Pharmacological Research - Modern Chinese Medicine最新文献

{"title":"Unraveling biomolecules, antidiabetic and antioxidants properties of DelitesTM via pharmacoinformatics and in vitro investigation","authors":"Juan Leonardo ,&nbsp;Purnawan Pontana Putra ,&nbsp;Trina Ekawati Tallei ,&nbsp;Fatimawali Fatimawali ,&nbsp;Nurpudji Astuti Taslim ,&nbsp;Raymond Rubianto Tjandrawinata ,&nbsp;Vincent Lau ,&nbsp;Saad Mustafa ,&nbsp;Miguel A Prieto ,&nbsp;Fahrul Nurkolis","doi":"10.1016/j.prmcm.2024.100551","DOIUrl":"10.1016/j.prmcm.2024.100551","url":null,"abstract":"<div><h3>Introduction</h3><div>Type 2 diabetes (T2D) remains a global health burden characterized by insulin resistance and chronic hyperglycemia, often exacerbated by oxidative stress, leading to severe complications. Despite the efficacy of pharmacological treatments such as metformin, their side effects and costs highlight the need for alternative therapies. Delites™ (Chong Cao Ling Zhi Xiang Tang), a multi-herbal formulation, presents a promising solution with its bioactive compounds including Apocynin, Curcumin, and Quercetin, which are hypothesized to target T2D pathways.</div></div><div><h3>Methods</h3><div>This study employed pharmacoinformatics, in vitro assays, and molecular dynamics simulations to evaluate the antidiabetic and antioxidative properties of Delites™. Metabolomic profiling using Ultra-High-Performance Liquid Chromatography coupled with High-Resolution Mass Spectrometry identified active compounds, while <em>in silico</em> docking analyzed interactions with T2D-related proteins (<em>e.g</em>., KCNJ11, TCF7L2). Enzyme inhibition assays measured alpha-glucosidase and alpha-amylase activity, and antioxidant potential was assessed using ABTS inhibition.</div></div><div><h3>Results</h3><div>Delites™ demonstrated significant enzyme inhibition (EC₅₀ &lt; metformin), strong binding affinity to T2D proteins (e.g., Sachaliside 2: -9.4 kcal/mol with TCF7L2), and antioxidant activity comparable to Trolox (EC₅₀: 54.44 mg/mL). Molecular dynamics confirmed stable interactions of its compounds with target proteins, while network pharmacology highlighted multi-target potential against diabetes-related pathways.</div></div><div><h3>Discussion</h3><div>The findings underline Delites™ as a multi-target therapeutic candidate for T2D management. Its ability to inhibit carbohydrate-hydrolyzing enzymes, interact strongly with key proteins, and mitigate oxidative stress positions it as a holistic alternative. However, further clinical trials are essential to validate these promising in vitro and <em>in silico</em> results, particularly its long-term efficacy and safety.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100551"},"PeriodicalIF":0.0,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of Bidens pilosa alleviates against alcohol—induced hepatic steatosis in rats: In vivo studies and in silico analysis","authors":"Melva Silitonga ,&nbsp;Hudson Sidabutar ,&nbsp;Hendro Pranoto ,&nbsp;Adriana Yulinda Dumaria LumbanGaol ,&nbsp;Feimmy Ruth Pratiwi Sipahutar","doi":"10.1016/j.prmcm.2024.100546","DOIUrl":"10.1016/j.prmcm.2024.100546","url":null,"abstract":"<div><h3>Background</h3><div>Steatosis has become the critical indicator of the higher alcohol exposure to liver injury. Traditional Chinese medicine, notably <em>Bidens pilosa</em>, is used as anti-inflammatory, antihyperglycemic, and hepatoprotective. This current study aimed to investigate the protective effect of <em>Bidens pilosa</em> leaves ethanolic extract (EEB) against alcohol induced hepatic steatosis.</div></div><div><h3>Methods</h3><div>Using the rat model of alcoholic hepatic steatosis (AHS), this study investigated EEB efficacy through the experimental analysis and in silico method. The animals received EEB at doses of 300 and 600 mg/kg for 42 days in the acute alcoholic experiment. Biochemical analysis and histopathological alteration are examined in alcohol-induced liver rats. Additionally, the pharmacological analysis, molecular docking and dynamic study were evaluated.</div></div><div><h3>Results</h3><div>The results indicated that the EEB has ameliorative effects against hepatic steatosis. It significantly improved hepatic histopathological change, the level of blood glucose, triglyceride, LDL and cholesterol in rats exposed to alcohol. Furthermore, the virtual screening study demonstrated the predominant compounds of EEB might regulate the top core targets linked to AHS, particularly AKT/PIK3CA, MAPK, and MTOR, respectively.</div></div><div><h3>Conclusion</h3><div>Thus, the preventive effect of EEB could be an underlying mechanism for the development of herbal medicine to treat AHS in clinical therapy.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100546"},"PeriodicalIF":0.0,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acorus gramineus extract decreases cancer stem cell properties and stimulates ROS signaling pathway in HepG2 hepatocellular carcinoma cells","authors":"Van Hung Hoang ,&nbsp;Thi Thanh Huong Le ,&nbsp;Phu Hung Nguyen ,&nbsp;Viet Hoang ,&nbsp;Van Khang Pham ,&nbsp;Thi Kieu Oanh Nguyen ,&nbsp;Dinh Quang Hung Can","doi":"10.1016/j.prmcm.2024.100548","DOIUrl":"10.1016/j.prmcm.2024.100548","url":null,"abstract":"<div><h3>Background</h3><div><em>Acorus gramineus</em> is called “Shi Chang Pu'” in Chinese and has been used in traditional Chinese medicine (TCM) for over 2,000 years. The records in the 'Shennong Bencao Jing' show that many species in the genus <em>Acorus</em> are used to treat various diseases in humans such as epilepsy, cardiovascular issues, abdominal pain, memory loss, etc. Several species of the genus <em>Acorus</em> have been demonstrated to exhibit antiproliferative effects against various cancer cell lines. This study aims to evaluate the inhitory effects of <em>Acorus gramineus</em> extract (AGE) on HepG2 liver cancer cells.</div></div><div><h3>Methods</h3><div>The inhibitory effect of AGE on HepG2 cell proliferation was assessed using the MTT assay. Migration was analyzed using the wound healing technique. The 3D culture technique was employed to evaluate tumorsphere formation and the invasive potential of cancer cells. Real-time PCR was used for mRNA expression analysis. Changes in the expression levels of CD44 protein and ROS were analyzed using fluorescence staining techniques. Chemical compounds of AGE were identified using UPLC-QToF-MS.</div></div><div><h3>Results</h3><div>The results indicate that AGE exhibits potent inhibitory activity against cell proliferation, with an IC50 value of 34.1 mg/mL. AGE significantly inhibited both the formation and growth of tumorspheres in 3D cultures, reducing both their number and size. Additionally, AGE was found to suppress the invassion of cancer cells. AGE regulated the downregulation of stem cell-related markers including NANOG, SOX2, OCT4, and ALDH. AGE was also shown to increase the production of reactive oxygen species (ROS) within cells by activating genes involved in ROS signaling pathways, which may lead to a decrease in cell proliferation, migration, and invasion, as well as a reduction in cancer stem cell properties. Finally, 58 compounds present in AGE were identified by UPLC-QtoF-MS analysis, and Mulberrofuran K, Akebonoic acid, and Shancilin were evaluated for their affinity with protein markers related to liver cancer stem cells.</div></div><div><h3>Conclusion</h3><div>This study is the first report on the inhibitory activity of AGE against cancer stem cell characteristics in HepG2 cells, highlighting the potential of this plant species as an anti-liver cancer agent.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100548"},"PeriodicalIF":0.0,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gu Sui Bu (Drynaria fortunei J. Smith) prevents osteoporosis in ovariectomized rats by inhibiting pyroptosis through NLRP3/GSDMD/CASPASE-1","authors":"Hui su ,&nbsp;Jun Dong ,&nbsp;Luyao Liu ,&nbsp;Zechen Yan ,&nbsp;Rujie Zhuang ,&nbsp;Guangxin Huang ,&nbsp;Haipeng Xue ,&nbsp;Zhanwang Xu ,&nbsp;Yu Pan","doi":"10.1016/j.prmcm.2024.100544","DOIUrl":"10.1016/j.prmcm.2024.100544","url":null,"abstract":"&lt;div&gt;&lt;div&gt;Traditional Chinese medicine Gu Sui Bu (&lt;em&gt;Drynaria fortunei J. Smith&lt;/em&gt;), has the effect of tonifying the kidneys and strengthening bone. There are many modern studies on the anti-osteoporosis pharmacological mechanism of Gu Sui Bu (&lt;em&gt;Drynaria fortunei J. Smith&lt;/em&gt;) but no reports on the pharmacological mechanism of Gu Sui Bu (&lt;em&gt;Drynaria fortunei J. Smith&lt;/em&gt;) improving cell pyroptosis and anti-osteoporosis have been found.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Aim&lt;/h3&gt;&lt;div&gt;This study aims to verify the changes in cellular standard indicators in postmenopausal osteoporosis, thereby revealing the participating mechanism of pyroptosis and the intervention effect of Gu Sui Bu (&lt;em&gt;Drynaria fortunei J. Smith)&lt;/em&gt; .&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;Gu Sui Bu (&lt;em&gt;Drynaria fortunei J. Smith&lt;/em&gt;) subjected to UHPLC-Q-Orbitrap-MS/MS analysis, and the OVX rat model was constructed in vivo as the research object. It was divided into sham operation group (SHAM), ovariectomized osteoporosis model group (OVX) and Gu Sui Bu (&lt;em&gt;Drynaria fortunei J. Smith&lt;/em&gt;) group (TFRD-L, TFRD-H). After 3 months of modeling, the medication group was treated with Gu Sui Bu (&lt;em&gt;Drynaria fortunei J. Smith&lt;/em&gt;) and the samples were collected after 12 weeks of intervention. ELISA was used to detect the levels of Caspase-1, NLRP3, GSDMD, IL-1β, and IL-18 in rat serum; the right femur was taken for Micro-CT large bone microstructure scanning and femoral BMD detection; the femur was subjected to rat histopathology HE, TRAP staining; immunohistochemistry and immunofluorescence staining of rat histopathology were performed. WB and PCR were used to observe the expression of Osteoblasts and pyroptosis-related indicators Caspase-1, NLRP3, GSDMD and RUNX2, IL-1β, and IL-18.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;UHPLC-Q-Orbitrap-MS/MS analysis the main compounds in Gu Sui Bu (&lt;em&gt;Drynaria fortunei J. Smith)&lt;/em&gt; samples were identified. These 9 chemical components are Palmitic acid, Fisetin, Caffeic acid, Naringin, Rutin, Uridine, Cafestol, Astilbin . Rat Micro-CT, The results of HE staining and TRAP showed that compared with the rats in the OVX group, the number of bone trabeculae in the rats in the &lt;em&gt;Gu Sui Bu (Drynaria fortunei J. Smith)&lt;/em&gt; medication group (TFRD-L, TFRD-H) increased, became wider and thicker, and the bone density increased. Continuity increases and bone lacunae decrease. Rat serum ELISA, femoral tissue immunohistochemistry, immunofluorescence staining and WB, PCR showed that compared with the OVX group, Caspase-1, NLRP3, The expression levels of GSDMD and inflammation were reduced (p&lt;0.05), and the expression of osteogenic marker RUNX2 was reduced and increased (p&lt;0.05).&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;div&gt;The traditional Chinese medicine Gu Sui Bu (&lt;em&gt;Drynaria fortunei J. Smith)&lt;/em&gt; can improve the bone density of ovariectomized osteoporosis model rats and significantly enhance the bone microstructure. At the same time, it reduced the expres","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100544"},"PeriodicalIF":0.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico and in vivo evaluations of fisetin and fisetin-loaded nanosuspension on monoamine oxidase inhibition in Aβ(25–35) induced dementia in mice model","authors":"Siti Zaidathul Iman Zolkiffly ,&nbsp;Mizaton Hazizul Hasan ,&nbsp;Siti Azma Jusoh ,&nbsp;Ashok Kumar Janakiraman ,&nbsp;Sathesh Kumar Sukumaran ,&nbsp;Noreen Husain ,&nbsp;Yuslina Zakaria ,&nbsp;Hanish Singh Jayasingh Chellammal","doi":"10.1016/j.prmcm.2024.100547","DOIUrl":"10.1016/j.prmcm.2024.100547","url":null,"abstract":"<div><h3>Background</h3><div>Amyloid beta (Aβ) plaques on the extracellular matrix and intracellular neurofibrillary tangles comprise the key indicative pathology of Alzheimer's disease (AD). Fisetin, an antioxidant bioactive compound having pharmacotherapeutic effects is applied by traditional Chinese medicine (TCM) such as <em>Toxicodendron vernicifum</em> (Chinese lacquer tree), <em>Cotinus coggygria Scop</em>, Gan Shuang granulates and formula of <em>Acacia catechu</em>-<em>Scutellariae Radix</em>. Nevertheless, fisetin has constraints such as low oral bioavailability, insignificant aqueous solubility and high hepatic metabolisms.</div></div><div><h3>Objective</h3><div>This investigation aimed to envisage the effects of fisetin and its optimised nanoformulation on Aβ_(25–35) desirous neurotoxicity in mice through deciphering inhibitory actions against monoamine oxidase A and B (MAO-A and B) enzymes following molecular docking.</div></div><div><h3>Methods</h3><div>Molecular docking with MAO-A and B enzymes were accomplished by AMDock's integrated AutoDock Tools (ADT) scripts. For <em>in vivo</em> studies, fisetin nanosuspension was prepared by nanoprecipitation method and evaluated for standard characterization. 10 and 20 mg/kg of fisetin and 10 mg/kg of fisetin nanosuspension were given once daily to mice for 21 days. On the 15th day, the mice were challenged with Aβ_(25–35) by intracerebroventricular injection (ICV) and behavioural tests (open field and elevated plus maze) were performed on the 20th day. Biochemical and histology were examined in brain tissues.</div></div><div><h3>Results</h3><div>Fisetin docked to the catalytic positions of MAO-A and B, unveiling good binding scores and molecular interactions with amino acid residues for inhibition activities. Fisetin nanosuspension has average particle size (225.4 ± 2.95 nm) with low polydispersity index (0.19) and standard zeta potential (-19.13 ± 1.17 mV). Findings showed that fisetin increased locomotor activity and reduced anxiety-like behaviour. Fisetin and its nanosuspension significantly reduced the concentration of MAO-A (<em>P &lt;</em> 0.01) and MAO-B enzymes suggesting a potential neuroprotection effect in Aβ peptide-induced amnesia in mice.</div></div><div><h3>Conclusion</h3><div>Fisetin with optimized bioavailability, effectively exhibits neuroprotection through molecular interactions of MAO enzymes. Further investigations affidavits the neuroprotection through bidirectional pathways related to biogenic amines and their deamination on Aβ stress conditions.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100547"},"PeriodicalIF":0.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142657243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress on Tripterygium wilfordii Hook f. as a treatment option for neurodegenerative diseases","authors":"Zhong-mou Zhang ,&nbsp;Tian-tian Zuo ,&nbsp;Ming-hui Chen ,&nbsp;Cai-xia Zang ,&nbsp;Yong-jian Wang ,&nbsp;Ya-dan Wang ,&nbsp;Shuang-cheng Ma","doi":"10.1016/j.prmcm.2024.100545","DOIUrl":"10.1016/j.prmcm.2024.100545","url":null,"abstract":"<div><h3>Introduction</h3><div>Neurodegenerative diseases are becoming increasingly widespread as the global population ages. Although neurodegenerative diseases have received continued attention from researchers, there are currently no drugs that can reverse the progression of these diseases. <em>Tripterygium wilfordii</em> Hook f., a traditional form of Chinese medicine, has been widely used to treat many diseases due to its numerous bioactivities and shows considerable potential for the improvement of neurodegenerative diseases.</div></div><div><h3>Methods</h3><div>Articles for this review were searched on Pubmed, Scifinder, CNKI and Web of Science. Keyword included “<em>Tripterygium wilfordii</em> and neurodegenerative disease,” “triptolide and neurodegenerative disease,” “celastrol and neurodegenerative disease,” and “tripchlorolide and neurodegenerative disease.” 178 articles were initially retrieved and 119 articles were cited finally.</div></div><div><h3>Results</h3><div>In this study, we summarize the progress of <em>T. wilfordii</em>, especially its bioactive components such as triptolide, celastrol and tripchlorolide for the treatment of neurodegenerative diseases and review the therapeutic mechanisms involved. This article provides reference guidelines for the use of <em>T. wilfordii</em> to treat neurodegenerative diseases.</div></div><div><h3>Discussion</h3><div>The bioactive components like triptolide, celastrol, tripchlorolide and others extracted from <em>T. wilfordii</em> has enormous potential in the treatment of neurodegenerative diseases. However, the vague pathogenic mechanisms underlying neurodegenerative diseases, as well as toxicity and side effects of <em>T. wilfordii</em> on clinical use still exist. Further research is needed to investigate these issues.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100545"},"PeriodicalIF":0.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditional Chinese medicine in Alzheimer's disease: From the perspective of GSK-3β and Tau hyperphosphorylation","authors":"Mei Wang ,&nbsp;Wendi Huang ,&nbsp;Juan Huang ,&nbsp;Jingshan Shi ,&nbsp;Nanqu Huang ,&nbsp;Yong Luo","doi":"10.1016/j.prmcm.2024.100543","DOIUrl":"10.1016/j.prmcm.2024.100543","url":null,"abstract":"<div><h3>Introduction</h3><div>Alzheimer's disease (AD) accounts for the majority of dementia cases, characterized by a gradual decline in memory and cognitive functions. Traditional Chinese medicine (TCM) has a long history of treating AD and has proposed a series of causes, pathogenesis and treatment methods, and has extremely rich experience in the treatment of AD. TCM believes that deficiency of the spleen, which is the main organ for the body to absorb nutrients, is one of the important causes of AD. Which coincides with the theory that AD is type 3 diabetes in recent years. Therefore, effectively regulating the common targets of AD and diabetes is a potential strategy for treating spleen deficiency type AD.</div></div><div><h3>Method</h3><div>By conducting an in-depth search in PubMed and China National Knowledge Infrastructure (CNKI), using the keywords \" Traditional Chinese Medicine\", \"Alzheimer's Disease\", \"GSK-3β\", \"Tau Protein\" and \"Tau Hyperphosphorylation\". Ultimately, 92 papers were included for review.</div></div><div><h3>Result</h3><div>Glycogen synthase kinase 3 beta (GSK-3β) plays a significant role in the pathogenesis of AD, and numerous <em>in vitro</em> and <em>in vivo</em> experiments have demonstrated that Chinese medicine monomers and compounds can inhibit its activity, reducing the hyperphosphorylation of Tau.</div></div><div><h3>Discussion</h3><div>TCM can affect the activity of GSK-3β through various pathways, thereby reducing the hyperphosphorylation of Tau protein and improving the cognitive function of AD. These studies highlight the potential of TCM in treating AD, but further basic and clinical research is still required to validate their safety and efficacy. It is expected that our review will yield novel insights and a scientific foundation for investigating the underlying mechanisms of AD and developing novel anti-AD therapeutics.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100543"},"PeriodicalIF":0.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142657351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in Chinese phytopharmacology for female infertility: A systematic review of high-quality randomized controlled trials","authors":"Rodrigo Aguiar ,&nbsp;Samantha Gehlen ,&nbsp;Rui Oliveira ,&nbsp;Jorge Magalhães Rodrigues","doi":"10.1016/j.prmcm.2024.100539","DOIUrl":"10.1016/j.prmcm.2024.100539","url":null,"abstract":"<div><h3>Introduction</h3><div>Infertility is a significant reproductive health issue affecting millions worldwide. Traditional Chinese Medicine (TCM), particularly Chinese phytopharmacology, has garnered attention as a potential complementary treatment. This systematic review aims to evaluate the efficacy and safety of Chinese phytopharmacology interventions for female infertility, focusing on recent randomized controlled trials (RCTs).</div></div><div><h3>Methods</h3><div>A comprehensive literature search was conducted in major databases to identify relevant RCTs published between 2019 and July 2024. Studies were assessed for quality, which also served as an inclusion/exclusion criterion. Data extraction focused on study characteristics, interventions, comparators, and outcomes.</div></div><div><h3>Results</h3><div>Nine good-quality RCTs were included, evaluating various Chinese phytopharmacology interventions for infertility. Positive effects were observed in several studies, particularly for the <em>Zishen Yutai</em> pill, <em>Dingkun</em> pill, <em>Erzhi Tiangui</em> granules, and ginger-isolated moxibustion. These interventions demonstrated potential benefits in improving pregnancy rates, embryo quality, and endometrial receptivity.</div></div><div><h3>Conclusion</h3><div>Chinese phytopharmacology interventions show promise in improving fertility outcomes when used in conjunction with conventional fertility treatments. While the findings are encouraging, more research is warranted to confirm the efficacy and safety of these interventions.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100539"},"PeriodicalIF":0.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142657348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effect between vancomycin and traditional Chinese medicine (TCM) herbs against methicillin-resistant Staphylococcus aureus (MRSA) infections","authors":"Prateek Porwal,&nbsp;Satish Kumar Sharma","doi":"10.1016/j.prmcm.2024.100538","DOIUrl":"10.1016/j.prmcm.2024.100538","url":null,"abstract":"<div><h3>Introduction</h3><div>Methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) poses a significant challenge to global health due to its resistance to many conventional antibiotics. Emerging resistance increasingly compromises the efficacy of vancomycin, a glycopeptide antibiotic, which remains a critical treatment option for MRSA infections. Traditional Chinese Medicine (TCM), with its rich history and diverse pharmacopoeia, offers potential complementary therapies. This review explores the synergistic effects of combining vancomycin with TCM to enhance antibacterial efficacy against MRSA.</div></div><div><h3>Method</h3><div>We conducted a comprehensive literature review using databases such as PubMed, Scopus, and Web of Science. Studies included were those that evaluated the combined effects of vancomycin and Traditional Chinese Medicine (TCM) on MRSA in vitro, in vivo, and in clinical settings. Our selection criteria focused on peer-reviewed articles published in the last two decades. We included studies that provided clear methodologies for assessing bacterial inhibition rates, synergy assessments (using methods such as the checkerboard assay and time-kill curves), and mechanisms of action. Additionally, studies were required to detail the specific TCM components used, dosages, and outcomes. We excluded studies that did not specifically assess the combination of vancomycin with TCM, lacked a control group, or did not provide sufficient methodological details for assessing the synergy between the treatments. Data extraction was standardized to include study design, TCM components used, dosages, and outcomes.</div></div><div><h3>Results</h3><div>The review identified several TCM herbs and formulations that exhibited synergistic effects when combined with vancomycin. Notable examples include Huang Lian (Coptis chinensis), which demonstrated enhanced bacterial inhibition through disruption of bacterial cell walls and biofilm reduction. The combination therapy not only improved bacterial clearance but also reduced the required dosage of vancomycin, potentially mitigating adverse effects. Mechanistic studies showed that TCM compounds could make bacterial cell membranes more permeable and stop efflux pumps from working, which raised the concentration of vancomycin inside cells.</div></div><div><h3>Conclusion</h3><div>The synergistic use of vancomycin and TCM offers a promising strategy to combat MRSA infections, potentially addressing the limitations of vancomycin monotherapy. This review highlights the need for further clinical trials to validate these findings and optimise combination protocols. Integrating TCM with conventional antibiotics could lead to more effective, lower-dose treatments, reducing the risk of resistance development and improving patient outcomes. Future research should focus on elucidating the precise mechanisms of synergy and exploring the clinical applicability of these combinations.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100538"},"PeriodicalIF":0.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142657352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota modulation through Traditional Chinese Medicine (TCM) - improving outcomes in Gastrointestinal (GI) cancer prevention and management","authors":"Yuying Wang ,&nbsp;Yu Zeng ,&nbsp;Xiaoli Chen ,&nbsp;Aiping Lu ,&nbsp;Wei Jia ,&nbsp;Kenneth CP Cheung","doi":"10.1016/j.prmcm.2024.100528","DOIUrl":"10.1016/j.prmcm.2024.100528","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Introduction&lt;/h3&gt;&lt;div&gt;Gastrointestinal (GI) cancers, such as colorectal cancer, pancreatic cancer and gastric malignancies, are increasing in prevalence and are notorious for poor prognosis and lack of satisfactory curative therapies. Emerging evidence indicates that the trillions of gut microbiotas in the GI tract play a crucial role in maintaining health and preventing disease, making it a promising target in GI cancer management. Additionally, the longstanding tradition and wisdom of Traditional Chinese medicine (TCM) are appreciated for offering valuable insights to complement current therapies against GI cancers. According to TCM principles, GI disorders are primarily attributed to ‘Qi-deficiency’ and disharmony among organs functions and Yin-yang imbalance. Treatment strategies not only include herbal formulations but also encompass non-pharmacological methods. For instance, well-known remedies such as Gegan Qing Lian Decoctions and Aidi injection have a long-standing history of treating GI disorders. These herbal combinations are considered Qi tonics which address the root causes of GI cancer. Furthermore, Reishi Mushroom and Ginseng are for their immune-supportive properties and ability to enhance overall vitality. Turmeric and Chinese rhubarb are noted for their anti-inflammatory, detoxifying and blood-nourishing effects. TCM also incorporates non-pharmacological interventions such as acupuncture and moxibustion, which may yield synergistic effects. The therapeutic benefits of these approaches, coupled with their symptom-relieving abilities, have provided insights for the potential integration with conventional treatments such as immunotherapy and chemotherapy. Several clinical trials have demonstrated positive outcomes, highlighting the involvement of gut microbiota in mediating the effects of these herbal interventions within the GI environment.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;A comprehensive literature search was conducted using keywords including Traditional Chinese medicines (TCMs), Gastrointestinal (GI) cancers, gut microbiota, dysbiosis, GI disorders. Initially, a total of 300 articles published up to 2024 were identified, of which 180 articles were selected for inclusion in this review based on their relevance to TCM interventions and the modulation of gut microbiota in relation to GI cancer, either directly or indirectly. A variety of study designs including meta-analyses, systematic reviews, randomized controlled trials, and studies conducted on both human and rats are included.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;This review highlights the functions of gut microbiota and the implications of microbial dysbiosis in GI carcinogenesis, specifically focusing on the mechanisms that link disruptions in gut microbial communities to cancer progression. Additionally, it highlights the potential integration of Traditional Chinese Medicine into conventional cancer treatment and management by both pharmacological and non-pha","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100528"},"PeriodicalIF":0.0,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142572474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}