna atropurpurea</em> seed extract 7 days’ pre-treatment to explore the anti-inflammatory potential in Crgn induced acute paw swelling in rats<em>.</em> The induced inflammation and the formation of oedema were determined by measurement of the paw thickness by digital Vernier caliper. An inflammatory response characterized by oedema, cellular infiltration of neutrophils, and levels of anti-oxidant and pro-inflammatory cytokines imbalance. The findings indicate that MASE pre-treatment significantly modulated the local inflammatory response, as evidenced by a marked reduction in paw oedema in rats. Furthermore, MASE pre-treatment resulted in a substantial attenuation of antioxidant status, highlighting its potent antioxidant properties. Moreover, MASE effectively mitigated the impaired inflammatory response following carrageenan injection, as demonstrated by a reduction in the expression of pro-inflammatory cytokines and mediators. Additionally, MASE enhanced the expression of the anti-inflammatory cytokine IL-10. An acute toxicity assessment was also conducted to evaluate the safety profile of MASE, confirming its safety at the tested doses. Such findings underscore the potential therapeutic benefits of MASE in ameliorating oxidative stress associated with inflammatory conditions.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"8 ","pages":"Article 100358"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144987846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}